(A) Western blot analysis of expression of Nups (color-coded by subcomplex as indicated in panel B), selected NTRs, and CPSF6 in T cell (MT4, CEM X174, Jurkat, SupT1, H9), adherent cell (HeLa, HOS, HT1080, 293T), or monocytic cell (THP-1, K-562, U937) lines. Where with multiple bands were detected, arrowheads indicate the band whose migration most closely matched the predicted molecular weight of the Nup/NTR. The indicated bands were utilized for quantification in Figure 2—figure supplement 1, and correspond to the band highlighted in Figure 5. Each well was loaded with 10 μL of lysate containing 104 cells. Each blot represents one of at least three replicates produced from two or three separately generated cell lysates. (B) Schematic representation of the nuclear pore complex listing individual members of each subcomplex, color-coded to correspond to the labels for the western blot in panel (A).