(A) Phylogenetic tree of vertebrate murf1, 2, 3 and 4 (also known as trim63, 55, 54 and 101, respectively). The tree was constructed using ClustalX with the neighbor-joining method. Zebrafish (z), Human (h), mouse (m), rat (r), chick (g), frog (x). (B) Alignment of murf1 genes from human, mouse and zebrafish. Blue boxes highlight identical amino acids and grey boxes indicate similar residues. (C) Whole-mount in situ hybridization demonstrating the expression patterns of murf1a and murf1b in the zebrafish embryo.