(a-d) Results of nop-1(RNAi), ani-1(RNAi) and mlc-4(RNAi). Average AP profiles of gel flow (light green, smoothened), compression rates (dark green) and nematic order parameter (blue) at the time of stationary flow during cytokinesis onset. Error bars represent the standard error of the mean (for nop-1, ani-1, mlc-4 5–7 hr and 7–9 hr, N = 9, 18, 9, 13, respectively). Dashed red line indicates the respective best-fit theory predictions for the nematic order parameter (blue). For cytokinesis mlc-4 (7–9 hr) tau is small and is determined to be smaller than 0.5 min. (e-f) Overlay of the different profiles at pseudocleavage and cytokinesis onset, non-RNAi is shown in blue and compression rates in dashed lines. Note that the level of outer ingression was similar to the non-RNAi case for ani-1(RNAi) pseudocleavage, but with improper location and no membrane overlapping, thus no full pseudocleavage furrowing was obtained but a remaining initial anterior ingression was achieved in the presence of some filaments alignment.