Proteins often need to move between different compartments within cells. To do this they are packaged into transport pods called vesicles. Many trafficked proteins are synthesized in an organelle called the endoplasmic reticulum, or ER; these proteins are transported away from the ER in ‘COPII’ vesicles, which are formed when the COPII proteins assemble on the ER membrane and force it to bulge outward. The bulge pinches off from the ER membrane, forming the vesicle, which can then move to, and fuse with, a different compartment in the cell.

The COPII proteins assemble in a particular order to form the vesicle—Sar1 inserts into the membrane of the ER; Sec23 and Sec24 form an inner coat and capture the proteins that the vesicle will transport; and Sec13 and Sec31 form an outer coat. Although the structures of the coat proteins are known, how they bind to each other—and to the ER membrane—to form vesicles of many shapes and sizes is less well understood. Now, Zanetti et al. show how the inner and outer coat proteins can interact flexibly to accommodate a variety of cargoes.

Zanetti et al. mixed purified Sar1 and COPII coat proteins with artificial membranes in vitro. As in cells, the proteins assembled a coat on the membranes, creating bulges and vesicles of different shapes. These coats were imaged using an electron microscope, and the images were analysed using computational image-analysis methods. In this way, Zanetti et al. produced a detailed 3D view of the assembled coat.

It was found that the inner and outer proteins each arranged to form lattice structures—like fishing nets—which showed flexibility and variability in the way the individual proteins interact, as well as imperfections in the arrangement. Both coats may help to reshape the membrane, and the inner-coat and outer-coat lattices were also found to move with respect to each other. These flexible properties could allow the coat to assemble on membranes with different shapes and curvatures, forming COPII vesicles with distinct sizes and shapes that can carry a range of cargoes.

In eukaryotic cells, newly synthesized proteins are transported from the endoplasmic reticulum (ER) to the Golgi apparatus through the action of the coat protein complex II (COPII). Assembly of COPII coat proteins on the membrane leads to generation of coated membrane vesicles carrying cargo molecules. Vesicle formation proceeds via sequential assembly of the coat components. It is initiated by the small GTPase, Sar1. Upon exchange of GDP for GTP (catalysed by Sec12), Sar1 exposes an N-terminal amphipathic helix that inserts into the outer ER membrane leaflet, promoting curvature (Lee et al., 2005). Sar1 recruits heterodimers of Sec23 and Sec24 to the membrane, thereby forming the inner layer of the COPII coat (Matsuoka et al., 1998). Sec23/24 is an adaptor complex: Sec24 binds transport cargo while Sec23 interacts with Sar1 and recruits Sec13/31 (Miller et al., 2002; Bi et al., 2007). Sec13/31 heterotetramers constitute the outer coat layer, thought to polymerise into cages that enclose the budding membrane (Fath et al., 2007; Stagg et al., 2008). GTP hydrolysis on Sar1, activated by Sec23 and further accelerated by Sec31, completes the cycle by promoting fission of the bud and coat depolymerization (Zanetti et al., 2012).

X-ray crystallography has been used to obtain structural models for all the coat subunits (Bi et al., 2002; Fath et al., 2007). Available structural data for the inner coat is limited to isolated subcomplexes. Progress has been made in understanding how the outer coat subunits assemble into a coat by using single-particle cryo-electron microscopy to derive models of Sec13/31 cages formed in vitro under high salt conditions in the absence of membrane (Stagg et al., 2006, 2008; Bhattacharya et al., 2012; Noble et al., 2012). A comparison of the vertices and edges in cages of different sizes (60–100 nm) has suggested geometrical rules governing outer coat assembly, and indicated regions of flexibility in Sec13/31 that permit envelopment of vesicles with sizes ranging from 60 to 120 nm (Fath et al., 2007; Stagg et al., 2008; Bhattacharya et al., 2012). Nevertheless, a higher degree of flexibility in COPII architecture is needed to explain the ability of COPII to mediate secretion of cargoes such as 300 nm pro-collagen fibres, which are much larger than the 60–100 nm in vitro assembled cages. There is increasing evidence that incorporation of pro-collagen into COPII coated vesicles is a highly regulated process, and that modifications in the outer coat proteins—such as ubiquitination—as well as in timing of GTP hydrolysis and coat recycling may be necessary for COPII ability to coat large vesicles (Saito et al., 2009; Jin et al., 2012; Kim et al., 2012; Venditti et al., 2012). COPII-dependent defects in collagen transport are linked to a genetic syndrome characterized by late-closing fontanels, sutural cataracts, facial dysmorphisms and skeletal defects: Cranio-lenticulo-sutural dysplasia (Boyadjiev et al., 2006).

There are currently no structural data on the COPII coat assembled in its functional state on a membrane, leaving important mechanistic questions unanswered. How do the inner and outer coat components arrange to form a membrane coat? How do they interact with the membrane? How do they interact with each other? Are the existing models for the outer COPII coat cages representative of the complete coat assembled on a membrane? What is the structural basis for COPII ability to transport large or elongated cargoes such as pro-collagen? Here we address these questions. We provide evidence for coordinated assembly of the two layers of the coat on a lipid membrane and suggest how this interplay may effect shape changes essential to the capture of large and unusually-shaped secretory cargo complexes and particles.

To answer the questions above, it is necessary to understand the structural arrangement of the complete coat in its membrane bound form. To this end we have studied a reconstituted COPII assembly reaction (Bacia et al., 2011). We incubated giant unilamellar vesicles (GUVs) with yeast COPII proteins (Sec12, Sar1, Sec23/24, Sec13/31) in the presence of a non-hydrolysable GTP analogue GMP-PNP. We imaged the reaction mixture by cryo-electron tomography, observing that coated membranes of a variety of shapes and sizes were generated. These included round vesicles, either independent or still attached to the donor membrane, as well as curved or extended tubes, reminiscent of those detected budding from ER exit sites in vivo (Mironov et al., 2003; Fromme et al., 2007; Venditti et al., 2012; Figure 1). All of these structures were surrounded by two protein layers indicating that the COPII coat can assemble on membranes with a range of different curvatures. The outer coat appeared in sections as points or lines (Figure 1A), and in grazing slices as a regular arrangement of X-shaped features arrayed to form a rhomboidal lattice (a lozenge pattern) (Figure 1B,C). Other geometries, such as triangles and pentagons, were observed on vesicles and tube ends (Figure 1D). These geometries are similar to those formed when purified Sec13/31 proteins assemble in vitro in the absence of a membrane (Stagg et al., 2006, 2008). The inner coat layer also appeared as a regular array (Figure 1B), implying that Sar1 and Sec23/24 can also assemble to form a lattice.

Figure 1

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The structure of the outer coat

To understand the architecture of the Sec13/31 outer coat we applied reference free, contrast-transfer function (CTF) corrected, subtomogram averaging to solve the 3D structure of the vertex, and of the connecting rods to resolutions of ∼40 Å (Figure 2A–B, Figure 2—figure supplement 1, and ‘Materials and methods’). The vertex structure was a twofold symmetric X-shape, similar to that seen in in vitro assembled Sec13/31 cages (Stagg et al., 2006, 2008) (Figure 2D, Figure 2—figure supplement 2). The connecting rods are consistent in shape and size with Sec13/31 heterotetramers, and are bent in the middle by approximately 15°. This same bend is seen in the solved X-ray crystal structure, which can be fitted into the densities as a rigid body (Figure 2B,C). In contrast, there is a 45° bend in the rods of in vitro-assembled protein cages (Stagg et al., 2008; Figure 2E,F). These data indicate that the central hinge between Sec31 molecules can adapt to assemble coats of different curvatures.

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The published EM reconstructions of in vitro assembled COPII outer coat protein cages reveal that the Sec31 β-propeller domains at the ends of two Sec13/31 rods (referred to in the literature as the plus ends) contact each other directly at the center of the vertex, whereas the ends of the other two rods (the minus ends) contact the plus ends at the side and are more distant from the center of the vertex. The geometrical relationship of the ‘+’ and ‘−’ rod ends at the vertex can be described by two angles: alpha (clockwise between + and − ends, which is 60° in the cages), and beta (clockwise between − and + ends which is variable at least between 90° and 108° in the cages) (Figure 2A,B, Figure 2—figure supplement 2, Figure 3—figure supplement 1) (Stagg et al., 2008). Within the in vitro assembled cages each Sec13/31 rod makes a ‘+’ contact at one end and a ‘−’ contact at the other end. This arrangement allows assembly of a coat that curves in two directions, appropriate for coating spherical membranes. (Figure 3C, Figure 3—figure supplement 1). In our reconstructions, the arrangement of Sec13/31 rods we observed at the rounded tips of tubes and on spherical membranes (Figure 1D) was similar to that seen in in vitro assembled cages, consistent with the presence of such ‘+/−’ rods (Figure 3C). When we analysed the distribution of vertices (Figure 3A) and rods (Figure 3B) on the tubular membranes we found that they assembled regions of rhomboidal lattice. A rhomboidal lattice could be built in two ways: each vertex could be rotated by approximately 90° in the plane of the membrane relative to the adjacent vertices, or each vertex could have the same orientation (see schematic in Figure 3—figure supplement 2C). The final structure of the vertex on the tubes clearly shows the expected twofold features of the vertex previously described by Stagg et al. (Noble et al., 2012), indicating that the majority of vertices have the same orientation (‘Materials and methods’). In this arrangement of vertices the rods oriented in one direction (the right handed rods, purple in Figures 2C and 3B) each make two ‘+’ contacts (+/+), while the left-handed rods (green in Figures 2C and 3B) each make two ‘−’ contacts (−/−). The alpha angle is 79.7° ± 5.9° and is oriented around the tube circumference while the beta angle is 95.7° ± 5.8° and is oriented along the tube axis (Figure 3—figure supplements 2 and 3). Together these data imply that three properties contribute to outer coat adaptability: (i) variability of both alpha and beta angles at the vertices, (ii) flexibility of the central rod hinge, and (iii) the absence of any inherent asymmetry in the Sec13/31 rods, allowing them to make ‘+’ contacts at both ends, ‘−’ contacts at both ends, or a ‘+’ contact at one end and a ‘−’ contact at the other. Together this versatility allows coating of not only spherical, but also of tubular membranes and therefore accommodation of large elongated cargoes such as pro-collagen.

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Proteins able to form both spherical and tubular structures are found in other biological systems, most notably virus capsids. For example, the elongated heads of T-even phages, as well as the capsid cores of retroviruses, are assembled as closed structures built from hexamers and pentamers of the component protein and can have regions with spherical and with tubular curvatures (Baschong et al., 1988; Ganser et al., 1999). This structural flexibility can be understood within the framework of quasi-equivalence: while subunits are found in symmetrically different positions with different local curvatures, the contacts between the subunits are only subtly different (Caspar and Klug, 1962). In some virus capsids, subtle changes in the contacts are supplemented by structural switches that mediate the change from hexameric to pentameric assembly (Johnson and Speir, 1997). It seems likely that flexibility in the outer COPII cage geometry is primarily mediated by subtly varying contacts at multiple hinge positions in the rod and vertex (Noble et al., 2012), but we cannot rule out the presence of structural switches.

The structure of the inner coat

Our images showed that the inner coat can also form a regular lattice, suggesting it may not only function to link cargo and membrane to the outer coat, but also play a structural role in determining vesicle shape. We applied reference-free, CTF corrected, subtomogram averaging to solve the structure of the repeating inner coat unit with a resolution of approximately 23 Å (Figure 4A, Figure 4—figure supplement 1; ‘Materials and methods’ and Faini et al., 2012). The bow-tie-shaped repeating unit interacts tightly with the outer membrane leaflet. The units interact with each other to form an approximately helical array on the tube surface (Figure 4B). In many instances we see step changes in tube diameter, associated with displacement of a few subunits from the lattice (Figure 4B).

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We docked the crystal structures of Sec23/Sar1 (PDB 2QTV), and of Sec23/24 (PDB 1M2V) (Bi et al., 2002) into the subtomogram averaging electron density map. Both fit unambiguously into one bow-tie shaped subunit as rigid bodies, in each case placing Sec23 in the same position (‘Materials and methods’; Figure 5A). Analysis of the fitted models reveals the novel network of interactions that mediate lattice formation and membrane binding. Two interfaces between the long edges of the Sar1/Sec23/24 heterotrimer mediate formation of lattice rows. The larger involves Sar1, the gelsolin-like and the C-terminal domain of Sec23 from one heterotrimer interacting with the Zn-finger and part of the beta barrel domains of Sec23 in the adjacent heterotrimer. The smaller interface is between the Zn-finger domain of Sec24 in one heterotrimer and the gelsolin-like and C-terminal domains of Sec24 in the neighbouring heterotrimer (Figure 5B). These contacts are characterised by extended surfaces with opposite charges, a property that is conserved (Figure 5—figure supplement 1). The rows contact each other where the beta barrel domain of Sec23 approaches the Sec24 N-terminus. In the Sec23/24 crystal structure (PDB ID 1M2V), the Sec24 N-terminal helix 61-73 forms a crystallographic contact with the beta-barrel domain of Sec23 in the neighbouring asymmetric unit (Bi et al., 2002). When placed in this position, the 61–73 helix fills an unoccupied region of electron density in our structure, suggesting this contact is present in the assembled coat (Figure 5A,B, and ‘Materials and methods’).

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Our structure reveals that the concave surface of the inner coat heterotrimer is oriented towards the membrane and the Sec31 binding site towards the coat exterior (Figure 5A,C), as previously predicted (Bi et al., 2002). We identify two primary membrane interacting regions. One is formed by a small part of Sec23 together with Sar1, orienting Sar1 to direct its N-terminal amphipathic helix into the outer leaflet. The other is a large basic surface in Sec24, including the Sec24 Zn finger, beta barrel and alpha helical domains (Figure 5B). While the Sec24 Zn finger domain binds the membrane, the Sec23 Zn finger domain does not, instead it faces the neighbouring heterotrimer, suggesting that the role of the Zn fingers in Sec23 and Sec24 has diverged. Consistent with this difference in function, the Sec24 Zn finger, essential for cell survival (Peng et al., 1999), contains a conserved basic patch that is absent in Sec23. We speculate that, analogous to the basic Zn fingers in FYVE domains (Gaullier et al., 2000), this represents a phospholipid-binding site. The trunk domains of Sec23 and Sec24 do not contact the membrane but are suspended above it, leaving space that would allow binding to more highly curved vesicles (Figure 5B).

Inner coat assembly into lattices must remain compatible with the well-characterised cargo binding activity of Sec24. To test this, we modelled the X-ray structures of Sec24 co-crystallised with cargo-derived peptides (Mossessova et al., 2003; Mancias and Goldberg, 2007, 2008), or the cytosolic domain of a globular cargo Sec22 (Mancias and Goldberg, 2007) in our map. None of the characterised cargo-binding sites overlap with the protein–protein and protein–membrane interfaces we identified in the lattice: all remain accessible to cargo in the assembled inner coat (Figure 5C,D). Strikingly, comparison of the co-crystal structure of Sec23/24/Sec22 with our density map reveals that the space between heterotrimers in the assembled inner coat lattice forms a pocket into which Sec22 would bind (Figure 5C).

We cannot rule out that the large-scale order seen in the inner coat lattice is promoted by carrying out assembly in vitro in the absence of GTP hydrolysis. Three factors suggest, however, that the interactions we observe between heterotrimers are relevant in vivo and are not simply an artefact of in vitro assembly. Firstly, they result from a productive assembly reaction that bends the GUV membrane into a variety of shapes. Secondly, the interfaces have conserved electrostatic properties. Thirdly, the interactions that mediate inner coat lattice assembly are distinct from those that mediate membrane binding, outer coat binding, and cargo binding and can accommodate Sec22 in the correct position: they are fully compatible with available biochemical and structural data.

The relationship between inner and outer coats, and implications for assembly

The low resolution that can be obtained in cryo-EM reconstructions of Sec23/24 bound to in vitro assembled Sec13/31 cages, and the necessity of implying symmetry which may not be appropriate for both coat layers, has prevented a clear understanding of the structural relationship between the inner and outer coats (Stagg et al., 2008; Bhattacharya et al., 2012). Our structure of the outer coat does not reveal density corresponding to an ordered inner coat, and vice versa, indicating that the relative positions of the two coat layers are not fixed. Nevertheless, the two layers are approximately aligned both in spacing and orientation. The ‘rows’ in the inner coat are aligned to the left-handed rods of the outer coat, with four inner coat heterotrimers spanning one outer coat rod. The inner coat ‘columns’ are aligned to the right-handed outer coat rods, with two inner coat heterotrimers spanning one outer coat rod (Figure 3A,B, Figure 3—figure supplement 3). This relationship suggests that the unstructured C-terminal region of Sec31 (Fath et al., 2007; Noble et al., 2012), which connects the inner and outer coats, constrains the coat layers but does not fix their absolute positions, much like multiple anchor cables on a ship. This flexibility in the relative positions of the two layers would permit adaptation of the coat to variable curvatures. Gaps and dislocations in both inner and outer coat lattices may further contribute to curvature variability.

The spherical tube ends have an outer coat arrangement similar to that previously observed in in vitro cages, in which adjacent vertices are rotated relative to one another. A helical array of the inner coat heterotrimer cannot be present on the spherically-curved tips. Instead, we consider it likely that on the tube tips, the inner coat lattice is arranged in smaller patches rotated relative to one another, each maintaining the same relationship to the overlying outer coat vertex. Gaps between or within lattice patches could accommodate cargoes with large membrane-proximal domains. A comparison of our results with previous hypotheses for the relationship between inner and outer coats (Stagg et al., 2008; Bhattacharya et al., 2012) is in Figure 6.

Figure 6

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The structural data we have presented shows that both inner and outer COPII layers have sufficient structural versatility to coat both spherical and tubular membranes, and thereby accommodate important large cargoes such as pro-collagen. We found that both coats can assemble regular lattices with constrained relative positions. This relationship would permit the inner coat to influence the arrangement of the outer coat and therefore to play a critical structural role in determining vesicle shape. Our data hint at the possibility that the formation of larger arrays of the inner coat and the formation of associated rhomboidal outer coat lattices, could favour the formation of larger tubular carriers able to accommodate cargoes such as pro-collagen. It has previously been proposed that proteins such as TANGO1 and Sedlin (Saito et al., 2009; Jin et al., 2012; Kim et al., 2012; Venditti et al., 2012) act in concert to stabilize the inner coat, modulating Sar1 GTPase activity and delaying both outer coat recruitment and scission. We suggest that stabilization of the inner coat could promote growth of larger carriers not only by delaying scission, but also by promoting tubular membrane morphology (Figure 7).

Figure 7

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Thank you for sending your work entitled “The structure of the COPII coat assembled on membranes” for consideration at eLife. Your article has been favorably evaluated by a Senior editor and 3 reviewers, one of whom, Stephen Harrison, agreed to serve as a guest Reviewing editor, and one of whom, Sriram Subramaniam, has agreed to reveal his identity.

The guest Reviewing editor and the other reviewers discussed their comments before we reached this decision, and the following comments have been assembled to help you prepare a revised submission.

This analysis by electron tomography of the complete COPII coat assembled on membranes in vitro offers new insights regarding the disposition of membrane and the inner and outer COPII coat components. The difference between the coats studied in the work reported in this MS by electron cryotomography (cryoET) with subtomogram averaging and those studied previously by single-particle reconstruction is the mode of preparation. These are made by in vitro budding from GUVs; the previous coats were assembled from protein only. The new structures show that Sec13/Sec31 forms tubular lattices as well as the isometric (round) structures seen in the earlier work and that the inner protein layer (Sar1/Sec23/Sec24) packs, in these particles, into a locally ordered lattice, related to the lattice of the outer layer in the tubular regions. The main discovery is that Sec13/31 can form an extended rhomboidal lattice to coat membrane tubes – a variation on the triangle/square/pentagon polyhedra described previously. The results described in the manuscript clearly add to our knowledge of COPII coat structures, and when the manuscript is revised to meet the concerns listed below, it should meet the criteria for publication in eLife. The reviewing editor does wish to emphasize that he will only consider the revision if it includes real modifications that indeed respond to these concerns.

1) It is unclear how much significance should be ascribed to the Sec23/24-Sar1 inner lattice. These proteins have been driven onto membrane by the non-hydrolyzable GppNHp, resulting in their two-fold excess over Sec13/31. The authors describe tantalizing features of this regular array: the electrostatic complementarity of lattice contacts, the nice fit of modeled Sec22 SNARE, the packing of a Sec24 peptide element onto Sec23. But all this might be akin to a description of the lattice of a protein crystal. Some further discussion of this issue is needed, to make it clear that the extensive regularity might be a feature of the in vitro assembly. For example, the authors might consider to what extent cargo with appreciable cytoplasmic mass could fit into these tubular regions if they are coated with such a close-packed Sec23/24-Sar1 lattice.

A minor point in this connection was raised by one of the reviewers. The explanation that is offered for how the Sec13/31 lattice differs on tubes and spheres doesn't seem quite definitive. (The alpha and beta angles are germane, but mention of them tends to confuse as much as to resolve the issue). In the simplest sense, the extended lattice arises because the Sec13/31 rods can assemble into an array of squares (rhomboids). The +/+ and -/- arrangement of rods is not absolutely required for a rhomboidal lattice to form; a +/- arrangement would work, it would retain two-fold vertex centers. Rather, the importance of the +/+ -/- arrangement is presumably that this geometry brings the Sec13/31 lattice into close (though still imperfect) register with the inner Sec23/24-Sar1 lattice. Regarding this point, it isn't clear how continuous the +/+ -/- arrangement really is along the tubular membranes. In the Table of Figure 3 - figure supplement 3, the difference between the cell edges for the left-handed (31.9 nm) and right-handed (30.2 nm) “helices” seems a bit small. It would be useful if the authors could measure this based on the Sec13/31 crystal structure. Could there in fact be occasional dislocations in the +/+ -/- lattice?

2) Tubular variants of round structures were among the earliest coat structures ever examined by electron microscopy - the polyheads of T4 and related bacteriophages, studied in detail by Kellenberger and Klug in the ’60s. Those groups examined different classes of polyheads and suggested how subunit packing might generate them. Recall also that T-even phages actually have elongated heads (because of a “waist” between the two rounded ends, each with hemi-icosahedral symmetry), and giant T4 phages have very long heads (extended tubular waists). More recently, the beautiful work of Sundquist and Finch on cone-like assemblies of the HIV-1 capsid protein illustrates an even more striking polymorphism that generates tapered tubes. So the unwarranted use at the end of the first paragraph in “Results and Discussion” of the word “unexpectedly” (an interjection that should be used sparingly, if at all, in scientific publications) is either an admission of ignorance or an unnecessary attempt to impart “novelty”. If procollagen and other elongated molecules are transported by the COPII pathway, then tubular structures with rounded ends are precisely what we can expect. The structural results presented here are interesting, not because they show tubes with hemispherical ends, but rather because the structure of the tubes is not the only one that could have been imagined from the lattice of isometric coats and because the lattice has a cogent interpretation in terms of the high resolution structures of its components. The authors should add some discussion of the clear and relevant precedents for their results.

3) The authors should pay attention to the notion of quasi-equivalence and the way in which high-resolution structures of icosahedral viruses have given this notion specific instantiations and modified earlier formulations. The authors appear not to appreciate that there is important precedent in work on virus structures, especially Klug's classic studies that followed in the initial work on polyheads. The different conformations for the edges (hinge bending) are quite consistent with similar variations in virus capsid assemblies and polymorphisms. In this respect, the description of edges as “++”, “+-”, etc. is confusing. Designations based on the contacts at the ends, rather than on the conformation of the edge dimer itself, may mislead the reader into thinking that there is some inherent asymmetry in the dimer, rather than an asymmetry in its bonding (which may or may not generate some longer-range asymmetry in its conformation).

4) The model at the end impresses two of the reviewers as unlikely or unnecessary. The packaging of an elongated cargo can drive the switch from hemispherical to tubular, just as some variation of membrane tension or other stochastic fluctuation has presumably driven the switch during the in vitro assembly of the coats studied here. Moreover, there is no reason why the outer-layer and inner-layer proteins cannot co-assemble, with the lattice of the latter imposing regularity on the packing of the former. Positing regulatory proteins that somehow communicate with cargo could send cell biologists on unnecessary wild-goose chases. In the case of elongated phage heads, an internal protein (the scaffold) appears to dictate the formation of a tubular part: it is really akin to cargo in the case of a vesicle. (The phage DNA gets stuffed in later, after the scaffold goes away.) Note that an example of a coat protein that appears not to form tubular structures (i.e., lattices curved in one direction but not the other, instead of lattices with two dimensions of curvature) is clathrin, probably because it is bent at a trimeric vertex, which can flatten (more or less) but not in one direction only. In that case, elongated cargos cause coat assembly to stall, because the shell curves back against the membrane that surrounds the cargo and cannot continue; recruitment of actin then drives further engulfment.

5) Finally, there are some technical aspects of the cryo-electron tomography that the authors should address. The information provided on the subvolume averaging is minimal, so it is not possible to assess the quality of the data. A few representative tomograms of the actual data would help. Further, the use of CTF correction is not well supported. The authors need to show power spectra without CTF correction to show that there was measurable signal beyond the first zero (for data in Figure 4). At 200 kV, and at about 2 micron defocus, the first zero crossing of the CTF is about 22.5 Å, so the claim of resolution improvement with CTF correction is hard to understand. The actual resolution is probably around 30 Å, notwithstanding the FSC plots, which are especially unreliable for defining resolution in sub-tomogram averages. Nevertheless, the mechanistic conclusions could be drawn even if the data were only at resolutions of 30-40 Å.

1) It is unclear how much significance should be ascribed to the Sec23/24-Sar1 inner lattice. These proteins have been driven onto membrane by the non-hydrolyzable GppNHp, resulting in their two-fold excess over Sec13/31. The authors describe tantalizing features of this regular array: the electrostatic complementarity of lattice contacts, the nice fit of modeled Sec22 SNARE, the packing of a Sec24 peptide element onto Sec23. But all this might be akin to a description of the lattice of a protein crystal. Some further discussion of this issue is needed, to make it clear that the extensive regularity might be a feature of the in vitro assembly. For example, the authors might consider to what extent cargo with appreciable cytoplasmic mass could fit into these tubular regions if they are coated with such a close-packed Sec23/24-Sar1 lattice.

The reviewers rightly point out that the extensive regular lattice we observe could be akin to a 2D crystal promoted by the absence of GTP hydrolysis and request further discussion of this issue. The revised manuscript now includes the text below.

In the section “The structure of the inner coat”, starting:

“We cannot rule out that the large-scale order seen in the inner coat lattice is promoted by carrying out assembly in vitro in the absence of GTP hydrolysis…”

In the section “The relationship between inner and outer coats, and implications for assembly”, starting:

“Gaps and dislocations in both inner and outer coat lattices may further contribute to curvature variability…”

A minor point in this connection was raised by one of the reviewers. The explanation that is offered for how the Sec13/31 lattice differs on tubes and spheres doesn't seem quite definitive. (The alpha and beta angles are germane, but mention of them tends to confuse as much as to resolve the issue). In the simplest sense, the extended lattice arises because the Sec13/31 rods can assemble into an array of squares (rhomboids). The +/+ and -/- arrangement of rods is not absolutely required for a rhomboidal lattice to form; a +/- arrangement would work, it would retain two-fold vertex centers. Rather, the importance of the +/+ -/- arrangement is presumably that this geometry brings the Sec13/31 lattice into close (though still imperfect) register with the inner Sec23/24-Sar1 lattice. Regarding this point, it isn't clear how continuous the +/+ -/- arrangement really is along the tubular membranes. In the Table of Figure 3 - figure supplement 3, the difference between the cell edges for the left-handed (31.9 nm) and right-handed (30.2 nm) “helices” seems a bit small. It would be useful if the authors could measure this based on the Sec13/31 crystal structure. Could there in fact be occasional dislocations in the +/+ -/- lattice?

The reviewers suggest that the difference between the +/+ and -/- edges (17Å) seems a bit small. We have measured the expected difference based on the crystal structure fitted into the cuboctahedral cage, finding it to be approximately 40Å. This measurement does not consider that changes in the vertex angles or straightening of the rods may alter the difference in length. Nevertheless, the difference between the rod lengths is indeed smaller than expected. We have included a comparison with the crystal structure.

The fact that the difference is smaller than expected may, as the reviewers suggest, result from dislocations in the lattice (we are now explicit about this in the manuscript: “Gaps and dislocations in both inner and outer coat lattices may further contribute to curvature variability”). The reviewers are correct that a +/- arrangement could also assemble a rhomboidal lattice, but this would require firstly that the vertex can deform to the point that alpha and beta angles are equal and secondly that it can be oriented in two ninety-degree rotated positions relative to the underlying curvature of the tube surface. In contrast, the +/+, -/- arrangement allows a rhomboidal lattice to form while maintaining a smaller alpha angle and larger beta angle at all vertices. Put more simply, assembling a rhomboidal lattice on the surface of the tube using a +/- arrangement would require a more substantial deformation of the vertex. In the original submission, the supplement contained a section on the assessment of the outer coat arrangement, where the observations indicating a predominantly “+/+” and “-/-” arrangement are described. We have edited this section (see the quote above). Further, we have clarified this issue in the main text.

We have also included an extra panel in Figure 3–figure supplement 2, with the legend:

“A rhomboidal lattice could take two forms. In the first, each vertex would have the same orientation relative to the tube (left-hand panel)…”

2) Tubular variants of round structures were among the earliest coat structures ever examined by electron microscopy - the polyheads of T4 and related bacteriophages, studied in detail by Kellenberger and Klug in the '60s. Those groups examined different classes of polyheads and suggested how subunit packing might generate them. Recall also that T-even phages actually have elongated heads (because of a “waist” between the two rounded ends, each with hemi-icosahedral symmetry), and giant T4 phages have very long heads (extended tubular waists). More recently, the beautiful work of Sundquist and Finch on cone-like assemblies of the HIV-1 capsid protein illustrates an even more striking polymorphism that generates tapered tubes. So the unwarranted use at the end of the first paragraph in “Results and Discussion” of the word “unexpectedly” (an interjection that should be used sparingly, if at all, in scientific publications) is either an admission of ignorance or an unnecessary attempt to impart “novelty”. If procollagen and other elongated molecules are transported by the COPII pathway, then tubular structures with rounded ends are precisely what we can expect. The structural results presented here are interesting, not because they show tubes with hemispherical ends, but rather because the structure of the tubes is not the only one that could have been imagined from the lattice of isometric coats and because the lattice has a cogent interpretation in terms of the high resolution structures of its components. The authors should add some discussion of the clear and relevant precedents for their results.

We had written: “Unexpectedly, the inner coat layer also appeared as a regular array”. To us this was indeed unexpected, but we understand that the word is ill advised and have removed it.

We thank the reviewers for suggesting a comparison of how the structural flexibility of the COPII lattice relates to previous work on elongated phage heads and HIV CA arrays. We have, as requested, added a discussion of these systems to the manuscript.

3) The authors should pay attention to the notion of quasi-equivalence and the way in which high-resolution structures of icosahedral viruses have given this notion specific instantiations and modified earlier formulations. The authors appear not to appreciate that there is important precedent in work on virus structures, especially Klug's classic studies that followed in the initial work on polyheads. The different conformations for the edges (hinge bending) are quite consistent with similar variations in virus capsid assemblies and polymorphisms. In this respect, the description of edges as “++”, “+-”, etc. is confusing. Designations based on the contacts at the ends, rather than on the conformation of the edge dimer itself, may mislead the reader into thinking that there is some inherent asymmetry in the dimer, rather than an asymmetry in its bonding (which may or may not generate some longer-range asymmetry in its conformation).

We have added a comparison to virus structures (see response to the previous point.)

We appreciate the risk that the reader may be confused by the ambiguities between asymmetry in bonding and inherent asymmetry in the Sec13/31 dimers. We believe it is appropriate to adopt the same “+” and “-” notation used in the previous literature for describing the contacts at the ends of the rods, and believe that the succinct “+/-” notation for describing rods is preferable to introducing longer phrases such as “rods making a + contact at one end and a – contact at the other end”. To minimize the risk of confusion we have edited the text in two key positions:

A) “Within the in vitro assembled cages each Sec13/31 rod makes a “+” contact at one end and a “-” contact at the other end.”

B) “Together these data imply that three properties contribute to outer coat adaptability…”

4) The model at the end impresses two of the reviewers as unlikely or unnecessary. The packaging of an elongated cargo can drive the switch from hemispherical to tubular, just as some variation of membrane tension or other stochastic fluctuation has presumably driven the switch during the in vitro assembly of the coats studied here. Moreover, there is no reason why the outer-layer and inner-layer proteins cannot co-assemble, with the lattice of the latter imposing regularity on the packing of the former. Positing regulatory proteins that somehow communicate with cargo could send cell biologists on unnecessary wild-goose chases. In the case of elongated phage heads, an internal protein (the scaffold) appears to dicate the formation of a tubular part: it is really akin to cargo in the case of a vesicle. (The phage DNA gets stuffed in later, after the scaffold goes away.) Note that an example of a coat protein that appears not to form tubular structures (i.e., lattices curved in one direction but not the other, instead of lattices with two dimensions of curvature) is clathrin, probably because it is bent at a trimeric vertex, which can flatten (more or less) but not in one direction only. In that case, elongated cargos cause coat assembly to stall, because the shell curves back against the membrane that surrounds the cargo and cannot continue; recruitment of actin then drives further engulfment.

We agree with the reviewers that cargo packaging could drive the switch from spherical to tubular morphology, and that the inner and outer coats could co-assemble, with the inner coat imposing regularity on the outer coat, or vice versa. We regret a misunderstanding: the purpose of our model was not to posit regulatory proteins that communicate with cargo. This suggestion is in fact already prevalent in the COPII literature, and candidate proteins have already been identified. For example “… specific proteins may control the loading of big cargoes and regulate the size of COPII carriers accordingly… Recent findings have revealed that the proteins TANGO1 and cTAGE5 assemble into a dimer at the ER exit sites and that both are required for Collagen VII secretion. TANGO1 binds collagen VII and both TANGO and cTAGE5 bind COPII proteins Sec23/24… TANGO1 and its binding partners are therefore excellent candidates for factors that could delay Sar1-GTP hydrolysis in a cargo-dependent manner, by binding Sec23/Sec24 and delaying recruitment of the Sec13/Sec31 complex. In this manner, TANGOs can promote extended growth of COPII carriers.” (Malhotra and Erlmann, EMBO J., 2011) and “…there has been important progress in identifying receptors such as TANGO1 that guide the packing of PCs into COPII transport carriers… In our working hypothesis, the combined action of TANGO1 and Sedlin coordinates the stabilization of the inner COPII layer (through TANGO1) with efficient Sar1 cycling (through Sedlin) to prevent premature membrane constriction, thus allowing growth of nascent carriers and the incorporation of large PC prefibrils…” (Venditti et al., Science, 2012). Our model was intended to place our data in the context of this existing literature. Given that our data is relevant to an extensive existing discussion of carrier size modulation, we think it is appropriate to maintain some discussion of this point in the paper. Considering the concerns of the reviewers, we have edited the final paragraph of the discussion as well as the legend to Figure 7B.

We hope that this new formulation of the model is a useful contribution, and does not risk sending anyone on a wild-goose chase.

5) Finally, there are some technical aspects of the cryo-electron tomography that the authors should address. The information provided on the subvolume averaging is minimal, so it is not possible to assess the quality of the data. A few representative tomograms of the actual data would help. Further, the use of CTF correction is not well supported. The authors need to show power spectra without CTF correction to show that there was measurable signal beyond the first zero (for data in Figure 4). At 200 kV, and at about 2 micron defocus, the first zero crossing of the CTF is about 22.5 Å, so the claim of resolution improvement with CTF correction is hard to understand. The actual resolution is probably around 30 Å, notwithstanding the FSC plots, which are especially unreliable for defining resolution in sub-tomogram averages. Nevertheless, the mechanistic conclusions could be drawn even if the data were only at resolutions of 30-40 Å.

All structures, all fits, and a representative tomogram from the raw data have been deposited in the EMDB/PDB.

The range of defocuses described in the data processing section for the close-to-focus data (1.5-2.5 μm) referred to the nominal defocus at data collection, while the measured defocus of the dataset ranged from 2.0-3.2 µm. We apologize for the ambiguity, which has now been corrected.

This places the first node of the mean CTF of the data at approximately 27 Å, meaning that CTF correction is required to obtain a resolution of 20 Å.

We have now added a section to the Methods and a supplementary figure containing a validation of the importance and success of the CTF correction: we took the final reconstruction of the inner coat from CTF corrected and CTF uncorrected data, and compared it to a volume generated from the arrayed crystal structure of the Sec23/24/Sar1 complex. The FSCs between the X-ray structure and the corrected EM structure show positive correlation to a resolution of ∼20 Å, whereas the FSC between the X-ray structure and the uncorrected EM structure shows a phase inversion at approximately 27 Å as expected. This is included in Figure 4–figure supplement 1, which also includes a visualization of the map in comparison with the arrayed crystal structures filtered to the resolution of the reconstruction by multiplication with the FSC.

We note that the resolution of the map has been measured using a “gold standard” approach (the two half datasets have been processed completely independently at all stages, and a low-pass filter was applied at 45 Å during alignment). Further, the mask used for resolution measurement (which can indeed promote an over-estimate of resolution) was cautious: a sphere with a five-pixel Gaussian fall off. We have clarified this in the text.

We have added text to clarify the defocus measurement approach, where we did not make use of the image power spectra.

While we agree with the reviewer that the same conclusions could be drawn had we obtained a lower-resolution structure, we should nevertheless include our best estimate of the correct resolution. For the reasons presented above we have confidence in both the CTF correction and the resolution estimate. Within the revised main text we now use the 0.5 criterion value of 23 Å.

Author details

1. Giulia Zanetti

   Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States

   Present address

   Department of Biological Sciences, Institute of Structural and Molecular Biology, London, United Kingdom

   Contribution

   GZ, Analysis and interpretation of data, Drafting or revising the article

   Competing interests

   No competing interests declared.

2. Simone Prinz

   Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany

   Contribution

   SP, Performed and optimized in vitro reconstitution, Acquisition of data

   Competing interests

   No competing interests declared.

3. Sebastian Daum

   HALOmem, Martin Luther University of Halle-Wittenberg, Halle, Germany

   Contribution

   SD, Performed and optimized protein expression and purification

   Competing interests

   No competing interests declared.

4. Annette Meister

   HALOmem, Martin Luther University of Halle-Wittenberg, Halle, Germany

   Contribution

   AM, Performed and optimized in vitro reconstitution

   Competing interests

   No competing interests declared.

5. Randy Schekman

   1. Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States
   2. Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, United States

   Contribution

   RS, Conception and design

   Competing interests

   RS: Editor-in-Chief, eLife.

6. Kirsten Bacia

   HALOmem, Martin Luther University of Halle-Wittenberg, Halle, Germany

   Contribution

   KB, Conception and design

   Competing interests

   No competing interests declared.

7. John AG Briggs

   Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany

   Contribution

   JAGB, Conception and design, Analysis and interpretation of data, Drafting or revising the article

   For correspondence

   john.briggs@embl.de

   Competing interests

   No competing interests declared.

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