Dynamics of the IFT machinery at the ciliary tip
- University of California, Berkeley, United States
- University of Minnesota, United States
Abstract
Intraflagellar transport (IFT) is essential for the elongation and maintenance of eukaryotic cilia and flagella. Due to the traffic jam of multiple trains at the ciliary tip, how IFT trains are remodeled in these turnaround zones cannot be determined by conventional imaging. Using Photogate, we visualized the full range of movement of single IFT trains and motors in Chlamydomonas flagella. Anterograde trains split apart and IFT complexes mix with each other at the tip to assemble retrograde trains. Dynein-1b is carried to the tip by kinesin-II as inactive cargo on anterograde trains. Unlike dynein-1b, kinesin-II detaches from IFT trains at the tip and diffuses in flagella. As the flagellum grows longer, diffusion delays return of kinesin-II to the basal body, depleting kinesin-II available for anterograde transport. Our results suggest that dissociation of kinesin-II from IFT trains serves as a negative feedback mechanism that facilitates flagellar length control in Chlamydomonas.
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Funding
National Institute of General Medical Sciences (GM094522)
- Ahmet Yildiz
National Science Foundation (MCB-1055017)
- Ahmet Yildiz
National Institute of General Medical Sciences (GM055667)
- Mary E Porter
National Science Foundation (MCB-1617028)
- Ahmet Yildiz
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2017, Chien et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Dynamics of the IFT machinery at the ciliary tip
eLife 6:e28606.
https://doi.org/10.7554/eLife.28606
