A genome-wide resource for the analysis of protein localisation in Drosophila
- Max Planck Institute of Cell Biology and Genetics, Germany
- Max Planck Institute of Biochemistry, Germany
- Tata Institute of Fundamental Research, India
- Centre for Cellular and Molecular Platforms, National Centre for Biological Sciences, India
- Heidelberg Institute of Theoretical Studies, Germany
- University of California, Los Angeles, United States
- Trinity College Dublin, Ireland
Abstract
The Drosophila genome contains >13,000 protein coding genes, the majority of which remain poorly investigated. Important reasons include the lack of antibodies or reporter constructs to visualise these proteins. Here we present a genome-wide fosmid library of 10,000 GFP-tagged clones, comprising tagged genes and most of their regulatory information. For 880 tagged proteins we created transgenic lines and for a total of 207 lines we assessed protein expression and localisation in ovaries, embryos, pupae or adults by stainings and live imaging approaches. Importantly, we visualised many proteins at endogenous expression levels and found a large fraction of them localising to subcellular compartments. By applying genetic complementation tests we estimate that about two-thirds of the tagged proteins are functional. Moreover, these tagged proteins enable interaction proteomics from developing pupae and adult flies. Taken together, this resource will boost systematic analysis of protein expression and localisation in various cellular and developmental contexts.
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© 2016, Sarov et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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A genome-wide resource for the analysis of protein localisation in Drosophila
eLife 5:e12068.
https://doi.org/10.7554/eLife.12068
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