1. Cell Biology
  2. Plant Biology
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Plant immune and growth receptors share common signalling components but localise to distinct plasma membrane nanodomains

  1. Christoph A Bücherl
  2. Iris Katharina Jarsch
  3. Christian Schudoma
  4. Cécile Segonzac
  5. Malick Mbengue
  6. Silke Robatzek
  7. Daniel MacLean
  8. Thomas Ott
  9. Cyril Zipfel  Is a corresponding author
  1. The Sainsbury Laboratory, United Kingdom
  2. University of Cambridge, United Kingdom
  3. Earlham Institute, United Kingdom
  4. Seoul National University, Republic of Korea
  5. Université de Toulouse, CNRS, UPS, France
  6. University of Freiburg, Germany
Research Article
  • Cited 90
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Cite this article as: eLife 2017;6:e25114 doi: 10.7554/eLife.25114
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Abstract

Cell surface receptors govern a multitude of signalling pathways in multicellular organisms. In plants, prominent examples are the receptor kinases FLS2 and BRI1, which activate immunity and steroid-mediated growth, respectively. Intriguingly, despite inducing distinct signalling outputs, both receptors employ common downstream signalling components, which exist in plasma membrane (PM)-localised protein complexes. An important question is thus how these receptor complexes maintain signalling specificity. Live-cell imaging revealed that FLS2 and BRI1 form PM nanoclusters. Using single-particle tracking we could discriminate both cluster populations and we observed spatiotemporal separation between immune and growth signalling platforms. This finding was confirmed by visualising FLS2 and BRI1 within distinct PM nanodomains marked by specific remorin proteins and differential co-localisation with the cytoskeleton. Our results thus suggest that signalling specificity between these pathways may be explained by the spatial separation of FLS2 and BRI1 with their associated signalling components within dedicated PM nanodomains.

Article and author information

Author details

  1. Christoph A Bücherl

    The Sainsbury Laboratory, Norwich, United Kingdom
    Competing interests
    The authors declare that no competing interests exist.
  2. Iris Katharina Jarsch

    Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, United Kingdom
    Competing interests
    The authors declare that no competing interests exist.
  3. Christian Schudoma

    Earlham Institute, Norwich, United Kingdom
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-1157-1354
  4. Cécile Segonzac

    Department of Plant Science, Plant Genome and Breeding Institute, Seoul National University, Seoul, Republic of Korea
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-5537-7556
  5. Malick Mbengue

    Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, Castanet-Tolosan, France
    Competing interests
    The authors declare that no competing interests exist.
  6. Silke Robatzek

    The Sainsbury Laboratory, Norwich, United Kingdom
    Competing interests
    The authors declare that no competing interests exist.
  7. Daniel MacLean

    The Sainsbury Laboratory, Norwich, United Kingdom
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-1032-0887
  8. Thomas Ott

    Faculty of Biology, Cell Biology, University of Freiburg, Freiburg, Germany
    Competing interests
    The authors declare that no competing interests exist.
  9. Cyril Zipfel

    The Sainsbury Laboratory, Norwich, United Kingdom
    For correspondence
    cyril.zipfel@tsl.ac.uk
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-4935-8583

Funding

Gatsby Charitable Foundation

  • Silke Robatzek
  • Daniel MacLean
  • Cyril Zipfel

H2020 European Research Council

  • Cyril Zipfel

Deutsche Forschungsgemeinschaft

  • Thomas Ott

Universitat Bayern

  • Iris Katharina Jarsch

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Thorsten Nürnberger, University of Tubingen, Germany

Publication history

  1. Received: January 13, 2017
  2. Accepted: March 4, 2017
  3. Accepted Manuscript published: March 6, 2017 (version 1)
  4. Version of Record published: April 6, 2017 (version 2)

Copyright

© 2017, Bücherl et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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