1. Plant Biology

Brassinosteroids control root epidermal cell fate via direct regulation of a MYB-bHLH-WD40 complex by GSK3-like kinases

  1. Yinwei Cheng
  2. Wenjiao Zhu
  3. Yuxiao Chen
  4. Shinsaku Ito
  5. Tadao Asami
  6. Xuelu Wang  Is a corresponding author
  1. Fudan University, China
  2. The University of Tokyo, Japan
  3. Huazhong Agricultural University, China
https://doi.org/10.7554/eLife.02525
Share this article
Cite this article
  1. Yinwei Cheng
  2. Wenjiao Zhu
  3. Yuxiao Chen
  4. Shinsaku Ito
  5. Tadao Asami
  6. Xuelu Wang
(2014)
Brassinosteroids control root epidermal cell fate via direct regulation of a MYB-bHLH-WD40 complex by GSK3-like kinases
eLife 3:e02525.
https://doi.org/10.7554/eLife.02525
  2. Download BibTeX
  3. Download .RIS

Abstract

In Arabidopsis, root hair and non-hair cell fates are determined by a MYB-bHLH-WD40 transcriptional complex and are regulated by many internal and environmental cues. Brassinosteroids play important roles in regulating root hair specification by unknown mechanisms. Here, we systematically examined root hair phenotypes in brassinosteroid-related mutants, and found that brassinosteroid signaling inhibits root hair formation through GSK3-like kinases or upstream components. We found that with enhanced brassinosteroid signaling, GL2, a cell fate marker for non-hair cells, is ectopically expressed in hair cells, while its expression in non-hair cells is suppressed when brassinosteroid signaling is reduced. Genetic analysis demonstrated that brassinosteroid-regulated root epidermal cell patterning is dependent on the WER-GL3/EGL3-TTG1 transcriptional complex. One of the GSK3-like kinases, BIN2, interacted with and phosphorylated EGL3, and EGL3s mutated at phosphorylation sites were retained in hair cell nuclei. BIN2 phosphorylated TTG1 to inhibit the activity of the WER-GL3/EGL3-TTG1 complex. Thus, our study provides insights into the mechanism of brassinosteroid regulation of root hair patterning.

https://doi.org/10.7554/eLife.02525.001

eLife digest

Roots anchor a plant into the ground, and allow the plant to absorb water and mineral nutrients from the soil. As roots grow and branch, they increase the surface area of root exposed to the soil—and many plant cells in the root's outer layer have a hair-like projection to further increase this surface area. Thus, root hairs are where most water and mineral nutrients are absorbed. Many factors affect whether, or not, a plant cell will develop into a root hair. These factors include both external cues (such as the mineral content of the soil) and signals from the plant itself (such as hormones).

Brassinosteroids are plant hormones that regulate the development of shoots and roots, as well as the timing of when flowers begin to develop. These hormones are detected on the outside of plant cells, and activate a signaling pathway within the cell that causes changes in gene expression. Brassinosteroids also control if a root cell will become a hair cell or not, although the mechanism behind this activity is unclear.

Here, Cheng et al. have looked at the root hairs of mutant Arabidopsis thaliana plants that have had individual genes involved in brassinosteroid signaling knocked-out. Plant biologists commonly study this plant species because it is small and grows quickly—and Arabidopsis has regular stripes of root hair cells and ‘non-hair cells’ in the outer layer of its roots. Cheng et al. reveal that brassinosteroids prevent the formation of root hairs via signaling pathways that involve proteins called GSK3-like kinases. These hormones ‘switch off’ these kinases’ activity, so knocking-out the genes that code for these kinases has the same effect as adding extra brassinosteroids to the plant roots: fewer root hair cells.

Cheng et al. show that one of the GSK3-like kinases binds and adds phosphate groups to protein complexes that control gene expression—and this causes these protein complexes to be less active. When GSK3-like kinase activity is switched off by brassinosteroids, these complexes instead become more active and trigger the expression of genes that direct a plant cell to become a non-hair cell.

The findings of Cheng et al. reveal the pathways that allow brassinosteroids to stop plant cells in roots from becoming hair cells, and that instead encourage these cells to become non-hair cells. However, further work is needed to uncover how the striped pattern of hair cells and non-hair cells on Arabidopsis roots is established, and how brassinosteroids work with other plant hormones to control this pattern.

https://doi.org/10.7554/eLife.02525.002

Introduction

The Arabidopsis root epidermal cell types are defined by position in a predictable manner (Ishida et al., 2008). Hair (H) cells, or trichoblasts, are specified early from root epidermal cells that lie over clefts between two underlying cortical cells, whereas the root epidermal cells that lie over a single cortical cell develop as non-hair (N) cells, or atrichoblasts (Ishida et al., 2008). Hair cell and non-hair cell files are patterned alternately in rows within the Arabidopsis root epidermis, with columns of hair cells interspersed with columns of non-hair cells (Schiefelbein et al., 2009). Prior to root hair outgrowth, root epidermal cells in the H position can be distinguished from those in the N position by many visible cellular features, including a greater rate of cell division (Berger et al., 1998), reduced cell length and vacuolation (Dolan et al., 1994; Galway et al., 1994), and enhanced cytoplasmic density (Dolan et al., 1994). It is proposed that positional signals and a putative receptor-like kinase SCRAMBLED (SCM) (Kwak et al., 2005) function through a MYB-bHLH-WD40 repeat transcriptional complex to determine root epidermal cell fate (Schiefelbein et al., 2009). Based on this model, in N cells, WEREWOLF (WER) (Lee and Schiefelbein, 1999), a R2R3 MYB-domain transcription factor, forms a complex with basic helix-loop-helix transcription factors, GLABRA3 (GL3)/ENHANCER OF GLABRA3 (EGL3) (Bernhardt et al., 2003; Zhang et al., 2003), and a WD40 repeat protein, TRANSPARENT TESTA GLABRA1 (TTG1) (Galway et al., 1994), to promote expression of GLABRA2 (GL2) and CAPRICE (CPC) (Ryu et al., 2005; Song et al., 2011). GL2, a homeodomain/leucine zipper transcription factor, negatively regulates H cell fate and positively regulates N cell fate (Masucci et al., 1996). CPC (Wada et al., 1997), a MYB-type transcription factor, moves from N cells to H cells (Kurata et al., 2005a) to compete with WER for binding to GL3/EGL3 to form a CPC-GL3/EGL3-TTG1 complex, which is unable to induce GL2 expression (Song et al., 2011). In addition to CPC, the bHLH transcription factor GL3 is also a mobile protein (Bernhardt et al., 2005). GL3 and its homologue EGL3 are both expressed in H cells, but GL3 protein is only localized in the N cell nucleus, indicating that GL3 protein moves into the adjoining N cell nucleus to determine N cell fate (Bernhardt et al., 2003, 2005). Integration of existing genetic and biochemical data also supports an alternative mechanism centered on the movement of transcriptional factors between epidermal cells rather than a putative local activation of the WER gene function to determine root epidermis pattern formation (Savage et al., 2008).

In addition, root hair development is highly regulated by many external and internal cues, including phytohormones. For instance, abscisic acid (ABA) plays a role in the early stage of root epidermal cell specification (Van Hengel et al., 2004) and in inhibiting root hair tip growth in Arabidopsis (Schnall and Quatrano, 1992), while both ethylene and auxin may act downstream of TTG1 and GL2 to promote root hair formation and elongation (Masucci and Schiefelbein, 1994, 1996). Moreover, jasmonic acids (JAs) promote root hair formation through their interaction with ethylene (Zhu et al., 2006). However, the underlying cellular and molecular mechanisms of how these internal hormones integrate with environmental cues to regulate root hair cell fate determination are still poorly understood.

The plant steroid hormones, brassinosteroids (BRs), play essential roles in regulating many developmental processes, including shoot, root, and reproductive development (Savaldi-Goldstein et al., 2007; Ye et al., 2010; Hacham et al., 2011; Yang et al., 2011). BRs are perceived by the receptor kinase BRASSINOSTEROID INSENSITIVE 1 (BRI1) (Li and Chory, 1997; Hothorn et al., 2011; She et al., 2011). The BR-activated BRI1 phosphorylates BRI1 KINASE INHIBITOR 1 (BKI1) to release its inhibition (Wang and Chory, 2006), and then BKI1 acts as a positive regulator by binding to a subset of 14-3-3 proteins (Wang et al., 2011). Another BRI1 substrate, BR-SIGNALING KINASE (BSK), transduces the BR signaling through bri1 SUPPRESSORS 1 (BSU1) to inactivate a GSK3-like kinase BRASSINOSTEROID INSENSITIVE 2 (BIN2), which leads to accumulation of the dephosphorylated form of transcriptional factors BRI1 EMS SUPPRESSOR 1 (BES1)/BRASSINAZOLE RESISTANT 1 (BZR1) in the nucleus to regulate gene expression (Yang et al., 2011). A previous study suggests that BRs play an important role in determining root epidermal cell fate by regulating WER and GL2 expression (Kuppusamy et al., 2009). However, the elaborate molecular mechanism by which BRs regulate root epidermal cell fate and development is still unknown.

Here, we first systematically examined root epidermal cell patterning and PGL2::GUS expression in a series of BR-deficient and signaling mutants. We found that BRs regulate root epidermal cell fate through promoting GL2 expression in both H and N cells, which is mediated by GSK3-like kinases and the WER-GL3/EGL3-TTG1 complex as indicated by genetic analysis and biochemical studies. Our study further demonstrated that BIN2, one of the GSK3-like kinases, interacted with and phosphorylated EGL3 on T399 and T209/T213, leading to its trafficking from nucleus to cytosol in H cells, which may facilitate its movement from H cells to N cells. BIN2 also phosphorylated TTG1 to inhibit the activity of the WER-GL3/EGL3-TTG1 transcriptional complex. These results explain how BR signaling regulates both the formation and activity of the WER-GL3/EGL3-TTG1 complex through GSK3-like kinases to coordinate root epidermal cell fate specification.

Results

BRs regulate root epidermal cell patterning through GSK3-like kinases

To broadly explore the role of BRs in root hair formation, we systematically examined the root hair phenotype of BR-biosynthetic mutants, det2-1 and cpd, and BR-responsive mutants, including bri1-116, BRI1-OX (a BRI1-overexpression line), bin2-3 bil1 bil2 (a triple knockout mutant of BIN2 and its two closest homologues), and a BES1-RNAi line. We found that the relative hair number (=root hair density × root hair cell length) was higher in bri1-116 (4.67 ± 0.47), det2-1 (4.75 ± 0.52), and cpd (4.65 ± 0.54), and significantly lower in BRI1-OX (3.40 ± 0.46) and bin2-3 bil1 bil2 (2.86 ± 0.39) than in their corresponding wild types Col-0 (3.89 ± 0.43) and WS-2 (3.85 ± 0.41) (Figure 1—source data 1). However, there was no significant difference between BES1-RNAi and Col-0. Images at the highest magnification (×100; Figure 1) showed that in the wild type root (Figure 1A), the H cells and N cells were arranged in alternating files with the H cell columns regularly interspaced with the N cell columns; no adjacent H cell columns were found. However, in the BR signaling-inhibited mutants, including bri1-116, det2-1, and cpd (Figure 1B–D), many root hair columns were next to each other, leading to more root hairs, suggesting that some N cell fate might be changed into H cell fate. In contrast, the BR signaling-enhanced plants, including BRI1-OX and bin2-3 bil1 bil2, grew fewer root hairs than the wild type, due to the fact that they lacked root hairs in many H cell positions (Figure 1A,E–G). Interestingly, the BES1-RNAi line showed a similar root hair pattern as the wild type (Figure 1A,H).

Figure 1 with 1 supplement see all
Download asset Open asset
Root epidermal cell patterning is altered in the BR-related mutants.

(A–H) Root hair patterning of the BR-related mutants and their wild type counterparts. bin2-3 bil1 bil2 is in the WS-2 background, and all of the others are in the Col-0 background. (I–L) Root hair phenotype of the wild type plants grown on 1/2 MS (Murashige and Skoog) medium with DMSO (mock) (I), 100 nM epibrassinolide (eBL) (J), 30 μM bikinin (Bik) (K), or 1 μM brassinazole (Brz) (L). Right images are the outlined areas of left images with higher magnification. Arrows indicate ectopic root hair cells, and arrowheads indicate ectopic non-hair cells. Areas outlined with blue lines indicate the ectopic non-hair cells.

https://doi.org/10.7554/eLife.02525.003
Figure 1—source data 1

Root hair density, cell length, and relative hair number of the BR-related mutants and wild type plants treated with eBL, bikinin, Brz, or DMSO

https://doi.org/10.7554/eLife.02525.004

To further test whether exogenously applied eBL (epibrassinolide), bikinin (a specific GSK3 kinase inhibitor) (De Rybel et al., 2009), or Brz (brassinazole, an inhibitor of BR biosynthesis) regulate root hair specification, we planted seeds on 1/2 MS (Murashige and Skoog) medium containing each of these chemicals or DMSO (as the mock treatment), and carefully observed their root hair phenotypes. We found that compared with plants grown on the mock medium (3.80 ± 0.45), the relative root hair number of plants grown on medium containing 100 nM eBL (3.22 ± 0.42) or 30 μM bikinin (2.71 ± 0.50) was significantly reduced, while it was greatly increased in plants grown on medium containing 1 μM Brz (4.17 ± 0.43) (Figure 1—source data 1). Images at higher magnification (×100) show that, compared to seedlings grown on the mock medium (Figure 1I), those grown on medium containing eBL or bikinin produced fewer root hairs in the H position of epidermal cells (Figure 1J,K), while those grown on medium containing Brz grew more root hairs in the N position (Figure 1L). We also found that the bin2-3 bil1 bil2 seedlings grown on medium containing 30 μM bikinin produced very few root hairs (Figure 1—figure supplement 1), suggesting that besides BIN2, BIL1, and BIL2, other GSK3-like kinases may also be involved in root hair specification in Arabidopsis. Taken together, these findings indicate that the BR-mediated root epidermal cell pattern formation largely relies on GSK3-like kinases and/or their upstream components.

BR signaling promotes N cell fate and inhibits H cell fate

GL2 has been widely used as a molecular maker of N cell fate determination (Masucci et al., 1996; Kuppusamy et al., 2009). In order to test whether the disordered root hair patterning in the BR-related mutants resulted from an altered root epidermal cell fate, we analyzed the GL2 expression pattern in these mutants using PGL2::GUS as a reporter. We found that in the wild type, 1.3% of epidermal cells in the N position lacked GL2 expression in cross-sections (Figure 2A,B), and in the longitudinal view of root epidermis, the root epidermal cell files were arranged regularly, with GL2-expressing columns (N cell columns) interspaced with columns without GL2 expression (H cell columns) (Figure 2C). However, about 15.3% of bri1-116 and 18.8% of det2-1 cells showed suppressed PGL2::GUS expression, and there were adjacent root epidermal cells without GL2 expression in both bri1-116 and det2-1 (Figure 2D–I), which supports a previous finding with bri1-116 and the Brz-treated wild type (Kuppusamy et al., 2009). These results indicated that adjacent root hairs in bri1-116 and det2-1 were caused by some N cell fate changing to H cell fate. In contrast, GL2 was ectopically expressed in about 13.5% of H cells in BRI1-OX plants (Figure 2J–L) and in about 19.6% in bin2-3 bil1 bil2 (Figure 2M–O), as compared with only 3.2% in the wild type (Figure 2A–C), indicating that lack of GL2 expression in N cells may correspond to the ectopic root hairs observed in bri1-116 and det2-1, and that the ectopically expressed GL2 in H cells partially inhibits H cell fate in BRI1-OX and bin2-3 bil1 bil2 plants. Taken together, the above results suggested that BR signaling has an important role in suppressing H cell fate and promoting N cell fate in both the N and the H positions, and BR signaling regulates root epidermal cell fate by controlling GL2 expression through GSK3-like kinases, or their upstream components, but not through downstream transcription factors.

Figure 2
Download asset Open asset
Expression pattern of PGL2::GUS is altered in the BR-related mutants.

Transverse sections from root meristem of Col-0 (A), bri1-116 (D), det2-1 (G), BRI1-OX (J), and bin2-3 bil1 bil2 (M). Frequency of cells without PGL2::GUS expression in the N cell position (open bars) and cells with ectopically expressed PGL2::GUS in the H cell position (solid bars) of Col-0 (B), bri1-116 (E), det2-1 (H), BRI1-OX (K), and bin2-3 bil1 bil2 (N). Longitudinal images of the root epidermal cells in Col-0 (C), bri1-116 (F), det2-1 (I), BRI1-OX (L), and bin2-3 bil1 bil2 (O). Scale bars, 25 μm. Red arrows indicate N cells without PGL2::GUS expression, and red arrowheads indicate H cells ectopically expressing PGL2::GUS. For each genotype, n = 8. Error bars indicate standard deviation (SD).

https://doi.org/10.7554/eLife.02525.006

BR signaling acts upstream of WER and CPC to regulate root epidermal cell fate

It was known that GL2 expression is directly regulated by the WER-GL3/EGL3-TTG1 but not the CPC-GL3/EGL3-TTG1 transcriptional complex (Schiefelbein et al., 2009). To explore whether the BR-regulated GL2 expression and root epidermal cell fate determination are dependent on these complexes, we first created a set of double mutants of cpc-1, a mutant with fewer root hairs than its counterpart (Figure 3A,B), with bri1-116 or cpd (Figure 3C,D). Similar to cpc-1, both double mutants bri1-116 cpc-1 (Figure 3E) and cpd cpc-1 (Figure 3F) produced few root hairs. We also generated double or multiple mutants of wer-1, a mutant with more root hairs than Col-0 (Figure 3G,H), with BRI1-OX or bin2-3 bil1 bil2 (Figure 3I,J), and found that both BRI1-OX wer-1 and bin2-3 bil1 bil2 wer-1 (Figure 3K,L) were similar to wer-1, with many ectopic root hairs formed at the N cell position. These genetic analyses indicated that the WER-GL3/EGL3-TTG1 and CPC-GL3/EGL3-TTG1 transcriptional complexes act downstream of BR early signaling.

Figure 3
Download asset Open asset
BR signaling acts upstream of CPC and WER to regulate root epidermal cell fate.

Root hair phenotype of the wild type WS-2 (A) and double mutants of cpc-1 (B) with bri1-116 (C) or cpd (D), including bri1-116 cpc-1 (E) and cpd cpc-1 (F). Root hair phenotype of the wild type Col-0 (G) and the double/multiple mutants of wer-1 (H) with BRI1-OX (I) or bin2-3 bil1 bil2 (J), including BRI1-OX wer-1 (K) and bin2-3 bil1 bil2 wer-1 (L). cpc-1 is in the WS-2 background. BR: brassinosteroid.

https://doi.org/10.7554/eLife.02525.007

BIN2 phosphorylates EGL3 and TTG1

Therefore, we inferred that BR-mediated root epidermal cell fate may be dependent on GSK3-like kinases, the key negative components in the BR signaling pathway, acting upstream of the WER-EGL3/GL3-TTG1 or CPC-EGL3/GL3-TTG1 transcriptional complex. We then conducted yeast two-hybrid assays to test whether any components in the WER-EGL3/GL3-TTG1 or CPC-EGL3/GL3-TTG1 complex interact with BIN2, a well-studied GSK3-like kinase, and found that BIN2 can interact with EGL3 (Figure 4A) but not with CPC in yeast (Figure 4—figure supplement 1). However, due to strong auto-activation of WER and TTG1 fused with GAL4-DNA binding domain (DB) in yeast two-hybrid assays (Figure 4—figure supplement 1), we conducted GST pull-down and BiFC (biomolecular fluorescence complementation) assays to test their interactions, and found that BIN2 can interact with WER, EGL3, and TTG1 (Figure 4B,C). Furthermore, because BIN2 can regulate many transcription factors by phosphorylation (Saidi et al., 2012), and WER or CPC can interact with EGL3/GL3-TTG1 in vivo to form WER-EGL3/GL3-TTG1 or CPC-EGL3/GL3-TTG1 complexes, respectively (Zhao et al., 2008; Song et al., 2011), we then conducted in vitro kinase assays to test whether BIN2 can phosphorylate any of these components. We found that BIN2 did not phosphorylate WER and CPC, but was able to phosphorylate EGL3 and TTG1 (Figure 5).

Figure 4 with 1 supplement see all
Download asset Open asset
BIN2 interacts with EGL3, TTG1, and WER.

(A) BIN2 interacts with EGL3 in yeast two-hybrid assays. (B) The interaction of BIN2-His with CPC-GST, EGL3-GST, TTG1-GST, and WER-GST in vitro. The BIN2-His pulled-down by CPC-GST, EGL3-GST, TTG1-GST, and WER-GST, or GST was detected by western blotting with anti-His antibody (top). The purified BIN2-His protein was used as inputs. An equal loading of recombinant proteins was indicated by Coomassie brilliant blue (CBB) staining (bottom). (C) BiFC assays of the interaction between BIN2 with EGL3, TTG1, and WER. Scale bars, 20 μm.

https://doi.org/10.7554/eLife.02525.008
Figure 5 with 1 supplement see all
Download asset Open asset
BIN2 phosphorylates EGL3 and TTG1, but not WER and CPC in vitro.

An equal amount of recombinant BIN2 kinase indicated by Coomassie brilliant blue (CBB) staining (bottom) was incubated with recombinant MBP, WER-MBP, CPC-MBP, EGL3-MBP, or TTG1-MBP, separated by SDS–PAGE, and followed by autoradiography (top).

https://doi.org/10.7554/eLife.02525.010

GSK3-like kinase phosphorylates EGL3 to promote its movement to N cells

To investigate the biological relevance of EGL3 phosphorylation by a GSK3-like kinase BIN2, we used mass spectrometry and identified four potential phosphorylation sites (T209, T213, T399, and T403) of EGL3 by BIN2 (Figure 5—figure supplement 1), which are located in two regions that contain typical recognition sites of GSK3 kinases (Cohen and Frame, 2001). We then mutated threonine residues into alanine to make single- or double-site mutated forms of EGL3. In vitro phosphorylation assays showed that phosphorylation levels of EGL3T399A and EGL3T209A/T213A by BIN2 were significantly reduced (Figure 6A), indicating that T399 and T209/T213 are the main phosphorylated residues. To investigate the biological function of EGL3 phosphorylation, we transformed EGL3 and its mutated forms driven by its own promoter into Col-0 to examine their subcellular localization. We found that the EGL3-GFP signal in N cells was apparently higher than that in H cells, and that it was mainly localized to cytosol in H cells, but to both cytosol and nucleus in N cells (Figure 6B). As EGL3 mRNA is only expressed in H cells (Bernhardt et al., 2005), this result indicated that, like its homologue GL3, EGL3 is also a mobile protein that moves from H cells to N cells. However, the EGL3T399A-GFP and EGL3T209A/T213A-GFP were solely localized to the nuclei of H cells (Figure 6C,D), indicating that EGL3 phosphorylation is required not only for its cytoplasmic accumulation, but also for its movement from H cells to N cells. In addition, we found that the root hair patterning of EGL3-GFP transgenic plants was not altered (Figure 6E; Table 1), indicating that correctly localized EGL3, mainly in N cell nuclei to promote N cell fate and less in H cell nuclei not to promote N cell fate, has no influence on root epidermal fate. Moreover, although root epidermal patterning in the EGL3T399A-GFP transgenic plants was normal (Figure 6F; Table 1), the number of root hairs in EGL3T209A/T213A-GFP plants was significantly reduced, likely due to a misspecification of non-hair cells in the H position (Figure 6G; Table 1), suggesting that the nucleus-localized EGL3 in H cells may determine N cell fate specification. Taken together, our results indicate that BIN2 phosphorylation on T399, T209, and/or T213 of EGL3 in H cells promotes EGL3 cytoplasmic localization, which likely helps its movement from H to N cells to regulate root epidermal cell fate.

Figure 6 with 1 supplement see all
Download asset Open asset
BIN2 phosphorylates EGL3 to regulate its subcellular localization and root epidermal cell fate.

(A) BIN2 phosphorylates EGL3 on T399 and T209/T213. An equal amount of recombinant protein, as indicated by Coomassie brilliant blue (CBB) staining (bottom panel), was incubated in phosphorylation buffer, separated by SDS–PAGE, and followed by autoradiography (top panel). (B) EGL3-GFP is predominantly localized in N cell nuclei. Both EGL3T399A-GFP (C) and EGL3T209A/T213A-GFP (D) are solely localized in H cell nuclei. For (B–D), the 5-day-old roots were stained with propidium iodide (red) for 10 s for visualizing the cell wall. The top panels show the underlying cortex. The stars indicate H cells. Scale bars, 20 μm. (E–G) Root hair patterns of EGL3-GFP (E), EGL3T399A-GFP (F), and EGL3T209A/T213A-GFP (G) transgenic plants. Outlined areas in the left images are magnified in the right images. Red arrowheads and areas outlined with blue lines indicate ectopic non-root hair cells in the H position.

https://doi.org/10.7554/eLife.02525.012
Figure 6—source data 1

EGL3 amino acid sequence analysis

https://doi.org/10.7554/eLife.02525.013
Table 1

The effect of EGL3 and its two mutated forms on root epidermal cell pattern formation

https://doi.org/10.7554/eLife.02525.015
GenotypeCells in the H positionCells in the N position
Hair cells (%)Non-hair cells (%)Hair cells (%)Non-hair cells (%)
Col-098.9 ± 3.31.1 ± 3.32.0 ± 4.598.0 ± 4.5
PEGL3::EGL3-GFP95.8 ± 6.14.2 ± 6.11.9 ± 4.298.1 ± 4.2
PEGL3::EGL3T399A-GFP93.1 ± 6.46.9 ± 6.42.8 ± 6.097.2 ± 6.0
PEGL3::EGL3T209A/T213A-GFP85.5 ± 4.4*14.5 ± 4.4*1.0 ± 3.299.0 ± 3.2

  1. At least 10 different 5-day-old roots were examined for each strain. Values represent means ± SD. For statistical analysis, the F test was used to determine the variance, and the two-tailed t test with equal variance or unequal variance was used to determine the significance level of the difference among the transgenic plants.

  2. *

    p<0.05.

GSK3-like kinases phosphorylate TTG1 to suppress the transcriptional activity of the WER-EGL3-TTG1 complex

TTG1 is required for a normal expression level of GL2 (Di Cristina et al., 1996) but not for its expression pattern (Hung et al., 1998). Transgenic TTG1-GFP plants driven by its own promoter indicated that TTG1 was preferentially localized in the cytoplasm and slightly in the nucleus of both N and H cells (Figure 7—figure supplement 1), which was consistent with the subcellular localization of its petunia homologue AN11 (De Vetten et al., 1997). To understand the biological relevance of TTG1 phosphorylation by a GSK3-like kinase, we conducted transient transcription assays in Nicotiana benthamiana leaves to examine whether BIN2 regulated the complex's activity in a TTG1-dependent manner. We constructed a dual-luciferase reporter system using PGL2::LUC as a reporter gene and 35S::REN as an internal control (Figure 7A). Because the protein of the gain-of-function mutation bin2-1 (E263K) is more stable and has higher activity than the wild type BIN2 (Peng et al., 2008), and GSK3-like kinases are quite conserved among different species (Saidi et al., 2012), we used bin2-1 to conduct this study. As shown in Figure 7B, transient expression of WER alone was able to slightly induce PGL2::LUC gene expression. In contrast, transient expression of EGL3 alone was unable to induce reporter gene expression. Co-expression of WER and EGL3 can dramatically promote LUC expression, which is consistent with a previous study (Song et al., 2011). Additional bin2-1 did not alter the effect of WER, EGL3, or both WER and EGL3 on PGL2::LUC expression. When WER, EGL3, and TTG1 were used together, the expression of PGL2::LUC was further enhanced, indicating that TTG1 can promote the activity of the WER-EGL3 complex. Interestingly, additional bin2-1 significantly inhibited reporter gene expression regulated by the WER-EGL3-TTG1 complex (Figure 7B), indicating that TTG1 is mediating the negative effect of BIN2 on this transcriptional complex.

Figure 7 with 1 supplement see all
Download asset Open asset
BIN2 inhibits the transcription activity of the WER-EGL3-TTG1 complex through TTG1.

(A) Schematic diagram of the dual-luciferase reporter construct. The firefly luciferase (LUC) reporter gene was driven by GL2 promoter. The Renillia luciferase (REN) reporter gene was controlled by Cauliflower mosaic virus promoter (35S) and terminator (Ter). (B) bin2-1 inhibits PGL2::LUC expression only when TTG1 is co-expressed with WER and EGL3. Relative reporter activity in Nicotiana benthamiana leaf cells transiently transformed with the indicated effector, reporter, and regulatory constructs. G, W, E, and T indicate GL2, WER, EGL3, and TTG1, respectively. Error bars indicate SD. **p<0.01 determined by the two-tailed Student's t test.

https://doi.org/10.7554/eLife.02525.016

Discussion

BR signaling depends on GSK3-like kinases and the WER-EGL3-TTG1 complex to modulate root epidermal patterning

We provide several lines of evidence to strongly support an important role for BR signaling in directly regulating root hair cell fate. First, root hair patterning in the BR-biosynthetic and responsive mutants or in the wild type grown on eBL, bikinin, or BRZ, was dramatically altered, demonstrating that GSK3 kinases and/or their upstream components are mediating this regulation. Second, the expression pattern of the non-hair cell fate marker PGL2::GUS indicates that BR early signaling promotes N cell fate in the whole root epidermis, which is a reasonable explanation for the abnormal root hair patterning in the BR-related mutants: when BR signaling is enhanced, fewer root hairs are formed in the H position; when BR signaling is inhibited, more ectopic root hairs are produced in the N position. This finding supports the previous report that BRs positively regulate the expression of WER and GL2 (Kuppusamy et al., 2009). Third, genetic analysis revealed that major components of the WER-EGL3-TTG1 or CPC-EGL3-TTG1 complex act downstream of BR signaling-mediated root epidermis patterning. Finally, BIN2 phosphorylation on EGL3 and TTG1 suggested that GSK3-like kinases directly regulate EGL3 movement and the transcription activity of the WER-EGL3-TTG1 complex to mediate root hair development.

Phosphorylation of EGL3 regulates its intercellular movement and controls root epidermal cell fate

This study revealed a key mechanism in the regulation of intercellular communication of transcription factors by a hormonal signal to determine epidermal cell specification. Non-cell autonomous movement of some transcriptional factors is an important mechanism to regulate some developmental processes (Kurata et al., 2005b), and cytoplasmic localization of these mobile proteins may be required for their intercellular movement. For example, in maize shoot apical meristem, a mutation in its potential nuclear localization signal (NLS) of KNOTTED 1 abolished its intercellular movement (Vollbrecht et al., 1991; Lucas et al., 1995), and cytoplasmic localization of LEAFY (Schultz and Haughn, 1991), a transcriptional factor in floral identity, is also strongly correlated with its adjacent cell movement (Wu et al., 2003). Moreover, the movement of SHORT-ROOT, another mobile transcriptional factor in root radical patterning (Helariutta et al., 2000), was abolished when it was fused to a NLS, leading to its diminished cytoplasmic localization (Gallagher et al., 2004). However, it is largely unknown how the nuclear-cytoplasmic trafficking of transcription factors is regulated by internal cues to influence their intercellular movement. Although the intercellular movement of mobile factors in the WER/CPC-EGL3/GL3-TTG1 complex may determine root epidermis patterning (Savage et al., 2008), it is not clear how their movement is regulated by internal cues. Because EGL3 mRNA was expressed only in H cells, the major nuclear localization of EGL3 protein in N cells indicated that, like GL3 and CPC, EGL3 can also move from H to N cells. In addition, we found that EGL3T399A and EGL3T209A/T213A with abolished phosphorylation sites were solely localized in the nucleus of H cells, indicating that the unphosphorylated EGL3 may not move between cells. Although we do not know how T209/T213 phosphorylation regulated EGL3 subcellular localization, the T399 phosphorylation likely affected a NLS, because T399 is located in a predicted non-canonical NLS (Figure 6—source data 1), which is found in many other plant bHLHs (Galstyan et al., 2012). Although the EGL3T399A-GFP plants showed normal root hair patterning, this can be explained by the close proximity of T399 to its bHLH domain, which may have affected its interaction with TTG1 and MYB (Zhang et al., 2003), leading to the nucleus-localized EGL3T399A in H cells unable to induce GL2 expression. However, EGL3T209A/T213A-GFP may still interact with TTG1 and have DNA-binding activity, because T209/T213 was located in the N-terminal region far away from the bHLH domain (Figure 6—figure supplement 1), which led to GL2 expression in H cells and EGL3T209A/T213A-GFP plants growing fewer root hairs (Figure 6G; Table 1).

GSK3-like kinases inhibit GL2 expression through TTG1, which is required for the transcriptional activity of WER-EGL3-TTG1 complex

Besides the regulation of EGL3 nuclear-cytoplasmic trafficking, we also provided strong evidence to support GSK3-like kinases’ inhibition on the transcriptional activity of the WER-EGL3-TTG1 complex through TTG1. In Nicotiana benthamiana pavement cells, it was demonstrated that BIN2 has a negative role in WER-EGL3-TTG1 transcriptional activity, but has no effect on the activity of WER, EGL3, or both WER and EGL3. Although BIN2 phosphorylates EGL3, its failure to regulate WER-EGL3 transcriptional activity can be explained by a possible ubiquitous expression of WER, EGL3, and GSK3-like kinases in Nicotiana benthamiana leaves. Furthermore, it was reported that TTG1 interacts with EGL3 (Zhang et al., 2003), and TTG1 is necessary for the full functioning of other bHLH partners, such as GL3 and TRANSPARENT TESTA8 (Baudry et al., 2004; Zhao et al., 2008). Therefore, it is very likely that TTG1 phosphorylation by GSK3-like kinases may affect its regulation of EGL3 and the activity of the WER-EGL3-TTG1 complex.

BR signaling promotes the N cell fate of root epidermis

Our data also support the suggestion that the N cell is a default cell type in root epidermis, and that H cell fate is produced due to inhibition of N cell fate by internal or external cues. First, we observed that WER, a positive regulator for GL2 expression, is expressed in both N and H cells in the early root meristem (Figure 8—figure supplement 1), which is also supported by a previous report that WER exhibits uniform promoter activity in both N and H cells proximal to the initial cells (Savage et al., 2008). Second, in Arabidopsis, both EGL3 and GL3 are expressed in H cells, but their proteins move to adjacent cells to promote N cell fate (Bernhardt et al., 2005). If they stay in H cells with the ability to interact with WER and TTG1, the H cells may develop into N cells as shown in the EGL3T209A/T213A-GFP transgenic plants. Moreover, over-expression of GL3 and EGL3 promoted non-hair cell fate (Bernhardt et al., 2003). Third, TTG1 is localized in both N and H cells, and TTG1 and EGL3 may synergistically promote WER-EGL3 transcriptional activity and enhance N cell fate. Apparently, BR signaling can promote N cell fate in several ways. Besides the inhibition of BR signaling on EGL3 cell–cell movement and the promotion of TTG1 activity, BR signaling also promotes WER expression as WER up-regulation in bin2-3 bil1 bil2 (Figure 8—figure supplement 1), which is consistent with the positive role of BRs in WER expression (Kuppusamy et al., 2009).

Thus, we proposed a model to illustrate how BR signaling regulates WER-EGL3-TTG1 complex formation and activity to control root epidermal cell fate. As shown in Figure 8, without BRs, WER-GL3/EGl3-TTG1 complex formation and activity is inhibited in both N and H cells, as WER expression is reduced in both H and N cells, and the activated GSK3-like kinases phosphorylate EGL3 in H cells to promote its cytoplasmic localization in both H cells and N cells, both of which lead to less WER-GL3/EGL3-TTG1 complex formation in nuclei and suppression of GL2 expression. The activity of some formed WER-bHLH-TTG1 complexes may be further inhibited by GSK3-like kinases phosphorylating TTG1. In contrast, enhanced BR early signaling inhibits GSK3-like kinases, leading to nuclear accumulation of the unphosphorylated EGL3 in H cells and normal function of unphosphorylated TTG1 in both cell types. Although CPC can move into H cells, due to enhanced WER expression and more efficient interaction of EGL3 with WER than with CPC (Song et al., 2011), more WER-EGL3-TTG1 complex is formed in H cells to promote GL2 expression and determine N cell fate. In N cells, the nucleus-localized GL3 can interact with WER and TTG1 to promote GL2 expression and maintain N cell fate. However, it remains to be investigated how TTG1 and WER expression is regulated by BR signaling. It is also not clear how BR signaling coordinates with positional signals and other phytohormones to regulate root hair patterning.

Figure 8 with 1 supplement see all
Download asset Open asset
A proposed model to illustrate how BR signaling regulates root epidermal cell fate.

Without BR early signaling, WER expression is reduced, and the activated GSK3-like kinases phosphorylate EGL3 and TTG1 in both H and N cells, leading to reduced formation and/or activity of the WER-EGL3/GL3-TTG1 complex, which inhibits GL2 expression in some N cells. With enhanced BR early signaling, WER expression is enhanced in both H and N cells, and the GSK3-like kinases activity is inhibited, leading to reduced phosphorylation of EGL3 and TTG1 in both cell types. Thus, WER-EGL3-TTG1 and WER-GL3-TTG1 complexes with transcriptional activity are formed in H and N cells, respectively, to promote GL2 expression and non-root hair cell fate. BR: brassinosteroid.

https://doi.org/10.7554/eLife.02525.018

Materials and methods

Plant materials and growth conditions

Request a detailed protocol

The seeds of the wer-1 and PGL2::GUS lines were obtained from Dr John Schiefelbein (University of Michigan), the bin2-3 bil1 bil2 seeds were obtained from Jianming Li (University of Michigan), and the cpc-1 seeds (CS6399) were obtained from the Arabidopsis Biological Resource Center (Ohio State University). Combinations of the BR-related mutants with the root hair mutants or the PGL2::GUS line were generated by crossing and selected by GUS staining based on the mutant phenotype, or antibiotic selection marker analysis. For root hair observation, seeds were grown on 1/2 MS medium (pH 5.8) with 1% sucrose, chilled for 3 d at 4°C, and grown for 5 d at 23°C under long-day conditions (16 hr light/8 hr dark). Nicotiana benthamiana plants were grown at 28°C under long-day conditions (16 hr light/8 hr dark).

Construction of double or multiple mutants

Request a detailed protocol

The double mutants or multiple mutants were derived from genetic crosses of the parental mutants (or transgenic lines). For generation of the BRI1-OX wer-1 and bil1 bil2 bil3 wer-1 double/multiple mutants, the wer-1 was genotyped with its point mutation-derived cleaved amplified polymorphic sequence (CAPS) marker (Lee and Schiefelbein, 1999) (Supplementary file 1), the bin2-3 bil1 bil2 was genotyped as described (Yan et al., 2009), and the BRI1-OX was selected by the antibiotic selection markers. For generation of bri1-116 cpc-1 and cpd cpc-1 double mutants, the cpc-1 was identified by PCR and phenotypic analysis, and the bri1-116 and the cpd were isolated by phenotype.

Plasmid construction and recombined protein purification

Request a detailed protocol

For GST pull-down assays, BIN2 was cloned into the PET28a vector, and EGL3 was cloned into the pGEX4T-1 vector. For in vitro kinase assays, WER, TTG1, CPC, and EGL3 were cloned into the pMAL-C2X vector. His-fused BIN2 (BIN2-His), GST-fused EGL3 (EGL3-GST), and MBP-fused WER (WER-MBP), TTG1 (TTG1-MBP), CPC (CPC-MBP), and EGL3 (EGL3-MBP) were expressed in BL21 (DE3) pLySs strain and purified with either Ni-NTA agarose (Clontech, Mountain View, CA), glutathione resin (Genescript, Piscataway, NJ), or amylase resin (NEB, Ipswich, MA), respectively.

Plant transformation and selection of transgenic plants

Request a detailed protocol

To generate plants expressing GFP-tagged EGL3 or mutated EGL3T399A and EGL3T209A/T213A, the various EGL3 cDNAs were cloned in-frame with GFP into the pCAMBIA2302 vector and driven by the EGL3 promoter (2 kb upstream of the start codon), and the plants expressing GFP-tagged TTG1 were generated by cloning the TTG1 cDNA in-frame with GFP into the pCAMBIA2302 vector and driven by the TTG1 promoter (2 kb upstream of the start codon). The constructs were transformed into Agrobacterium tumefaciens GV3101 strains. All transgenic plants were generated by floral dip transformation. T0 seeds were harvested and screened by germinating on MS solid medium with antibiotic selection. For each transformation, at least five individual T1 transgenic lines were selected. Transgenic lines of PEGL3::EGL3-GFP, PEGL3::EGL3T399A-GFP, PEGL3::EGL3T209A/T213A-GFP, and PTTG1::TTG1-GFP with T2 or higher generations were used for further analysis.

Microscopy and histochemical analysis

Request a detailed protocol

The root hair pattern of the 5-day-old seedlings was observed, and images at ×100 magnification were taken with a Leica MZ FLIII stereomicroscope (Leica Microsystems). The root hair density was counted as described (Galway et al., 1994; Jones et al., 2002) with some modifications. Any visible protrusion from the epidermal cell was regarded as a root hair, regardless of length. The number of root hairs was counted from one side of a 1 mm segment from the imitated differentiation region of the 5-day-old roots, and at least eight roots were measured for each stain. The hair cell length was measured along the longitudinal plane at ×100 magnification using the software Scion Image, and at least 10 root hair cells were measured for each root. The relative root number was calculated as root hair density × root hair cell length for each root as described (Wada et al., 1997).

The histochemical staining of 5-day-old roots harboring the GUS reporter was performed as described (Masucci et al., 1996). Transverse sections of root meristem were prepared as described (Ye et al., 2010) with modifications. The proportion of cells expressing or not expressing PGL2::GUS reporter in H cells or N cells was measured by examining sections at least from eight seedlings in each strain. For protein localization of EGL3-GFP and its mutated forms, the 5-day-old transgenic plants were examined by confocal microscope (Zeiss) after staining with 5 μg/ml propidium iodide (PI) (Sigma, St. Louis, MO) for 10 s at room temperature, and images were captured at 489 nm and 538 nm laser excitation and at 509 nm and 617 nm emission for GFP and PI staining. The pattern of epidermal cell types was determined as described (Lee and Schiefelbein, 2002).

Yeast two-hybrid assay

Request a detailed protocol

For yeast two-hybrid assays, the full length cDNA of BIN2 was cloned into vector pEXP-AD502 (BIN2-AD) and used as a prey, and the full length cDNAs of EGL3, WER, TTG1, and CPC were cloned into the pDBLeu vector (EGL3-DB, WER-DB, TTG1-DB, CPC-DB), respectively, and used as a bait. The prey and bait plasmids were transformed into the yeast strains AH109 and Y187, respectively. After yeast mating, the protein–protein interactions were tested on SD medium minus Leu, Trp, and His, and containing 2 mM 3-amino-1, 2, 4-triazole (3AT) (Sigma, St. Louis, MO).

Transient expression assays in Nicotiana benthamiana leaves

Request a detailed protocol

To generate the vector system for BiFC analysis, the full length cDNAs of EGL3, WER, TTG1, and CPC were cloned into the pXY104 vector (cYFP), respectively, to generate EGL3-cYFP, WER-cYFP, TTG1-cYFP, and CPC-cYFP constructs, and BIN2 cDNA was cloned into the pXY106 (nYFP) vector to generate BIN2-nYFP construct. For transient expression, Agrobacterium strains (GV3101) carrying the constructs for testing the specific interaction were transformed into 4–5-week-old Nicotiana benthamiana leaves as described previously (Walter et al., 2004). After infiltration for 4 d, the lower leaf epidermis cells were used for analyzing the fluorescence signal by confocal microscopy (Zeiss).

For dual-luciferase assays, cDNAs of the effectors WER, EGL3, and TTG1, and the regulator bin2-1 were cloned with Flag tag into pCAMBIA2302 driven by a 35S promoter. GL2 promoter (2 kb upstream of the start codon) was cloned into the pGreenII 0800-LUC vector to be used as the reporter. The method of transient expression used was as previously described (Hellens et al., 2005).

GST pull-down assays

Request a detailed protocol

The purified proteins, EGL3-GST, WER-GST, TTG1-GST, CPC-GST, and GST, were bound with 25 μl GST resin in binding buffer (10 mM phosphate buffer, pH 7.4, 140 mM NaCl, 3 mM KCl, 0.1% Triton X-100) for 2 hr at 4°C. After washing three times with the binding buffer, an equal amount of BIN2-His was added and rebound for 2 hr at 4°C. After boiling in SDS loading buffer for 5 min, the pull-down proteins were separated on 10% SDS–PAGE gels and detected by immunoblotting with anti-His antibody (Abmart, Shanghai, China).

In vitro kinase assays and phosphorylation site identification

Request a detailed protocol

In vitro kinase assays were performed in 24 μl reaction buffer (20 mM Tris, pH 7.5, 10 mM MgCl2, 5 mM DTT) containing 20 μM ATP and 1 μl of 10 μCi [32P] γATP (PerkinElmer, Waltham, Massachusetts) and purified proteins. The reaction was carried out at 30°C for 1 hr and terminated by adding 6 μl of 5 × SDS loading buffer. After boiling for 3 min, proteins were separated on 10% SDS–PAGE. Gels were stained with Coomassie brilliant blue, and then dried and autoradiographed. For phosphorylation site identification, in vitro kinase assays were performed, and protein bands were excised to be used for mass spectrometry analysis.

Site-directed mutagenesis of EGL3

Request a detailed protocol

The mutated forms of EGL3 were generated by a PCR-based site-directed mutagenesis (Supplementary file 1).

References

    1. Cohen P
    2. Frame S
    (2001) The renaissance of GSK3
    Nature Reviews Molecular Cell Biology 2:769–776.
    https://doi.org/10.1038/35096075
    1. Masucci JD
    2. Rerie WG
    3. Foreman DR
    4. Zhang M
    5. Galway ME
    6. Marks MD
    7. Schiefelbein JW
    (1996)
    The homeobox gene GLABRA2 is required for position-dependent cell differentiation in the root epidermis of Arabidopsis thaliana
    Development (Cambridge, England) 122:1253–1260.

Article and author information

Author details

  1. Yinwei Cheng

    State Key Laboratory of Genetic Engineering, and Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai, China
    Contribution
    YC, Conception and design, Acquisition of data, Analysis and interpretation of data, Drafting or revising the article
    Competing interests
    The authors declare that no competing interests exist.

  2. Wenjiao Zhu

    State Key Laboratory of Genetic Engineering, and Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai, China
    Contribution
    WZ, Acquisition of data, Analysis and interpretation of data
    Competing interests
    The authors declare that no competing interests exist.

  3. Yuxiao Chen

    State Key Laboratory of Genetic Engineering, and Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai, China
    Contribution
    YC, Acquisition of data, Analysis and interpretation of data
    Competing interests
    The authors declare that no competing interests exist.

  4. Shinsaku Ito

    Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan
    Contribution
    SI, Contributed unpublished essential data or reagents
    Competing interests
    The authors declare that no competing interests exist.

  5. Tadao Asami

    Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan
    Contribution
    TA, Contributed unpublished essential data or reagents
    Competing interests
    The authors declare that no competing interests exist.

  6. Xuelu Wang

    1. State Key Laboratory of Genetic Engineering, and Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai, China
    2. College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China
    Contribution
    XW, Conception and design, Analysis and interpretation of data, Drafting or revising the article
    For correspondence
    xueluw@gmail.com
    Competing interests
    The authors declare that no competing interests exist.

Funding

National Basic Research Program of China (2012CB114300)

  • Xuelu Wang

National Natural Science Foundation of China (91317302)

  • Xuelu Wang

National Natural Science Foundation of China (30925020)

  • Xuelu Wang

Shanghai Science and Technology Committee (10JC1400800)

  • Xuelu Wang

The funder had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Acknowledgements

We thank John Schiefelbein and Jianming Li (University of Michigan) for providing seeds, Ramin Yadegari (University of Arizona) and Hongquan Yang (Shanghai Jiao Tong University) for reviewing drafts of our manuscript, Hexige Saiyin and Donghong Chen (Fudan University) for providing advice on paraffin sections, Youheng Wei (Fudan University) for technical advice on confocal images, and Jianjun Jiang (Fudan University) for helpful discussions on the function of nuclear localization signals.

Copyright

© 2014, Cheng et al.

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

Metrics

  • 4,031
    views
  • 759
    downloads
  • 118
    citations

Views, downloads and citations are aggregated across all versions of this paper published by eLife.

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Open citations (links to open the citations from this article in various online reference manager services)

Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)

  1. Yinwei Cheng
  2. Wenjiao Zhu
  3. Yuxiao Chen
  4. Shinsaku Ito
  5. Tadao Asami
  6. Xuelu Wang
(2014)
Brassinosteroids control root epidermal cell fate via direct regulation of a MYB-bHLH-WD40 complex by GSK3-like kinases
eLife 3:e02525.
https://doi.org/10.7554/eLife.02525

Share this article

https://doi.org/10.7554/eLife.02525

Categories and tags

Research organism

Further reading

    1. Microbiology and Infectious Disease
    2. Plant Biology

    Root cap cell corpse clearance limits microbial colonization in Arabidopsis thaliana

    Nyasha Charura, Ernesto Llamas ... Alga Zuccaro
    Research Article

    Programmed cell death occurring during plant development (dPCD) is a fundamental process integral for plant growth and reproduction. Here, we investigate the connection between developmentally controlled PCD and fungal accommodation in Arabidopsis thaliana roots, focusing on the root cap-specific transcription factor ANAC033/SOMBRERO (SMB) and the senescence-associated nuclease BFN1. Mutations of both dPCD regulators increase colonization by the beneficial fungus Serendipita indica, primarily in the differentiation zone. smb-3 mutants additionally exhibit hypercolonization around the meristematic zone and a delay of S. indica-induced root-growth promotion. This demonstrates that root cap dPCD and rapid post-mortem clearance of cellular corpses represent a physical defense mechanism restricting microbial invasion of the root. Additionally, reporter lines and transcriptional analysis revealed that BFN1 expression is downregulated during S. indica colonization in mature root epidermal cells, suggesting a transcriptional control mechanism that facilitates the accommodation of beneficial microbes in the roots.

    1. Cell Biology
    2. Plant Biology

    Autophagosome development and chloroplast segmentation occur synchronously for piecemeal degradation of chloroplasts

    Masanori Izumi, Sakuya Nakamura ... Shinya Hagihara
    Research Article

    Plants distribute many nutrients to chloroplasts during leaf development and maturation. When leaves senesce or experience sugar starvation, the autophagy machinery degrades chloroplast proteins to facilitate efficient nutrient reuse. Here, we report on the intracellular dynamics of an autophagy pathway responsible for piecemeal degradation of chloroplast components. Through live-cell monitoring of chloroplast morphology, we observed the formation of chloroplast budding structures in sugar-starved leaves. These buds were then released and incorporated into the vacuolar lumen as an autophagic cargo termed a Rubisco-containing body. The budding structures did not accumulate in mutants of core autophagy machinery, suggesting that autophagosome creation is required for forming chloroplast buds. Simultaneous tracking of chloroplast morphology and autophagosome development revealed that the isolation membranes of autophagosomes interact closely with part of the chloroplast surface before forming chloroplast buds. Chloroplasts then protrude at the site associated with the isolation membranes, which divide synchronously with autophagosome maturation. This autophagy-related division does not require DYNAMIN-RELATED PROTEIN 5B, which constitutes the division ring for chloroplast proliferation in growing leaves. An unidentified division machinery may thus fragment chloroplasts for degradation in coordination with the development of the chloroplast-associated isolation membrane.

    1. Plant Biology

    Structural basis for molecular assembly of fucoxanthin chlorophyll a/c-binding proteins in a diatom photosystem I supercomplex

    Koji Kato, Yoshiki Nakajima ... Ryo Nagao
    Research Article

    Photosynthetic organisms exhibit remarkable diversity in their light-harvesting complexes (LHCs). LHCs are associated with photosystem I (PSI), forming a PSI-LHCI supercomplex. The number of LHCI subunits, along with their protein sequences and pigment compositions, has been found to differ greatly among the PSI-LHCI structures. However, the mechanisms by which LHCIs recognize their specific binding sites within the PSI core remain unclear. In this study, we determined the cryo-electron microscopy structure of a PSI supercomplex incorporating fucoxanthin chlorophyll a/c-binding proteins (FCPs), designated as PSI-FCPI, isolated from the diatom Thalassiosira pseudonana CCMP1335. Structural analysis of PSI-FCPI revealed five FCPI subunits associated with a PSI monomer; these subunits were identified as RedCAP, Lhcr3, Lhcq10, Lhcf10, and Lhcq8. Through structural and sequence analyses, we identified specific protein–protein interactions at the interfaces between FCPI and PSI subunits, as well as among FCPI subunits themselves. Comparative structural analyses of PSI-FCPI supercomplexes, combined with phylogenetic analysis of FCPs from T. pseudonana and the diatom Chaetoceros gracilis, underscore the evolutionary conservation of protein motifs crucial for the selective binding of individual FCPI subunits. These findings provide significant insights into the molecular mechanisms underlying the assembly and selective binding of FCPIs in diatoms.