Thioredoxin shapes the C. elegans sensory response to Pseudomonas produced nitric oxide
- Massachusetts General Hospital, United States
- Harvard University, United States
Abstract
Nitric oxide (NO) is released into the air by NO-producing organisms; however, it is unclear if animals utilize NO as a sensory cue. We show that C. elegans avoids Pseudomonas aeruginosa (PA14) in part by detecting PA14-produced NO. PA14 mutants deficient for NO production fail to elicit avoidance and NO donors repel worms. PA14 and NO avoidance are mediated by a chemosensory neuron (ASJ) and these responses require receptor guanylate cyclases and cyclic nucleotide gated ion channels. ASJ exhibits calcium increases at both the onset and removal of NO. These NO-evoked ON and OFF calcium transients are affected by a redox sensing protein, TRX-1/thioredoxin. TRX-1's trans-nitrosylation activity inhibits the ON transient whereas TRX-1's de-nitrosylation activity promotes the OFF transient. Thus, C. elegans exploits bacterially produced NO as a cue to mediate avoidance and TRX-1 endows ASJ with a bi-phasic response to NO exposure.
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All data generated or analysed during this study are included in the manuscript and supporting files.
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Funding
National Institutes of Health (DK80215)
- Joshua M Kaplan
National Institutes of Health (DC009852)
- Yun Zhang
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2018, Hao et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Thioredoxin shapes the C. elegans sensory response to Pseudomonas produced nitric oxide
eLife 7:e36833.
https://doi.org/10.7554/eLife.36833
