(a) Embryonic elongation in C. elegans is driven in part by epidermal actomyosin contractility and in part by muscle contractions. The length of the embryo is used for staging: 2-fold (2F) stage means roughly 2-fold increase in length from the beginning of elongation. Representative stages are shown; anterior to the left, dorsal up. (b, c, d) Actin filament organization at the 1.3F, 1.5F and 1.7F stages, respectively, visualized with an ABD::GFP marker. Actin filaments progressively organize into circumferential parallel bundles in DV cells (arrows), arrowheads point to seam cells. Note that the integrated ABD::GFP marker shows some cell to cell variation in expression. (b’, b”, c’, c”, d’, d”) Close-up images of actin pattern in DV cells (from the area in the white rectangle) and seam cells (from the area in the pink rectangle), respectively, of the images in (b), (c) and (d) respectively. (e) Actomyosin forces squeeze the embryo circumferentially to make it elongate in the antero-posterior direction. (f, g) Endogenous distribution of the two non-muscle myosin II isoforms visualized with the CRISPR GFP-labelled myosin heavy chains NMY-2 (f) and NMY-1 (g). Arrowheads point to seam cells, which are delineated by the junctional marker DLG-1::RFP.