Chloroidium strains were isolated from samples taken from the indicated locations in (a) the UAE, and (b) within Abu Dhabi city specifically. Chloroidium strains were found in estuaries, mangrove …
(a) Cell size distribution of Chloroidium sp. UTEX 3007 in late log phase (14 days). Cell size analysis was performed using a Cellometer Auto M10 from Nexcelcom Bioscience (Lawrence, MA, USA) on 2 × …
Cell diameter measurements.
Cell concentration time course measurements.
Flow cytometry measurements.
(a) Chloroidium sp. UTEX 3007 fatty acid content estimated by extracting total lipid, creating methyl esters, and running the esters on a GC-FID (whiskers = range, box boundaries = 1 SD, center box …
GC-FID results for major fatty acid species in Chloroidium sp. UTEX 3007.
Fatty acid profiles of Chloroidium sp. UTEX 3007, Elaeis guineensis (Barcelos et al., 2015), and several other algal isolates (Lang et al., 2011).
HPLC-MS base peak chromatograms (BPCs) for Chloroidium sp. UTEX 3007 and Chlamydomonas reinhardtii extracts.
(a) Biolog phenotype microarrays were run using an Omnilog instrument (Biolog Inc., Hayward, USA) as previously described (Chaiboonchoe et al., 2014). In total, 380 substrate utilization assays for …
Phenotype microarray results for plates PM1, PM2, and PM3.
GC-MS results for Chloroidium sp. UTEX 3007 (Cm) and Chlamydomonas reinhardtii (Cre) intracellular polar metabolites.
Functional protein family domains in a Chloroidium sp. UTEX 3007 predicted protein with high similarity to PLD proteins from closely related organisms. PLDs are members of the phospholipase …
Locations, confidence scores, and accession numbers for PLD and C2 Pfam domains in Chloroidium sp. UTEX 3007.
Well-curated assemblies from genomes of algae from four other clades were downloaded from NCBI [assemblies - Micromonas pusilla, Ostreococcus taurii, and Coccomyxa subellipsoidea]. The Chlamydomonas …
Predicted Pfam designations for each species in Figure 6.
Table with QUAST results used in (b).
Interactive Venn diagram that can be viewed at interactivenn.net to obtain Pfam sets for numbers displayed in Figure 6.
(a) Gene models for the putative manganese catalases (CDS1/2/3). Functional annotation of homologs from (CDS1/2/3) is limited: 96 of the top 100 BLAST hits of CDS3 in the non-redundant database (nr …
Models of CDS1-3 in protein data bank (PDB) format.
Amino acid residue alignment of CDS1-3.
Metal-binding motif residues are highlighted.
Protein families (Pfams) with roles in (a) abiotic stress resistance and (b) saccharide metabolism unique to Chloroidium among the green algae explored. Pfam domains were predicted using HMMsearch …
Description | Key | i-Evalue | |
---|---|---|---|
(a) | Iron-containing redox enzyme | Haem_oxygenas_2 | 7.50E-05 |
Salt tolerance down-regulator | NST1 | 0.00012 | |
Proline-rich | Pro-rich | 0.00015 | |
Peroxisome biogenesis factor | PEX-2N | 0.003 | |
OsmC-like protein | OsmC | 0.0066 | |
NA,K-Atpase interacting protein | NKAIN | 0.003 | |
Metallothionein | Metallothio_2 | 0.0074 | |
Mercuric transport protein | MerT | 0.0054 | |
(b) | Beta-galactosidase jelly roll domain | BetaGal_dom4_5 | 0.013 |
Cryptococcal mannosyltransferase | CAP59_mtransfer | 3.50E-15 | |
Carbohydrate binding domain CBM49 | CBM49 | 0.0085 | |
Carbohydrate binding domain (family 25) | CBM_25 | 0.0023 | |
Carbohydrate binding domain | CBM_4_9 | 0.00036 | |
Cellulose biosynthesis protein BcsN | CBP_BcsN | 0.0018 | |
Glycosyl hydrolase family 70 | Glyco_hydro_70 | 0.00054 | |
Glycosyl hydrolase family 9 (heptosyltransferase) | Glyco_transf_9 | 0.0025 | |
Carbohydrate esterase/acetylesterase | SASA | 2.00E-06 | |
Activator of aromatic catabolism | XylR_N | 0.011 |