The yeast Target of Rapamycin Complex 1 (TORC1) plays a central role in controlling growth. How amino acids and other nutrients stimulate its activity via the Rag/Gtr GTPases remains poorly understood. We here report that the signal triggering Rag/Gtr-dependent TORC1 activation upon amino-acid uptake is the coupled H+ influx catalyzed by amino-acid/H+ symporters. H+-dependent uptake of other nutrients, ionophore-mediated H+ diffusion, and inhibition of the vacuolar V-ATPase also activate TORC1. As the increase in cytosolic H+ elicited by these processes stimulates the compensating H+-export activity of the plasma membrane H+-ATPase (Pma1), we have examined whether this major ATP-consuming enzyme might be involved in TORC1 control. We find that when the endogenous Pma1 is replaced with a plant H+-ATPase, H+ influx or increase fails to activate TORC1. Our results show that H+ influx coupled to nutrient uptake stimulates TORC1 activity and that Pma1 is a key actor in this mechanism.
- Bruno Andre
- Elie Saliba
- Christos Gournas
- Minoas Evangelinos
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
- Michael N Hall, Reviewing Editor, University of Basel, Switzerland
- Received: September 13, 2017
- Accepted: March 22, 2018
- Accepted Manuscript published: March 23, 2018 (version 1)
© 2018, Saliba et al.
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