(A) Glycogen metabolism in bacteria. In green: glycogen synthesis. In blue: glycogen degradation. Glc1P is the substrate of GlgC for ADP-Glc synthesis. GlgA (glycogen synthase) produces linear …
Cells were (A) non-infected, or infected with C. trachomatis for (B) 24 hr or (C) 48 hr, fixed in PFA and processed for PAS stain. (D) Enlargement of the boxed region in (B) Note that glycogen …
Cells were infected for 30 hr with C. trachomatis. The picture on the right shows an enlargement of the boxed region. Glycogen is visualized by TEM after PATAg stain. Glycogen deposits in the …
HeLa cells were infected with C. trachomatis for 8, 16, 20, 24 or 48 hr. Glycogen is visualized by TEM after PATAg stain. White arrows point to inclusions. Glycogen is first detected in the …
(A) Gys1 is imported into the inclusion lumen. Cells were treated with siRNA control or against Gys1 prior to infection for 30 hr. DNA was stained in blue, Gys1 in green and the inclusion membrane …
HeLa cells were Glc-deprived (A) or not (B, same medium supplemented with 4.5 mg/ml Glc) for 48 hr, prior to infection with C. trachomatis. Gys1 was stained in green and the inclusion membrane in …
Wild-type (WT) or Atg5-/- mouse embryonic fibroblasts (MEFs) were infected for 30 hr with C. trachomatis. (A) PAS staining. Glycogen accumulation was identical in both cell lines. Scale bar: 10 µm. …
(A) HeLa cells were infected for 24 hr or 48 hr with GFP expressing L2 (GFP-Chlam, green) and stained with an anti-GlgX antibody (red) and Hoechst (blue). Insets to the right show enlargements of …
qRT-PCR of selected genes related to glycogen metabolism was performed. Values were plotted against standard curves and cDNA was normalized with the overall chlamydial genomic DNA (gDNA) quantified …
(A) Western Blot of HeLa cells non-infected (nI) or infected (I) with LGV for 24 hr. The anti-GlgX antibody detected a band of the expected molecular weight (73 kDa), only present in the infected …
HeLa cells were infected for 24 hr with LGV, fixed with 3% PFA and stained with the anti-GlgX antibody (green), an antibody against the inclusion protein CT813 (red) and Hoechst (blue). Insets to …
(A) Purified EBs were incubated for 2 hr with [14C]-Glc, [14C]-Glc6P or [14C]-Glc1P in absence or presence of a 50-fold excess of non-radioactive Glc, Glc6P or Glc1P. Bacteria were subsequently …
(A) PAS staining was performed 24 hr after transfection with chlamydial Flag-GlgA. (B) Zymogram analysis. Serial dilutions of lysates of E. coli lacking endogenous glgA and transformed with …
(A) HeLa cells were transfected (top right) or not (top left) with Flag-GlgA before infection, and fixed 24 hpi. PAS staining revealed an increase of intraluminal glycogen (arrowheads) in Flag-GlgA …
Cells were infected for 40 hr with the plasmid-less strain LGV 25667R. Glycogen is visualized by TEM after PATAg stain and is found in EBs (black arrow heads) and free in the inclusion lumen (white …
(A, B) Prior to infection cells were transfected with SLC35D2-HA, and fixed 24 hpi. (A) Labelling of the HA tag (green), inclusion membrane marker Cap1 (red) and DNA (blue) show recruitment of …
Cells were treated with control siRNA, or siRNA against SLC35D2, or SLC35D2 and Gys1 48 hr and 4 hr prior to infection. (A) Cells were fixed 48 hr after infection and PAS staining was applied. Mean …
Cells were treated with either siRNA control or siRNA against Gys1 48 hr and 4 hr prior to infection with glgA mutants P2B10, P3B4 or with the parental wild-type strain. (A) PAS staining was …
Alignments were obtained using Clustal W. An asterisk indicates positions which have a single, fully conserved residue. A colon points to conservation between groups of strongly similar properties …
Early during the infectious cycle (left) the inclusion contains mostly RBs. This developmental form does not accumulate glycogen and uses ATP rather than Glc6P (Omsland et al., 2012). SLC35D2, and …
Primers used for cloning purposes.
List of siRNAs.
List of primers used in qRT-PCR and RT-PCR.