Certain forms of translational regulation, and translation itself, rely on long-range interactions between proteins bound to the different ends of mRNAs. A widespread assumption is that such interactions occur only in cis, between the two ends of a single transcript. However, certain translational regulatory defects of the Drosophila oskar (osk) mRNA can be rescued in trans. We proposed that inter-transcript interactions, promoted by assembly of the mRNAs in particles, allow regulatory elements to act in trans. Here we confirm predictions of that model and show that disruption of PTB-dependent particle assembly inhibits rescue in trans. Communication between transcripts is not limited to different osk mRNAs, as regulation imposed by cis-acting elements embedded in the osk mRNA spreads to gurken mRNA. We conclude that community effects exist in translational regulation.https://doi.org/10.7554/eLife.10965.001
Genes encode the instructions needed to make proteins and other molecules. To make a protein, the DNA within a gene is copied to produce molecules of messenger ribonucleic acid (mRNA) that are then used as templates to build proteins via a process called translation. This process – which involves protein machines called ribosomes binding to the start of the mRNA – is tightly regulated to control the amounts of particular proteins in cells. For example, in fruit fly ovaries, a protein called Bruno both represses and activates the translation of a gene known as oskar. To achieve this, Bruno binds to regions near the end of the oskar RNA known as Bruno response elements.
It is not clear how Bruno acts to control translation. However, because ribosomes begin translation near the start of the mRNA, while Bruno is bound to regions near the end of the mRNA, there must be long-range interactions between the two ends of the mRNA. It is generally assumed that such long-range interactions only occur between proteins that are bound to the same mRNA molecule. However, in 2010, researchers observed that Bruno response elements within one oskar mRNA could influence the translation of other oskar mRNAs. This is known as “regulation in trans”. Here, Macdonald et al. – including some of the researchers from the earlier work – investigated this observation in more detail in fruit flies.
In cells, multiple mRNA molecules and their associated proteins can assemble into particles. Macdonald et al. proposed that the close proximity of many mRNA molecules in these particles could allow trans regulation to take place. Indeed, the experiments found that blocking the assembly of oskar mRNA into particles inhibited trans regulation as expected. Macdonald et al. also asked if trans regulation can occur between mRNAs that encode different proteins. The experiments show that oskar mRNA could block the translation of an mRNA produced by the gurken gene, even when oskar mRNA was not being translated. More work is needed to find out how widely trans regulation is used to control translation.https://doi.org/10.7554/eLife.10965.002
Translation happens when a ribosome assembles productively on an mRNA. A number of molecular interactions influence the proper convergence of these parts, often involving RNA elements or factors at the opposite ends of the mRNA (Jackson et al., 2010). For example, Poly(A) binding protein (PABP) binds to the poly(A) tail at the 3’ end of an mRNA (Mangus et al., 2003) and enhances translation through its interaction with eIF4G, a protein associated with the 5’ cap of the mRNA (Tarun et al., 1997; Sonenberg and Dever, 2003). Similarly, many repressors of translation bind to elements in the 3’ untranslated regions (3’ UTRs) of mRNAs and interfere with the normal action of initiation factors at the 5’ end of the mRNA (Jackson et al., 2010; Gebauer and Hentze, 2004). Such long-range protein-protein interactions, spanning the full length of the mRNA, are generally assumed to occur only in cis: PABP bound to the tail of one molecule of mRNA is expected to bind only the eIF4G associated with the other end of the same mRNA. In principle, however, inter-transcript interactions are also possible, and their likelihood depends on the concentration of the mRNAs. When mRNAs are dilute, intra-transcript interactions will predominate, but as the local density of mRNAs increases, inter-transcript interactions become more likely.
Previously, we described an example involving the Drosophila oskar (osk) mRNA, in which control elements on one transcript influenced translation of another transcript (Reveal et al., 2010). osk mRNA is expressed during oogenesis, and is subject to multiple forms of post-transcriptional control. Following transcription in the nurse cells of each egg chamber, osk mRNA is transported through cytoplasmic connections to the transcriptionally-silent oocyte. Eventually, as oogenesis proceeds, osk mRNA localizes to the posterior pole of the oocyte, and only then does Osk protein begin to accumulate. Translation of osk mRNA is highly regulated: repression prevents premature translation from unlocalized mRNA, and activation turns on translation of the localized mRNA (Kim-Ha et al., 1995; Markussen et al., 1995; Rongo et al., 1995). Both types of regulation rely on binding sites for Bruno (Bru) (Reveal et al., 2010; Kim-Ha et al., 1995; Webster et al., 1997; Reveal et al., 2011). These sites, BREs and others, reside in two clusters - the AB and C regions - near the opposite ends of the 3’ UTR (Figure 1A). All the BREs contribute to translational repression (Kim-Ha et al., 1995), and the C region BREs also play a role in translational activation (Reveal et al., 2010). Strikingly, defects in either repression (from mutation of all BREs in osk ABC BRE-) or activation (from mutation of the C BREs in osk C BRE-) can be rescued by the presence of another osk mRNA, one that itself cannot make Osk protein but has all regulatory elements intact (Reveal et al., 2010).
To explain rescue in trans, we proposed that long-range protein-protein interactions bridging the two ends of an mRNA could also bridge two different mRNAs, to enable 'regulation in trans'. A proposed mechanism for translational repression by Bru relies on such a long-range interaction. Bru binds Cup, a protein that competes with eIF4G for binding to eIF4E, the 5’ cap-binding protein. Cup engagement of eIF4E at the expense of the eIF4E/eIF4G interaction blocks translation initiation (Nakamura et al., 2004). In our model, the Bru/Cup complex bound to the rescuing mRNA would bridge to eIF4E bound to the cap of the mRNA unable to bind Bru, thereby conferring translational repression. We hypothesized that assembly of osk mRNAs into ribonucleoprotein particles (RNPs) places the mRNAs in close proximity, thus increasing their concentration and facilitating inter-transcript interactions to allow 'regulation in trans' (Reveal et al., 2010).
If this hypothesis is correct, several features of 'regulation in trans' are expected. First, the rescuing (donor) mRNA should have the regulatory element that the rescued (recipient) mRNA lacks. Second, rescue in trans should not be Bru-specific, but should extend to other forms of regulation that also rely on long-range protein-protein interactions. Finally, rescue in trans would depend on the cis-acting osk mRNA elements and trans-acting proteins that mediate RNP assembly.
Several participants in osk RNP assembly are known - Polypyrimidine tract binding protein (PTB), Bru and an RNA dimerization motif - and all are candidates to support rescue in trans. PTB binds osk mRNA to form RNPs in vitro (Besse et al., 2009). PTB also mediates association of osk mRNAs in vivo, as shown by the effects of loss of PTB on hitchhiking (Besse et al., 2009), the phenomenon in which localization-competent osk mRNAs can assist other mRNAs in moving to the posterior pole of the oocyte (Hachet and Ephrussi, 2004). Similarly, Bru mediates in vitro oligomerization of RNAs with an unusually high density of Bru binding sites and could also oligomerize osk mRNA in vivo (Chekulaeva et al., 2006). Finally, osk mRNA undergoes direct RNA dimerization, relying on a dimerization motif in the 3’ UTR (Jambor et al., 2011). Elimination of one or more types of osk RNP assembly would be expected to block rescue in trans if our hypothesis is correct.
Here, we show that all these expectations for the 'regulation in trans' model are met. Disruption of individual contributions to RNP assembly reveals that PTB has a critical role in rescue in trans, with direct RNA dimerization being much less important. Furthermore, we provide evidence that regulation imposed by embedded cis-acting elements in osk mRNA spreads to the another mRNA bound by PTB, the gurken (grk) mRNA, thus revealing a community effect in translation.
Previously, we found that translational regulatory defects associated with mutation of Bru binding sites were rescued in trans. To determine if rescue in trans is a property unique to Bru-dependent regulation, or more general, we have now identified and tested additional regulatory mutants.
The osk gene produces two Osk isoforms, Long Osk and Short Osk, from two translation start codons (Figure 1A) (Markussen et al., 1995). The oskM1R∆121–150 mutant lacks the start codon for Long Osk and has a 30 nt deletion in the 5’ UTR for Short Osk (Figure 1A). This mutant is largely defective in Short Osk protein production, in addition to making no Long Osk because of the M1R mutation (Kanke and Macdonald, 2015). Although coexpression with osk54 (a protein null mutant with a short DNA insertion, introducing a stop codon at the end of the first exon) (Kim-Ha et al., 1991) strongly rescues the activation defect of osk C BRE- (Figure 1B), no significant rescue of the oskM1R∆121–150 activation defect was found (Figure 1B). Therefore, the oskM1R∆121–150 mutant is not sensitive to rescue in trans.
Mutations positioned close to the 3’ end of the osk mRNA also disrupt Osk expression. Mutants osk3’977–981 and osk3’984–988 (numbering indicates position in the osk 3’ UTR) have almost no osk patterning activity and are strongly defective in Osk protein production (Ryu and Macdonald, 2015). Mutant osk3’1004–1008, in which an A-rich sequence is disrupted (Kanke et al., 2015)(Figure 1A), also had strongly reduced osk patterning activity (Figure 1B). Although these mutations are positioned close to Bru-binding sites in the osk 3’ UTR C region, they have little or no effect on Bru binding (Kim-Ha et al., 1995; Ryu and Macdonald, 2015). Instead, the 977–980 and 984–988 mutations disrupt binding of BSF protein (Ryu and Macdonald, 2015), while the sequence affected by mutation 1004–1008 is a binding site for Poly(A) binding protein (PABP) (Vazquez-Pianzola et al., 2011). The patterning defects of all three of these mutants were strongly rescued by coexpression of osk54 mRNA (Figure 1B, [Ryu and Macdonald, 2015]). Similarly, a more direct assay of Osk protein expression, using an osk::GFP transgene bearing the osk3’1004–1008 mutation, showed rescue in trans (Figure 1C).
From analysis of these four additional regulatory mutants, we find a correlation (albeit with few examples) between the type of regulatory defect and its ability to be rescued in trans: regulatory elements likely to engage in long-range interactions (i.e. those in the 3’ UTR) are sensitive to rescue in trans, while a regulatory element positioned near the site of translational initiation and thus less likely to participate in a long-range interaction is not. Notably, by the model of 'regulation in trans' only the former type of regulatory element should be sensitive to rescue in trans, consistent with the results.
Thus far, osk54 has been used as the donor for rescue in trans of osk mRNA regulatory defects. To determine if the ability to rescue is typical, or is an unusual property of osk54, additional osk mutants unable to provide Osk patterning activity were tested. In oskM139L, the translation start codon for the Short Osk protein isoform is mutated. Because Short Osk is the isoform with embryonic patterning activity, oskM139L lacks that activity (Vanzo and Ephrussi, 2002). Like osk54, oskM139L strongly rescued the translational activation defect of osk C BRE- and restored normal embryonic patterning (Figure 2A). Likewise, oskM1R∆121–150, the mutant whose regulatory defect is not rescued in trans, was an effective donor for rescue of the osk C BRE- mutant in trans (Figure 2A). Rescue was also assayed by examination of Osk protein production, using the osk::GFP C BRE- mRNA as the recipient and monitoring GFP fluorescence (this allows the mRNA producing the protein to be unambiguously identified, as the donor mRNAs do not include GFP). Both oskM139L and oskM1R∆121–150 restored Osk::GFP protein expression to wild-type levels, just as with the osk54 donor (Figure 2C).
These results, and others (below), show that a variety of osk mutants can act as donors for rescue in trans; this property is not unique to osk54. Furthermore, rescue by oskM1R∆121–150 is consistent with 'regulation in trans', as the oskM1R∆121–150 mRNA includes the regulatory element that the osk::GFP C BRE- mRNA lacks.
The model for 'regulation in trans' predicts that the regulatory element missing from the recipient mRNA must be provided by the donor. This idea can be tested with osk::GFP C BRE- as the recipient, and osk C BRE- as the donor, as both carry the same regulatory mutation. Although the activation defect of osk::GFP C BRE- was strongly rescued by osk54 (and by other donors with intact C region BREs; Figure 2C, below), little or no GFP signal was detected with osk C BRE- as the donor (Figure 2C).
Having shown that rescue of a regulatory defect in the recipient mRNA requires the cognate, wild type regulatory element in the donor mRNA, we next asked if the presence of that element was sufficient for rescue, or if other sequences (e.g. those that might contribute to RNP assembly) were also required. Portions of the osk mRNA 3’ UTR were added to a UAS-GFP transgene, and expressed using the UAS/GAL4 system (Brand and Perrimon, 1993). The osk 3’ UTR AB and C regions contain the 5’ and 3’ clusters of Bru binding sites, respectively (Figure 1A). These regions were tested alone, or in combination. Also tested was the entire osk 3’ UTR.
The UAS-GFP donor did not rescue the activation defect of osk C BRE- (Figure 2D, compare to Figure 2A). Similarly, the donor with the osk 3’ UTR AB region provided no rescue. A donor carrying the osk 3’ UTR C region, and thus the activation element mutated in the osk C BRE- recipient, provided a low level of rescue. Rescue was increased when both the AB and C regions were present, and the highest level of rescue was obtained when the GFP mRNA included the entire osk 3’ UTR. The different levels of rescue cannot be attributed to differences in the amounts of the various donor mRNAs (Figure 2E).
The results of these experiments are consistent with 'regulation in trans'. The C region must be present to provide the missing translational activation element, while other Bru binding sites that do not contribute to activation (the AB region sites) are not sufficient. The weak rescue from the C region alone is consistent with the notion that rescue requires both the missing element and sequences which mediate assembly of osk RNPs. We suggest that by itself the C region supports weak association of GFP-C mRNAs with osk C BRE- transcripts, and this association is strengthened moderately by addition of the AB region and more strongly by addition of the full 3’ UTR.
To ask if RNP assembly is required for rescue in trans, we wished to inhibit or abolish each of the known forms of RNP assembly. Removing assembly factors - PTB and Bru - is not a good approach, as these proteins bind to many mRNAs and indirect effects on osk mRNA regulation are possible. In a more selective approach, the osk mRNA is modified, mutating elements involved in RNP assembly. With donor osk transgenes defective in one or more types of RNP assembly, any change in the ability to rescue the activation defect of the osk::GFP C BRE- recipient mRNA can be monitored.
Testing the role of Bru-mediated osk mRNA oligomerization (Chekulaeva et al., 2006) is problematic, as the Bru sites are also required to provide the missing regulatory element for osk C BRE- (above). However, the other contributions to assembly - mRNA dimerization and PTB-dependent RNP assembly - can be manipulated (Figure 3A). Direct osk mRNA dimerization requires a RNA element (Jambor et al., 2011), which is deleted in mutant osk3’∆665–685 (labeled as dimer- in Figure 3). Testing the role of PTB-mediated assembly could be done by mutating PTB-binding sites. These sites are spread throughout the osk mRNA 3’ UTR (as shown by RNA binding assays), although individual sites have not been defined (Besse et al., 2009). However, because the binding specificity of Drosophila PTB is likely to be very similar or identical to that of the highly conserved mammalian homolog, for which binding sites have been characterized by a combination of approaches (Pérez et al., 1997; Xue et al., 2009), candidate sites can be identified (Figure 3—figure supplement 1). Mutating all the candidate sites (consisting of pyrimidine tracts or PTs) is the simplest strategy to disrupt PTB binding, but introducing so many mutations increases the probability of an inadvertent effect on rescue in trans. To strike a balance between these design considerations, a subset of the PT sequences was mutated (osk3’PTmut)(Figure 3—figure supplement 1), focusing on those with features associated with the strongest sites for mammalian PTB binding, while avoiding mutations that might also disrupt osk mRNA dimerization or Bru binding. A UV crosslinking competition binding assay was used to determine if the PT mutations affected PTB binding (Figure 3B). PTB bound the radiolabeled osk mRNA 3’ UTR probe mRNA (left lane). Increasing amounts of unlabeled osk 3’ UTR RNA (osk+) effectively competed for binding. When the competitor RNA included the PT mutations (3’PTmut), competition was less effective, demonstrating that the mutations do affect PTB-binding sites.
We coexpressed different donor mRNAs (all present at similar levels; Figure 3D) together with the activation-defective osk::GFP C BRE- recipient mRNA, and measured posterior GFP levels in late stage oocytes to determine how effectively the activation defect was rescued (Figure 3C). The wild-type donor provides strong rescue of activation, as does the donor lacking the dimerization motif (dimer-)(Figure 3E). By contrast, the osk3’PTmut and osk3’PTmut dimer- donors provided almost no rescue of activation. Although no statistically significant difference between GFP levels with these latter two donors was detected, some oocytes had trace levels with the osk3’PTmut donor, while none were above background with the osk3’PTmut dimer- donor, consistent with an additive effect of the two types of mutations (Figure 3E). Examples of the trace posterior GFP signals with the osk3’PTmut donor, and their absence with the osk3’PTmut dimer- donor, are shown in Figure 3—figure supplement 4. The loss of rescue in trans cannot be attributed to failure of the donor mRNAs to localize to the posterior pole of the oocyte, as the PT mutations do not interfere with posterior localization (Figure 3—figure supplement 5).
Mutation of candidate PTB-binding sites in the osk mRNA clearly disrupts rescue in trans. Loss of PTB-dependent RNP assembly is one explanation of the results, and is consistent with 'regulation in trans'. Alternatively, inadvertent mutation of the binding site of some other factor could make the osk3’PTmut mRNA a poor donor. To distinguish between these options two approaches were used.
For one approach, we reasoned that if osk3’PTmut failed to rescue because of reduced PTB binding, this would likely be an additive defect that depends on mutation of multiple different PTs. Thus, mutating only subsets of the PTs might yield intermediate levels of rescue. By contrast, if loss of rescue was due to inadvertent mutation of a binding site for another factor, then, assuming that this other factor does not have the same binding specificity as PTB, this effect would likely be due to a specific PT mutation. If so, we would expect that only a single subset of PT mutations would disrupt rescue in trans.
The mutations in osk3’PTmut were subdivided into four groups (Figure 4A, Figure 3—figure supplement 1). To confirm that each subset of PT mutations reduced PTB binding, short segments of the osk mRNA 3’ UTR containing the subsets of mutations were tested in UV crosslinking assays. Each of the four 3’ UTR segments bound PTB, and for each the binding was substantially lower when the RNA contained the PT mutations (Figure 4B).
The same subsets of mutations were also introduced into osk donor transgenes (Figure 4A, Figure 3—figure supplement 1). Just as for the osk3’PTmut mRNA, all the new osk donor transgenes were properly localized to the posterior pole of the oocyte (Figure 3—figure supplement 5). Each of these donors was impaired for rescue of the translational activation defect of the osk::GFP C BRE- recipient, although none was as defective as the osk3’PTmut donor (Figure 4D). Thus, the PT mutations had an additive effect, and some subsets of the mutations inhibited rescue in trans more than others. The differences in rescuing activity did not correlate with the amounts of the different donor mRNAs (Figure 4E). To determine if reduced donor activity displayed a correlation with reduced PTB binding activity, PTB binding was measured in competition-binding assays (Figure 4C). Binding of PTB to a radiolabeled wild type osk 3’ UTR RNA was performed in the presence of increasing amounts of unlabeled osk 3’ UTR competitor RNAs. Competitors with subsets of PT mutations had intermediate levels of competition, not as strong as the wild type osk 3’ UTR but stronger than the osk 3’ UTR with all PT mutations. Notably, among this group, the osk 3’ UTR RNA with the 3’375–540 subset of PT mutations was the weakest competitor, just as the osk3’375–540 PTmut transgene was the least effective donor for rescue in trans. Thus, we did observe a correlation between strength of PTB binding and strength of rescue in trans. For the other three subsets of PT mutations, their effects on PTB binding were too similar to one another to draw clear distinctions among them (Figure 4C), much like the effects of these mutations on rescue in trans (Figure 4D).
As a second approach to ask if PTB is required for rescue in trans, no PTB binding sites in either donor or recipient osk mRNAs were mutated, but instead the level of PTB protein was reduced. The osk+ donor and osk::GFP C BRE- recipient mRNAs were coexpressed in flies either wild type or heterozygous mutant for heph (heph encodes PTB)(Figure 4F, right). Reducing the level of PTB activity in the heph heterozygotes impaired rescue of the translation activation defect, causing a lower level of Osk::GFP. By contrast, in a control experiment using a recipient mRNA with no translation defect, osk::GFP, reducing the level of PTB activity had no effect on Osk::GFP levels (Figure 4F, left).
Thus, the results of both kinds of tests to determine the role of PTB - mutating candidate PTB-binding sites or reducing PTB activity - point to the same conclusion: PTB-dependent assembly of osk mRNA into RNPs is required for rescue in trans, supporting the model of 'regulation in trans'.
If assembly of osk transcripts in RNPs promotes 'regulation in trans', would translation of other mRNAs present in the same RNPs also be influenced by the translational control elements in the osk mRNA? Candidates for such trans regulation are expected to share two features with osk mRNA: reliance on a shared factor for RNP assembly, and overlap in subcellular distribution. Because PTB is most strongly implicated in rescue of osk mRNA regulatory defects in trans, we focused on mRNAs with candidate PTB-binding sites.
Among ovarian mRNAs, one of the most highly enriched for candidate PTB-binding sites is gurken (grk) (Supplementary file 1). Furthermore, PTB associates with grk mRNA, both in vitro and in vivo (McDermott et al., 2012; McDermott and Davis, 2013). Notably, grk mRNA is, like osk, highly enriched in the oocyte during previtellogenic stages (Figure 5A,B) (Neuman-Silberberg and Schüpbach, 1993). Later, at stage 8, both mRNAs align at the anterior margin of the oocyte, but then go their separate ways within the oocyte: grk becomes restricted to the anterodorsal corner, while osk moves to the posterior (Kim-Ha et al., 1991; Neuman-Silberberg and Schüpbach, 1993; Ephrussi et al., 1991). Thus, grk and osk mRNAs have similar distributions for part of oogenesis and have the potential to reside in the same RNPs during that period. Consequently, each mRNA could influence the translation of the other. Because translation of osk mRNA is strongly repressed for the entire period of shared localization, the most likely trans effect is for osk mRNA to confer some degree of translational repression on grk mRNA.
To address the possibility that osk transcripts influence grk mRNA translation, we monitored Grk protein levels in the oocytes of previtellogenic stage 5/6 egg chambers (Figure 5C). If wild-type osk mRNAs are present (Figure 5D upper), then 'regulation in trans' is possible. If osk3’ PTmut mRNA is the only osk mRNA present (Figure 5D lower; loss of PTs is indicated by fewer yellow dashes), then any PTB-dependent association of osk and grk mRNAs should be impaired, and 'regulation in trans' could be reduced. Remarkably, the type of osk mRNA present did affect Grk protein levels. When only osk3’PTmut mRNA was present, Grk protein levels were higher than when osk+ mRNA was also present (Figure 5I). This effect was observed in each of two separate experiments, making use of different lines of the osk3’PTmut transgene. As a control, we performed the same type of experiment with an osk transgene that is a strong donor for rescue of osk mRNA regulatory defects and found no change in Grk protein levels (Figure 5H). The differing behaviors of the osk3’PTmut and osk control transgenes cannot be attributed to differences in mRNA levels (Figure 5G). Importantly, none of the osk mRNAs in these experiments produce any Osk protein at these stages of oogenesis (Figure 5E,F). Therefore, we conclude that osk mRNA, not Osk protein, influences Grk expression. The increase in Grk levels when osk mRNA is impaired in PTB-dependent RNP assembly is consistent with the strong translational repression of osk mRNAs acting in trans to confer some degree of translational repression on grk mRNAs present in the same RNPs.
As described above, we found that each of several osk mutants with defects in 3’ UTR regulatory elements could be rescued in trans, consistent with the notion that rescue relies on long-range interactions that bridge between donor and recipient mRNAs. However, the osk IBE-mutant is an exception to this rule. The IBE sequence - UUUAY (Y is pyrimidine) - was identified as a binding site for Imp protein (Munro et al., 2006). Thirteen copies of the IBE appear in the osk mRNA, all in the 3' UTR. The osk IBEs were mutated in subsets to test their role. Three of the mutants (subsets A, C and D; Figure 6C) fail to accumulate Osk despite having normal localization of osk mRNA, while the fourth mutant (subset B) has normal Osk expression. Mutating the A, C, or D subsets of IBEs disrupts colocalization of Imp protein with osk mRNA at the posterior pole of stage 9/10 oocytes, supporting the notion that Imp binds osk mRNA through these elements and mediates activation of osk mRNA translation (Munro et al., 2006). However, mutants lacking Imp protein have normal osk expression, suggesting that the IBEs also bind another factor, with this second factor contributing to activation of osk translation (Munro et al., 2006; Geng and Macdonald, 2006).
Previously, we found that the osk IBE- mutant was not strongly rescued in trans (Reveal et al., 2010). Now, we asked if the osk IBE- mutant can act as a donor for rescue in trans. Neither of two transgenes with mutated IBEs strongly rescued the translational activation defect of the osk C BRE-mutant (Figure 6A). Similarly, neither IBE mutant restored protein expression of the osk::GFP C BRE- mutant (Figure 6F, data not shown). Failure to rescue strongly was not due to insufficient mRNA, as both IBE mutant osk mRNAs were present at high levels (Figure 6B). Thus, mutation of the IBEs disrupted rescue in trans, with the mutant osk mRNAs unable to act as either donor or recipient.
Several features of the IBE mutants, notably their inability to participate in rescue in trans, as well as a report that Imp protein has only weak affinity for osk mRNA (Geng and Macdonald, 2006), prompted further analysis of the IBEs. We made two new mutants (Figure 6D,E), each affecting a subset of IBEs implicated in activation of osk translation (Munro et al., 2006). In mutant osk 11-13-, the 3’-most IBEs (subset D) were altered. Instead of mutating the central position in the UUUAY pentamer from U to G (as in the original osk IBE-mutants), each nucleotide of each pentamer was changed, and so a stronger defect in binding - to Imp or to the unknown factor - is expected. Notably, the osk 11-13- mutant had normal Osk accumulation (Figure 6D). For mutant osk 3'∆83–116, the entire region encompassing the 5’-most IBEs (subset A) was deleted. Again, this mutant is expected to more strongly disrupt Imp/unknown binding than the corresponding osk IBE- mutant, but it had normal Osk accumulation (Figure 6E). Thus, the original IBE mutations appear to disrupt translational activation of osk mRNA not by removing a binding site, but by creating a novel type of element, such as a binding site for another factor.
The new mutants lacking subsets of IBEs were also tested for the ability to act as donors for rescue in trans. The activation-defective osk:GFP C BRE- was used as the recipient, and we monitored GFP to determine if activation was rescued. The original osk IBE- mutant did not substantially rescue translation of the recipient mRNA. By contrast, both osk 3'∆83–116 and osk 11-13- mutants strongly rescued translational activation (Figure 6F). It seems likely, then, that the gain-of-function property of the original IBE- mutations not only disrupts osk mRNA translation, but also interferes with the ability of the mRNA to participate in rescue in trans.
Long-range interactions involving structures or factors associated with the different ends of an individual mRNA are required for translation. Such interactions are not inherently intra-transcript, and a factor bound to the 5’ end of one mRNA could engage a factor bound to a 3’ region of another mRNA. Nevertheless, inter-transcript interactions have generally not been considered in models of translation or its regulation. The osk mRNA is an exception, displaying the notable property of rescue of regulatory mutations in trans. We suggested that this phenomenon results from inter-transcript interactions, with the participating mRNAs placed in close proximity in RNPs to facilitate the interactions. The work described here establishes parameters for this rescue in trans, shows that conditions which reduce RNP assembly diminish rescue in trans and provides evidence that regulation imposed on the osk mRNA influences the expression of grk mRNA by a similar trans effect. Our results are fully consistent with and lend considerable support to the model of 'regulation in trans'.
We have now asked which regulatory defects in the recipient mRNAs can be rescued in trans, and what features are required in the donor mRNAs to allow rescue. Learning more about the underlying rules can provide support for, or argue against, the model of 'regulation in trans'.
We first consider the recipient mRNAs. In the context of the proposed model, mutations affecting long-range regulatory interactions should be susceptible to rescue in trans, while other types of regulatory defects would not. Testing new regulatory mutants as recipients, we found that three mutants with mutations in the 3’ UTR could be rescued in trans, while a mutant with defects in the 5’ region of the mRNA could not. Sensitivity of the 3’ UTR regulatory elements to rescue in trans is consistent with their participation in long-range interactions to activate translation. In particular, the osk3’1004–1008 mutant is defective in an A-rich element, which likely serves to bind PABP (Vazquez-Pianzola et al., 2011). PABP interacts with the translation initiation factor eIF4G (Tarun et al., 1997), associated with the mRNA 5’ cap, and this interaction has the potential to occur between different transcripts. As for the 5’ region mutant which is not rescued in trans, why activation of translation is defective is not understood (Kanke and Macdonald, 2015), but the position of the mutations suggests a local, rather than long-range, effect on initiation of translation.
Experiments addressing the requirements for an mRNA to act as a donor in rescue in trans lead to several conclusions. First, we find that the element missing from the recipient must be provided by the donor, as predicted by the model of 'regulation in trans' [but also consistent with other models - see (Wilusz and Wilusz, 2010)]. Second, although the missing element is required in the donor, it is not sufficient, and additional 3’ UTR sequences must be present to obtain a substantial degree of rescue. These results are also consistent with 'regulation in trans', with the additional sequences presumably acting to promote interactions between the different transcripts and thus allow the missing regulatory element to act in trans. Third, many osk mRNAs can act as donors for rescue in trans; this appears to be the default property. Even if an osk mRNA is not receptive to rescue in trans, this does not exclude the possibility that it can act as a donor. For example, the oskM1R∆121–150 mutant cannot be rescued in trans but is a strong donor. Again, these observations are consistent with 'regulation in trans', in which the donor mRNA need not itself be translated, it needs only to contain the regulatory element missing from the recipient as well as sequences that mediate association of the different mRNAs. Fourth, there are mutations which interfere with the ability of an osk mRNA to act as a donor for rescue in trans. These fall into two groups, the enigmatic IBE mutant, and the mutants designed to disrupt RNP assembly.
The IBE mutant is completely defective in activation of osk mRNA translation (Munro et al., 2006) and cannot be rescued in trans. As a donor, the IBE mutant confers only very weak rescue of other regulatory defects. New mutants targeting the IBEs, which more substantially alter or eliminate the IBE motifs, have no regulatory defects and are strong donors for rescue in trans. Thus, the original IBE mutations appear to cause a dominant gain-of-function defect, perhaps by serendipitous formation of a binding site for an unknown factor. Why do the original IBE mutations in one subset of IBE motifs, the B group, have no consequences? Notably, the B group IBEs are embedded in a cluster of Bru-binding sites. We suggest that bound Bru could occlude binding of the unknown factor, thus avoiding the adverse effects. In any event, the original IBE mutants are clearly unusual and need to be considered separately from mutants which disrupt bona fide regulatory elements. Learning how the IBE mutant inhibits rescue in trans may well provide important insights into the mechanism, and will be the focus of future work.
Finally, mutants designed to disrupt RNP assembly also fail to rescue in trans. Mutation of many PTs in the donor mRNA most dramatically disrupted rescue in trans, with lesser defects from mutating subsets of these PTs. The additive nature of the PT mutations is fully consistent with the notion that they serve as binding sites for PTB, which assembles osk mRNA into RNPs in vitro and is required for association of osk mRNAs in vivo (Besse et al., 2009). As an independent test of the role of PTB in rescue in trans, we also monitored the effects of reducing PTB gene dosage when the donor and recipient osk mRNAs had intact PTB-binding sites. Consistent with 'regulation in trans', lowering the activity of PTB diminished rescue of the translational activation defect, leading to a lower level of Osk protein. PTB is implicated in translational repression of osk mRNA (Besse et al., 2009), and so a reduced level of PTB could also influence Osk protein levels more directly. However, a lower efficiency of repression would increase, not lower, the level of Osk protein, and the effect we observe is more reasonably attributed to 'regulation in trans'.
By the model of 'regulation in trans', almost any mRNA should be susceptible to inter-transcript interactions influencing its translation. In practice, such effects would be most likely for mRNAs in close proximity to one another, either because of an overall high mRNA concentration or because of coassembly in RNPs. To consider this possibility, we focused on the grk mRNA, which shares with osk mRNA patterns of subcellular distribution as well as association with PTB. Remarkably, removing candidate PTB-binding sites from osk mRNA led to elevation of Grk protein levels early in oogenesis. Because translation of osk mRNA is strongly repressed at this developmental stage, association of the osk and grk mRNAs could lead to some degree of translational repression of grk. Disrupting this association would reduce repression of grk, leading to higher Grk protein levels, as we observed. It is also possible that reducing the amount of PTB bound to osk mRNA would increase the pool of PTB available for binding to grk. However, if this occurs, given the evidence that PTB acts in the cytoplasm as a repressor of translation (Besse et al., 2009), the expected outcome would be enhanced repression of grk mRNA and less Grk protein, not more.
Our evidence that translational regulation can occur in trans, with inter-transcript interactions affecting the translation of participating mRNAs, raises the question of whether this phenomenon is unusual, or more widespread but unappreciated. Unless a mechanism exists to specifically exclude inter-transcription interactions, there is no compelling reason why such interactions would not occur, and their probability may be dictated by mRNA concentrations. The absence of other reports of this phenomenon may be misleading, as detecting trans effects is not straightforward. For osk mRNA, rescue in trans was discovered by comparing the effects of cis regulatory mutations in the presence or absence of other osk transcripts (Reveal et al., 2010). To the best of our knowledge, this approach is not common. Furthermore, it requires an RNA null allele for the regulated gene, a reagent not always available. Despite the absence of direct tests for rescue in trans, recent advances in understanding how mRNAs are localized may be relevant. Often, localized mRNAs are subject to translational regulation to ensure that the protein is only synthesized at the appropriate time or location (Jung et al., 2014). Curiously, several such mRNAs - but not osk - are transported in RNPs containing a single mRNA molecule (Batish et al., 2012; Amrute-Nayak and Bullock, 2012; Mikl et al., 2011; Little et al., 2015). Perhaps, such isolation of individual mRNAs serves to ensure that 'regulation in trans' does not occur and that regulation is constrained to that imposed by embedded cis elements.
Mutants at the osk locus: osk54 (Lehmann and Nüsslein-Volhard, 1986), oskA87 (Jenny et al., 2006), Df(3R)osk (Reveal et al., 2010), and osk0 (Kanke et al., 2015). For experiments in which an osk transgene was tested as the only source of osk mRNA, the background was an osk RNA null combination, either oskA87/Df(3R)osk, oskA87/osk0, osk0/Df(3R)osk, or osk0/osk0. We have not observed any differences in phenotypes from use of the different osk RNA null combinations. Genomic osk transgenes were based on a genomic fragment that fully rescues osk null mutants (Kim-Ha et al., 1991). The genomic osk::GFP transgenes include the complete osk sequence, with mGFP6 (Haseloff, 1999) introduced at position T140 (one codon after the initiation codon for Short Osk). Except for osk IBE- (Munro et al., 2006) and osk C BRE- (Reveal et al., 2010), all genomic osk transgenes lacking GFP have three copies of the HA epitope tag at position T140; this tag has no detectable effect on osk activity (Kanke and Macdonald, 2015; Jones and Macdonald, 2015; Kim et al., 2015). IBE- transgenes have the A subset (Munro et al., 2006) of IBE mutations. Mutations were introduced by PCR, or from synthetic DNA fragments (gBlocks; Integrated DNA Technologies, Coralville, Iowa). UAS-GFP transgenes, with or without parts of the osk 3’ UTR, are those used previously (Reveal et al., 2010), or are identical but differ in the portions of the osk 3’ UTR included. The matalpha4-GAL-VP16 driver (Martin and St Johnston, 2003) was used for expression of UAS-GFP transgenes.
Female flies of the genotype to be tested, together with sibling males, were aged in well-yeasted vials for 3–4 days after eclosion, and then transferred to small population cages for egg laying on yeasted apple juice plates. The plates were changed at intervals (~12–24 hr), the embryos aged for a further 20–24 hr, and cuticles were prepared for mounting in Hoyer’s medium (Wieschaus et al., 1986). A wild-type embryo has eight abdominal ventral denticle belts. When osk activity is reduced, fewer denticle belts form with the number of belts proportional to the level of osk activity. Conversely, if there is excess osk activity, anterior structures are lost. For the transgenes studied here, only reduced osk activity was detected. Each embryo was scored for the number of complete abdominal denticle belts. The results presented in Figs. 1B, 2A and 6A represent the embryos from three or more sequential embryo collections. For the results of Figure 2D, the results from two separate experiments (i.e. independent sets of crosses to generate the flies) were combined to increase sample sizes.
RNA levels were determined by RNase protection assays, using rp49 for normalization of sample amounts (Reveal et al., 2010). At least three assays were done for each mRNA. RNA distributions were detected by in situ hybridization (Jambor et al., 2011).
Proteins were imaged in whole mount samples by confocal microscopy with a Leica TCS-SP (Reveal et al., 2010; Kim-Ha et al., 1995). Antibodies for immunodetection: rabbit anti-Osk (Reveal et al., 2010), 1/3000; mouse anti-HA (Covance), 1/300; mouse anti-Grk 1D12 [Developmental Studies Hybridoma Bank (DSHB)], 1/10; and mouse anti-Hts 1B1 (DSHB), 1/1. TO-PRO-3 Iodide (Invitrogen)(1:1,000) was used to stain nuclei. For immunodetection, and for detection of GFP from fusion proteins, samples in comparison groups (corresponding to sets of data in outlined boxes in Figures 3–5) were from flies grown in parallel and aged in well-yeasted vials. They were dissected, fixed, and stained in parallel, and imaged in a single session using identical confocal settings in which the fluorescence signals were not saturated.
For quantitation of posterior Osk::GFP in late stage oocytes, we first determined how reliably an optical section could be visually identified as having the maximum signal intensity. Note that all late stage oocytes, having a high anterior/posterior to dorsal/ventral axial ratio, are positioned with the anterior/posterior axis parallel to the plane of the slide. This orientation ensures that a z series of images is comparable for all oocytes. A z position appearing to have the highest level of Osk::GFP signal was noted, and then a z series of images roughly centered on that position was collected. Signal intensities were determined (as described below), and plotted across the z dimension. As shown in the two oocyte examples in Figure 3—figure supplement 2, this approach was adequate to identify a z position having a signal intensity within 2% of the maximum measured intensity. To measure signal intensity of the posterior crescent of Osk::GFP, the posterior peripheral region of each oocyte was traced in FIJI using a Wacom Intuos tablet. Because oocytes differ in shape, a single standardized trace outline could not be used. When the Osk::GFP signal intensity was strong, the region to be traced was readily identified, with the boundaries of the traces extending well beyond the anterior-most signal. When Osk::GFP signal intensity was low or undetectable, a region similar in shape and size was traced. Within the traced region for each oocyte, the total signal was measured. Other options for quantitation of the posterior crescent of Osk::GFP are to determine average or maximum signal intensity. Neither provides a reliable estimate of total Osk::GFP. For an average intensity to be reliable, the area scored must be identical for all oocytes. Given the variation in oocyte shape, tracing areas identical in size is not practical. For a maximum signal intensity to be reliable, there must be a point source of Osk::GFP. This is not the case. To evaluate the consistency of the assay, a single biological sample was divided into two portion, and each was mounted on a separate slide. Both slides were imaged in a single session. Quantitation using the approach described showed a high level of consistency (Figure 3—figure supplement 3).
Quantitation of Grk protein in early oocytes presents different challenges and a different approach was used. Within early oocytes Grk protein is roughly uniform in distribution, except that the protein is excluded from the nucleus. Because all samples in these experiments contain readily detectable Grk, the position of the nucleus (unstained) is obvious and the cytoplasm can be easily traced. Given the uniform distribution of Grk in the cytoplasm, measuring an average signal intensity is appropriate. By contrast, measuring the total amount of signal does not provide a reliable estimate of Grk levels, as the area of the oocyte varies substantially depending on z position. For these images, additional steps were taken to obtain similar data from all samples. First, only egg chambers oriented in a roughly horizontal position were analyzed. This ensures that the position of the nucleus (lacking Grk), and the region of the oocyte it occupies, are similar for all samples. Second, the focal plane was chosen to include the oocyte nucleus, thus avoiding the peripheral regions of the oocyte. Near the periphery, only a small portion of the oocyte is included in an optical section, which causes high variability in average signal intensity. Limiting the analysis to the more central region yielded only minor variation in measured signal intensities (Figure 5—figure supplement 1), much less than the differences observed in the experiments of Figure 5.
A maltose-binding protein-PTB fusion protein (Besse et al., 2009) was expressed in E. coli BL21/pLysS and affinity purified on amylose resin (New England Biolabs) according to the manufacturers protocol. UV crosslinking assays were performed as described (Macdonald et al., 1995). Radiolabeled RNAs were synthesized by in vitro transcription (MaxiScript; Ambion) with alpha 32-P UTP from plasmid templates containing either the full osk mRNA 3’ UTR (Figure 3 and Figure 4C), or from plasmid templates containing short segments of the osk 3’ UTR (Figure 4B). Competition-binding experiments were performed as described (Macdonald et al., 1995). For all assays, phosphorimaging of dried gels was used to visualize and quantitate binding. All assays were performed at least three times, with no significant variation in results.
The patterning activity and imaging assays can be divided into two groups: those with dramatically different outcomes and those with graded differences which can be small. For the first group, such as the patterning assays in Figure 2A and the imaging results in Figure 2C, no statistical analysis is included. For the second group, statistical tests were used to evaluate the significance of any observed differences. For comparisons between two samples, the student’s t test was used, with p values provided in each figure. Determinations of effect size and power (post hoc calculation) were performed using G*Power (Faul et al., 2007; Faul et al., 2009). To facilitate comparisons in patterning assays of Figure 2D, embryos in different phenotypic classes were assigned numbers of abdominal denticle bands as follows: 6–7, 6.5; 3–5, 4; and 0–2, 1 (embryos scored with 8 bands retained the 8 value). The assigned values were used to calculate average number of denticles bands and standard deviations, from which effect size and power were determined in G*Power. These values were also used in student’s t tests.
Targeted gene expression as a means of altering cell fates and generating dominant phenotypesDevelopment 118:401–415.
G*Power 3: a flexible statistical power analysis program for the social, behavioral, and biomedical sciencesBehavior Research Methods 39:175–191.https://doi.org/10.3758/bf03193146
Statistical power analyses using G*Power 3.1: tests for correlation and regression analysesBehavior Research Methods 41:1149–1160.https://doi.org/10.3758/BRM.41.4.1149
Imp associates with squid and Hrp48 and contributes to localized expression of gurken in the oocyteMolecular and Cellular Biology 26:9508–9516.https://doi.org/10.1128/MCB.01136-06
GFP variants for multispectral imaging of living cellsMethods in Cell Biology 58:139–151.https://doi.org/10.1016/S0091-679X(08)61953-6
The mechanism of eukaryotic translation initiation and principles of its regulationNature Reviews. Molecular Cell Biology 11:113–127.https://doi.org/10.1038/nrm2838
Independent and coordinate trafficking of single Drosophila germ plasm mRNAsNature Cell Biology 17:558–568.https://doi.org/10.1038/ncb3143
exl protein specifically binds BLE1, a bicoid mRNA localization element, and is required for one phase of its activityProceedings of the National Academy of Sciences of the United States of America 92:10787–10791.https://doi.org/10.1073/pnas.92.23.10787
Translational control of oskar generates short OSK, the isoform that induces pole plasma assemblyDevelopment 121:3723–3732.
A repeated IMP-binding motif controls oskar mRNA translation and anchoring independently of Drosophila melanogaster IMPThe Journal of Cell Biology 172:577–588.https://doi.org/10.1083/jcb.200510044
Mutation of PTB binding sites causes misregulation of alternative 3' splice site selection in vivoRNA 3:764–778.
Localization of oskar RNA regulates oskar translation and requires Oskar proteinDevelopment 121:2737–2746.
Eukaryotic translation initiation factors and regulatorsCurrent Opinion in Structural Biology 13:56–63.https://doi.org/10.1016/s0959-440x(03)00009-5
Translation initiation factor eIF4G mediates in vitro poly(A) tail-dependent translationProceedings of the National Academy of Sciences of the United States of America 94:9046–9051.https://doi.org/10.1073/pnas.94.17.9046
Oskar anchoring restricts pole plasm formation to the posterior of the Drosophila oocyteDevelopment 129:3705–3714.
Translational repressor bruno plays multiple roles in development and is widely conservedGenes & Development 11:2510–2521.https://doi.org/10.1101/gad.11.19.2510
Looking at embryosIn: D. B Roberts, editors. Drosophila - a Practical Approach. Washington, D.C: IRL Press. pp. 199–227.
Rachel GreenReviewing Editor; Johns Hopkins School of Medicine, United States
In the interests of transparency, eLife includes the editorial decision letter and accompanying author responses. A lightly edited version of the letter sent to the authors after peer review is shown, indicating the most substantive concerns; minor comments are not usually included.
Thank you for submitting your work entitled "Community effects in regulation of translation" for consideration by eLife. Your article has been reviewed by two peer reviewers, and the evaluation has been overseen by a Reviewing Editor and K VijayRaghavan as the Senior Editor.
The reviewers have discussed the reviews with one another and the Reviewing editor has drafted this decision to help you prepare a revised submission.
Both reviews on the manuscript have been completed and there is general concordance on several points. First, both reviewers feel that the work is of broad interest in taking earlier observations by your group showing that BREs' can be rescued in trans by another mRNA transcript, and extending these observations to show that other osk mutations can also be rescued in a manner potentially dependent on the PTB protein (and its binding sites). And, while both reviewers are inclined to think that indeed this idea is correct (if not the complete story), both reviewers feel that additional experiments are needed to support this specific claim. Most importantly, the distribution of the mutant RNA needs better characterization in the presence and absence of PTB (i.e. in the presence of PTB binding site mutations and with diminished PTB levels). One of the reviewers proposes the use of FISH to help support the proposed mechanism – two color FISH should help correlate the observed regulatory effects with colocalization. The second reviewer proposes some IP style experiments to address a similar point (where the amount of RNAs recovered correlate with the predicted strengths of the PTB binding sites, etc.). There were other experiments proposed to ask whether the nature of the mRNA interaction was reciprocal, and whether co-regulation happens in both directions. The reviewers agree that this would be interesting, but perhaps beyond the scope of the present work.
In light of the broad agreement of the reviewers, we would be happy to receive a revised manuscript should there be additional experiments provided to support the proposed model that PTB binding is critical for both colocalization and coregulation, demonstrated more directly through biochemistry/visualization, and not simply through genetic approaches.
This manuscript is a follow up of the previous 2010 Developmental Cell paper (Dual Functions of BREs in Translational Regulation), the authors found that mutation of osk 3' UTR Bruno Recognition Element (BRE) results in developmental defects (measured in the number of cuticles in embryos). Coexpression of a second, protein null osk transcript (osk54) is able to rescue the developmental defects.
The authors carried out more mutations in the donor and the recipient osk mRNAs to try to understand what factors/elements are important for the rescue in trans. The authors characterized developmental defects and used GFP reporters to monitor rescue. There are two new conclusions from the paper. (1) PTB dependent mRNP assembly is essential for rescue in trans. (2) The translation regulation can affect other RNAs in the same RNP. Both of these are consistent with their in trans regulation model. The authors also perform an experiment on the IMP binding element mutant, which is not very conclusive. The second conclusion is novel and shows a potential physiological role of trans regulation. However, there are a few elements worth further clarification, especially with the first conclusion.
1) By deletion of nt121-150, the mutant cannot be rescued (Figure 1). The authors conclude that the 5' UTR is not important for being-rescued in trans. Since the deletion not only disrupts the long form Osk protein, but also the 5' UTR of short form osk RNA, the transcript (D121-150) itself might have problems for translation regulation. In order to make a strong statement that the 5' UTR is not important for rescue of a recipient mRNA, additional mutations or experiments will be needed.
2) It looks like that the RNP assembly is crucial for trans rescue. The mechanism proposed is that the donor and the acceptor mRNA need to be packaged together into an mRNP for this to happen. The authors showed development defects with GFP reporter expression. However, many mutations might result in defects in RNA localization or RNA assembly. Therefore, the distribution of the mutant RNA needs better characterization, for example, by FISH to donor and recipient, not just by qPCR to measure the total amount.
3) The authors argue that PTB dependent RNP assembly is crucial for the trans rescue. It is likely that the RNP assembly is necessary for the rescue. There are many ways that RNP assembly could be disrupted by the mutants. The point is how to prove it is PTB dependent. The authors mutated a set of PTB binding sites and showed rescue defects. There are two points the authors might want to show. First, these mutations may result in no or decreased PTB binding. The second might be hard to prove: so many mutations might disrupt something else. The authors also used a heterozygous mutant PTB to reduce the PTB level (is that true?). But as the author notes, PTB is essential for many RNA functions and might have indirect effects on osk.
4) The inclusion of data from IBE mutations is puzzling and doesn't help the manuscript. It is idiosyncratic, as the authors suggest. I would remove it.
The novelty of the paper lies in showing a mechanism for rescue in trans. The authors provide data that PTB is part of this mechanism but this requires further work. Part of the problem is that they rely almost exclusively on genetics, which leads to uncertainty about epistatic mechanisms leading to their observations. For instance, the increase in Gurken is interesting, but phenomenological. While the proposed mechanism of rescue is likely correct, it needs validation before the manuscript is acceptable. FISH experiments may help to support the proposed mechanism. Two color FISH will help correlate rescue with colocalization. Furthermore, this will show the importance of homotypic clustering in the granules (an approach recently described by the Lehmann lab, 2015, Nature Communications). If the FISH experiments yield the predicted result, they will suggest a mechanism where homotypic interactions are necessary for rescue. As the manuscript now stands, it is not sufficiently novel or definitive for eLife.
In this manuscript, Macdonald et al. have provided strong evidence in support of a model of post-transcriptional regulation in trans. Through their study of oskar and gurken mRNAs, the authors have characterized the regulatory elements required on donor and recipient mRNAs to allow for such regulation to take place. The authors go on to show that mutations in candidate PTB binding sites in the donor mRNA abolish the ability of the donor to regulate the recipient mRNA, and that regulation in trans is dependent on PTB dosage. The authors generalize their model by showing that oskar mRNA is capable of regulating another mRNA with which it co-localizes, gurken, in a manner that once again requires candidate PTB binding sites on the donor. They conclude that PTB-dependent particle assembly mediates regulation in trans.
As noted in the manuscript, previous published experiments from this group had demonstrated the ability to rescue BRE function in the osk transcript in trans (Reveal et al., 2010). These experiments aim to provide additional insights into this phenomenon by studying rescue in trans of osk mutations other than BREs. The authors propose a broader implication for the functional effects of so-called "regulation in trans" beyond the BREs and the osk mRNA, albeit with a limited number of examples. The experiments also provide a convincing mechanism for the long-range regulatory interactions of these mRNAs – through PTB binding pyrimidine tracts (PTs). However, other mechanisms acting on osk, grk, and any other transcripts have not been ruled out.
Furthermore, the authors suggest that regulation in trans may be widespread, such that regulated assembly of different mRNAs into RNPs may make them amenable to regulation by factors that may not bind them directly. This would add a layer of complexity to our current understanding of post-transcriptional regulation, making the regulatory space accessible by such mRNAs much larger, and even more combinatorial in nature than previously thought. However, more evidence would be required to make this generalized conclusion.
Most of the results presented support the conclusions drawn by the authors, but a few supporting experiments are lacking:
Although the data presented in Figures 3 and 4 are consistent with regulation in trans being facilitated by PTB-dependent RNP assembly, the authors have not explicitly demonstrated that this is mediated by direct binding of PTB to oskar mRNA, and that PTB binding is abolished in osk 3'PT mutants. Furthermore, claims that these mutations disrupt particle assembly should be provided in a more direct manner to support the authors' model.
IP of PTB followed by RTqPCR for WT oskar mRNA and the various 3'PT mutants of oskar mRNA would determine whether the candidate binding sites are functional, and whether PTB binding is abolished in the osk3'PT mutants.
If regulation in trans is PTB-dependent, the extent to which PTB binding is disrupted (by IP-RTqPCR or similar approaches) in the various osk3'PT mutants should correlate with their abilities to act as donors.
The role for Bru-mediated osk mRNA oligomerization elements in regulation in trans is proposed but not tested by the authors, citing that the Bru sites must be present to rescue their recipient mRNA (subsection “osk RNP interactions are required for rescue in trans”, second paragraph). However, earlier experiments in this manuscript have shown that rescue in trans is not limited to the BREs, so that other mRNA constructs are available to test this. For example, the rescue of osk3'1004-1008 (Figure 1) by different donors with or without oligomerization elements could be assayed in a similar manner as the osk C BRE- used in Figure 3.
While the mutation of PTs in the osk 3' UTR had a marked effect on Grk protein levels, the authors did not address whether the reciprocal is true. That is, does mutation of the predicted PTs in the grk transcript lead to an increase in wild-type Osk protein levels? If the proposed mechanism of community regulation for transcripts in close proximity is true, effects of regulation in trans should be observed in both directions: osk and grk in close proximity would cross-regulate.https://doi.org/10.7554/eLife.10965.016
- Paul M Macdonald
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
We thank members of the Fischer, Macdonald and Stein labs for comments during the course of the work, Sally Amen of the University of Texas at Austin Department of Statistics and Data Sciences for advice on statistical analysis, Anne Ephrussi for antibodies and the PTB expression plasmid, and Janice Fischer for comments on the manuscript. The 1D12 and 1B1 monoclonal antibodies were obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by The University of Iowa, Department of Biology, Iowa City, Iowa 5224.
- Rachel Green, Reviewing Editor, Johns Hopkins School of Medicine, United States
- Received: August 19, 2015
- Accepted: March 18, 2016
- Version of Record published: April 22, 2016 (version 1)
© 2016, Macdonald et al.
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.