A bioengineered niche promotes in vivo engraftment and maturation of pluripotent stem cell derived human lung organoids

  1. Briana R Dye
  2. Priya H Dedhia
  3. Alyssa J Miller
  4. Melinda S Nagy
  5. Eric S White
  6. Lonnie D Shea
  7. Jason R Spence  Is a corresponding author
  1. University of Michigan Medical School, United States

Abstract

Human pluripotent stem cell (hPSC) derived tissues often remain developmentally immature in vitro, and become more adult-like in their structure, cellular diversity and function following transplantation into immunocompromised mice. Previously we have demonstrated that hPSC-derived human lung organoids (HLOs) resembled human fetal lung tissue in vitro (Dye et al. 2015). Here we show that HLOs required a bioartificial microporous Poly(lactide-co-glycolide) (PLG) scaffold niche for successful engraftment, long-term survival, and maturation of lung epithelium in vivo. Analysis of scaffold-grown transplanted tissue showed airway-like tissue with enhanced epithelial structure and organization compared to HLOs grown in vitro. By further comparing in vitro and in vivo grown HLOs with fetal and adult human lung tissue, we found that in vivo transplanted HLOs had improved cellular differentiation of secretory lineages that is reflective of differences between fetal and adult tissue, resulting in airway-like structures that were remarkably similar to the native adult human lung.

Article and author information

Author details

  1. Briana R Dye

    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, United States
    Competing interests
    The authors declare that no competing interests exist.
  2. Priya H Dedhia

    Department of Surgery, University of Michigan Medical School, Ann Arbor, United States
    Competing interests
    The authors declare that no competing interests exist.
  3. Alyssa J Miller

    Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, United States
    Competing interests
    The authors declare that no competing interests exist.
  4. Melinda S Nagy

    Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, United States
    Competing interests
    The authors declare that no competing interests exist.
  5. Eric S White

    Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, United States
    Competing interests
    The authors declare that no competing interests exist.
  6. Lonnie D Shea

    Center for Organogenesis, University of Michigan Medical School, Ann Arbor, United States
    Competing interests
    The authors declare that no competing interests exist.
  7. Jason R Spence

    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, United States
    For correspondence
    spencejr@umich.edu
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-7869-3992

Funding

National Heart, Lung, and Blood Institute (RO1 HL119215)

  • Jason R Spence

Unviersity of Michigan Cellular and Molecular Biology training grant (T32 GM007315)

  • Alyssa J Miller

University of Michigan Tissue Engineering and Regeneration Training Grant (DE00007057)

  • Alyssa J Miller

University of Michigan Rackham Graduate Fellowship

  • Briana R Dye

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Ethics

Animal experimentation: All work using human pluripotent stem cells was approved by the University of Michigan Human Pluiripotent Stem Cell Research Oversight Committee (HPSCRO, application #1054). All human tissue used in this work was falls under NIH Exemption 4. The tissue was not obtained from living individuals, and was de-identified. Since this work falls under NIH Exemption 4, it was given a "not regulated" status by the University of Michigan IRB (protocol # HUM00093465 and HUM00105750). All animal experiments were approved by the University of Michigan Institutional Animal Care and Use Committee (IACUC; protocol # PRO00006609).

Reviewing Editor

  1. Janet Rossant, University of Toronto, Canada

Publication history

  1. Received: July 19, 2016
  2. Accepted: September 21, 2016
  3. Accepted Manuscript published: October 11, 2016 (version 1)
  4. Accepted Manuscript updated: September 29, 2016 (version 2)
  5. Version of Record published: November 1, 2016 (version 3)

Copyright

© 2016, Dye et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

Metrics

  • 6,410
    Page views
  • 1,478
    Downloads
  • 117
    Citations

Article citation count generated by polling the highest count across the following sources: Scopus, Crossref, PubMed Central.

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Open citations (links to open the citations from this article in various online reference manager services)

Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)

  1. Briana R Dye
  2. Priya H Dedhia
  3. Alyssa J Miller
  4. Melinda S Nagy
  5. Eric S White
  6. Lonnie D Shea
  7. Jason R Spence
(2016)
A bioengineered niche promotes in vivo engraftment and maturation of pluripotent stem cell derived human lung organoids
eLife 5:e19732.
https://doi.org/10.7554/eLife.19732

Further reading

    1. Stem Cells and Regenerative Medicine
    Marko Z Nikolić, Emma L Rawlins
    Insight

    Transplanting bioengineered human lung organoids into mice could lead to a humanized model for pre-clinical studies of lung disease.

    1. Stem Cells and Regenerative Medicine
    Rute A Tomaz et al.
    Tools and Resources

    Production of large quantities of hepatocytes remains a major challenge for a number of clinical applications in the biomedical field. Directed differentiation of human pluripotent stem cells (hPSCs) into hepatocyte-like cells (HLCs) provides an advantageous solution and a number of protocols have been developed for this purpose. However, these methods usually follow different steps of liver development in vitro, which is time consuming and requires complex culture conditions. In addition, HLCs lack the full repertoire of functionalities characterising primary hepatocytes. Here, we explore the interest of forward programming to generate hepatocytes from hPSCs and to bypass these limitations. This approach relies on the overexpression of three hepatocyte nuclear factors (HNF1A, HNF6, and FOXA3) in combination with different nuclear receptors expressed in the adult liver using the OPTi-OX platform. Forward programming allows for the rapid production of hepatocytes (FoP-Heps) with functional characteristics using a simplified process. We also uncovered that the overexpression of nuclear receptors such as RORc can enhance specific functionalities of FoP-Heps thereby validating its role in lipid/glucose metabolism. Together, our results show that forward programming could offer a versatile alternative to direct differentiation for generating hepatocytes in vitro.