Timing of ESCRT-III protein recruitment and membrane scission during HIV-1 assembly

  1. Daniel S Johnson
  2. Marina Bleck
  3. Sanford M Simon  Is a corresponding author
  1. The Rockefeller University, United States

Abstract

The Endosomal Sorting Complexes Required for Transport III (ESCRT-III) proteins are critical for cellular membrane scission processes with topologies inverted relative to clathrin-mediated endocytosis. Some viruses appropriate ESCRT-IIIs for their release. By imaging single assembling viral-like particles of HIV-1, we observed that ESCRT-IIIs and the ATPase VPS4 arrive after most of the virion membrane is bent, linger for tens of seconds, and depart ~20 seconds before scission. These observations suggest ESCRT-IIIs are recruited by a combination of membrane curvature and the late domains of the HIV-1 Gag protein. ESCRT-IIIs may pull the neck into a narrower form but must leave to allow scission. If scission does not occur within minutes of ESCRT departure, ESCRT-III and VPS4 are recruited again. This mechanistic insight is likely relevant for other ESCRT dependent scission processes including cell division, endosome tubulation, multivesicular body and nuclear envelope formation, and secretion of exosomes and ectosomes.

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Data generated or analyzed during this study are included in the manuscript and supporting files.

Article and author information

Author details

  1. Daniel S Johnson

    Laboratory of Cellular Biophysics, The Rockefeller University, New York, United States
    Competing interests
    The authors declare that no competing interests exist.
  2. Marina Bleck

    Laboratory of Cellular Biophysics, The Rockefeller University, New York, United States
    Competing interests
    The authors declare that no competing interests exist.
  3. Sanford M Simon

    Laboratory of Cellular Biophysics, The Rockefeller University, New York, United States
    For correspondence
    Sanford.Simon@rockefeller.edu
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-8615-4224

Funding

National Institutes of Health (5R01GM119585)

  • Daniel S Johnson
  • Marina Bleck
  • Sanford M Simon

National Institutes of Health (2U54GM103297)

  • Daniel S Johnson
  • Marina Bleck
  • Sanford M Simon

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Copyright

© 2018, Johnson et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Daniel S Johnson
  2. Marina Bleck
  3. Sanford M Simon
(2018)
Timing of ESCRT-III protein recruitment and membrane scission during HIV-1 assembly
eLife 7:e36221.
https://doi.org/10.7554/eLife.36221

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https://doi.org/10.7554/eLife.36221