Plant immune and growth receptors share common signalling components but localise to distinct plasma membrane nanodomains
- The Sainsbury Laboratory, United Kingdom
- University of Cambridge, United Kingdom
- Earlham Institute, United Kingdom
- Seoul National University, Republic of Korea
- Université de Toulouse, CNRS, UPS, France
- University of Freiburg, Germany
Abstract
Cell surface receptors govern a multitude of signalling pathways in multicellular organisms. In plants, prominent examples are the receptor kinases FLS2 and BRI1, which activate immunity and steroid-mediated growth, respectively. Intriguingly, despite inducing distinct signalling outputs, both receptors employ common downstream signalling components, which exist in plasma membrane (PM)-localised protein complexes. An important question is thus how these receptor complexes maintain signalling specificity. Live-cell imaging revealed that FLS2 and BRI1 form PM nanoclusters. Using single-particle tracking we could discriminate both cluster populations and we observed spatiotemporal separation between immune and growth signalling platforms. This finding was confirmed by visualising FLS2 and BRI1 within distinct PM nanodomains marked by specific remorin proteins and differential co-localisation with the cytoskeleton. Our results thus suggest that signalling specificity between these pathways may be explained by the spatial separation of FLS2 and BRI1 with their associated signalling components within dedicated PM nanodomains.
Article and author information
Author details
Christian Schudoma
Funding
Gatsby Charitable Foundation
- Silke Robatzek
- Daniel MacLean
- Cyril Zipfel
H2020 European Research Council
- Cyril Zipfel
Deutsche Forschungsgemeinschaft
- Thomas Ott
Universitat Bayern
- Iris Katharina Jarsch
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2017, Bücherl et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
Metrics
-
- 7,871
- views
-
- 2,231
- downloads
-
- 205
- citations
Views, downloads and citations are aggregated across all versions of this paper published by eLife.
Citations by DOI
-
- 205
- citations for umbrella DOI https://doi.org/10.7554/eLife.25114
Download links
A two-part list of links to download the article, or parts of the article, in various formats.
Downloads (link to download the article as PDF)
Open citations (links to open the citations from this article in various online reference manager services)
Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)
Plant immune and growth receptors share common signalling components but localise to distinct plasma membrane nanodomains
eLife 6:e25114.
https://doi.org/10.7554/eLife.25114
