Structural basis for activation, assembly and membrane binding of ESCRT-III Snf7 filaments
- Cornell University, United States
- The University of Texas Southwestern Medical Center, United States
- The Pennsylvania State University College of Medicine, United States
Abstract
The endosomal sorting complexes required for transport (ESCRTs) constitute hetero-oligomeric machines that catalyze multiple topologically similar membrane-remodeling processes. Although ESCRT-III subunits polymerize into spirals, how individual ESCRT-III subunits are activated and assembled together into a membrane-deforming filament remains unknown. Here, we determine X-ray crystal structures of the most abundant ESCRT-III subunit Snf7 in its active conformation. Using pulsed dipolar electron spin resonance spectroscopy (PDS), we show that Snf7 activation requires a prominent conformational rearrangement to expose protein-membrane and protein-protein interfaces. This promotes the assembly of Snf7 arrays with ~30Å periodicity into a membrane-sculpting filament. Using a combination of biochemical and genetic approaches, both in vitro and in vivo, we demonstrate that mutations on these protein interfaces halt Snf7 assembly and block ESCRT function. The architecture of the activated and membrane-bound Snf7 polymer provides crucial insights into the spatially unique ESCRT-III-mediated membrane remodeling.
Article and author information
Author details
Reviewing Editor
- William I Weis, Stanford University, Bangladesh
Version history
- Received: October 24, 2015
- Accepted: December 13, 2015
- Accepted Manuscript published: December 15, 2015 (version 1)
- Version of Record published: January 13, 2016 (version 2)
Copyright
© 2015, Tang et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Structural basis for activation, assembly and membrane binding of ESCRT-III Snf7 filaments
eLife 4:e12548.
https://doi.org/10.7554/eLife.12548
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