(A) Quantitative co-localisation analysis for FLS2-GFP or BRI1-GFP, respectively, with FLS2-mCherry after transient co-expression in epidermal leaf cells of N. benthamiana. (B) Quantitative co-localisation analysis for FLS2-GFP or BRI1-GFP, respectively, with FLS2-mCherry after transient co-expression in epidermal leaf cells of N. benthamiana and BRZ-treatment. The coloured data points indicate the values of technical replicates; black dots indicate the position of outliers. To assess whether the determined co-localisation values (original or ori.) were significant, co-localisation analysis was also carried out after image randomisation. For this purpose, one of the two image channels was rotated by 90 degrees prior to co-localisation analysis (rotated or rot.). In addition, the co-localisation values of a reciprocal experiment are shown. Here, co-localisation of FLS2-GFP or BRI1-GFP was determined with regard to BRI1-mRFP. As emphasised by the represented p-values, we observed a specific co-localisation for FLS2-GFP and FLS2-mCherry. In contrast, the co-localisation of BRI1-GFP with FLS2-mCherry was not different from randomised images, and thus non-specific. Similar observations were made after BRZ-treatment for 2 days to deplete endogenous BRs as well as for reciprocal experiments using BRI1-mRFP as reference. The indicated p-values were obtained using a two-tailed heteroscedastic t-test and a Bonferroni multiple hypothesis correction.