Strain, strain background (Mus musculus, females) | CD1/NCrl | Charles River | Strain code: 022 | foster dams for generation of knock-in mice |
Strain, strain background (Mus musculus, females and males) | BXD recombinant inbred strains | University of Tennessee Health Sciences Center | | original breeders from Dr. Robert Williams; offspring for research produced by Dr. Tamara Phillips at VA Portland Health Care System (VAPORHCS) |
Genetic reagent (Mus musculus, females) | MAHDR | VAPORHCS | | methamphetamine high drinking mice; created by Dr. Tamara Phillips; all are homozygous for SNP rs33645709 |
Genetic reagent (Mus musculus, females and males) | MAHDR-Taar1m1J/m1J control | this paper | | created by Oregon Health & Science University Transgenic Mouse Models Shared Resource; offspring for research produced by Dr. Tamara Phillips at VAPORHCS |
Genetic reagent (Mus musculus, females and males) | MAHDR-Taar1+/+ knock-in | this paper | | created by Oregon Health & Science University Transgenic Mouse Models Shared Resource; offspring for research produced by Dr. Tamara Phillips at VAPORHCS |
Biological sample (Mus musculus) | tail snip | other | | obtained from breeders and research animals to determine genotype |
Sequence-based reagent | Taar1 gRNA | this paper | | designed by ThermoFisher Scientific; reagent for generation of knock-in mice (Mus musculus) |
Sequence-based reagent | 100b oligo single strand DNA for Taar1+ (ossDNA) | this paper | MGI:MGI2148258 | synthesized by ThermoFisher Scientific; reagent for generation of knock-in mice (Mus musculus) |
Sequence-based reagent | Cas9 mRNA | TriLink | Catalog number: L-7606 | reagent for generation of knock-in mice (Mus musculus) |
Sequence-based reagent | primers for genotyping Oprm1 | this paper | | original reference for these primers: Ferraro et al. (2005); based on Oprm1 sequence NM_001304955 (Mus musculus) |
Sequence-based reagent | DdeI restriction enzyme | ThermoFisher Scientific | Catalog number: ER1882 | reagent for Oprm1 genotyping (Helicobacter pylori, RFL3) |
Commercial assay or kit | QuickExtract DNA Extraction Solution | Lucigen | Catalog number: QE09050 | |
Commercial assay or kit | Hotstart DNA Polymerase Kit | Qiagen | Catalog number: 203205 | |
Commercial assay or kit | Taqman kit for determining Taar1 alleles | ThermoFisher Scientific | Custom order | probes based on Taar1 sequence: NM_053205.1; SNP rs33645709 |
Chemical compound, drug | ethidium bromide | Sigma Aldrich | Catalog number: E1510 | reagent for Oprm1 genotyping |
Chemical compound, drug | (+)-methamphetamine hydrochloride | Sigma Aldrich | Catalog number: M8750 | |
Chemical compound, drug | sterile 0.9% saline | Baxter Healthcare Corporation | Catalog number: 2F7124 | vehicle for methamphetamine |
Software, algorithm | Statistica | TIBCO Software Inc | | |
Software, algorithm | GeneNetwork | University of Tennessee | RRID:SCR_002388 | www.genenetwork.org |
Software, algorithm | R | The R Foundation for Statistical Computing | RRID:SCR_001905 | www.r-project.org |