1. Cell Biology
  2. Plant Biology
Download icon

Biosensors: Plant biologists FRET over stress

  1. Won-Gyu Choi
  2. Simon Gilroy  Is a corresponding author
  1. University of Wisconsin, United States
Cite this article as: eLife 2014;3:e02763 doi: 10.7554/eLife.02763
1 figure


Protein bioprobes allow the imaging of changing abscisic acid (ABA) levels in living plants.

The biosensors consist of a single protein containing cyan fluorescent protein, an ABA receptor region, a protein region that naturally binds to the ABA-activated receptor, and yellow fluorescent protein. Cyan fluorescent protein is excited by blue-violet light. For the ABACUS bioprobes, binding to ABA causes the protein to fold up and brings the cyan and yellow fluorescent proteins into close proximity. This increases the efficiency of FRET (cyan arrow), and shifts the colour of fluorescence from more cyan to more yellow (Jones et al., 2014). The ABAleon bioprobes are designed the same way, but work differently in that binding to ABA actually shifts the fluorescence the other way: from more yellow to more cyan (Waadt et al., 2014).

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Download citations (links to download the citations from this article in formats compatible with various reference manager tools)

Open citations (links to open the citations from this article in various online reference manager services)