1. Immunology and Inflammation

TAPBPR mediates peptide dissociation from MHC class I using a leucine lever

  1. F Tudor Ilca
  2. Andreas Neerincx
  3. Clemens Hermann
  4. Ana Marcu
  5. Stefan Stevanović
  6. Janet E Deane
  7. Louise H Boyle  Is a corresponding author
  1. University of Cambridge, United Kingdom
  2. University of Cape Town, South Africa
  3. University of Tübingen, Germany
  4. DKFZ Partner Site Tübingen, German Cancer Consortium, Germany
https://doi.org/10.7554/eLife.40126
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Cite this article
  1. F Tudor Ilca
  2. Andreas Neerincx
  3. Clemens Hermann
  4. Ana Marcu
  5. Stefan Stevanović
  6. Janet E Deane
  7. Louise H Boyle
(2018)
TAPBPR mediates peptide dissociation from MHC class I using a leucine lever
eLife 7:e40126.
https://doi.org/10.7554/eLife.40126
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Abstract

Tapasin and TAPBPR are known to perform peptide editing on major histocompatibility complex class I (MHC I) molecules; however, the precise molecular mechanism(s) involved in this process remain largely enigmatic. Here, using immunopeptidomics in combination with novel cell-based assays that assess TAPBPR-mediated peptide exchange, we reveal a critical role for the K22-D35 loop of TAPBPR in mediating peptide exchange on MHC I. We identify a specific leucine within this loop that enables TAPBPR to facilitate peptide dissociation from MHC I. Moreover, we delineate the molecular features of the MHC I F pocket required for TAPBPR to promote peptide dissociation in a loop-dependent manner. These data reveal that chaperone-mediated peptide editing on MHC I can occur by different mechanisms dependent on the C-terminal residue that the MHC I accommodates in its F pocket and provide novel insights that may inform the therapeutic potential of TAPBPR manipulation to increase tumour immunogenicity.

https://doi.org/10.7554/eLife.40126.001

Introduction

Major histocompatibility complex class I (MHC I) molecules play a critical role in immmunosurveillance, particularly in the context of viral infections and cancer, by presenting antigenic peptides to CD8 +T cells. Prior to their cell surface export, MHC I molecules undergo peptide editing, a process that involves the exchange of low-affinity peptides for those of higher affinity. In addition to ensuring that only stable peptide:MHC I complexes are released to the plasma membrane, peptide editing ultimately controls the peptide repertoire that is displayed for immune detection. For over two decades, tapasin was the only known peptide editor for MHC I, facilitating peptide selection within the confines of the peptide loading complex (PLC) (Williams et al., 2002; Howarth et al., 2004; Chen and Bouvier, 2007; Wearsch and Cresswell, 2007). However, it is now well-recognised that the tapasin-related protein TAPBPR is a second independent peptide editor that performs peptide exchange outside the PLC (Boyle et al., 2013; Hermann et al., 2013; Hermann et al., 2015; Morozov et al., 2016). Furthermore, TAPBPR can work in cooperation with UDP-glucose:glycoprotein glucosyltransferase 1 (UGT1) to reglucosylate MHC I, causing recycling of MHC molecules to the PLC (Neerincx et al., 2017).

Although TAPBPR usually functions as an intracellular peptide editor, we have recently made the fascinating discovery that when given access to surface expressed MHC I molecules, TAPBPR retains its function as a peptide exchange catalyst and can be utilised to display immunogenic peptides of choice directly onto the surface of cells (Ilca et al., 2018). We have therefore identified that manipulation of TAPBPR function may be utilised as a potential immunotherapeutic that facilitates the presentation of both neoantigens and viral-derived peptides, thereby overriding the endogenous cellular antigen processing pathway. Moreover, we have also developed two novel functional assays that enable detailed interrogation of TAPBPR-mediated peptide exchange on MHC I (Ilca et al., 2018).

Precisely how tapasin and TAPBPR function at the molecular level remains largely enigmatic. The recently reported crystal structures of human TAPBPR in complex with mouse MHC I captured the endpoint of peptide editing, thereby suggesting that TAPBPR facilitates peptide exchange by widening the peptide binding groove of MHC I at the α2–1 region (Jiang et al., 2017; Thomas and Tampé, 2017). However, there remains an incomplete understanding of the step-by-step processes by which the two peptide editors, TAPBPR and tapasin, recognise peptide-loaded MHC I molecules and actively facilitate peptide dissociation to result in the conformations observed in the crystal structures. Indeed, McShan et al. have recently used NMR in an attempt to further delineate the dynamic process of peptide exchange on MHC I by TAPBPR (McShan et al., 2018).

Intriguingly, the structure reported by Thomas and Tampe identified a loop of TAPBPR that was proposed to interact with the peptide binding groove of MHC I, where the C-terminus of bound peptide usually resides (Thomas and Tampé, 2017). While this study demonstrated the localisation of the loop, to date, there is no experimental evidence to support the notion that this loop mediates peptide exchange on MHC I. In contrast, Jiang et al. failed to capture the loop in proximity to the peptide-binding groove (Jiang et al., 2017), further questioning the relevance and importance of this loop in TAPBPR-mediated peptide exchange. Given the discordance between the data reported for the captured structures and the lack of functional evidence to support any role for this loop, it is vital to reconcile these discrepancies to understand whether the TAPBPR loop is involved in peptide exchange.

Here, we investigate the functional importance of the K22-D35 loop using two newly developed assays in combination with immunopeptidomic analysis. Our data demonstrates that this loop is critical for peptide dissociation from MHC I. Furthermore, we highlight key molecular features governing TAPBPR:MHC I interaction and provide insight into the mechanism(s) of peptide selection on MHC I molecules.

Results

The TAPBPR K22-D35 loop lies at the interface with the MHC I peptide binding groove

Prior to the recent determination of the TAPBPR-MHC I crystal structures (Jiang et al., 2017; Thomas and Tampé, 2017), we docked our model of TAPBPR onto a previously determined structure of HLA-A2, using our mutagenesis data that identified critical regions in the TAPBPR-MHC I interface (Hermann et al., 2013). Our docking identified a region of TAPBPR that lies close to the peptide binding groove of MHC I, in the proximity of the F pocket (Figure 1a, dotted circle). This region contained a loop that differs between tapasin and TAPBPR. In tapasin, this loop appears to be rather short and is not sufficiently well ordered in the crystal structure to be visible (Dong et al., 2009), while in TAPBPR this loop is significantly longer (Figure 1b). The two crystal structures of the TAPBPR-MHC I complex support our prediction regarding the arrangement of TAPBPR relative to MHC I, including this loop region being very near the F pocket (Figure 1c). However, the position and orientation of the loop in the structures is poorly defined. In the structure from Jiang et al. (2017), this loop is not sufficiently well ordered to be modelled. In the structure from Thomas and Tampé (2017), the loop has been modelled; however, the electron density into which it was built is not well defined: several side chains and even several of the backbone atoms do not fit the electron density well (Figure 1d). It is likely that alternative orientations of the loop would also satisfy the crystallographic data presented. Therefore, it is critically important to verify whether there is any functional role of this loop in peptide editing.

Figure 1
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TAPBPR loop interactions with MHC I.

(a) Model of TAPBPR (pink) docked onto MHC I (blue) and β2m (cyan) based on interaction studies (Hermann et al., 2013). (b) Top panel, illustration of the proximity of the TAPBPR loop region to the peptide binding groove (viewed from the top of complex shown in panel a). Lower panel, schematic diagrams of the amino acid composition of the TAPBPR and tapasin loops compared to the length and orientation of a peptide. (c) Overlay of two recent X-ray structures of TAPBPR in complex with MHC I (Jiang et al., 2017; Thomas and Tampé, 2017) (PDB ID 5WER and 5OPI) oriented and coloured to illustrate the similarity to our TAPBPR:MHC I complex (panel a). The position of the TAPBPR loop is circled (black dashed line). (d) The electron density map (2Fo-Fc, green mesh) and the built model (maroon sticks, residues D23-E34) are shown for the loop region of TAPBPR (PDB ID 5OPI). Two views of the loop and density are shown rotated by 90 degrees. (e) Expression of TAPBPR loop variants in IFNγ treated HeLaM-TAPBPRKO and their interaction with MHC I and UGT1. Western blotting for calnexin is included as a loading control. Representative of three independent experiments.

https://doi.org/10.7554/eLife.40126.002

TAPBPR loop mutants are stably expressed and bind MHC I and UGT1

To test whether the K22-D35 loop of TAPBPR was a functionally important region for mediating peptide selection, we first replaced all the residues in the loop with either glycine, alanine or serine, to produce a TAPBPR variant with a potentially functionless loop (TAPBPRØloop) (Table 1). More subtle mutations of this loop were designed based on the work by Springer and colleagues, who demonstrated that dipeptides carrying long hydrophobic residues are able to bind to the peptide binding groove of recombinant MHC I and enhance peptide dissociation (Saini et al., 2015). Thus, we explored whether a leucine residue at position 30 of the mature TAPBPR protein, the only long hydrophobic residue within the entire loop, was involved in peptide exchange on MHC I. We created two TAPBPR variants in order to test this. First, we replaced the leucine with glycine in the TAPBPRWT molecule (TAPBPRL30G). Second, we reintroduced leucine 30 into TAPBPR with the dysfunctional loop (TAPBPRØG30L) (Table 1). Upon transduction into TAPBPR-deficient HeLaM cells (HeLaM-TAPBPRKO), steady state expression of all the TAPBPR loop mutants was similar to TAPBPRWT and all variants interacted equally well with both MHC I and UGT1 (Figure 1e), suggesting the overall protein stability and structure of TAPBPR was not significantly affected by the changes to the loop.

Table 1
Panel of TAPBPR loop mutants. 

Residues altered are highlighted in red.

https://doi.org/10.7554/eLife.40126.003
TAPBPR variantLoop sequence
WTKDGAHRGALASSED
ØloopAAGGSGGGGSGGAA
L30GKDGAHRGAGASSED
ØG30LAAGGSGGGLGGGAA

The K22-D35 loop is essential for mediating peptide exchange on HLA-A*68:02

Recently, we have developed two novel assays which can be used to measure TAPBPR-mediated peptide exchange on MHC I molecules. The first assay established takes advantage of the small proportion of TAPBPR that escapes to the cell surface upon its over-expression in cell lines (Ilca et al., 2018). We have demonstrated that plasma membrane expressed wild-type TAPBPR efficiently mediates peptide exchange on cell surface HLA-A*68:02 molecules found on HeLaM cells (Ilca et al., 2018). We have also previously shown that TAPBPR-mediated peptide exchange in this assay occurs directly on the cell surface, given that it works on cells incubated at 4°C, which inhibits membrane trafficking (Ilca et al., 2018). To initially explore the functional importance of the K22-D35 loop, we tested whether plasma membrane expressed TAPBPRØloop was capable of mediating peptide exchange on HLA-A*68:02 to a similar extent as TAPBPRWT. When exploring the ability of TAPBPR to promote peptide association, we found plasma membrane expressed TAPBPRØloop enhanced the binding of an exogenous fluorescent peptide specific for HLA-A*68:02 (YVVPFVAK*V) onto cells to a similar extent as TAPBPRWT (Figure 2a). This may be due, in part, to TAPBPR enhancing the trafficking of associated, peptide-receptive MHC I with it through the secretory pathway. However, when we tested the ability of TAPBPR to mediate peptide dissociation (i.e. removal of the bound fluorescent peptide in the presence of an unlabelled competitor peptide) (Figure 2b), in contrast to the efficient exchange observed with TAPBPRWT, very little, if any, dissociation of fluorescent peptide was observed with TAPBPRØloop (Figure 2c–2e). This suggests that the K22-D35 loop of TAPBPR is essential for mediating efficient peptide dissociation from HLA-A*68:02.

Figure 2 with 1 supplement see all
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The TAPBPR K22-D35 loop is critical for peptide exchange.

(a) Typical peptide binding when cells gated for expressing high levels of surface TAPBPR were incubated with 10 nM YVVPFVAK*V peptide for 15 min at 37°C on IFNγ treated HeLaM-TAPBPRKO cells -/+expression of TAPBPRWT, TAPBPRØloop, TAPBPRL30G or TAPBPRØG30L. (b) Schematic representation of the experimental workflow used to compare the efficiency of peptide exchange by plasma membrane bound TAPBPRWT with the plasma membrane bound TAPBPR loop mutants. (c) Histograms show the level of dissociation of YVVPFVAK*V (YVV*) in the absence (blue line) and presence of 100 nM non-labelled competitor peptide YVVPFVAKV (YVV)(orange line) or EGVSKQSNG (ETVΔ2/9), a peptide in which the anchors which permit HLA-A*68:02 binding are mutated to produce a non-binding derivative (black line). Similar patterns of dissociation were found on cells incubated at 4°C demonstrating that the peptide exchange occurs directly on the cell surface (see Figure 2—figure supplement 1). (d-e) Graphs show the percentage of fluorescent peptide YVVPFVAK*V (YVV*) remaining in the presence of (d) 100 nM or (e) increasing concentrations of the non-labelled competitor peptide YVVPFVAKV as a percentage of the bound YVVPFVAK*V observed in the absence of competitor peptide from four independent experiments. Error bars show -/+SD.****p≤0.0001, ***p≤0.001 using unpaired two-tailed t-tests.

https://doi.org/10.7554/eLife.40126.004

L30 is a critical residue of TAPBPR for peptide exchange on HLA-A*68:02

Next, we tested the ability of both plasma membrane bound TAPBPRL30G and TAPBPRØG30L to promote peptide exchange in our assay. As observed above with TAPBPRØloop, plasma membrane expressed TAPBPRL30G and TAPBPRØG30L were able to promote the association of exogenous fluorescent peptide onto cells (Figure 2a). However, when we explored the ability of TAPBPRL30G to mediate fluorescent peptide dissociation from HLA-A*68:02, we found that it was incapable of mediating efficient peptide dissociation in the presence of unlabelled competitor (Figure 2c–2e). Strikingly, alteration of this single residue had the same effect on the function of TAPBPR as mutating the entire loop. The crucial role of L30 in mediating peptide dissociation was further supported with our observation that TAPBPRØG30L, in which the leucine residue alone is restored into the dysfunctional TAPBPRØloop molecule, transformed it into a functioning peptide exchange catalyst on HLA-A*68:02, albeit at reduced capability compared to TAPBPRWT (Figure 2c–2e). While TAPBPRWT efficiently promoted rapid peptide exchange at very low concentrations of competitor peptide, both TAPBPRØloop and TAPBPRL30G were extremely inefficient at mediating peptide exchange on HLA-A*68:02 molecules, even in the presence of high concentrations of competitor peptide (Figure 2e). These results suggest that L30 is a critical residue within the loop of TAPBPR for mediating peptide dissociation from HLA-A*68:02.

Soluble TAPBPR lacking L30 cannot facilitate peptide dissociation from HLA-A*68:02

We have also recently shown that soluble TAPBPR, consisting only of the lumenal domains of the wild-type molecule (i.e. lacking its transmembrane region and cytoplasmic tail) can also efficiently promote peptide exchange on three MHC I molecules: HLA-A*68:02, HLA-A2 and H-2Kb (Ilca et al., 2018). Therefore, we can also use soluble TAPBPR as a second means to assay the ability of TAPBPR mutants to mediate efficient peptide exchange on MHC I. Using this approach, the majority of MHC I molecules that TAPBPR will have access to will be folded with bound peptides of relativity high affinity expressed on the surface of cells. The lumenal domains of the TAPBPR variants with C-terminal His-tags were purified using Ni-affinity chromatography from the culture supernatants of transfected 293T cells (Figure 3a). Differential scanning fluorimetry revealed all TAPBPR loop variants had a similar melting temperature as TAPBPRWT (Figure 3b), indicating the alterations made to the loop had not significantly affected protein folding and stability. Comparison of the ability of the soluble TAPBPR variants to mediate peptide exchange on HLA-A*68:02 molecules on HeLaM cells revealed that TAPBPRWT was most efficient, followed by TAPBPRØG30L, which exhibited ~33% peptide exchange activity relative to TAPBPRWT (in the presence of 10 nM ETVSK*QSNV) (Figure 3c and d). However, both TAPBPRL30G and TAPBPRØloop were unable to efficiently mediating peptide exchange, displaying only ~3% of the exchange activity of TAPBPRWT (in the presence of 10 nM ETVSK*QSNV) (Figure 3c and d). As previously shown (Ilca et al., 2018), soluble TAPBPRTN5, a mutant which cannot bind to MHC I, did not mediate any peptide exchange (Figure 3c and d). This hierarchy (WT>ØG30L>L30G>TN5) was maintained over a wide range of exogenous TAPBPR concentrations (Figure 3e). Furthermore, the same hierarchical order of peptide exchange efficiency for the variants was observed using another HLA-A*68:02-binding peptide, YVVPFVAK*V (Figure 3c & d).

Figure 3
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Soluble TAPBPR loop variants exhibit reduced ability to mediate peptide exchange on surface HLA-A*68:02 molecules.

(a) Expression and purity of soluble forms of WT, L30G, ØG30L, and Øloop TAPBPR variants after their purification from the culture supernatant using Ni-affinity. (b) Differential scanning fluorimetry demonstrates the three TAPBPR loop mutants have equivalent thermal denaturation profiles as TAPBPRWT. (c) Histograms of the typical fluorescent peptide binding to IFNγ-treated HeLaM cells incubated -/+100 nM exogenous soluble TAPBPR variant for 15 min at 37°C, followed by incubation with 10 nM ETVSK*QSNV or YVVPFVAK*V for an additional 15 min. TAPBPRTN5, in which isoleucine at position 261 is mutated to lysine, to produce a TAPBPR variant which cannot bind to MHC I, is included as a control (d) Bar graphs show the reproducibility of results in (c). (e) Dose response curves of fluorescent peptide binding to IFN-γ treated HeLaM cells incubated with increasing concentrations of the soluble TAPBPR variants prior to the addition of 10 nM ETVSK*QSNV. Error bars represent MFI -/+SD from four independent experiments. ****p≤0.0001 using unpaired two-tailed t-tests.

https://doi.org/10.7554/eLife.40126.006

The K22-D35 loop is essential for soluble TAPBPR to bind peptide-loaded MHC I

When we determined the ability of the soluble TAPBPR variants to bind to HLA-A*68:02, TAPBPRWT was the most efficient binder, followed by TAPBPRØG30L (Figure 4a). However, TAPBPRL30G and TAPBPRØloop were unable to make productive interactions with surface expressed HLA-A*68:02 (Figure 4a). As expected, TAPBPRTN5, which has a disrupted binding site for MHC I, was unable to bind to cells (Hermann et al., 2013) (Figure 4a). As this inability of soluble TAPBPRØloop and TAPBPRL30G to interact with MHC I contradicted our finding with their membrane-bound counterparts (Figure 1e), we determined whether these soluble TAPBPR variants could bind to the total cellular MHC I from cell lysates in pull-down experiments. All soluble TAPBPR variants (with the exception of TAPBPRTN5) were capable of binding significant amounts of MHC I, although less MHC I was detected bound to TAPBPRL30G and TAPBPRØloop (Figure 4b). A major difference between surface MHC I on intact cells (Figure 4a) and total cellular MHC I in detergent (Figure 4b) is the availability of peptide-receptive molecules. Therefore, we speculated that TAPBPR molecules with alterations to the loop are still able to bind to peptide-receptive MHC I but are unable to physically make MHC I peptide-deficient due to their inability to dissociate peptide. Consistent with this, incubation of cells at 26°C, which increases the expression of peptide-receptive MHC I on the plasma membrane (Ljunggren et al., 1990; Schumacher et al., 1990; Garstka et al., 2015), resulted in the TAPBPR loop variants, but not TAPBPRWT, exhibiting increased binding to cells (Compare Figure 4c with Figure 4a summarised in Figure 4d). While no significant change was observed in the ability of exogenous TAPBPRWT to bind to cells incubated at the lower temperature, a ~ 7 fold increase in binding of TAPBPRL30G and TAPBPRØloop was observed at 26°C compared to 37°C, and the binding of TAPBPRØG30L increased by ~2.5 fold (Figure 4d). When given access to surface expressed peptide-receptive MHC I upon incubation at 26°C, all soluble TAPBPR variants exhibited a corresponding increase in the ability to mediate peptide exchange (compare Figure 3c (37°C) with Figure 4e (26°C), summarised in Figure 4f). These findings suggest that TAPBPR variants lacking L30 are indeed able to bind to peptide-receptive MHC I but are unable to physically make MHC I peptide-deficient due to their inability to efficiently facilitate peptide dissociation. Consistent with this, when cells cultured at 26°C were incubated with an HLA-A*68:02-binding peptide prior to testing soluble TAPBPR binding, the peptide significantly reduced the ability of TAPBPRØloop to bind to cells, while the binding of TAPBPRWT was unaffected (Figure 4—figure supplement 1).

Figure 4 with 1 supplement see all
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Residues K22-D35 are essential for soluble TAPBPR to bind peptide-loaded MHC I.

(a and c) Histograms of soluble TAPBPR loop variant binding to HeLaM-HLA-ABCKO cells expressing HLA-A*68:02 incubated with 100 nM TAPBPR at (a) 37°C or (c) 26°C for 30 min. TAPBPRTN5, a TAPBPR variant which cannot bind to MHC I, is included as a negative control. (b) TAPBPR pull-downs on IFNγ-treated HeLaM-TAPBPRKO cells incubated with soluble TAPBPR loop mutants reveal all variants are capable of binding to MHC I, but do not bind to UGT1. TAPBPRTN5 is included as a non-MHC binding control. Data is representative of three independent experiments. (d) Bar graph comparing soluble TAPBPR variant binding to HeLaM-HLA-ABCKO+A*68:02 cells at 37°C with 26°C from three independent experiments. Error bars represent -/+SD. (e) Histograms show typical fluorescent peptide binding to IFNγ induced HeLaM cells treated -/+100 nM soluble TAPBPR variants for 15 min at 26°C, followed by incubation with 10 nM ETVSK*QSNV for 15 min at 26°C. (f) Bar graph compares ETVSK*QSNV peptide binding to HeLaM cells treated -/+ soluble TAPBPR variants at 37°C with 26°C from three independent experiments. Error bars represent -/+SD. n/s = not significant, *p≤0.05, **p≤0.01, ***p≤0.001, ****p≤0.0001, using unpaired two-tailed t-tests.

https://doi.org/10.7554/eLife.40126.007

Mutation of the TAPBPR K22-D35 loop alters the peptide repertoire presented on MHC I

Having shown a role for the K22-D35 loop of TAPBPR in mediating efficient peptide dissociation from MHC I, we next determined whether the peptide repertoire presented by MHC I molecules was altered in cells upon mutation of the loop. When we compared the immunopeptidomes of IFNγ-treated HeLaM-TAPBPRKO cells expressing TAPBPRWT with cells expressing TAPBPRØloop, we found significant changes in the peptides presented on MHC I (Figure 5a). 461 peptides were found exclusively in the TAPBPRWT-expressing cells and 550 peptides were found exclusively in the cells expressing the Øloop variant (Figure 5a). Label-free quantitation by mass spectrometry revealed that there were also significant changes in the abundance of some peptides between TAPBPRWT and TAPBPRØloop, with 193 peptides exhibiting increased abundance in cells expressing TAPBPRWT (Red circles, Figure 5b) and 222 peptides displaying increased abundance in TAPBPRØloop-expressing cells (Blue circles, Figure 5b). These findings demonstrate that there are significant changes in the peptide repertoire presented on MHC I upon mutation of the TAPBPR loop. While there were still large differences in the peptide repertoires between TAPBPRWT and TAPBPRØG30L-expressing cells, based on a presence/absence approach (Figure 5a), restoration of the leucine residue into the loop appeared to somewhat reduce some of the changes observed in peptide abundance (Figure 5b). Assignment of the identified peptides to the MHC I allotype found in HeLaM cells revealed a similar HLA distribution between the various loop mutants (Figure 5c and d).

Figure 5 with 4 supplements see all
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Mutation of the K22-D35 loop of TAPBPR changes the peptide repertoire presented on cells.

Peptides eluted from W6/32-reactive MHC I complex isolated from IFNγ treated HeLaM-TAPBPRKO expressing either TAPBPRWT, TAPBPRØloop or TAPBPRØG30L were analysed using LC-MS/MS. In dataset 1 (a–d), cells were frozen immediately post-trypsination while in dataset 2 (e–h) cells were allowed to recover in media for 30 min after trypsination, before freezing. The sequences of identified peptides are listed in Figure 5—source datas 2–7. The comparison of all five technical replicates for the two datasets is shown in Figure 5—figure supplement 1. (a,e) Venn diagrams compare all the identified peptides using a presence/absence approach. (b,f) Volcano plots graphically summarise label-free quantitation, displaying modulated peptides between two cells lines. Colour circles highlight the peptide which are differentially expressed between two cell lines after applying an adjusted p-value of <0.01. The list of these peptides is available in Figure 5—source datas 8 and 9. n = number of significantly modulated peptides, % demonstrates the fraction of significantly modulated peptides in a specific cell line compare to all peptides in the comparison. (c,d,g,h) Bar graphs summarise the MHC I molecules (HLA-A*68:02, -B*15:03 or –C*12:03) that the (c,g) identified peptides in a/e, and (d,h) the significantly modulated peptides identified in b/f were matched to using the NetMHCpan-4.0. In (c) and (g), peptides not successfully assigned are indicated in orange (rest). Analysis of the peptide repertoire from a further TAPBPR-loop mutant lacking L30 and from a third biological repeat can be found in Figure 5—figure supplements 2 and 3 respectively. Analysis of the predicted affinity of peptides differential modulated upon mutation of the loop (i.e those in b) and (f) can be found in Figure 5—figure supplement 4.

https://doi.org/10.7554/eLife.40126.009
Figure 5—source data 1

Binding of TAPBPR to the individual HLA molecules found in HeLaM cells.

Soluble TAPBPRWT or soluble TAPBPRØloop were incubated with the indicated LABScreen single antigen HLA class I beads (from One Lambda, Canoga Park, California) for 60 min at RT. After washing 3 times in PBS, bound TAPBPR was detected using the TAPBPR-specific mAb PeTe4 and a goat anti-mouse PE-conjugated secondary antibody. Samples were analysed using the Luminex Fluoroanalyser system. The table shows the MFI of PeTe4 staining ± SD for the indicated HLA beads incubated in the absence and presence of 1 µM of TAPBPRWT or TAPBPRØloop. The expression of MHC I (detected using W6/32) provided by One Lambda for the specific lot of LABScreen beads used is included as a control.

https://doi.org/10.7554/eLife.40126.014
Figure 5—source data 2

Dataset 1 - peptides eluted from W6/32-reactive MHC I complexes from IFNγ treated HeLaM-TAPBPRKO cells expressing TAPBPRWT.

https://doi.org/10.7554/eLife.40126.015
Figure 5—source data 3

Dataset 1 - peptides eluted from W6/32-reactive MHC I complexes from IFNγ treated HeLaM-TAPBPRKO cells expressing TAPBPRØloop.

https://doi.org/10.7554/eLife.40126.016
Figure 5—source data 4

Dataset 1 - peptides eluted from W6/32-reactive MHC I complexes from IFNγ treated HeLaM-TAPBPRKO cells expressing TAPBPRØG30L.

https://doi.org/10.7554/eLife.40126.017
Figure 5—source data 5

Dataset 2 - peptides eluted from W6/32-reactive MHC I complexes from IFNγ treated HeLaM-TAPBPRKO cells expressing TAPBPRWT.

https://doi.org/10.7554/eLife.40126.018
Figure 5—source data 6

Dataset 2 - peptides eluted frm W6/32-reactive MHC I complexes from IFNγ treated HeLaM-TAPBPRKO cells expressing TAPBPRØloop.

https://doi.org/10.7554/eLife.40126.019
Figure 5—source data 7

Dataset 2 - peptides eluted from W6/32-reactive MHC I complexes from IFNγ treated HeLaM-TAPBPRKO cells expressing TAPBPRØG30L.

https://doi.org/10.7554/eLife.40126.020
Figure 5—source data 8

Dataset 1 - analysis of eluted peptides used to generate volcano plots.

https://doi.org/10.7554/eLife.40126.021
Figure 5—source data 9

Dataset 2 - analysis of eluted peptides used to generate volcano plots.

https://doi.org/10.7554/eLife.40126.022
Figure 5—source data 10

Peptides eluted from W6/32-reactive MHC I complexes from IFNγ treated HeLaM-TAPBPRKO cells expressing TAPBPRM29.

https://doi.org/10.7554/eLife.40126.023
Figure 5—source data 11

Dataset 3 - peptide list for third biological repeat for TAPBPRWT expressing cells.

https://doi.org/10.7554/eLife.40126.024
Figure 5—source data 12

Dataset 3 - peptide list for third biological repeat for TAPBPRØloop expressing cells.

https://doi.org/10.7554/eLife.40126.025
Figure 5—source data 13

Dataset 3 - peptides list for third biological repeat for TAPBPRØG30L expressing cells.

https://doi.org/10.7554/eLife.40126.026

Given the ability of surface expressed TAPBPRWT, but not of TAPBPRØloop, to mediate peptide dissociation from surface MHC I molecules, as identified in Figure 2, we performed an additional experiment in which the cells were incubated at 37°C in media after harvesting to permit the surface expressed TAPBPR (~5% of the total TAPBPR pool in these cells [Ilca et al., 2018]) to perform peptide dissociation/exchange on surface expressed MHC I molecules, prior to performing immunopeptidomic analysis. While this experiment confirmed the significant changes in the peptide repertoire presented on MHC I upon mutation of the TAPBPR loop (Figure 5e and f), it also surprisingly revealed a large effect on the peptides assignable to HLA-A*68:02 between cells expressing TAPBPRWT and TAPBPRØloop (Figure 5g and h). Based on a presence/absence approach, only 29% in TAPBPRWT were now assignable to HLA-A*68:02, compared to 37% for cell expressing TAPBPRØloop-expressing cells (which was similar to the results found when the immunopeptidomics was performed immediately after cell harvesting [Figure 5d]). Similarly, abundance analysis revealed >80% of the up-modulated peptides in TAPBPRØloop-expressing cells belonged to HLA-A*68:02, compared to only ~20% of the up-modulated peptides in TAPBPRWT (Figure 5h). In keeping with this, we observed similar alterations in HLA-A*68:02-assignable peptides when the leucine residue was restored into the dysfunctional loop (Figure 5g and h), as the ones observed for TAPBPRWT. This suggests that TAPBPR with a functional loop is preferentially stripping a proportion of peptides from HLA-A*68:02 molecules, but not from the HLA-B or -C molecules found on HeLaM cells. To explore this further, we compared the ability of soluble TAPBPR to bind to soluble heterotrimeric HLA-A*68:02, -B*15:03 or -C*12:03 molecules coupled to beads. This revealed a strong interaction between TAPBPRWT and HLA-A*68:02 (Figure 5—source data 1). However, soluble TAPBPRWT failed to bind to heterotrimeric HLA-B*15:03 and –C*12:03 molecules (Figure 5—source data 1). This preferential association of TAPBPR with peptide-loaded HLA-A*68:02 over the other MHC I found in HeLaM cells likely explains why we only observe a loss of HLA-A*68:02 peptides in cells expressing TAPBPR with a functional loop. Interestingly, this analysis of soluble HLA molecules coupled on beads also confirmed the considerable reduction in binding of TAPBPR to HLA-A*68:02 upon mutation of the loop (Figure 5—source data 1). Taken together, this data demonstrates that the loop is involved in selecting MHC I peptides within cells and highlights the importance of leucine 30 in the peptide selection process, particularly for HLA-A*68:02 molecules.

L30 enables TAPBPR to mediate peptide exchange on MHC I molecules that accommodate hydrophobic amino acids in their F pocket

Given the proximity of the TAPBPR loop to the F pocket of MHC I (Figure 1) and that the L30 residue of TAPBPR was both necessary and sufficient for efficient peptide exchange on HLA-A*68:02 (Figure 2 and 3), we hypothesised that the TAPBPR loop facilitates peptide dissociation by binding into the F pocket, thereby competing with the C-terminus of the peptide. If so, this competitive binding would only be possible for MHC I molecules that could accommodate leucine or similar hydrophobic residues in the F pocket. With this in mind, we explored the importance of the TAPBPR K22-D35 loop in mediating peptide exchange on two other MHC I molecules, HLA-A*02:01 and H-2Kb, which naturally accommodate hydrophobic amino acids in their F pocket like HLA-A*68:02. Compared to HLA-A*68:02, HLA-A*02:01 accommodates very similar anchor residues in both B and F pockets, whereas H-2Kb has a completely different binding motif, with the exception that it binds a similar anchor residue in the F pocket (Figure 6a). Thus, given that both HLA-A*02:01 and H-2Kb could potentially accommodate L30 of the TAPBPR loop in their F-pocket, we predicted TAPBPR-mediated peptide editing on these two additional MHC I would also be dependent on L30.

Figure 6 with 1 supplement see all
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F pocket specificity for hydrophobic residues influences the ability of TAPBPR to edit peptides in a loop-dependent manner.

(a) Comparison of the A-F pocket specificities of the HLA-A*68:02, HLA-A*02:01, H-2Kb and HLA-A*68:01 peptide binding grooves. (b) Binding of fluorescent peptide to IFNγ-treated HeLaM-HLA-ABCKO transduced with HLA-A*02:01, mouse EL4 cells (which express H-2Kb) or HeLaM-HLA-ABCKO transduced with HLA-A*68:01 -/+1 µM soluble TAPBPR variant for 15 min at 37°C, followed by incubation with 10 nM NLVPK*VATV (HLA-A2 binding peptide) for 60 min, 1 nM SIINFEK*L (H-2Kb binding peptide) for 30 min or 100 nM KTGGPIYK*R (HLA-A*68:01) for 60 min at 37°C. (c) Bar graph summarising the peptide exchange by soluble TAPBPR variants as performed in b). Error bars represent MFI -/+ SD from four independent experiments. ****p ≤ 0.0001, ***p ≤ 0.001, *p ≤ 0.05, using unpaired two-tailed t-tests. (d) Structure of MHC I from above the binding groove and the different amino acids between HLA-A*68:02 and –A*68:01 highlighted in red.

https://doi.org/10.7554/eLife.40126.027

As observed above with HLA-A*68:02, we found that TAPBPRWT was the most efficient catalyst on both HLA-A*02:01 and H-2Kb, followed by TAPBPRØG30L, while both TAPBPRL30G and TAPBPRØloop were least efficient (Figure 6b and c). For example, the Øloop, L30G, and ØG30L variants exhibited ~23%, 32% and 54% activity relative to TAPBPRWT, respectively, when measuring NLVPK*VATV binding onto HLA-A2 introduced into HeLaM-HLA-ABCKO cells by transduction (Figure 6c). We observed similar trends using another HLA-A2 binding peptide, YLLEK*LWRL, on this cell line, as well as when testing peptide binding to HLA-A2 molecules expressed on MCF-7 cells (Figure 6—figure supplement 1). For SIINKEK*L binding to H-2Kb expressed on EL4 cells, the Øloop, L30G, and ØG30L variants exhibited ~30%, 31%, 85% activity relative to TAPBPRWT, respectively (Figure 6c). Thus, although TAPBPR can still mediate peptide exchange on HLA-A2 and H-2Kb in the absence of the loop to some extent, the L30 residue in the K22-D35 loop of human TAPBPR plays a critical role in promoting efficient peptide exchange on HLA-A*68:02, HLA-A2 and H-2Kb. The shared dependency on L30 to enable TAPBPR to efficiently mediate peptide exchange on both H-2Kb and HLA-A*68:02 is remarkable, considering the low degree of similarity between these two MHC I, both in their amino acid sequences and in their binding motifs. However, the key shared feature between these MHC I is that both accommodate hydrophobic residues in their F pocket.

TAPBPR cannot use the loop to efficiently mediate peptide exchange on MHC I molecules with F pocket specificities for non-hydrophobic amino acids

We next tested the role of the TAPBPR loop in catalysing peptide exchange on an MHC I molecule that does not accommodate a hydrophobic residue in its F pocket. In order to only explore the contributing role of the F pocket, with minimal other polymorphisms, we chose HLA-A*68:01, since it only differs from HLA-A*68:02 by five amino acids (Niu et al., 2013). Most of the differences between these two HLA are residues dictating the specificity of the F pocket for the C-terminal peptide anchor residue. Hence, while both molecules have a similar anchor residue in their B pocket, the F pocket of HLA-A*68:02 accommodates an aliphatic residue, whereas the F pocket of HLA-A*68:01 accommodates a basic residue (Figure 6a) (Niu et al., 2013). Strikingly, in contrast to the three MHC I molecules tested previously (which all bind hydrophobic residues in their F pocket), there was a significant impairment in the ability of TAPBPRWT to load peptides onto HLA-A*68:01 (Figure 6b and c). Moreover, there was no significant difference in the ability of TAPBPR to exchange peptides on HLA-A*68:01 upon mutation of the loop, with the different loop variants all exhibiting a similar ability as soluble TAPBPRWT to load the fluorescent peptide KTGGPIYK*R onto HLA-A*68:01 expressed on HeLaM-HLA-ABCKO cells (Figure 6b and c). Thus, in contrast to HLA-A*68:02, which is extremely receptive to TAPBPR-mediated peptide exchange in a loop-dependent manner, HLA-A*68:01 is significantly less responsive to TAPBPR-mediated peptide exchange, which only occurs in a loop-independent manner. Therefore, the five amino acid differences between these two HLA molecules, three of which are around the F pocket (Figure 6d), strongly influence the receptivity of these two MHC I molecules to TAPBPR-mediated peptide exchange, the efficiency of which appears to be strongly influenced by the ability of the K22-D35 loop (specifically L30) to interact with the peptide binding groove. These findings support the concept that the L30 residue of TAPBPR is capable of binding into the F pocket of MHC I in order promote the dissociation of the bound peptide in a competitive manner.

Mutation of residue 116 in the MHC I F pocket alters TAPBPR binding

Our data thus far is consistent with the L30 residue of the TAPBPR loop binding to the F pocket of MHC I molecules which accommodate hydrophobic residues. One residue that differs between HLA-A*68:02 and -A*68:01 and was reported to be crucial in determining the F pocket specificity of MHC I for peptide residues is the one on position 116 (Sidney et al., 2008). The impact of residue 116 on the F pocket architecture is well highlighted in the crystal structures of peptide-bound HLA-A*68:02 and HLA-A*68:01 (Niu et al., 2013). Namely, HLA-A*68:02 contains a tyrosine on position 116, whereas HLA-A*68:01 has an aspartate (Figure 7a). As captured in the two structures, D116 of HLA-A*68:01 forms strong dipole interactions with both residue R114 of the groove and with the arginine on position 9 of the peptide, determining a strong preference of the HLA-A*68:01 F pocket for basic anchor residues. In contrast, residue Y116 of HLA-A*68:02 does not form any interaction with H114, keeping the hydrophobic patches of the F pocket in an open conformation, which allows it to accommodate hydrophobic peptide residues (Figure 7a). We believe that this is the reason why the L30 residue of the TAPBPR loop has access to the F pocket of HLA-A*68:02, but not to the one of HLA-A*68:01.

Figure 7 with 1 supplement see all
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Mutation of the MHC I F pocket alters TAPBPR-mediated peptide editing.

(a) PyMOL images of the binding grooves of HLA-A*68:02 (PDB ID 4HX1) and -A*68:01 (PDB ID 4HWZ), with residues found at position 116 and 114 highlighted. (b) Histograms show the surface expression of HLA-A*68:02WT, -A*68:02Y116D, -A*68:01WT and -A*68:01D116Y, detected using W6/32, when expressed in HeLa-M-HLA-ABCKO cells. (c, e, g) HeLaM-HLA-ABCKO cells expressing the panel of HLA-A*68 molecules were incubated with 1 μM of soluble TAPBPR for 15 min at 37°C, followed by either (c) detection of surface bound TAPBPR using PeTe4, or incubation with (e) 10 nM ETVSK*QSNV (ETV*) or (g) 100 nM KTGGPIYK*R (KTG*) peptide for 15 min and 60 min, respectively. In (c), staining with an isotype control antibody is included as a control (grey dotted line). While histograms in c, e, g are representative images, the bar graphs in d, f, h summarise the MFI of (d) TAPBPR binding and (f and h) fluorescent peptide binding in the presence and absence of TAPBPR, from three independent experiment ± SD. ****p ≤ 0.0001, ***p ≤ 0.001, P** ≤ 0.01, *p ≤ 0.05.

https://doi.org/10.7554/eLife.40126.029

To explore whether the F pocket specificity of MHC I molecules was indeed crucial for allowing TAPBPR-mediated peptide exchange, we altered the F pocket of both HLA-A*68:02 and -A*68:01, by switching their residues on position 116, producing HLA-A*68:02Y116D and HLA-A*68:01D116Y. We subsequently tested their ability to bind soluble TAPBPR. When transduced into HeLaM-HLA-ABCKO cells, the 116-mutated HLA-A68 molecules were expressed at equivalent levels as their WT counterparts (Figure 7b). When we tested the ability of soluble TAPBPRWT to bind to cells expressing the HLA-A*68:02 molecules, strikingly, TAPBPR exhibited extremely low levels of binding to HLA-A*68:02Y116D compared to HLA-A*68:02WT molecules (Figure 7c and d). Conversely, TAPBPR binding to HLA-A*68:01 was significantly enhanced upon mutation of residue 116 from D to Y (Figure 7c and d). The influence of altering the F pocket on TAPBPR binding was further verified on another MHC I molecule, HLA-A2 (Figure 7—figure supplement 1). Together, these results demonstrate that the binding of TAPBPR to MHC I molecules is directly influenced by the architecture of the F pocket.

Mutation of the MHC I F pocket alters TAPBPR-mediated peptide editing

Next, we tested the ability of TAPBPRWT to mediate peptide exchange on the same panel of HLA-A68 molecules. Since HLA-A*68:01 and -A*68:02 have similar residues on position 114, we hypothesized that swapping their 116 residues might also swap their F pocket specificities. Thus, we interrogated the ability of these molecules to bind both the HLA-A*68:02-specific peptide ETVSK*QSNV, as well as the HLA-A*68:01-specific peptide KTGGPIYK*R, in the presence or absence of soluble TAPBPR. While TAPBPRWT efficiently mediated the loading of ETVSK*QSNV onto HLA-A*68:02WT molecules, it failed to load this peptide onto HLA-A*68:02Y116D molecules (Figure 7e and f). Similarly, TAPBPRWT failed to load ETVSK*QSNV onto HLA-A*68:01WT molecules, however, efficiently loaded this peptide onto HLA-A*68:01D116Y (Figure 7e and f). Moreover, we found that while TAPBPRWT could not load KTGGPIYK*R onto HLA-A*68:02WT molecules, it could efficiently load this peptide onto HLA-A*68:02Y116D (Figure 7g and h). Likewise, while HLA-A*68:01WT could bind KTGGPIYK*R in the presence of TAPBPRWT, no loading of this peptide was observed onto HLA-A*68:01D116Y (Figure 7g and h). These results are in keeping with the fact that the alterations made to the F pocket of the HLA-A68 molecules, and more specifically, to residue 116 alone, have altered peptide specificity. Nonetheless, it is interesting that although low levels of TAPBPR binding are observed to both HLA-A*68:02Y116D and HLA-A*68:01WT, TAPBPR is still capable of mediating peptide exchange on these molecule (albeit to a significantly lower extent as compared to their Y116-containing counterparts) and of loading the correct peptide based on their F pocket anchor.

Discussion

Given the discordance regarding the proximity of the TAPBPR loop in relation to the MHC I peptide binding groove in the recently captured structures (Jiang et al., 2017; Thomas and Tampé, 2017), and the lack of functional evidence to support any role for this loop, it was vital to directly determine whether this loop contributed at all to peptide exchange. Here, we reveal that upon mutation of the K22-D35 loop, TAPBPR retains binding to MHC I but loses its ability to effectively mediate peptide dissociation. Thus, our work provides compelling evidence that this loop region is critical for TAPBPR to mediate efficient peptide exchange and consequently peptide selection on MHC I molecules. We establish that the leucine residue in the loop is both necessary and sufficient for TAPBPR to promote peptide dissociation from both human and mouse MHC I molecules, which all typically accommodate a hydrophobic amino acid in their F pocket. Moreover, our findings also demonstrate that the ability of TAPBPR to exchange peptides is severely impaired on MHC I molecules whose F pockets accommodate charged residues. Furthermore, TAPBPR-mediated peptide editing in such scenarios occurs in a loop-independent manner. The differences identified here in the loop-dependency for TAPBPR-mediated editing amongst the various MHC I molecules are striking, especially when comparing HLA-A*68:02 with HLA-A*68:01, which only differ from each other by five amino acids, three of which are located around the F pocket. The extreme differences between these two HLA-A*68 molecules in regard to their susceptibility to TAPBPR-mediated peptide exchange has a remarkable resemblance to the findings reported for HLA-B44 molecules in regard to their dependency on tapasin for optimal peptide selection (Williams et al., 2002; Garstka et al., 2011). Particularly striking is that residue 116 of HLA I, which severely impacts both the specificity and architecture of the F pocket, seems to play a key role in allowing efficient binding of TAPBPR to HLA molecules.

Although mutation of the loop severely impairs the capacity of TAPBPR to dissociate peptides from MHC I, it seems that TAPBPR is still able, though to a considerably lower extent, to perform this function independently of the loop, across MHC I molecules with different binding motifs. This suggests that there are additional mechanisms by which TAPBPR is capable of mediating peptide exchange on MHC I. These may involve other regions of TAPBPR (for example the jack hairpin [Thomas and Tampé, 2017]) and/or could take place according to the recently proposed model of negative allosteric release (McShan et al., 2018). Chaperone-mediated peptide editing in such loop-independent scenarios may occur in a more generic and less efficient manner as compared to loop-dependent situations, and may be influenced mainly by the relative stability of individual peptide:MHC I complexes. Thus, one can envisage that TAPBPR can associate with MHC I molecules containing peptides that are intrinsically prone to dissociation (i.e. sub-optimally loaded peptide) in a loop-independent manner.

The functional evidence provided here highlights that the process of peptide editing is complex, multifaceted and is likely to involve dynamic movements of the loop region of TAPBPR as it probes the contents of the MHC I peptide binding groove. Our findings therefore help to explain the ambiguities in the loop conformation identified between the two solved crystal structures. Indeed, our data regarding the critical role of different residues in the MHC I groove to alter peptide exchange efficiency by TAPBPR may implicate an intrinsic difference in the ability of the specific mouse MHC I molecules crystallised with TAPBPR to accommodate hydrophobic residues in their F pocket. This may be of particular importance in the structure where modifications were made to H-2Dd to help stabilise it in a peptide-receptive form (Jiang et al., 2017), which may have occluded the ability of the loop to insert into the F pocket.

Our findings are supportive of the following molecular mechanism for peptide exchange by TAPBPR (Figure 8). When MHC I enters a state in which the bound peptide partially dissociates (i.e. 'breathes') at the C-terminus from the F pocket, TAPBPR binds to the MHC I groove, at this point in a transient manner, inserting its 22–35 loop into the groove. The leucine 30 residue of the TAPBPR loop binds into the F pocket of the groove, inhibiting the reassociation of the C-terminal anchor residue of the peptide, if hydrophobic, in a competitive manner. The resulting high-free energy intermediate allows TAPBPR to force the MHC I into a conformation to which it can bind more stably, pulling the α2–1 region of the peptide binding groove away from the peptide, as captured in the crystal structures (Jiang et al., 2017; Thomas and Tampé, 2017). This further prevents the rebinding of the peptide to the groove and thus promotes complete peptide dissociation. Given its stable interaction with peptide-receptive MHC I molecules, TAPBPR prevents these empty MHC I molecules from ‘crashing’ and we speculate that this consequently allows binding of incoming peptides with affinities for MHC I above the threshold required to outcompete TAPBPR. This final step may share similar features with the tug-of-war model that was previously proposed for tapasin (Fisette et al., 2016).

Figure 8
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Proposed model of the peptide exchange mechanism of TAPBPR on MHC class I.
https://doi.org/10.7554/eLife.40126.031

Intriguingly, our data suggests that the loop enables TAPBPR to dissociate peptides with a relatively high affinity for MHC I. This ability of TAPBPR to use its loop to lever peptide out of the MHC I binding groove is consistent with TAPBPR performing peptide editing after tapasin. The identification here of the critical importance of the leucine residue in the TAPBPR loop raises interesting questions regarding the different properties of the TAPBPR and tapasin loops and how these mediate step-wise editing to ensure optimal peptide loading of MHC I.

We have recently shown that both plasma membrane-targeted TAPBPR and exogenous soluble TAPBPR can be used to display immunogenic peptides on cell surface MHC I molecules, and, consequently, induce T-cell-mediated killing of target cells (Ilca et al., 2018). This observation presents previously unappreciated translational opportunities for utilising TAPBPR as a future immunotherapeutic. For example, TAPBPR could potentially be used to load immunogenic peptides onto tumours to target them from recognition by cytotoxic T cells. Utilising TAPBPR in this manner may promote tumour immunogenicity and increase the clinical efficacy of immune checkpoint inhibitors when used in combination. Here, we have demonstrated the essential role of the TAPBPR loop in the loading of exogenous peptides onto cell surface MHC I molecules and highlight the critical importance of incorporating this structural motif of TAPBPR into compounds developed with a therapeutic intent. Thus, innovative design of the loop may help tailor TAPBPR to specific MHC I molecules and/or alter the properties of the immunogenic peptides loaded by TAPBPR. For example, insight regarding the loop may allow us to tailor TAPBPR to MHC I molecules on which the naturally occurring loop does not work. Furthermore, we may also be able to improve peptide binding on MHC I molecules on which TAPBPR is already able to work on. Consequently, insight into the functional residues of the loop could expand the range of patients that may be responsive to TAPBPR-based therapies.

Materials and methods

Docking of TAPBPR with MHC I

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Dockings of TAPBPR with MHC I were carried out prior to the recent structure determination of the TAPBPR:MHC I complex structures. For the docking studies, we used our previously determined model for human TAPBPR based on the structure of tapasin (Hermann et al., 2013) and human HLA-A2 (Saper et al., 1991) (PDB ID 3HLA). TAPBPR was manually docked onto MHC I with the following restraints: (i) the membrane-proximal domains of each component, α3 of MHC I and the IgC domain of TAPBPR were oriented so as to allow these proteins to maintain complex formation when membrane-embedded; (ii) the surface shape complementarity of the components was maintained; (iii) the side chain of residue T134 of MHC I was kept within 10 Å of the sidechains of residues E205-Q209 (TN6 patch) and I261 (TN5 patch) on the IgV domain of TAPBPR; and (iv) sidechains of E222 and D223 of MHC I were kept within 10 Å of the sidechains of residues R335 (TC2) and Q336-S337 (TC3) on the IgC domain of TAPBPR. These last two restraints being mutations known to knock out the interaction in cells (Hermann et al., 2013) and the relatively loose restraints allowing for some conformational flexibility of the components upon complex formation.

Constructs and cell lines

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HeLaM cells, a variant HeLa cell line that is more responsive to IFN (Tiwari et al., 1987) (a gift from Paul Lehner, University of Cambridge, UK), their modified variants, and HEK-293T (from Paul Lehner, University of Cambridge, UK) were maintained in Dulbecco's Modified Eagle's medium (DMEM; Sigma-Aldrich, UK), supplemented with 10% fetal calf serum (Gibco, Thermo Fisher Scientific), 100 U/ml penicillin and 100 µg/ml streptomycin (Gibco, Thermo Fisher Scientific) at 37°C with 5% CO2. All cells were confirmed to be mycoplasma negative (MycoAlert, Lonza, UK). Authenticity of HeLaM was verified by tissue typing for HLA molecules and by the continuous confirmation that these cell lines had the expected HLA class I tissue type monitored by staining with specific HLA antibodies and by mass spectrometry.

TAPBPRWT and TAPBPRTN5 constructs cloned in the lentiviral vector pHRSIN-C56W-UbEM have previously been described (Boyle et al., 2013; Hermann et al., 2013). TAPBPRØloop, in which amino acids comprising the 22–35 loop were replaced with amino acids glycine, alanine and serine, was created from TAPBPRWTpHRSIN-C56W-UbEM using the following procedure: First, amino acids 22–28 were replaced using quick-change site-directed mutagenesis using primers M22-for and M22-rev (see Table 2 for primer sequences). Subsequently, amino acids 29–35 were replaced using a two-step PCR. For this, the TAPBPR insert was amplified in two separate pieces, starting from each side of the mutation site (primers TAPBPRWT-BamHI-for and M29-rev for the N terminus-containing side and primers M29-for and TAPBPRWT-NotI-rev for the C terminus-containing side). Subsequently, the two pieces bearing complementary regions over the mutated site were used in a second PCR reaction to amplify the whole TAPBPR mutated insert using primers TAPBPRWT-BamHI-for and TAPBPRWT-NotI-rev. TAPBPRL30G and TAPBPRØG30L were generated from TAPBPRWT and TAPBPRØloop respectively, using primers L30G-for and L30G-rev or ØG30L-for and ØG30L-rev, by quick change site-directed mutagenesis.

Table 2
Primers used for cloning and generation of TAPBPR loop mutants.
https://doi.org/10.7554/eLife.40126.032
Primer namePrimer sequence 5ʹ−3ʹ
TAPBPRWT- BamHI-forGCGCGGATCCAGCAGCCTCCATGGGCACACAGGAGGGC
TAPBPRWT- NotI-revGCGCGCGGCCGCTCAGCTGGGCTGGCTTACA
M22-forGTCCTAGACTGTTTCCTGGTGGCGGCCGGTGGGAGCGGTGGAGCTCTCGCCAGCAGTG
M22-revCACTGCTGGCGAGAGCTCCACCGCTCCCACCGGCCGCCACCAGGAAACAGTCTAGGAC
M29-forGCGGCCGGTGGGAGCGGTGGAGGTGGCAGCGGCGGTG
M29-revTCCACCGCTCCCACCGGCCGCCACCAGGAAACAGTCTAGGAC
L30G-forGTGGAGCTGGCGCCAGCAGT
L30G-revACTGCTGGCGCCAGCTCCAC
ØG30L-forGGTGGAGGTCTGGGCGGCGGTGC
ØG30L-revGCACCGCCGCCCAGACCTCCACC

The HLA-A*68:02WT construct was cloned by consecutive rounds of quick-change site-directed mutagenesis, using the HLA-A*68:01WT construct as a template (see Table 3 for primer sequences). Since residue 116 was mutated last in this process, the HLA-A*68:02Y116D mutant was the final intermediate in this cloning process. The HLA-A*68:01D116Y was cloned by quick-change site-directed mutagenesis, using primers A6801-D116Yonly-Fwd and A6801-D116Yonly-Rev. The HLA-A2Y116D mutant was cloned by quick-change site-directed mutagenesis, using primers A2-Y116D-Fwd and A2-Y116D-Rev.

Table 3
Primers used for generating HLA mutations.
https://doi.org/10.7554/eLife.40126.033
Primer namePrimer sequence 5'−3'
A6801_V12M_FwdCTACACTTCCATGTCCCGGC
A6801_V12M_RevGCCGGGACATGGAAGTGTAG
A6801_M97R_FwdCACCATCCAGAGGATGTATGGC
A6801_M97R_RevGCCATACATCCTCTGGATGGTG
A6801_S105P_FwdCGTGGGGCCGGACGGGC
A6801_S105P_RevGCCCGTCCGGCCCCACG
A6801_R114H_FwdGCGGGTACCACCAGGACGCC
A6801_R114H_RevGGCGTCCTGGTGGTACCCGC
A6801_D116Y_FwdGTACCACCAGTACGCCTACG
A6801_D116Y_RevCGTAGGCGTACTGGTGGTAC
A6801_D116Yonly_FwdGTACCGGCAGTACGCCTAC
A6801_D116Yonly_RevGTAGGCGTACTGCCGGTAC
A2_Y116D_FwdGTACCACCAGGACGCCTACG
A2_Y116D_RevCGTAGGCGTCCTGGTGGTAC

Reconstitution of the TAPBPR variants into the TAPBPR-knockout HeLaM cell line (HeLaM-TAPBPRKO), and HLA into the HeLaM-HLA-ABCKO cells was performed using lentiviral transduction and the cells were subsequently cultured as previously described (Neerincx et al., 2017; Neerincx and Boyle, 2018). Cells were induced with 200 U/ml IFN-γ (Peprotech, UK) for 48–72 hr where indicated.

Expression and purification of TAPBPR protein

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To make secreted forms of the TAPBPR loop variants enumerated above, the lumenal domains were cloned into a modified version of the PB-T-PAF vector where the N-terminal Protein A fusion was removed and a C-terminal His6 tag introduced and expressed in 293 T cells using the PiggyBac expression system (Li et al., 2013). 48 hr after transfection, cells were transferred for at least 5 days into selection media (DMEM supplemented with 10% FBS, 1% pen/strep, 3 µg/mL puromycin (Invivogen, San Diego, CA) and 700 µg/mL geneticin (Thermo Fisher Scientific, UK). To induce protein expression, cells were harvested and transferred into DMEM supplemented with 5% FBS, 1% pen/strep and 2 µg/mL doxycycline (Sigma-Aldrich, UK). After 5–7 days, the media was collected and TAPBPR was purified using Ni-NTA affinity chromatography. For purity assessment, elution fractions were analysed by SDS-PAGE, followed by Coomassie staining

Differential scanning fluorimetry (DSF)

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Thermofluor experiments were performed in 96-well low profile clear PCR plates for Viia7 cyclers (Axygen). Reactions of 20 μl comprised of 5 μg protein, 1x protein Thermal Shift Dye (Life Technologies) in PBS pH 7.4. The melting curve was performed using a Viia7 thermocycler between 20°C and 95°C in 1°C steps with 20 s equilibration time per step and fluorescence monitored on the ROX channel.

Isolation of HLA peptides

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HLA class I molecules were isolated from HeLaM-TAPBPRKO cells transduced with either TAPBPRWT, TAPBPRØloop or TAPBPRØG30L using standard immunoaffinity chromatography employing the pan-HLA class I-specific antibody W6/32 (produced in-house), as described previously (Kowalewski and Stevanović, 2013). Tissue typing confirmed the HeLaM cells express HLA-A*68:02, -B*15:03 and –C*12:03.

Analysis of HLA ligands by LC-MS/MS

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Isolated HLA peptides were analysed in five technical replicates. Peptide samples were separated by nanoflow high-performance liquid chromatography (RSLCnano, Thermo Fisher Scientific, Waltham, MA) using a 50 μm × 25 cm PepMap rapid separation liquid chromatography column (Thermo Fisher Scientific) and a gradient ranging from 2.4% to 32.0% acetonitrile over the course of 90 min. Eluting peptides were analyzed in an online-coupled LTQ Orbitrap XL mass spectrometer (Thermo Fisher Scientific) using a top five CID (collision-induced dissociation) fragmentation method.

Database search and HLA annotation

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Spectra were annotated to corresponding peptide sequences by database search of the human proteome as comprised in the Swiss-Prot database (20,279 reviewed protein sequences, September 27th 2013) by employing the SEQUEST HT search engine (Eng et al., 1994) (University of Washington) integrated into ProteomeDiscoverer 1.4 (Thermo Fisher Scientific). Data processing was performed without enzyme specificity, with peptide length limited to 8–12 amino acids, and methionine oxidation set as dynamic modification. The false discovery rate was calculated by the Percolator algorithm (Käll et al., 2007) and set to 5%. HLA annotation was performed using NetMHCpan-4.0 with a percentile rank threshold of 2%.

Label-free quantitation

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We used label-free quantitation (LFQ) as described previously (Nelde et al., 2018) to assess the relative HLA ligand abundances between TAPBPRWT, TAPBPRØloop or TAPBPRØG30L expressing cells. Briefly, relative quantitation of HLA ligands was performed by calculating the area under the curve of the respective precursor extracted ion chromatogram (XIC) using ProteomeDiscoverer 1.4 (Thermo Fisher Scientific). For LFQ analysis the total injected peptide amount of all samples was normalised prior to LC-MS/MS analysis. Volcano plots were computed using an in-house R script (v3.2) and depict pairwise comparisons of the ratios of the mean areas for each individual peptide in the five LFQ-MS runs. Significant modulation was defined by an adjusted p-value of < 0.01 and a fold change of ≥ log2 2 fold change, as calculated by two-tailed t-tests implementing Benjamini-Hochberg correction.

MHC I-binding peptides

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The following fluorescent MHC I-specific peptides were used (K* represents a lysine labelled with 5-carboxytetramethylrhodaime [TAMRA]): ETVSK*QSNV (HLA-A*68:02), YVVPFVAK*V (HLA-A*68:02), NLVPK*VATV (HLA-A*02:01), YLLEK*LWRL (HLA-A*02:01), SIINFEK*L (H-2Kb), and KTGGPIYK*R (HLA-A*68:01). The following unlabeled competitor peptides were used: YVVPFVAKV, which exhibits high affinity of HLA-A*68:02 and EGVSEQSNG, a non-binding derived of ETVSEQSNV, obtained by replacing its anchor residues (amino acids on positions 2 and 9) with glycine. All peptides were purchased from Peptide Synthetics, UK.

Antibodies

TAPBPR was detected using either PeTe4, a mouse monoclonal antibody (mAb) specific for the native conformation of TAPBPR, raised against amino acids 22–406 of human TAPBPR (Boyle et al., 2013) that does not cross-react with tapasin (Hermann et al., 2013), or ab57411, a mouse mAb raised against amino acids 23–122 of TAPBPR that is reactive to denatured TAPBPR (Abcam, UK). UGT1 was detected using the rabbit mAb ab124879 (Abcam). MHC class I heavy chains were detected using mAb HC10 (Stam et al., 1986). Soluble TAPBPR variants were detected using the mouse anti-polyhistidine mAb H1029 (Sigma-Aldrich). A mouse IgG2a isotype control was also used as a control (Sigma-Aldrich).

Flow cytometry

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Following trypsinisation, cells were washed in 1% bovine serum albumin (BSA), dissolved in 1x phosphate-buffered saline (PBS) at 4°C, and then stained for 30 min at 4°C in 1% BSA containing with PeTe4 or with an isotype control antibody. After washing the cells to remove excess unbound antibody, the primary antibodies bound to the cells were detected by incubation at 4°C for 25 min with either goat anti-mouse Alexa-Fluor 647 IgG (Invitrogen Molecular Probes, Thermo Fisher Scientific). After subsequent three rounds of washing, the fluorescence levels were detected using a BD FACScan analyser with Cytek modifications and analysed using FlowJo (FlowJo, LLC, Ashland, OR).

Peptide binding assay

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For peptide binding in the presence of recombinant TAPBPR, the cells were treated with or without the indicated concentration of recombinant TAPBPR (unless otherwise indicated, we used 100 nM for HLA-A*68:02, 1 µM used for HLA-A*02:01, H-2Kb and HLA-A*68:01). After 15 min, the desired TAMRA-labelled peptide was added to the cells and incubated at 37°C (15 min for HLA-A*68:02, 60 min for HLA-A*02:01 and –A*68:01, or 30 min for H-2Kb). For experiments performed at 26°C, following cell seeding and IFNγ stimulation at 37°C for 48 hr, cells were transferred at 26°C for another 12 hr to allow for the expression of sub-optimally loaded MHC I molecules at the cell surface. The TAPBPR and peptide binding was then performed as described above, with all incubation steps being performed at 26°C instead of 37°C (with the exception of cell trypinisation which was carried out at 37°C). In cases where the peptide binding was facilitated by over-expressed TAPBPR, the labelled peptide was directly added to the cells, without using recombinant TAPBPR. Following the peptide treatment, the cells were washed three times in 1x PBS and harvested. The level of bound peptide/cell was determined by flow cytometry, using the YelFL1 channel (Cytek).

Peptide exchange assay

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IFNγ-treated HeLaM-TAPBPRKO cell lines reconstituted with the different TAPBPR variants, were treated with 10 nM TAMRA-labelled peptide of interest diluted in opti-MEM for 15 min at 37°C, as described above. Following the binding step, the peptide-containing media was removed, the cells were washed and then treated with media alone or with different concentrations of non-labelled peptide for another 15 min at 37°C. The cells were then washed and harvested and the level of bound peptide per cell was determined by flow cytometry, using the YelFL1 channel (Cytek).

Immunoprecipitation, gel electrophoresis and western blotting

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For TAPBPR immunoprecipitation experiments from cells over-expressing the panel of TAPBPR variants, cells were lysed in 1% triton X-100 (VWR, Radnor, PN), Tris-buffered saline (TBS) (20 mM Tris-HCl, 150 mM NaCl, 2.5 mM CaCl2) supplemented with 10 mM N-ethylmaleimide (NEM), 1 mM phenylmethylsulfonyl fluoride (PMSF) (Sigma-Aldrich), and protease inhibitor cocktail (Roche, UK) for 30 min at 4°C. Nuclei and cell debris were pelleted by centrifugation at 13,000 × g for 15 min and supernatants were collected. Immunoprecipitation was performed with the TAPBPR-specific mAb PeTe4 (Boyle et al., 2013) coupled to protein A sepharose (GE Healthcare), at 5 µg antibody per sample, for 2 hr at 4°C with rotation. Following immunoprecipitation, beads were washed thoroughly in 0.1% detergent-TBS to remove.

For pulldown experiments using soluble TAPBPR proteins, IFNγ-stimulated HeLa-TAPBPRKO cells were harvested, lysed and cleared of cell debris as above. In order to remove cellular factors which bind non-specifically to the sepharose beads, the cell lysate was pre-cleared by treatment with protein A sepharose alone, for 30 min at 4°C. Subsequently, the lysate was aliquoted and incubated with 5 µg of the soluble TAPBPR variant for 90 min at 4°C. Immunoprecipitation of soluble TAPBPR was performed using PeTe4 as above. Soluble TAPBPR was detected on western blots with the anti-polyHis primary antibody. Gel electrophoresis and western blot analysis was performed as described in Neerincx et al. (2017).

Data availability

All data generated or analysed during this study are included in the manuscript and supporting files. Source data files regarding the lists of peptides presented on MHC class I have been provided for Figures 5.

The following data sets were generated
    1. Ilca FT
    2. Neerincx A
    3. Hermann C
    4. Marcu A
    5. Stevanovic S
    6. Deane JE
    7. Boyle L
    (2018) Dryad
    Data from: TAPBPR mediates peptide dissociation from MHC class I using a leucine lever.
    https://doi.org/10.5061/dryad.p5k0156

References

    1. Stam NJ
    2. Spits H
    3. Ploegh HL
    (1986)
    Monoclonal antibodies raised against denatured HLA-B locus heavy chains permit biochemical characterization of certain HLA-C locus products
    Journal of Immunology 137:2299–2306.

Decision letter

  1. Michel C Nussenzweig
    Senior Editor; The Rockefeller University, United States
  2. Kai Wucherpfennig
    Reviewing Editor; Dana-Farber Cancer Institute, Harvard Medical School, United States
  3. Nilabh Shastri
    Reviewer; University of California, Berkeley, United States
  4. Peter Van Endert
    Reviewer; Institut National de la Santé et de la Recherche Médicale, France

In the interests of transparency, eLife includes the editorial decision letter and accompanying author responses. A lightly edited version of the letter sent to the authors after peer review is shown, indicating the most substantive concerns; minor comments are not usually included.

Thank you for submitting your article "TAPBPR mediates peptide dissociation from MHC class I using a leucine lever" for consideration by eLife. Your article has been reviewed by three peer reviewers, one of whom is a member of our Board of Reviewing Editors, and the evaluation has been overseen by Michel Nussenzweig as the Senior Editor. The following individuals involved in review of your submission have agreed to reveal their identity: Nilabh Shastri (Reviewer #2); Peter Van Endert (Reviewer #3).

The reviewers have discussed the reviews with one another and the Reviewing Editor has drafted this decision to help you prepare a revised submission.

Summary:

This manuscript demonstrates the function of a TAPBPR loop in peptide editing of MHC class I proteins. In particular, the authors highlight a hydrophobic residue in this loop as being important in peptide exchange for MHC class I proteins with a hydrophobic F pocket. The data suggest a model in which the TAPBPR loop competes with the peptide side chain for access to the F pocket and thereby favors peptide exchange as well as editing.

Essential revisions:

1) Strengthening of immunopeptidomics data with biological replicates.

2) Examining why TAPBPR has a preference for A:68:02, but not the other studied class I proteins that also have a hydrophobic F pocket.

3) Studying whether TAPBPR indeed mediates peptide exchange at the cell surface in their assays or in an intracellular recycling compartment.

4) Proving that L30 of TAPBPR indeed binds in the F pocket.

5) Studying the biochemistry of MHC class I – TAPBPR interaction with purified soluble proteins.

Reviewer #1:

This is an interesting study that examines the functional significance of a loop of TAPBPR in the editing of MHC class I bound peptides. The authors show that this loop has a major effect on the efficiency of peptide editing. In particular, they implicate a hydrophobic residue (L30) in this loop. Functional data suggest that this hydrophobic residue binds in the F pocket of certain MHC class I proteins and thereby directly competes with the peptide C-terminus.

1) One of the major conclusions is that residue L30 of the TAPBPR loops binds in the F pocket of MHC class I proteins. While the data are consistent with this conclusion, it is important to directly prove this point. This could, for example, be done by working with a HLA-A*68:02 mutant in which only residues of the F pocket are changed.

2) In the mass spec experiment shown in Figure 2, the representation of HLA-A*68:02 peptides is greatly increased in the most severe loop mutant. The opposite result would be expected based on the other functional data. Is this because peptide binding to HLA-B*15:03 and/or HLA-C*12:03 is even more severely affected?

3) All experiments were performed with cell surfaced expressed MHC class I/peptide complexes. However, the major site of peptide editing by TAPBPR is thought to be the ER. It would therefore be useful to assess the effect of TAPBPR with soluble MHC class I/peptide complexes. Soluble TAPBPR is already available.

Reviewer #2:

In this manuscript, Ilca and colleagues provide insights into the molecular mechanism by which TAPBPR mediates exchange of peptides within the MHC I cargo. TAPBPR is a second peptide editor that functions independently of the peptide-loading complex in the endoplasmic reticulum. However the molecular mechanism by which TAPBPR causes exchange of the peptide bound to MHC I is unknown.

Based upon the crystal structure of TAPBPR (Thomas and Tampe, 2011), the authors here analyzed the potential functional significance of a 14 residue (aa 22-35) loop in this molecule. They first replaced all residues in this loop with either glycine, alanine or serine to generate a "functionless" loop (TAPBPRØloop). Additionally they generated two mutants one of WT TAPBPR with the Leu30Gly mutation and another of the TAPBPRØloop in which the Leu residue was reinserted in position 30 (TAPBPR0G30L). DNA constructs encoding each of these molecules when transfected in HeLa cells (lacking endogenous TAPBPR) were similarly expressed and co-immunoprecipitated with both MHC I and the UGT1.

Analysis of the MHC associated peptides by mass spectrometry showed large changes in the peptide repertoire generated in cells expressing WT TAPBPR versus the Øloop mutant as well as the 0G30L mutant versus WT TAPBPR. Interestingly, the changes in the peptide repertoire were particularly marked for HLA*A68 relative to other HLA allotypes. This is an interesting result that suggests that TAPBPR could have selective effects on some but not other MHC I isotypes.

Further, using novel peptide exchange assays developed earlier by this group, the authors showed that the absence of the loop residues (TAPBPRØloop) rendered the molecule non-functional as did the replacement L30G in WT TAPBPR. This loss of peptide exchange activity was substantially although not completely restored by substitution G30L in the TAPBPRØloop mutant.

Altogether, the authors present a nice story with compelling data to support their conclusions. Their model to explaining how TAPBPR causes weakly bound peptides to be dissociated from the MHC I molecule also looks reasonable. However, how the incoming peptide would get into the MHC I groove remains unexplained.

The paper is well written and most of the ideas come through clearly. I would however, have liked the authors to elaborate some more on the potential for therapeutic uses of their findings. As such the ending of the Discussion is rather vague.

The specific sequences identified by mass spectrometry and summarized in Figure 2 should be deposited in a publicly available database.

Reviewer #3:

Ilca and colleagues study the role of a loop extending from the TAPBPR peptide editor into the F pocket of MHC class I molecules. They present clear evidence for a critical and specific role of a Leu in the loop for editing of peptides with hydrophobic C-terminal residues. Globally the data is convincing and represents a significant progress in our understanding of peptide editing by TAPBPR.

However, I am not convinced with respect to the broad validity of the conclusions. Moreover, some problematic technical issues and data interpretations should be addressed.

- The immunopeptidomics data are apparently based on a single experiment with 5 technical replicates. Examining Figure 5—figure supplement 1, only about 57% of peptides are found in 4/5 or 5/5 replicates for WT, and about 38% for G30L. Considering this level of reproducibility for technical replicates, biological replicates can be expected to be even less reproducible. Relatively low reproducibility (which I believe is not unusual for MS data) also raises doubt about the data on relative peptide amounts. Showing at least one example of reproducibility for biological replicates would make the immunopeptidomics data more convincing.

- Together with data shown later in the paper, the peptidomics data are interpreted to indicate a specific effect of TAPBPR on peptides with hydrophobic C-terminals. However, checking the peptide binding motifs of B*15:03 and C*12:03, it turns out that both also bind peptides with hydrophobic C-terminals: Tyr/Phe for B*15:03, Leu/Met/Val/Phe for C*12:03. Is there any evidence, for example from modeling, that TAPBPR acts preferentially on aliphatic not aromatic hydrophobic C-terminals, explaining the opposite effect on B*15:03 (which would exclude many peptides from editing given the high frequency of class I ligands with Tyr/Phe)? Concerning the opposite effect on C*12:03 versus A*68:02, unless the authors have a good explanation for it, I would expect a cautious remark in the Discussion mentioning that the rules remain to be understood and might include other parameters than hydrophobicity.

- The fact that A*68:02, later shown to be particularly susceptible to editing by TAPBPR, acquires many more ligands in its absence could be seen as a bit counter-intuitive – why does A*68:02 present many peptides in larger amounts in the absence of TAPBPR? One explanation would be that these peptides have low affinity and would therefore be chased in its presence. This should be easy enough to check using the NetMHC algorithm used by the authors.

- Although the authors might show that in another paper (PNAS in revision?), the assumption that over-expressed membrane bound or exogenously added soluble TAPBPR acts directly on the plasma membrane would require some evidence. Class I molecules are known to recycle in HeLa cells and TAPBPR might well recycle with them, mediating intracellular peptide exchange. Incubations are performed for 15min at 37°C, sufficient time for recycling. Does soluble TAPBPR have an effect at low temperature?

- The authors conclude that the mutants bind to empty class I, based on an experiment in which they add TAPBPR to Triton lysates of cells. I feel that this conclusion would be more convincing if they used TAP ko cells for this demonstration. TAP ko cells express a majority of poorly loaded or "empty" class I molecules at the surface at 26°C. If the TAPBPR mutants indeed bind only to empty class I, then pre-incubating TAP ko cells at 26°C in the presence of high affinity peptides should abolish their interaction with class I.

https://doi.org/10.7554/eLife.40126.038

Author response

Essential revisions:

1) Strengthening of immunopeptidomics data with biological replicates.

We have included data on two additional biological replicates for TAPBPRWT TAPBPRØloop and TAPBPRØloopG30L. Furthermore, we have provided data for an additional TAPBPR loop mutant in which residues A29-D35 were mutant. The new data provided confirm that there is a significant change in peptide repertoire upon mutation of the loop.

2) Examining why TAPBPR has a preference for A:68:02, but not the other studied class I proteins that also have a hydrophobic F pocket.

We have provided additional data (see Figure 5—source data 1) that demonstrates that TAPBPR binds very strongly to HLA-A*68:02 but does not bind to any significant level to the two other HLA molecules, HLA-B*15:03 and Cw12, expressed in HeLa cells. Therefore, the apparent preference of TAPBPR to influence the peptide repertoire on HLA-A*68:02, and not the two other HLA molecules expressed in HeLa B*15:03 and C*12:03, seems to be related to the intrinsic ability of TAPBPR to bind to HLA molecule, in addition to the specificity of the F pocket for hydrophobic residues

3) Studying whether TAPBPR indeed mediates peptide exchange at the cell surface in their assays or in an intracellular recycling compartment.

We thank you for raising this important point and apologies for not making this obvious in our first draft of the manuscript. We have already extensively investigated this in our now recently published PNAS paper which demonstrates that surprising TAPBPR can mediate peptide even on cells incubated at 4oC, which inhibits intracellular recycling. This demonstrates that TAPBPR-mediated peptide exchange occurs directly on the cell surface. Now that the manuscript demonstrating this is published, we have highlighted this key point in the text in this manuscript. We also include similar data at 4oC for both TAPBPR-WT and the loop mutants in this manuscript, which can be found in Figure 2—figure supplement 1.

4) Proving that L30 of TAPBPR indeed binds in the F pocket.

We have now investigated this extensively and the data can be found in a new figure (new Figure 7). To test this, we introduced specific mutations to the F pocket of both HLA-A*68:02 and HLA-A*68:01. The data demonstrate that the residue found at position 116 of HLA I molecules is critical for allowing TAPBPR binding. Furthermore, TAPBPR selects the correct peptide corresponding to the F pocket specificity of the MHC I.

5) Studying the biochemistry of MHC class I – TAPBPR interaction with purified soluble proteins.

We have included the ability of soluble WT-TAPBPR and one loop mutant to bind to soluble variants of the three HLA molecules expressed in HeLa (HLA-A*68:02, -B15:03 and –Cw12:03). This reveals that TAPBPR binds extremely well to HLA-A*68:02, but does not bind to HLA-B*15:03 or Cw12:03. The provided data also shows that mutation of the loop severely impairs TAPBPR binding to soluble HLA-A*68:02. This new data can be found in Figure 5—source data 1.

Reviewer #1:

This is an interesting study that examines the functional significance of a loop of TAPBPR in the editing of MHC class I bound peptides. The authors show that this loop has a major effect on the efficiency of peptide editing. In particular, they implicate a hydrophobic residue (L30) in this loop. Functional data suggest that this hydrophobic residue binds in the F pocket of certain MHC class I proteins and thereby directly competes with the peptide C-terminus.

1) One of the major conclusions is that residue L30 of the TAPBPR loops binds in the F pocket of MHC class I proteins. While the data are consistent with this conclusion, it is important to directly prove this point. This could, for example, be done by working with a HLA-A*68:02 mutant in which only residues of the F pocket are changed.

Many thanks for suggesting this experiment. To explore this, we have compared the ability of TAPBPR to bind to both WT HLA-A*68:02 and HLA-A*68:01 and to their F pocket mutant counterparts (created by altering residue 116). The mutation of residue 116 changed F pocket specificity of both these MHC I molecules and also significantly altered the ability of TAPBPR to bind to them. More specifically, replacing residue 116 in A*68:02 with the one of A*68:01 reduced the ability of TAPBPR to bind and correspondingly, replacing residue 116 in A*68:01 with the one of A*68:02 increased the ability of TAPBPR to bind. This new data can be found in the new Figure 7. We then confirmed the importance of this residue for TAPBPR binding on a different MHC I molecule, HLA-A2. This can be found in figure 7—figure supplement 1. This new data helps highlight the critical importance of the F pocket in TAPBPR binding and peptide exchange.

2) In the mass spec experiment shown in Figure 2, the representation of HLA-A*68:02 peptides is greatly increased in the most severe loop mutant. The opposite result would be expected based on the other functional data. Is this because peptide binding to HLA-B*15:03 and/or HLA-C*12:03 is even more severely affected?

We have now provided evidence that TAPBPR binds extremely well to HLA-A*68:02, but not to HLA-B*15:03 and C*12:03. Thus, the increase in HLA-A*68:02 assignable peptide upon mutation of the loop is unlikely to be due to severe effect on HLA-B15:03 and C*12:03. Upon reflection of both yours and reviewer 3’s comment that the increase representation of HLA-A*68:02 is counter intuitive, we examined precisely how the cell were prepared for immunopeptidomic analysis. For the original dataset, we allowed the HeLaM cells to recover from trypsination by incubating them at 37oC in media, before freezing them for shipping for Immunopeptidomic analysis. Given that we have established that the small pool of surface TAPBPR is functional, we wondered whether the observed increase in HLA-A*68:02 assignable peptides was actually due to TAPBPR with a functional loop (i.e. TAPBPRWT and TAPBPRØloopG30L) stripping HLA-A*68:02 peptides from surface expressed MHC I. To explore this, we performed immunopeptidomic analysis on cells immediately after trypsination without the 37oC recovery step. While this additional dataset confirmed that mutation of the loop has a significant effect on the peptide repertoire presented, the peptides assignable to HLA-A*68:02 for TAPBPRWT and TAPBPRØloopG30L were now normalised. Thus, the observed increase in peptides assignable to HLA-A*68:02 for TAPBPRØloop expressing cells is not due a specific influence of this mutation on MHC I. Rather, in the original dataset (and in an additional biological repeat provided) the observed effect is due to TAPBPR with a functional loop stripping peptides from HLA-A*68:02 molecules. The loss in the ability of TAPBPR to dissociate peptides upon mutation of the loop explains the increased number of peptides assigned to HLA-A*68:02 for the Øloop mutant, as compared to both WT TAPBPR and the ØG30L mutant.

Note: We have rearranged the figure now in our manuscript and the immunopeptidomic data have moved further down the paper and can now be found in Figure 5.

3) All experiments were performed with cell surfaced expressed MHC class I/peptide complexes. However, the major site of peptide editing by TAPBPR is thought to be the ER. It would therefore be useful to assess the effect of TAPBPR with soluble MHC class I/peptide complexes. Soluble TAPBPR is already available.

In an attempt to address this point in the allocated time frame, we have compared the ability of both TAPBPRWT and TAPBPR lacking a function loop to bind to soluble peptide-bound HLA-A*68:02, B*15:03 and C*12:03, coupled to beads. This revealed TAPBPRWT binds strongly to HLA-A*68:02, but not to HLA-B*15:03 or C*12:03. Furthermore, this analysis reveals the decreased ability of TAPBPR to bind to HLA-A*68:02 upon mutation of the loop, which further confirms our other findings in the manuscript.

We agree that our assays test the ability of TAPBPR to mediate peptide exchange in an atypical location. However, the advantage of our systems, rather than using soluble MHC I, which has been refolded in bacteria, is that the MHC I (and also TAPBPR in one of our two assays) is membrane bound and also glycosylated, which reflects the MHC class I found in the ER more closely than using soluble MHC I. While our systems are not perfect and may not truly reflect what occurs in the ER, we feel these assays are superior to using soluble MHC I expressed in bacteria and refolded with a single peptide.

The immunopeptidomic analysis included reflects the effect of mutating the TAPBPR loop more widely on the whole cellular pool of MHC I. 95% of the TAPBPR:MHC I interactions in these experiments occurs in the ER.

Reviewer #2:

[…] All together, the authors present a nice story with compelling data to support their conclusions. Their model to explaining how TAPBPR causes weakly bound peptides to be dissociated from the MHC I molecule also looks reasonable. However, how the incoming peptide would get into the MHC I groove remains unexplained.

The paper is well written and most of the ideas come through clearly. I would however, have liked the authors to elaborate some more on the potential for therapeutic uses of their findings. As such the ending of the Discussion is rather vague.

The specific sequences identified by mass spectrometry and summarized in Figure 2 should be deposited in a publicly available database.

Many thanks for your positive review of our manuscript. In light of your comments we have included some additional text in the Discussion regarding how we envisage incoming peptide may get into the MHC I groove. Furthermore, we have attempted to strengthen the Discussion section regarding how knowledge of the functional importance of the loop prove insightful for therapeutic use. Finally, the specific sequence of the peptides identified by mass spectrometry have been deposited in a publicly available database.

Reviewer #3:

Ilca and colleagues study the role of a loop extending from the TAPBPR peptide editor into the F pocket of MHC class I molecules. They present clear evidence for a critical and specific role of a Leu in the loop for editing of peptides with hydrophobic C-terminal residues. Globally the data is convincing and represents a significant progress in our understanding of peptide editing by TAPBPR.

However, I am not convinced with respect to the broad validity of the conclusions. Moreover, some problematic technical issues and data interpretations should be addressed.

- The immunopeptidomics data are apparently based on a single experiment with 5 technical replicates. Examining Figure 5—figure supplement 1, only about 57% of peptides are found in 4/5 or 5/5 replicates for WT, and about 38% for G30L. Considering this level of reproducibility for technical replicates, biological replicates can be expected to be even less reproducible. Relatively low reproducibility (which I believe is not unusual for MS data) also raises doubt about the data on relative peptide amounts. Showing at least one example of reproducibility for biological replicates would make the immunopeptidomics data more convincing.

We have included data on two additional biological replicates for TAPBPRWT, TAPBPRØloop and TAPBPRØloopG30L. Furthermore, we have provided data for an additional TAPBPR loop mutant in which residues A29-D35 were mutant. The new data provided confirm that there is a significant change in peptide repertoire upon mutation of the loop.

- Together with data shown later in the paper, the peptidomics data are interpreted to indicate a specific effect of TAPBPR on peptides with hydrophobic C-terminals. However, checking the peptide binding motifs of B*15:03 and C*12:03, it turns out that both also bind peptides with hydrophobic C-terminals: Tyr/Phe for B*15:03, Leu/Met/Val/Phe for C*12:03. Is there any evidence, for example from modeling, that TAPBPR acts preferentially on aliphatic not aromatic hydrophobic C-terminals, explaining the opposite effect on B*15:03 (which would exclude many peptides from editing given the high frequency of class I ligands with Tyr/Phe)? Concerning the opposite effect on C*12:03 versus A*68:02, unless the authors have a good explanation for it, I would expect a cautious remark in the Discussion mentioning that the rules remain to be understood and might include other parameters than hydrophobicity.

Note: we have now rearranged the figures and the immunopeptidomic data can be found in Figure 5. We have included data on the ability of TAPBPR to bind to the individual HLA molecules found in HeLa (Figure 5—source data 1). This reveals TAPBPR binds well to HLA-A*68:02 but does not bind to HLA-B*15:03 or C*12:03. Thus, the reason we observe significant effects on HLA-A*68:02 but not the other HLA in HeLa can be explained by the ability of TAPBPR to bind to these individual HLA I molecules. In other words, we claim that while the F pocket specificity for hydrophobic residues is essential for TAPBPR function on MHC I molecules, not all MHC I molecules carrying a hydrophobic F pocket will interact well with TAPBPR.

- The fact that A*68:02, later shown to be particularly susceptible to editing by TAPBPR, acquires many more ligands in its absence could be seen as a bit counter-intuitive – why does A*68:02 present many peptides in larger amounts in the absence of TAPBPR? One explanation would be that these peptides have low affinity and would therefore be chased in its presence. This should be easy enough to check using the NetMHC algorithm used by the authors.

Upon reflection of your comment, we agree that this data is counter-intuitive.

Thus, as mention in our response to reviewer 1, we examined whether incubation of the cells for 30 min at 37oC in media after trypsination to allow them to recover was influencing the results for HLA-A*68:02, permitting functional TAPBPR (i.e. TAPBPRWT and TAPBPRØloopG30L) to strip peptides specifically from surface expressed HLA-A*68:02. When immunopeptidomic analysis was performed on cells immediately after trypsination without the 37oC recovery step, the peptides assignable to HLA-A*68:02 for TAPBPRWT and TAPBPRØloopG30L were now normalised. This data can be found in Figure 5. Thus, the loss in the ability of TAPBPR to dissociate peptides upon mutation of the loop explains the increased number of peptides assigned to HLA-A*68:02 for the Øloop mutant, as compared to both WT TAPBPR and the ØG30L mutant.

As suggested, we have performed NetMHC analysis on the peptides. This analysis can be found in Figure 5—figure supplement 4. However, we failed to see any reliable change in affinity of peptides. This is most likely due to limitations in our experimental design which was set up to explore whether there was global effects on mutation of the loop on MHC I peptide presentation, rather than to explore peptide affinity. Thus, in our experimental set up we need to assign peptides to one of the three MHC class I molecules found in HeLaM cells, which requires applying thresholds and cut-offs which will exclude low affinity peptides. In the future the effects of mutating the TAPBPR loop on MHC I peptide affinity could be explored by using cells expressing a single MHC I molecule.

- Although the authors might show that in another paper (PNAS in revision?), the assumption that over-expressed membrane bound or exogenously added soluble TAPBPR acts directly on the plasma membrane would require some evidence. Class I molecules are known to recycle in HeLa cells and TAPBPR might well recycle with them, mediating intracellular peptide exchange. Incubations are performed for 15min at 37°C, sufficient time for recycling. Does soluble TAPBPR have an effect at low temperature?

We have previously explored this in our manuscript recently published in PNAS, which unfortunately was not available to you at the time of your review of this manuscript. We have found that TAPBPR can indeed mediate peptide exchange on cells incubated at 4oC, which inhibits membrane trafficking.

We have included some text in the Results to highlight that we have previously shown that TAPBPR edits peptides on cell surface MHC I molecules. Furthermore, we have also included some data to show that we have performed similar experiments at 4oC for both TAPBPR-WT as well as the loop mutants. This can be found in the new Figure 2—figure supplement 1.

- The authors conclude that the mutants bind to empty class I, based on an experiment in which they add TAPBPR to Triton lysates of cells. I feel that this conclusion would be more convincing if they used TAP ko cells for this demonstration. TAP ko cells express a majority of poorly loaded or "empty" class I molecules at the surface at 26°C. If the TAPBPR mutants indeed bind only to empty class I, then pre-incubating TAP ko cells at 26°C in the presence of high affinity peptides should abolish their interaction with class I.

We have now included the variant of requested experiment which we believe helps to address the point you have raised here. We did not use T2 cells, instead we used the HeLaM-HLA-ABCKO overexpressing HLA-A*68:02 incubated -/+ high affinity peptide prior to incubation with soluble TAPBPR. Indeed, this revealed that pre-incubation with a high affinity peptide blocked the ability of TAPBPRØLoop to bind to the cells, but had no significant effect on the ability of TAPBPRWT to bind to cells. This new data can be found in Figure 4—figure supplement 1.

https://doi.org/10.7554/eLife.40126.039

Article and author information

Author details

  1. F Tudor Ilca

    Department of Pathology, University of Cambridge, Cambridge, United Kingdom
    Contribution
    Conceptualization, Data curation, Formal analysis, Writing—original draft, Writing—review and editing
    Competing interests
    Some aspects of the work included in this manuscript form part of a recent patent application. Applicant: Cambridge Enterprise Limited. Application number: 1801323.5, Status: Pending
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-6582-8007

  2. Andreas Neerincx

    Department of Pathology, University of Cambridge, Cambridge, United Kingdom
    Contribution
    Conceptualization, Resources, Data curation, Supervision
    Competing interests
    Some aspects of the work included in this manuscript form part of a recent patent application. Applicant: Cambridge Enterprise Limited. Application number: 1801323.5, Status: Pending
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-6902-5383

  3. Clemens Hermann

    Department of Integrative Biomedical Sciences, Division of Chemical and Systems Biology, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa
    Contribution
    Conceptualization, Formal analysis
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-0009-9501

  4. Ana Marcu

    Department of Immunology, Interfaculty Institute for Cell Biology, University of Tübingen, Tübingen, Germany
    Contribution
    Data curation, Formal analysis, Writing—review and editing
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-0808-8097

  5. Stefan Stevanović

    1. Department of Immunology, Interfaculty Institute for Cell Biology, University of Tübingen, Tübingen, Germany
    2. DKFZ Partner Site Tübingen, German Cancer Consortium, Tübingen, Germany
    Contribution
    Supervision, Funding acquisition, Methodology
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-1954-7762

  6. Janet E Deane

    Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom
    Contribution
    Conceptualization, Supervision, Methodology, Writing—original draft, Writing—review and editing
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-4863-0330

  7. Louise H Boyle

    Department of Pathology, University of Cambridge, Cambridge, United Kingdom
    Contribution
    Conceptualization, Resources, Formal analysis, Supervision, Funding acquisition, Writing—original draft, Project administration, Writing—review and editing
    For correspondence
    lhb22@cam.ac.uk
    Competing interests
    Some aspects of the work included in this manuscript form part of a recent patent application. Applicant: Cambridge Enterprise Limited. Application number: 1801323.5, Status: Pending
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-3105-6555

Funding

Wellcome Trust (104647/Z/14/Z)

  • Andreas Neerincx
  • Louise H Boyle

South African Medical Research Council

  • Clemens Hermann

Royal Society (UF100371)

  • Janet E Deane

Bosch-Forschungsstiftung

  • Ana Marcu
  • Stefan Stefvanovic

Wellcome Trust (109076/Z/15/A)

  • Florin Tudor Ilca

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Acknowledgements

We are extremely grateful to Gemma Brewin and Sarah Peacock (Tissue Typing Laboratory, Cambridge University Hospitals NHS Foundation Trust) for both the use of and their guidance using the LABScreen single antigen HLA class I beads and the Luminex Fluoroanalyser system. We thank Ben Challis and John Trowsdale from the University of Cambridge for proofreading our manuscript and Nico Trautwein from Eberhard Karls University Tübingen for assistance with Immunopeptidomics.

Senior Editor

  1. Michel C Nussenzweig, The Rockefeller University, United States

Reviewing Editor

  1. Kai Wucherpfennig, Dana-Farber Cancer Institute, Harvard Medical School, United States

Reviewers

  1. Nilabh Shastri, University of California, Berkeley, United States
  2. Peter Van Endert, Institut National de la Santé et de la Recherche Médicale, France

Publication history

  1. Received: July 15, 2018
  2. Accepted: November 28, 2018
  3. Accepted Manuscript published: November 28, 2018 (version 1)
  4. Version of Record published: December 27, 2018 (version 2)

Copyright

© 2018, Ilca et al.

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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  1. F Tudor Ilca
  2. Andreas Neerincx
  3. Clemens Hermann
  4. Ana Marcu
  5. Stefan Stevanović
  6. Janet E Deane
  7. Louise H Boyle
(2018)
TAPBPR mediates peptide dissociation from MHC class I using a leucine lever
eLife 7:e40126.
https://doi.org/10.7554/eLife.40126

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