Timing of ESCRT-III protein recruitment and membrane scission during HIV-1 assembly
- The Rockefeller University, United States
- Hofstra University, United States
Figures
Figure 1 with 8 supplements
ESCRT-IIIs and VPS4A transiently recruited prior to scission.
(A) Example trace of Gag-pHluorin assembling into single VLP while the pCO2 in the imaging media was repeatedly switched between 0% and 10% every 10 s. Moment of scission is indicated by red dashed …
Figure 1—figure supplement 1
Flow chamber configuration for ESCRT-III assisted membrane scission studies.
Imaging media in reservoirs was preequilibrated with gas containing 0 and 10% CO2 (balanced with air). During assembly of single HIV particles in cells, the imaging media was modulated between …
Figure 1—figure supplement 2
Example traces of scission relative to recruitment of ESCRT-III or VPS4A.
(A) Gag-pHluorin was observed as pCO2 was cycled between 0 and 10% every 10 s. VLP scission time (red dashed line) was characterized by half drop in lock-in signal. mCherry-CHMP4B, mCherry-CHMP2A, …
Figure 1—figure supplement 3
Knockdown of CHMP2A or CHMP2B by siRNA.
HeLa cell lines stably expressing either mEGFP-CHMP2A or mEGFP-CHMP2B were transfected with siRNA to either CHMP2A or CHMP2B or a control siRNA. 48 hr after transfection, presence of tagged ESCRT …
Figure 1—figure supplement 4
CHMP4B was recruited prior to VPS4A.
The times associated with the rising fluorescence edge of VPS4A and CHMP4B were compared relative to each other. CHMP4B appeared on average 5.1 s prior to VPS4A.
Figure 1—video 1
Gag-pHluorin (left side of video) and mCherry-CHMP4B (right side) were imaged while the CO2 in the media was modulated between 0 and 10% every 10 s.
https://doi.org/10.7554/eLife.36221.008
Figure 1—video 2
Example of individual puncta of Gag-pHluorin assembly (left side, CO2 switching every 10 s) with mCherry-CHMP4B (right side) recruitment.
CHMP4B begins to appear at 13:29 min.
Figure 1—video 3
Gag-pHluorin (top of video) and mCherry-VPS4A (bottom) were imaged while the CO2 in the media was modulated between 0 and 10% every 10 s.
https://doi.org/10.7554/eLife.36221.010
Figure 1—video 4
Example of individual puncta of Gag-pHluorin assembly (left side, CO2 switching every 10 s) with mCherry-VPS4A (right side) recruitment.
VPS4A begins to appear at 20:49 min.
Figure 2 with 2 supplements
Scission more likely at lower cytoplasmic pH.
(A) Example traces of Gag-pHluorin assembly while pCO2 was switched every 120 s between 0% (red dashed line, greater fluorescence emission) and 10% (black dashed line, lower fluorescence emission). …
Figure 2—video 1
Gag-pHluorin (left side of video) and mCherry-VPS4A (right side) were imaged while the CO2 in the media was modulated between 0 and 10% every 120 s.
https://doi.org/10.7554/eLife.36221.013
Figure 2—video 2
Example of individual puncta of Gag-pHluorin assembly (left side, CO2 switching every 120 s) with mCherry-VPS4A (right side) recruitment.
VPS4A begins to appear at 14:33 min.
Figure 3
A new round of ESCRT-III recruitment required following failed scission event.
(A) Example traces of multiple waves of VPS4A and CHMP4B recruited following cessation of Gag accumulation. (B) Example trace of multiple recruitments of VPS4A prior to scission (red dashed line). (C…
Figure 4 with 4 supplements
Structural changes in VLPs throughout Gag accumulation.
(A) Example traces of wild-type Gag-GFP (black line, sf3 in Matrix of Gag) and Gag-∆p6 (grey line, missing ESCRT-I recruiting p6 domain) assembling into single VLPs. Images were collected every 30 s …
Figure 4—figure supplement 1
Example traces of Gag accumulation (quantified as P+2S) and fluorophore polarization (quantified as P/S) during VLP assembly.
Three different tagging schemes were used: labeled with mEGFP after p6 domain at carboxy terminal of Gag (A), labeled with circularly permutated GFP variant three in matrix of Gag (B), labeled with …
Figure 4—figure supplement 2
Example traces of Gag-∆p6-mEGFP accumulation (quantified as P+2S) and fluorophore polarization (quantified as P/S) during VLP assembly.
https://doi.org/10.7554/eLife.36221.018
Figure 4—video 1
Images of Gag-mEGFP were acquired with excitation perpendicular (P) or parallel (S) to the glass surface.
Left side of video is calculated P+2S (quantification of Gag intensity) and right side is ratio image P/S (measurement of structure).
Figure 4—video 2
Example of individual puncta of Gag-mEGFP with P/S and P+2S images.
https://doi.org/10.7554/eLife.36221.020
Figure 5
Proposed temporal model of ESCRT-III-mediated scission of HIV from cell plasma membrane.
(I) The viral particle structure changes throughout accumulation of Gag until (II) a spherical topology prevents incorporation of additional Gags. (III) ESCRT-IIIs (examples: CHMP2, CHMP4) are …
Author response image 1
Measurement of the lag in the rise to ½ max between CHMPB and VPS4A in HEK293T cells.
https://doi.org/10.7554/eLife.36221.023Tables
Key resources table
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (H. sapiens) | HEK-293T | Bieniasz Lab | ||
| Cell line (H. sapiens) | HeLa | ATCC | ||
| Transfected construct (HIV-1, A. victoria) | Gag-mEGFP | Bleck et al. (2014) | ||
| Transfected construct (HIV, A. macrodactyla) | Gag-mTagBFP | Bleck et al. (2014) | ||
| Transfected construct (A. victoria, H. sapiens) | pLNCX2-mEGFP-VPS4A | Bleck et al. (2014) | ||
| Transfected construct (A. marginale, H. sapiens) | pLNCX2-mCherry-CHMP4B | Bleck et al. (2014) | ||
| Transfected construct (HIV) | Gag∆p6 | Bleck et al. (2014) | ||
| Transfected construct (HIV) | pCR3.1/Syn-Gag | Jouvenet et al. (2008) | ||
| Transfected construct (HIV, A. victoria) | pCR3.1/Syn-Gag-pHluorin | Jouvenet et al. (2008) | ||
| Transfected construct (A. marginale, H. sapiens) | pLNCX2-mCherry-CHMP2A | This study | CHMP4B in pLNCX2-mCherry- CHMP4B was replaced by CHMP2A from HEK293T cDNA library | |
| Transfected construct (A. marginale, H. sapiens) | pLNCX2-mCherry-CHMP2B | This study | CHMP4B in pLNCX2-mCherry- CHMP4B was replaced by CHMP2B from HEK293T cDNA library | |
| Transfected construct (A. marginale, H. sapiens) | pLNCX2-mCherry-CHMP2A- siRNAres | This study | added six silent point mutations to pLNCX2-mCherry-CHMP2A using site directed mutagenesis | |
| Transfected construct (A. marginale, H. sapiens) | pLNCX2-mCherry-CHMP2B- siRNAres | This study | added six silent point mutations to pLNCX2-mCherry-CHMP2B using site directed mutagenesis | |
| Transfected construct (A. marginale, H. sapiens) | pLNCX2-mEGFP-CHMP2A | This study | replaced mCherry in pLNCX2- mCherry-CHMP2A with EGFP (pEGFP-N1, Clontech) | |
| Transfected construct (A. marginale, H. sapiens) | pLNCX2-mEGFP-CHMP2B | This study | replaced mCherry in pLNCX2- mCherry-CHMP2B with EGFP (pEGFP-N1, Clontech) | |
| Transfected construct (A. marginale, H. sapiens) | pLNCX2-mCherry-VPS4A | This study | replaced mEGFP in pLNCX2- mCherry-VPS4A (Bleck et al., 2014) with mCherry (pmCherry- N1, Clontech) | |
| Transfected construct (HIV, A. victoria) | Gag-∆p6-mEGFP | This study | p6 domain removed from Gag-mEGFP using site directed mutagensis | |
| Transfected construct (HIV, A. victoria) | Gag-MA-sf3 | This study | sf3 GFP (Pédelacq et al., 2006) inserted in pCR3.1/Syn-Gag | |
| Transfected construct (HIV, A. victoria) | Gag-MA-sf11 | This study | sf11 GFP (Pédelacq et al., 2006) inserted in pCR3.1/Syn-Gag | |
| Sequence-based reagent | CHMP2A DsiRNA | Integrated DNA Technologies | 5'-AAGAUGAAGAGGAG AGUGAUGCUdGdT-3' | |
| Sequence-based reagent | CHMP2A DsiRNA (reverse) | Integrated DNA Technologies | 5'-ACAGCAUCACUCUC CUCUUCAUCUUCC-3' | |
| Sequence-based reagent | CHMP2B DsiRNA | Integrated DNA Technologies | 5'-GGAACAGAAUCGAG AGUUACGAGdGdT-3' | |
| Sequence-based reagent | CHMP2B DsiRNA (reverse) | Integrated DNA Technologies | 5'-ACCUCGUAACUCUC GAUUCUGUUCCUU-3' | |
| Chemical compound, drug | DMEM | Thermo Fisher Scientific | #11965 | |
| Chemical compound, drug | FBS | MilliporeSigma | #F4135 | |
| Chemical compound, drug | HEPES | MilliporeSigma | #H3375 | |
| Software, algorithm | Metamorph | Molecular Devices | version 7.8.10 | |
| Software, algorithm | ImageJ | Schneider et al. (2012) | version Fiji | |
| Software, algorithm | LabView | National Instruments | version 2013 | |
| Software, algorithm | Microscope-Control | Johnson, 2018c) | LabView Code | |
| Software, algorithm | Average-puncta-center | Johnson, 2018a) | LabView Code | |
| Software, algorithm | Puncta-Fit | Johnson, 2018d) | LabView Code | |
| Software, algorithm | CO2-switch-analysis | Johnson, 2018b) | LabView Code |
Additional files
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Transparent reporting form
- https://doi.org/10.7554/eLife.36221.022
