CELF RNA binding proteins promote axon regeneration in C. elegans and mammals through alternative splicing of Syntaxins
- University of Texas Health Science Center at San Antonio, United States
- University of California, San Diego, United States
- Howard Hughes Medical Institute, University of California, San Diego, United States
Abstract
Axon injury triggers dramatic changes in gene expression. While transcriptional regulation of injury-induced gene expression is widely studied, less is known about the roles of RNA binding proteins (RBPs) in post-transcriptional regulation during axon regeneration. In C. elegans the CELF (CUGBP and Etr-3 Like Factor) family RBP UNC-75 is required for axon regeneration. Using crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq) we identify a set of genes involved in synaptic transmission as mRNA targets of UNC-75. In particular, we show that UNC-75 regulates alternative splicing of two mRNA isoforms of the SNARE Syntaxin/unc-64. In C. elegans mutants lacking unc-75 or its targets, regenerating axons form growth cones, yet are deficient in extension. Extending these findings to mammalian axon regeneration, we show that mouse Celf2 expression is upregulated after peripheral nerve injury and that Celf2 mutant mice are defective in axon regeneration. Further, mRNAs for several Syntaxins show CELF2 dependent regulation. Our data delineate a post-transcriptional regulatory pathway with a conserved role in regenerative axon extension.
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Author details
Reviewing Editor
- Graeme W Davis, University of California, San Francisco, United States
Ethics
Animal experimentation: This study was performed in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. All of the animals were handled according to approved institutional animal care and use committee (IACUC) protocols (S13072) of the University of California. All surgery was performed under anesthesia, and every effort was made to minimize suffering.
Version history
- Received: March 15, 2016
- Accepted: June 1, 2016
- Accepted Manuscript published: June 2, 2016 (version 1)
- Version of Record published: July 15, 2016 (version 2)
Copyright
© 2016, Chen et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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CELF RNA binding proteins promote axon regeneration in C. elegans and mammals through alternative splicing of Syntaxins
eLife 5:e16072.
https://doi.org/10.7554/eLife.16072
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