(A) Historical crossover frequency (red, cM/Mb) and sequence diversity (π, blue) along the physical length of the Arabidopsis thaliana chromosomes (Mb) (Cao et al., 2011; Choi et al., 2013). Mean …
(A) Combined red and green, red alone and green alone fluorescent micrographs of seed from a self-fertilized 420/++ plant. (B) CellProfiler output showing identification of seed objects by green …
420 crossover frequency measured via manual or automated scoring of seed fluorescence.
(A) Genetic diagram illustrating generation of F2 plants heterozygous for the 420 fluorescent transgenes, annotated as in Figure 1C. F2 plants heterozygous for the fluorescent transgenes can occur …
(A–E) Genetic distance (cM) measurements for fluorescent crossover intervals I1b, I1fg, I2f, 420 and CEN3 with individual replicates (black dots) and mean values (red dots) for the crosses labelled …
I1b F1 flow cytometry count data.
I1b F1 flow cytometry count data.
I1b F1 flow cytometry count data.
I1b F1 flow cytometry count data.
CEN3 F1 flow cytometry count data.
(A) Diagram illustrating chromosome 3 genotypes (black = Col, red = Ct) in RG/++ F1 individuals and their F2 progeny. A single chromosome is shown for simplicity. Gametes or progeny are analysed for …
420 Col/Ct F2 fluorescent seed count data.
I2f Col/Ct F2 fluorescent seed count data.
CEN3 Col/Ct F2 flow cytometry count data.
(A) CEN3 cM from Col/Ct CEN3/++ F2 (black), Col/Col homozygotes (red) and Col/Ct F1 (blue) individuals. Horizontal dotted lines indicate mean value. See Figure 4—source data 3. (B) Chromosome 3 …
(A) Schematic diagram illustrating the physical location of 420 and I3bc transgenes expressing fluorescent proteins in seed and pollen. Beneath are diagrams illustrating the locations of Col/Col …
Three colour I3bc FTL flow cytometry count data.
Three colour I3bc FTL flow cytometry count data–measurement of crossover interference.
Flow cytometry plots are shown measuring pollen for the indicated colour of fluorescent protein. In the upper plot total hydrated pollen is divided into blue and non-blue populations using polygonal …
(A–D) Replicate measurements of 420 (red) and CEN3 (blue) genetic distances (cM) are plotted in wild type, fancm and fancm zip4. See Figure 6—source data 1, 2. Chromosome 3 genotypes of the plants …
420 fluorescent seed count data from wild type, fancm and fancm zip4 individuals with varying heterozygosity.
CEN3 flow cytometry count data from wild type, fancm and fancm zip4 individuals with varying heterozygosity.
Diagram showing the crossing scheme used to generate plants to test the requirement of recombination pathways in crossover remodelling. At relevant points the genotype of chromosome 3 is illustrated …
(A–E) Metaphase-I chromosome spreads from anthers from (A) Col/Col 420, (B) Ct/Ct, (C) Col × Ct F1, (D) a Col × Ct 420 (HOM-HET) cold recombinant line and (E) a Col × Ct 420 hot (HET-HOM) …
Chiasmata count data.
(A–D) Replicate measurements of I3b and I3c genetic distances (cM), and I3bc crossover interference are plotted in wild type, fancm, fancm zip4 and zip4. Black dots represent replicate measurements …
I3bc fluorescent seed count data from wild type, fancm and fancm zip4 individuals with varying heterozygosity.
Calculation of I3bc interference from wild type, fancm and fancm zip4 individuals with varying heterozygosity.
I3bc fluorescent seed count data from wild type and zip4 individuals with varying heterozygosity.
Calculation of I3bc interference in wild type and zip4.
Diagram showing the crossing scheme used to generate plants to investigate the impact of the Col/Ct heterozygosity on crossover interference. Genotypes differing in polymorphism pattern for crosses …
Correlations between historical recombination and sequence diversity at varying physical scales
Scale (π) | Chr1 | Chr2 | Chr3 | Chr4 | Chr5 |
---|---|---|---|---|---|
5 kb | 0.521 | 0.301 | 0.545 | 0.575 | 0.541 |
10 kb | 0.556 | 0.305 | 0.565 | 0.602 | 0.562 |
50 kb | 0.657 | 0.381 | 0.579 | 0.692 | 0.619 |
100 kb | 0.699 | 0.563 | 0.601 | 0.744 | 0.646 |
500 kb | 0.741 | 0.528 | 0.615 | 0.841 | 0.653 |
1 Mb | 0.639 | 0.504 | 0.683 | 0.846 | 0.624 |
Scale (θ) | Chr1 | Chr2 | Chr3 | Chr4 | Chr5 |
---|---|---|---|---|---|
5 kb | 0.537 | 0.298 | 0.557 | 0.585 | 0.553 |
10 kb | 0.569 | 0.303 | 0.576 | 0.610 | 0.572 |
50 kb | 0.662 | 0.382 | 0.592 | 0.699 | 0.623 |
100 kb | 0.710 | 0.573 | 0.617 | 0.752 | 0.650 |
500 kb | 0.754 | 0.534 | 0.635 | 0.844 | 0.655 |
1 Mb | 0.647 | 0.504 | 0.697 | 0.849 | 0.635 |
Spearman's rank correlation between historical crossover frequency estimates from LDhat and sequence diversity (θ and π) at varying physical scales (Cao et al., 2011; Choi et al., 2013). Adjacent windows of the indicated physical size were used for correlations.
Fluorescent crossover reporter intervals
Interval | Chr | Method | T-DNA 1 | T-DNA 2 | Mb | Location | cM/Mb (Col-0) | cM/Mb (F1) | Heterozygosity |
---|---|---|---|---|---|---|---|---|---|
I1b | 1 | Pollen | 3,905,441-YFP | 5,755,618-dsRed2 | 1.85 | Interstitial | 4.25 | 4.05 | 1.93 (3.16) |
I1c | 1 | Pollen | 5,755,618-dsRed2 | 9,850,022-CFP | 4.09 | Interstitial | 4.55 | N/A | 2.80 (3.16) |
I1fg | 1 | Pollen | 24,645,163-YFP | 25,956,590-dsRed2 | 1.31 | Interstitial | 6.20 | 6.02 | 2.52 (3.16) |
I2a | 2 | Pollen | 12,640,092-CFP | 13,226,013-YFP | 0.59 | Interstitial | 5.19 | N/A | 2.33 (3.30) |
I2b | 2 | Pollen | 13,226,013-YFP | 14,675,407-dsRed2 | 1.45 | Interstitial | 3.09 | N/A | 1.53 (3.30) |
I2f | 2 | Pollen | 18,286,716-dsRed2 | 18,957,093-YFP | 0.67 | Sub-telomeric | 13.02 | 17.41 | 1.43 (3.30) |
420 | 3 | Seed | 256,516-GFP | 5,361,637-dsRed2 | 5.11 | Sub-telomeric | 3.70 | 2.93 | 1.19 (3.37) |
CEN3 | 3 | Pollen | 11,115,724-YFP | 16,520,560-dsRed2 | 5.40 | Centromeric | 2.11 | 2.38 | 6.69 (3.37) |
I3b | 3 | Pollen | 498,916-CFP | 3,126,994-YFP | 2.63 | Sub-telomeric | 5.99 | N/A | 1.11 (3.37) |
I3c | 3 | Pollen | 3,126,994-YFP | 4,319,513-dsRed2 | 1.19 | Sub-telomeric | 4.01 | N/A | 1.64 (3.37) |
I5c | 5 | Pollen | 2,372,623-CFP | 3,760,756-YFP | 1.39 | Interstitial | 4.01 | N/A | 1.01 (3.27) |
I5d | 5 | Pollen | 3,760,756-YFP | 5,497,513-dsRed2 | 1.74 | Interstitial | 3.20 | N/A | 1.56 (3.27) |
The interval name is listed together with chromosome, method of scoring and location of the flanking T-DNAs together with the fluorescent proteins they encode. Interval cM/Mb values from Col-0 homozygous are listed (Col-0), in addition to the mean cM/Mb observed across all F1 crosses (F1). Heterozygosity values were calculated using pairwise comparison of polymorphism data from the 19 genomes project to the Col reference (Gan et al., 2011), and the mean value for the interval shown, in addition to the mean chromosome value in parentheses.
Genetic distance in F1 heterozygotes
Accession | Location | I1b | I1fg | I2f | 420 | CEN3 | Total | P |
---|---|---|---|---|---|---|---|---|
Tsu-0 | Tsushima, Japan | 6.6 | 6.3 | 6.9 | 14.5 | 9.4 | 43.7 | <2.00 × 10−16 |
Hi-0 | Hilversum, Netherlands | 6.8 | 6.9 | 6.9 | 13.6 | 9.6 | 43.8 | <2.00 × 10−16 |
Wil-2 | Vilnius, Lithuania | 6.1 | 6.9 | 6.1 | 15.9 | 10.1 | 45.0 | <2.00 × 10−16 |
Kn-0 | Kaunas, Lithuania | 7.4 | 6.6 | 8.0 | 15.5 | 8.7 | 46.2 | <2.00 × 10−16 |
Ler-0 | Gorzow, Poland | 6.6 | 8.2 | 7.6 | 12.3 | 11.9 | 46.6 | <2.00 × 10−16 |
Ws-0 | Vassilyevichy, Belarus | 6.7 | 7.7 | 10.2 | 13.0 | 9.0 | 46.6 | <2.00 × 10−16 |
No-0 | Nossen, Germany | 7.4 | 7.9 | 6.7 | 14.1 | 11.4 | 47.4 | <2.00 × 10−16 |
Wu-0 | Wurzburg, Germany | 7.6 | 6.3 | 9.5 | 14.0 | 11.4 | 48.8 | <2.00 × 10−16 |
Zu-0 | Zurich, Switzerland | 7.5 | 7.1 | 13.4 | 12.2 | 9.9 | 50.1 | 0.0438 |
Po-0 | Poppelsdorf, Germany | 7.2 | 7.9 | 9.1 | 15.8 | 10.9 | 51.0 | 0.000484 |
Ct-1 | Catania, Italy | 7.8 | 8.7 | 7.2 | 15.9 | 12.1 | 51.7 | 9.27 × 10−08 |
Oy-0 | Oystese, Norway | 7.7 | 8.4 | 8.5 | 15.7 | 12.5 | 52.8 | 0.969 |
Rsch-4 | Rschew, Russia | 7.9 | 6.8 | 10.7 | 15.2 | 12.4 | 53.1 | 0.505 |
Col-0 | Columbia, USA | 8.0 | 8.2 | 8.8 | 18.0 | 11.5 | 54.5 | – |
Sf-2 | San Feliu, Spain | 8.2 | 8.8 | 7.4 | 18.6 | 12.3 | 55.3 | 0.724 |
Kas | Kashmir, India | 6.9 | 8.6 | 13.2 | 13.8 | 13.3 | 55.8 | <2.00 × 10−16 |
Kond | Pugus, Tajikistan | 7.1 | 8.1 | 15.8 | 13.7 | 11.4 | 56.2 | <2.00 × 10−16 |
Edi-0 | Edinburgh, Scotland | 8.0 | 8.0 | 13.4 | 13.3 | 13.6 | 56.3 | <2.00 × 10−16 |
Bay-0 | Bayreuth, Germany | 8.6 | 8.3 | 11.3 | 18.6 | 11.5 | 58.3 | <2.00 × 10−16 |
Mt-0 | Martuba, Libya | 9.6 | 7.8 | 13.2 | 20.6 | 9.6 | 60.8 | <2.00 × 10−16 |
Sha | Pamiro-Alaya, Tajikistan | 7.8 | 7.5 | 20.0 | 7.0 | 18.6 | 60.9 | 0.0012 |
C24 | Columbia, USA | 8.8 | 8.5 | 18.5 | 12.1 | 14.1 | 61.9 | <2.00 × 10−16 |
Bur-0 | Burren, Ireland | 6.7 | 9.1 | 21.9 | 14.7 | 17.8 | 70.2 | <2.00 × 10−16 |
Cvi-0 | Cape Verde Islands | 9.1 | 10.0 | 11.3 | 12.6 | 27.6 | 70.7 | <2.00 × 10−16 |
Can-0 | Las Palmas, Canary Isles | 7.8 | 8.5 | 22.1 | 12.4 | 31.4 | 82.2 | <2.00 × 10−16 |
Co | Coimbra, Portugal | – | – | – | 11.1 | 13.8 | – | – |
Nw-0 | Neuweilnau, Germany | – | – | – | 14.7 | 14.4 | – | – |
Mh-0 | Szczecin, Poland | – | – | – | 14.9 | 10.1 | – | – |
Wl-0 | Wildbad, Germany | – | – | – | 17.0 | 9.5 | – | – |
Bu-0 | Burghaun, Germany | – | – | – | 28.9 | 8.8 | – | – |
CIBC5 | Ascot, United Kingdom | – | – | – | 13.2 | 11.3 | – | – |
RRS7 | North Liberty, USA | – | – | – | 17.2 | 11.7 | – | – |
F1 cM mean | 7.6 | 7.9 | 11.5 | 15.0 | 12.9 | 54.8 | ||
cM StDev | 0.8 | 0.9 | 4.8 | 3.6 | 4.9 | 9.3 |
The accessions crossed to are listed with their geographic location. Genetic distance (cM) data is shown for the five fluorescent intervals, in addition to a summed total. Also shown are the mean and standard deviation for all F1s. A generalized linear model (GLM) was used to test for significant differences between total recombinant vs non-recombinant counts between replicate groups of Col-0 homozygotes and F1 heterozygotes. Tests were performed for genotypes where data from all five tested intervals had been collected.
F1 heterozygosity levels relative to Col-0
Accession | Chr 1 | I1b | I1fg | Chr 2 | I2f | Chr 3 | 420 | CEN3 | Chr 4 | Chr 5 |
---|---|---|---|---|---|---|---|---|---|---|
Bur-0 | 3.35 | 1.86 | 3.62 | 3.60 | 1.51 | 3.58 | 1.57 | 6.20 | 3.89 | 3.16 |
Can-0 | 3.75 | 2.99 | 3.51 | 3.92 | 0.92 | 3.98 | 1.02 | 8.27 | 5.34 | 4.24 |
Ct-1 | 2.62 | 1.67 | 2.29 | 2.61 | 1.85 | 3.35 | 0.96 | 6.91 | 3.23 | 3.36 |
Edi-0 | 3.30 | 1.91 | 3.64 | 3.26 | 0.91 | 3.05 | 1.34 | 5.48 | 3.42 | 3.81 |
Hi-0 | 2.43 | 1.59 | 1.87 | 1.80 | 1.50 | 2.58 | 1.07 | 4.62 | 2.69 | 2.46 |
Kn-0 | 3.15 | 1.78 | 2.85 | 3.35 | 2.18 | 3.58 | 1.49 | 6.69 | 3.76 | 3.40 |
Ler-0 | 3.10 | 1.61 | 2.66 | 3.62 | 2.24 | 3.43 | 1.13 | 7.39 | 3.87 | 3.53 |
Mt-0 | 3.02 | 1.77 | 1.16 | 3.49 | 1.57 | 3.17 | 1.07 | 5.70 | 3.95 | 2.71 |
No-0 | 3.25 | 2.28 | 2.71 | 3.36 | 1.27 | 3.52 | 1.21 | 7.14 | 3.51 | 3.56 |
Oy-0 | 3.48 | 1.68 | 2.10 | 3.05 | 0.58 | 2.94 | 1.23 | 6.16 | 2.95 | 2.72 |
Po-0 | 2.45 | 1.78 | 1.19 | 2.36 | 0.67 | 2.87 | 0.79 | 5.99 | 2.53 | 2.59 |
Rsch-4 | 2.94 | 1.84 | 1.17 | 3.36 | 1.22 | 3.09 | 1.05 | 5.37 | 3.89 | 3.22 |
Sf-2 | 3.61 | 1.94 | 4.24 | 3.54 | 2.06 | 3.74 | 1.30 | 8.24 | 3.81 | 3.58 |
Tsu-0 | 3.37 | 1.68 | 2.36 | 3.69 | 1.39 | 3.98 | 1.14 | 8.78 | 3.69 | 3.05 |
Wil-2 | 3.56 | 1.99 | 2.45 | 3.77 | 2.11 | 3.81 | 1.56 | 7.55 | 4.44 | 3.34 |
Ws-0 | 3.25 | 1.93 | 3.54 | 3.68 | 1.58 | 3.30 | 1.30 | 6.65 | 3.70 | 3.41 |
Wu-0 | 3.13 | 2.53 | 1.95 | 3.14 | 0.67 | 3.50 | 1.22 | 7.41 | 3.36 | 3.15 |
Zu-0 | 3.10 | 1.85 | 2.02 | 3.83 | 1.43 | 3.19 | 0.96 | 5.84 | 3.38 | 3.64 |
Mean | 3.16 | 1.93 | 2.52 | 3.30 | 1.43 | 3.37 | 1.19 | 6.69 | 3.63 | 3.27 |
Correlation (cM) | – | 0.13 (p = 0.61) | 0.47 (p = 0.05) | – | −0.29 (p = 0.23) | – | 0.06 (p = 0.81) | 0.28 (p = 0.26) | – | – |
Accessions sequenced as part of the 19 genomes project were analysed (Gan et al., 2011) and heterozygosity calculated as the sum of SNPs and indel lengths divided by the length of region (kb). Correlations were between heterozygosity within the interval measured and F1 cM measurements.
Chromosome 3 genotype counts from hot and cold quartile 420/++ Col/Ct F2 individuals
Marker coordinates (bp) | Hot quartile HET | Hot quartile HOM | Cold quartile HET | Cold quartile HOM | FDR p value |
---|---|---|---|---|---|
259000 | 34 | 0 | 34 | 0 | 1 |
2718000 | 34 | 0 | 34 | 0 | 1 |
5352000 | 34 | 0 | 34 | 0 | 1 |
6375000 | 21 | 13 | 34 | 0 | 4.36 × 10−04 |
6948000 | 17 | 17 | 33 | 1 | 1.05 × 10−04 |
7674000 | 15 | 19 | 33 | 1 | 2.12 × 10−05 |
8495000 | 12 | 22 | 34 | 0 | 3.65 × 10−07 |
9404000 | 8 | 26 | 33 | 1 | 3.79 × 10−08 |
10695000 | 8 | 26 | 30 | 4 | 1.36 × 10−06 |
11649000 | 11 | 23 | 27 | 7 | 4.36 × 10−04 |
12356000 | 11 | 23 | 27 | 7 | 4.36 × 10−04 |
15949000 | 13 | 21 | 23 | 11 | 4.48 × 10−02 |
19165000 | 17 | 17 | 21 | 13 | 0.591 |
23040000 | 13 | 21 | 17 | 17 | 0.591 |
The number of 420/++ Col/Ct F2 individuals showing Col homozygosity (HOM) or Col/Ct heterozygosity (HET) for the indicated marker positions, in either the hottest or coldest F2 quartile. The p value was obtained by performing a chi square test between homozygous and heterozygous marker genotype counts in the hottest and coldest quartiles (2x2 contingency table), followed by FDR correction for multiple testing.
Chromosome 2 genotype counts from hot and cold quartile I2f/++ Col/Ct F2 individuals
Marker coordinates (bp) | Hot quartile HET | Hot quartile HOM | Cold quartile HET | Cold quartile HOM | FDR p value |
---|---|---|---|---|---|
132,000 | 9 | 11 | 8 | 12 | 1 |
2,346,000 | 7 | 13 | 8 | 12 | 1 |
4,748,000 | 8 | 12 | 9 | 11 | 1 |
6,789,000 | 7 | 13 | 11 | 9 | 0.63 |
11,443,000 | 5 | 15 | 20 | 0 | 6.26 × 10−05 |
13,036,000 | 7 | 13 | 20 | 0 | 3.32 × 10−04 |
14,117,000 | 9 | 11 | 20 | 0 | 1.30 × 10−03 |
15,240,000 | 9 | 11 | 20 | 0 | 1.30 × 10−03 |
16,909,000 | 13 | 7 | 20 | 0 | 0.0262 |
17,439,000 | 16 | 4 | 20 | 0 | 0.238 |
18,287,000 | 20 | 0 | 20 | 0 | 1 |
18,960,000 | 20 | 0 | 20 | 0 | 1 |
19,311,000 | 18 | 2 | 20 | 0 | 0.764 |
The number of I2f/++ Col/Ct F2 individuals showing Col homozygosity (HOM) or Col/Ct heterozygosity (HET) for the indicated markers, in either the hottest or coldest F2 quartile. The p value was obtained by performing a chi square test between homozygous and heterozygous marker genotype counts in the hottest and coldest quartiles (2 × 2 contingency table), followed by FDR correction for multiple testing.
Chromosome 3 genotype counts from hot and cold quartile CEN3/++ Col/Ct F2 individuals
Marker coordinates (bp) | Hot quartile HET | Hot quartile HOM | Cold quartile HET | Cold quartile HOM | FDR P |
---|---|---|---|---|---|
259000 | 16 | 14 | 17 | 13 | 1 |
2718000 | 16 | 14 | 18 | 12 | 1 |
5352000 | 19 | 11 | 17 | 13 | 1 |
7674000 | 20 | 10 | 12 | 18 | 0.129 |
8495000 | 23 | 7 | 13 | 17 | 0.0389 |
9404000 | 26 | 4 | 16 | 14 | 0.0308 |
11115724 | 30 | 0 | 30 | 0 | 1 |
16520560 | 30 | 0 | 30 | 0 | 1 |
21008000 | 27 | 3 | 14 | 16 | 0.00477 |
22076000 | 23 | 7 | 12 | 18 | 0.0308 |
23040000 | 24 | 6 | 10 | 20 | 0.00477 |
The number of CEN3/++ Col/Ct F2 individuals showing Col homozygosity (HOM) or Col/Ct heterozygosity (HET) for the indicated markers, in either the hottest or coldest quartile. The p value was obtained by performing a chi square test between homozygous and heterozygous marker genotype counts in the hottest and coldest quartiles (2 × 2 contingency table), followed by FDR correction for multiple testing.
Tetrad FTL cM data in Col/Col and Col/Ler backgrounds
Col/Col | Col/Ler | |||||||
---|---|---|---|---|---|---|---|---|
Interval | PD | NPD | T | cM* | PD | NPD | T | cM* |
1b | 3976 | 3 | 742 | 8.05 ± 0.29 | 4395 | 2 | 652 | 6.58 ± 0.25† |
1c | 3022 | 11 | 1695 | 18.62 ± 0.04 | 3156 | 18 | 1891 | 19.73 ± 0.04 |
2a | 6787 | 2 | 430 | 3.06 ± 0.15 | 5920 | 0 | 283 | 2.28 ± 0.13† |
2b | 6582 | 2 | 635 | 4.48 ± 0.18 | 5796 | 0 | 407 | 3.28 ± 0.16† |
3b | 4363 | 22 | 2557 | 19.37 ± 0.35 | 2758 | 2 | 1056 | 13.99 ± 0.38† |
3c | 6185 | 5 | 736 | 5.53 ± 0.21 | 3576 | 2 | 238 | 3.28 ± 0.22† |
5c | 5356 | 1 | 666 | 5.58 ± 0.21 | 5458 | 0 | 676 | 5.51 ± 0.20 |
5d | 5358 | 1 | 664 | 5.56 ± 0.21 | 5540 | 2 | 594 | 4.94 ± 0.20† |
Map distance in cM (±S.E.).
Significant difference in map distance in the heterozygous Col/Ler background compared to the same interval in the Col/Col homozygous background.
Tetrad FTL crossover interference data in Col/Col and Col/Ler backgrounds
Col/Col | Col/Ler | |||||
---|---|---|---|---|---|---|
Interval | W/o adj. CO* | w/ adj. CO* | R1† | W/o adj. CO* | w/ adj. CO* | R2† |
1b | 10.69 ± 0.40 | 3.31 ± 0.30‡ | 3.23 | 9.78 ± 0.37 | 1.22 ± 0.18‡ | 8.04§ |
1c | 20.61 ± 0.45 | 7.92 ± 0.76‡ | 2.6 | 22.13 ± 0.46 | 3.52 ± 0.50‡ | 6.29§ |
2a | 3.20 ± 0.16 | 1.18 ± 0.30‡ | 2.75 | 2.42 ± 0.14 | 0.37 ± 0.21‡ | 6.55 |
2b | 4.65 ± 0.19 | 1.74 ± 0.44‡ | 2.68 | 3.41 ± 0.16 | 0.53 ± 0.30‡ | 6.44 |
3b | 20.84 ± 0.37 | 6.95 ± 0.82‡ | 2.3 | 14.73 ± 0.40 | 2.92 ± 0.76‡ | 5.05 |
3c | 7.65 ± 0.30 | 1.90 ± 22‡ | 4.03 | 4.28 ± 0.30 | 0.66 ± 0.18‡ | 6.46 |
5c | 5.87 ± 0.23 | 3.23 ± 0.47‡ | 1.82 | 5.85 ± 0.22 | 2.35 ± 0.43‡ | 2.49 |
5d | 5.85 ± 0.23 | 3.22 ± 0.48‡ | 1.82 | 5.29 ± 0.22 | 2.07 ± 0.38‡ | 2.56 |
Map distances in cM (±S.E.) for intervals with and without adjacent crossovers (CO).
Ratios of map distances for intervals with and without adjacent crossovers in homozygous Col/Col (R1) and heterozygous Col/Ler (R2) backgrounds.
Significant difference in map distances in intervals when adjacent interval does or doesn't have a CO.
Significant difference between R2 and R1.
Oligonucleotides used to genotype Col-0/Ct-1 polymorphisms.