Natural variation in stochastic photoreceptor specification and color preference in Drosophila
- Johns Hopkins University, United States
- New York University, United States
- University of Virginia, United States
- Paris-Saclay Institute of Neuroscience, Université Paris Sud, Centre National de la Recherche Scientifque, Université Paris-Saclay, France
Abstract
Each individual perceives the world in a unique way, but little is known about the genetic basis of variation in sensory perception. In the fly eye, the random mosaic of color-detecting R7 photoreceptor subtypes is determined by stochastic ON/OFF expression of the transcription factor Spineless (Ss). In a genome-wide association study, we identified a naturally occurring insertion in a regulatory DNA element in ss that lowers the ratio of SsON to SsOFF cells. This change in photoreceptor fates shifts the innate color preference of flies from green to blue. The genetic variant increases the binding affinity for Klumpfuss (Klu), a zinc finger transcriptional repressor that regulates ss expression. Klu is expressed at intermediate levels to determine the normal ratio of SsON to SsOFF cells. Thus, binding site affinity and transcription factor levels are finely tuned to regulate stochastic expression, setting the ratio of alternative fates and ultimately determining color preference.
Article and author information
Author details
Funding
National Eye Institute (R01EY025598)
- Robert J Johnston
Pew Charitable Trusts (27373)
- Robert J Johnston
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Simon G Sprecher, University of Fribourg, Switzerland
Version history
- Received: June 14, 2017
- Accepted: December 15, 2017
- Accepted Manuscript published: December 18, 2017 (version 1)
- Accepted Manuscript updated: December 22, 2017 (version 2)
- Accepted Manuscript updated: December 23, 2017 (version 3)
- Version of Record published: December 27, 2017 (version 4)
- Version of Record updated: January 12, 2018 (version 5)
- Version of Record updated: April 16, 2018 (version 6)
Copyright
© 2017, Anderson et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Natural variation in stochastic photoreceptor specification and color preference in Drosophila
eLife 6:e29593.
https://doi.org/10.7554/eLife.29593
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