Characterization of the finch embryo supports evolutionary conservation of the naive stage of development in amniotes
- RIKEN Center for Developmental Biology, Japan
- National Center for Biological Sciences, India
Figures
Figure 1
Morphology of the finch blastoderm at oviposition.
(A–C) Finch embryos were sectioned after Hhex in situ hybridization to reveal the morphology of the blastoderm. At EGK-VI the blastoderm is thick, with no sign of epithelialization, and does not …
Figure 2
Molecular characterization of the finch blastoderm at oviposition.
(A–D) Whole-mount staining of EGK-VIII finch embryos of the distribution of putative germ cell markers SSEA-1 (A, B) and epithelial membrane antigen 1 (EMA-1) (C, D) showing views from the vitelline …
Figure 3
Expression of pluripotent markers in the finch embryo at oviposition.
(A) Comparison of the NANOG (N) and NANOG-LIKE (nl) homeodomains (HD) from chick and the finch compared to the NANOG HD from human, mouse, rat, and zebrafish, together with percentage identity to …
Figure 4
Q-PCR characterization of finch and chick blastoderm at oviposition reveals fundamental molecular differences.
Quantitative-PCR was used to assess the differences in gene expression between finch (green bars) and chick (red bars) embryos at oviposition. The markers used were associated with general …
Figure 5
Q-PCR characterization of finch blastoderms at oviposition and aged to EGK-X.
Quantitative-PCR was used to assess the differences in gene expression between finch blastoderms at laying (green bars) and aged to EGK-X, equivalent to chick oviposition (blue bars). The markers …
Figure 6
Finch blastodermal cell cultures retain markers of pluripotency even in the presence of MEK inhibitor.
Finch and chick embryos at oviposition were dissociated and cultured in the presence of LIF and the MEK inhibitor, PD0325901, for 4 days. (A–C) Finch blastodermal cells form aggregates (red arrows) …
Figure 7
Putative conservation of the naive stage during amniote embryogenesis.
Similar to mouse pre-implantation embryos (around E4), prior to epiblast epithelialisation, zebra finch embryos at oviposition are at early–mid-blastula stages (EGK-VI/VIII) show no overt …
Additional files
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Supplementary file 1
(A) Details of primers that were used for generating in situ hybridization riboprobes for the zebra finch. (B) Details of primers that were used for quantitative PCR.
- https://doi.org/10.7554/eLife.07178.010
