LINE-1 protein localization and functional dynamics during the cell cycle
- NYU Langone Medical Center, United States
- The Rockefeller University, United States
Abstract
LINE-1/L1 retrotransposon sequences comprise 17% of the human genome. Among the many classes of mobile genetic elements, L1 is the only autonomous retrotransposon that still drives human genomic plasticity today. Through its co-evolution with the human genome, L1 has intertwined itself with host cell biology. However, a clear understanding of L1's lifecycle and the processes involved in restricting its insertion and intragenomic spread remains elusive. Here we identify modes of L1 proteins' entrance into the nucleus, a necessary step for L1 proliferation. Using functional, biochemical, and imaging approaches, we also show a clear cell cycle bias for L1 retrotransposition that peaks during the S phase. Our observations provide a basis for novel interpretations about the nature of nuclear and cytoplasmic L1 ribonucleoproteins (RNPs) and the potential role of DNA replication in L1 retrotransposition.
Data availability
Article and author information
Author details
Funding
National Institutes of Health (P50GM107632)
- Jef D Boeke
National Cancer Institute (NIH/NCI P30CA16087)
- Chi Y Yun
National Institutes of Health (1S10OD010582)
- Chi Y Yun
NYSTEM (Contract C026719)
- Chi Y Yun
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2018, Mita et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
Download links
A two-part list of links to download the article, or parts of the article, in various formats.
Downloads (link to download the article as PDF)
Open citations (links to open the citations from this article in various online reference manager services)
Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)
LINE-1 protein localization and functional dynamics during the cell cycle
eLife 7:e30058.
https://doi.org/10.7554/eLife.30058
