SnRK1-triggered switch of bZIP63 dimerization mediates the low-energy response in plants
- University of Vienna, Austria
- University of Würzburg, Germany
- Instituto Gulbenkian de Ciência, Portugal
- University of Tübingen, Germany
- Universidad Politécnica de Madrid, Spain
- Utrecht University, Netherlands
Abstract
Metabolic adjustment to changing environmental conditions, particularly balancing of growth and defense responses, is crucial for all organisms to survive. The evolutionary conserved AMPK/Snf1/SnRK1 kinases are well-known metabolic master regulators in the low-energy response in animals, yeast and plants. They act at two different levels: by modulating the activity of key metabolic enzymes, and by massive transcriptional reprogramming. While the first part is well established, the latter function is only partially understood in animals and not at all in plants. Here we identified the Arabidopsis transcription factor bZIP63 as key regulator of the starvation response and direct target of the SnRK1 kinase. Phosphorylation of bZIP63 by SnRK1 changed its dimerization preference, thereby affecting target gene expression and ultimately primary metabolism. A bzip63 knock-out mutant exhibited starvation-related phenotypes, which could be functionally complemented by wild type bZIP63, but not by a version harboring point mutations in the identified SnRK1 target sites.
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Author details
Reviewing Editor
- Thorsten Nürnberger, University of Tubingen, Germany
Version history
- Received: November 30, 2014
- Accepted: August 10, 2015
- Accepted Manuscript published: August 11, 2015 (version 1)
- Accepted Manuscript updated: August 18, 2015 (version 2)
- Version of Record published: September 3, 2015 (version 3)
Copyright
© 2015, Mair et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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SnRK1-triggered switch of bZIP63 dimerization mediates the low-energy response in plants
eLife 4:e05828.
https://doi.org/10.7554/eLife.05828
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