1. Neuroscience

A neural mechanism for contextualizing fragmented inputs during naturalistic vision

  1. Daniel Kaiser  Is a corresponding author
  2. Jacopo Turini
  3. Radoslaw M Cichy
  1. University of York, United Kingdom
  2. Freie Universität Berlin, Germany
  3. Goethe-Universität Frankfurt, Germany
  4. Humboldt-Universität Berlin, Germany
  5. Bernstein Center for Computational Neuroscience Berlin, Germany
https://doi.org/10.7554/eLife.48182
Share this article
Cite this article
  1. Daniel Kaiser
  2. Jacopo Turini
  3. Radoslaw M Cichy
(2019)
A neural mechanism for contextualizing fragmented inputs during naturalistic vision
eLife 8:e48182.
https://doi.org/10.7554/eLife.48182
  2. Download BibTeX
  3. Download .RIS

Abstract

With every glimpse of our eyes, we sample only a small and incomplete fragment of the visual world, which needs to be contextualized and integrated into a coherent scene representation. Here we show that the visual system achieves this contextualization by exploiting spatial schemata, that is our knowledge about the composition of natural scenes. We measured fMRI and EEG responses to incomplete scene fragments and used representational similarity analysis to reconstruct their cortical representations in space and time. We observed a sorting of representations according to the fragments' place within the scene schema, which occurred during perceptual analysis in the occipital place area and within the first 200 ms of vision. This schema-based coding operates flexibly across visual features (as measured by a deep neural network model) and different types of environments (indoor and outdoor scenes). This flexibility highlights the mechanism's ability to efficiently organize incoming information under dynamic real-world conditions.

https://doi.org/10.7554/eLife.48182.001

Introduction

During natural vision, the brain continuously receives incomplete fragments of information that need to be integrated into meaningful scene representations. Here, we propose that this integration is achieved through contextualization: the brain uses prior knowledge about where information typically appears in a scene to meaningfully sort incoming information.

A format in which such prior knowledge about the world is represented in the brain is provided by schemata. First introduced to philosophy to explain how prior knowledge enables perception of the world (Kant, 1781), schemata were later adapted by psychology (Barlett, 1932; Piaget, 1926) and computer science (Minsky, 1975; Rumelhart, 1980) as a means to formalize mechanisms enabling natural and artificial intelligence, respectively.

In the narrower context of natural vision, scene schemata represent knowledge about the typical composition of real-world environments (Mandler, 1984). Scene schemata for example entail knowledge about the distribution of objects across scenes, where objects appear in particular locations across the scene and in particular locations with respect to other objects (Kaiser et al., 2019a; Torralba et al., 2006; Võ et al., 2019; Wolfe et al., 2011).

The beneficial role of such scene schemata was first investigated in empirical studies of human memory, where memory performance is boosted when scenes are configured in accordance with the schema (Brewer and Treyens, 1981; Mandler and Johnson, 1976; Mandler and Parker, 1976).

Recently however, it has become clear that scene schemata not only organize memory contents, but also the contents of perception. For example, knowledge about the structure of the world can be used to generate predictions about a scene’s content (Bar, 2009; Henderson, 2017), or to efficiently organize the concurrent representation of multiple scene elements (Kaiser et al., 2014; Kaiser et al., 2019b). This position is reinforced by behavioral studies demonstrating a beneficial role of schema-congruent naturalistic stimuli across a variety of perceptual tasks, such as visual detection (Biederman et al., 1982; Davenport and Potter, 2004; Stein et al., 2015) and visual search (Kaiser et al., 2014; Torralba et al., 2006; Võ et al., 2019).

Here, we put forward a novel function of scene schemata in visual processing: they support the contextualization of fragmented sensory inputs. If sensory inputs are indeed processed in relation to the schema context, scene fragments stemming from similar typical positions within the scene should be processed similarly and fragments stemming from different positions should be processed differently. Therefore, the neural representations of scene fragments should be sorted according to their typical place within the scene.

We tested two hypotheses about this sorting process. First, we hypothesized that this sorting occurs during perceptual scene analysis, which can be spatiotemporally pinpointed to scene-selective cortex (Baldassano et al., 2016; Epstein, 2014) and the first 250 ms of processing (Cichy et al., 2017; Harel et al., 2016). Second, given that schema-related effects in behavioral studies (Mandler and Parker, 1976) are more robustly observed along the vertical dimension, where the scene structure is more rigid (i.e., the sky is almost always above the ground), we hypothesized that the cortical sorting of information should primarily occur along the vertical dimension.

To test these hypotheses, we used a novel visual paradigm in which participants were exposed to fragmented visual inputs, and recorded fMRI and EEG data to resolve brain activity in space and time.

Results

In our study, we experimentally mimicked the fragmented nature of naturalistic visual inputs by dissecting scene images into position-specific fragments. Six natural scene images (Figure 1a) were each split into six equally-sized fragments (three vertical × 2 horizontal), resulting in 36 conditions (six scenes × 6 fragments). In separate fMRI (n = 30) and EEG (n = 20) experiments, participants viewed these fragments at central fixation while performing an indoor/outdoor categorization task to ensure engagement with the stimulus (Figure 1b). Critically, this design allowed us to investigate whether the brain sorts the fragments with respect to their place in the schema in the absence of explicit location differences (Figure 1c).

Figure 1 with 1 supplement see all
Download asset Open asset
Experimental design and rationale of schema-based information sorting.

(a) The stimulus set consisted of six natural scenes (three indoor, three outdoor). Each scene was split into six rectangular fragments. (b) During the fMRI and EEG recordings, participants performed an indoor/outdoor categorization task on individual fragments. Notably, all fragments were presented at central fixation, removing explicit location information. (c) We hypothesized that the visual system sorts sensory input by spatial schemata, resulting in a cortical organization that is explained by the fragments’ within-scene location, predominantly in the vertical dimension: Fragments stemming from the same part of the scene should be represented similarly. Here we illustrate the hypothesized sorting in a two-dimensional space. A similar organization was observed in multi-dimensional scaling solutions for the fragments’ neural similarities (see Figure 1—figure supplement 1 and Video 1). In subsequent analyses, the spatiotemporal emergence of the schema-based cortical organization was precisely quantified using representational similarity analysis (Figure 2).

https://doi.org/10.7554/eLife.48182.002
Video 1
Download asset
Time-resolved MDS visualization of the neural RDMs.

To directly visualize the emergence of schematic coding from the neural data, we performed a multi-dimensional scaling (MDS) analysis, where the time-resolved neural RDMs (averaged across participants) were projected onto a two-dimensional space. The RDM time series was smoothed using a sliding averaging window (15 ms width). Computing MDS solutions across time yielded a movie (5 ms resolution), where fragments travel through an arbitrary space, eventually forming a meaningful organization. Notably, around 200 ms, a division into the three vertical locations can be observed.

https://doi.org/10.7554/eLife.48182.004

To quantify the sorting of fragments during cortical processing we used spatiotemporally resolved representational similarity analysis (Cichy et al., 2014; Kriegeskorte et al., 2008). We first extracted representational dissimilarity matrices (RDMs) from the fMRI and EEG data, which indexed pairwise dissimilarities of the fragments’ neural representations (for details on RDM construction see Figure 2—figure supplement 1). In the fMRI (Figure 2a), we extracted spatially-resolved neural RDMs from scene-selective occipital place area (OPA) and parahippocampal place area (PPA), and from early visual cortex (V1) (for temporal response profiles in these regions see Figure 2—figure supplement 2). In the EEG (Figure 2b), we extracted time-resolved neural RDMs from −200 ms to 800 ms relative to stimulus onset from posterior EEG electrodes (for other electrode groups see Figure 2—figure supplements 3–5).

Figure 2 with 9 supplements see all
Download asset Open asset
Spatial schemata determine cortical representations of fragmented scenes.

(a) To test where and when the visual system sorts incoming sensory information by spatial schemata, we first extracted spatially (fMRI) and temporally (EEG) resolved neural representational dissimilarity matrices (RDMs). In the fMRI, we extracted pairwise neural dissimilarities of the fragments from response patterns across voxels in the occipital place area (OPA), parahippocampal place area (PPA), and early visual cortex (V1). (b) In the EEG, we extracted pairwise dissimilarities from response patterns across electrodes at every time point from −200 ms to 800 ms with respect to stimulus onset. (c) We modelled the neural RDMs with three predictor matrices, which reflected their vertical and horizontal positions within the full scene, and their category (i.e., their scene or origin). (d) The fMRI data revealed a vertical-location organization in OPA, but not V1 and PPA. Additionally, the fragment’s category predicted responses in both scene-selective regions. (e) The EEG data showed that both vertical location and category predicted cortical responses rapidly, starting from around 100 ms. These results suggest that the fragments’ vertical position within the scene schema determines rapidly emerging representations in scene-selective occipital cortex. Significance markers represent p<0.05 (corrected for multiple comparisons). Error margins reflect standard errors of the mean. In further analysis, we probed the flexibility of this schematic coding mechanism (Figure 3).

https://doi.org/10.7554/eLife.48182.005

We then quantified schema effects using separate model RDMs for horizontal and vertical locations (Figure 2c). These location RDMs reflected whether pairs of fragments shared the same location or not. We additionally constructed a category model RDM, which reflected whether pairs of fragments stemmed from the same scene or not.

Critically, if cortical information is indeed sorted with respect to scene schemata, we should observe a neural clustering of fragments that stem from the same within-scene location – in this case, the location RDM should predict a significant proportion of the representational organization in visual cortex.

To test this, we modeled neural RDMs as a function of the model RDMs using general linear models, separately for the fMRI and EEG data. The resulting beta weights indicated to which degree location and category information accounted for cortical responses in the three ROIs and across time.

The key observation was that the fragments’ vertical location predicted neural representations in OPA (t[29] = 4.12, p<0.001, pcorr <0.05), but not in V1 and PPA (test statistics for all analyses and ROIs are reported in Supplementary file 1) (Figure 2d) and between 55 ms and 685 ms (peak: t[19] = 9.03, p<0.001, pcorr <0.05) (Figure 2e). This vertical-location organization was consistent across the first and second half of the experiments (see Figure 2—figure supplement 6) and across all pairwise comparisons along the vertical axis (see Figure 2—figure supplement 7). No effects were observed for horizontal location, consistent with more rigid spatial scene structure in the vertical dimension (Mandler and Parker, 1976). This result provides a first characterization of where and when incoming information is organized in accordance with scene schemata: in OPA and rapidly after stimulus onset, scene fragments are sorted according to their origin within the environment.

The schema-based organization co-exists with a prominent scene-category organization: In line with previous findings (Lowe et al., 2018; Walther et al., 2009), category was accurately predicted in OPA (t[29] = 3.12, p=0.002, pcorr <0.05) and PPA (t[29] = 4.26, p<0.001, pcorr <0.05) (Figure 2d), and from 60 ms to 775 ms (peak: t[19] = 6.39, p<0.001, pcorr <0.05) (Figure 2e).

To efficiently support vision in dynamic natural environments, schematic coding needs to be flexible with respect to visual properties of specific scenes. The absence of vertical location effects in V1 indeed highlights that schematic coding is not tied to the analysis of simple visual features. To more thoroughly probe this flexibility, we additionally conducted three complementary analyses (Figure 3).

Figure 3 with 3 supplements see all
Download asset Open asset
Schematic coding operates flexibly across visual and conceptual scene properties.

(a) To determine the role of categorization-related visual features in this schematic organization, we regressed out RDMs obtained from 18 layers along the ResNet50 DNN before repeated the three-predictor general linear model (GLM) analysis (Figure 2c). (b/c) Removing DNN features abolished category information in fMRI and EEG signals, but not vertical location information. (d) To test for generalization across different scene types, we restricted location predictor RDMs to comparisons across indoor and outdoor scenes. Due to this restriction, category could not be modelled. (e/f) In this analysis, vertical location still predicted neural organization in OPA and from 70 ms. (g) Finally, we combined the two analyses: we first regressed out DNN features prior and then modelled the neural RDMs using the restricted predictor RDMs (d). (h) In this analysis, we still found significant vertical location information in OPA. (i) Notably, vertical location information in the EEG signals was delayed to after 180 ms, suggesting that at this stage schematic coding becomes flexible to visual and conceptual attributes. Significance markers represent p<0.05 (corrected for multiple comparisons). Error margins reflect standard errors of the mean.

https://doi.org/10.7554/eLife.48182.015

First, we tested whether schematic coding is tolerant to stimulus features relevant for visual categorization. Categorization-related features were quantified using a deep neural network (DNN; ResNet50), which extracts such features similarly to the brain (Wen et al., 2018). We removed DNN features by regressing out layer-specific RDMs constructed from DNN activations (see Materials and Methods for details) (Figure 3a); subsequently, we re-estimated location and category information.

After removing DNN features, category information was rendered non-significant in both fMRI and EEG signals. When directly comparing category information before and after removing the DNN features, we found reduced category information in PPA (t[29] = 2.48, p = 0.010, pcorr <0.05) and OPA (t[29] = 1.86, p = 0.036, pcorr >0.05), and a strong reduction of category information across time, from 75 ms to 775 ms (peak t[19] = 13.0, p<0.001, pcorr <0.05). Together, this demonstrates that categorization-related brain activations are successfully explained by DNN features (Cichy et al., 2016; Cichy et al., 2017; Groen et al., 2018; Güçlü and van Gerven, 2015; Wen et al., 2018), indicating the appropriateness of our DNN for modelling visual brain activations. Despite the suitability of our DNN model for modelling categorical brain responses, vertical location still accounted for the neural organization in OPA (t[29] = 2.37, p = 0.012, pcorr <0.05) (Figure 3b) and between 75 ms and 335 ms (peak: t[19] = 5.06, p<0.001, pcorr <0.05) (Figure 3c). Similar results were obtained using a shallower feed-forward DNN (see Figure 3—figure supplement 1). This result suggests that schematic coding cannot be explained by categorization-related features extracted by DNN models.

DNN features are a useful control for flexibility regarding visual features, because they cover both low-level and high-level features, explaining variance across fMRI regions and across EEG processing time (see Figure 3—figure supplement 2; see also Cichy et al., 2016; Güçlü and van Gerven, 2015). However, to more specifically control for low-level features, we used two commonly employed low-level control models: pixel dissimilarity and GIST descriptors (Oliva and Torralba, 2001). These models neither explained the vertical location organization nor the category organization in the neural data (see Figure 3—figure supplement 3). Finally, as an even stronger control of the low-level features encoded in V1, we used the neural dissimilarity structure in V1 (i.e., the neural RDMs) as a control model, establishing an empirical neural measure of low-level features. With V1 housing precise low-level feature representations, this measure should very well capture the features extracted during the early processing of simple visual features. However, removing the V1 dissimilarity structure did neither abolish the schematic coding effects in the OPA nor in the EEG data (see Figure 3—figure supplement 3). This shows that even if we had control models that approximated V1 representations extremely well – as well as the V1 representations approximate themselves – these models could not explain vertical location effects in downstream processing. Together, these results provide converging evidence that low-level feature processing cannot explain the schematic coding effects reported here.

Second, we asked whether schematic coding operates flexibly across visually diverse situations. To test this explicitly we restricted RDMs to comparisons between indoor and outdoor scenes, which vary substantially in visual characteristics (Torralba and Oliva, 2003) (Figure 3d).

Vertical location still predicted cortical organization in OPA (t[29] = 3.05, p = 0.002, pcorr <0.05) (Figure 3e) and from 70 ms to 385 ms (peak: t[19] = 7.47, p<0.001, pcorr <0.05) (Figure 3f). The generalization across indoor and outdoor scenes indicates that schematic coding operates similarly across radically different scenes, suggesting that the mechanism can similarly contextualize information across different real-life situations.

Finally, for a particularly strong test of flexibility, we tested for schematic coding after removing both DNN features and within-category comparisons (Figure 3g). In this analysis, OPA representations were still explained by the fragments’ vertical location (t[29] = 2.38, p = 0.012, pcorr <0.05) (Figure 3h). Notably, early schema effects were rendered non-significant, while vertical location still predicted representations after 180 ms (peak: t[19] = 4.41, p<0.001, pcorr <0.05) (Figure 3i), suggesting a high degree of flexibility emerging at that time. Interestingly, across all analyses, vertical location information was exclusively found in OPA and always peaked shortly after 200 ms (see Supplementary file 2), suggesting that schematic coding occurs during early perceptual analysis of scenes.

Discussion

Together, our findings characterize a novel neural mechanism for contextualizing fragmented inputs during naturalistic vision. The mechanism exploits schemata to sort sensory inputs into meaningful representations of the environment. This sorting occurs during perceptual scene analysis in scene-selective OPA and within the first 200 ms of vision, and operates flexibly across changes in visual properties.

That schema-based coding can be localized to OPA is consistent with the region’s important role in visual scene processing. Transcranial magnetic stimulation studies suggest that OPA activation is crucial for various scene perception tasks, such as scene discrimination (Dilks et al., 2013; Ganaden et al., 2013), navigating through scenes (Julian et al., 2016) and anticipating upcoming scene information (Gandolfo and Downing, 2019). Functional MRI work suggest that computations in the OPA include the analysis of spatial scene layout (Dillon et al., 2018; Henriksson et al., 2019; Lowe et al., 2017) and the parsing of local scene elements like objects and local surfaces (Kamps et al., 2016). Future studies are needed to clarify which of these computations mediate the schema-based coding described here.

As the current study is limited to a small set of scenes, more research is needed to explore whether schema-based coding generalizes to more diverse contents. It is conceivable that schema-based coding constitutes a more general coding strategy that may generalize to other visual contents (such as faces; Henriksson et al., 2015) and non-visual processing domains: when sensory information is fragmented and spatial information is unreliable, the brain may use schematic information to contextualize sensory inputs. This view is in line with Bayesian theories of perception where the importance of prior information for perceptual inference grows with the noisiness and ambiguity of the sensory information at hand (Ernst and Banks, 2002; Kersten et al., 2004).

The schema-based sorting of scene representations provides a mechanism for efficient communication between perceptual and cognitive systems: when scene information is formatted with respect to its role in the environment, it can be efficiently read out by downstream processes. This idea is consistent with the emerging view that cortical representations depend on functional interactions with the environment (Bonner and Epstein, 2017; Groen et al., 2018; Malcolm et al., 2016; Peelen and Downing, 2017). Under this view, formatting perceptual information according to real-world structure may allow cognitive and motor systems to efficiently read out visual information that is needed for different real-world tasks (e.g., immediate action versus future navigation). As the schema-based sorting of scene information happens already during early scene analysis, many high-level processes have access to this information.

Lastly, our results have implications for computational modelling of vision. While DNNs trained on categorization accurately capture the representational divide into different scene categories, they cannot explain the schema-based organization observed in the human visual system. Although this does not mean that visual features extracted by DNN models in principle are incapable of explaining schema-based brain representations, our results highlight that current DNN models of categorization do not use real-world structure in similar ways as the human brain. In the future, augmenting DNN training procedures with schematic information (Katti et al., 2019) may improve their performance on real-world tasks and narrow the gap between artificial and biological neural networks.

To conclude, our findings provide the first spatiotemporal characterization of a neural mechanism for contextualizing fragmented visual inputs. By rapidly organizing visual information according to its typical role in the world, this mechanism may contribute to the optimal use of perceptual information for guiding efficient real-world behaviors, even when sensory inputs are incomplete or dynamically changing.

Materials and methods

Key resources table
Reagent type
(species) or resource		DesignationSource or referenceIdentifiersAdditional
information
Software, algorithmCoSMoMVPAOosterhof et al., 2016RRID:SCR_014519For data analysis
Software, algorithmfieldtripOostenveld et al., 2011RRID:SCR_004849For EEG data preprocessing
Software, algorithmMATLABMathworks Inc.RRID:SCR_001622For stimulus delivery and data analysis
Software, algorithmPsychtoolbox 3Brainard, 1997RRID:SCR_002881For stimulus delivery
Software, algorithmSPM12www.fil.ion.ucl.ac.uk/spm/software/spm12/RRID:SCR_007037For fMRI data preprocessing

Participants

Thirty adults (mean age 23.9 years, SD = 4.4; 26 females) completed the fMRI experiment and twenty (mean age 24.0 years, SD = 4.3; 15 females) completed the EEG experiment. All participants had normal or corrected-to-normal vision. They all provided informed consent and received monetary reimbursement or course credits for their participation. All procedures were approved by the ethical committee of the Department of Education and Psychology at Freie Universität Berlin (reference 140/2017) and were in accordance with the Declaration of Helsinki.

Stimuli

Request a detailed protocol

The stimulus set (Figure 1a) consisted of fragments taken from three images of indoor scenes (bakery, classroom, kitchen) and three images of outdoor scenes (alley, house, farm). Each image was split horizontally into two halves, and each of the halves was further split vertically in three parts, so that for each scene six fragments were obtained. Participants were not shown the full scene images prior to the experiment.

Experimental design

Request a detailed protocol

The fMRI and EEG designs were identical, unless otherwise noted. Stimulus presentation was controlled using the Psychtoolbox (Brainard, 1997; RRID:SCR_002881). In each trial, one of the 36 fragments was presented at central fixation (7° horizontal visual angle) for 200 ms (Figure 1b). Participants were instructed to maintain central fixation and categorize each stimulus as an indoor or outdoor scene image by pressing one of two buttons.

In the fMRI experiment, the inter-trial interval was kept constant at 2,300 ms, irrespective of the participant’s response time. In the EEG experiment, after each response a green or red fixation dot was presented for 300 ms to indicate response correctness; participants were instructed to only blink after the feedback had occurred. Trials were separated by a fixation interval randomly varying between 1500 ms and 2000 ms.

In the fMRI, participants performed six identical runs. Within each run, each of the 36 scene fragments was shown four times, resulting in 144 trials. Additionally, each run contained 29 fixation trials, where only the central fixation dot was shown. Runs started and ended with brief fixation periods; the total run duration was 7:30 min. In the EEG, each of the 36 fragments was presented 40 times during the experiment, for a total of 1440 trials, divided into 10 runs. Three participants performed a shorter version of the experiment, with only 20 repetitions of each image (720 trials in total).

In both experiments, participants performed very well in the indoor/outdoor categorization task (fMRI: 94% correct, 658 ms mean response time, EEG: 96%, 606 ms). Differences in task difficulty across fragments were not related to the neural effects of interest (Figure 2—figure supplement 8).

fMRI recording and preprocessing

Request a detailed protocol

MRI data was acquired using a 3T Siemens Tim Trio Scanner equipped with a 12-channel head coil. T2*-weighted gradient-echo echo-planar images were collected as functional volumes (TR = 2 s, TE = 30 ms, 70° flip angle, 3 mm3 voxel size, 37 slices, 20% gap, 192 mm FOV, 64 × 64 matrix size, interleaved acquisition). Additionally, a T1-weighted image (MPRAGE; 1 mm3 voxel size) was obtained as a high-resolution anatomical reference. During preprocessing, the functional volumes were realigned and coregistered to the T1 image, using MATLAB (RRID:SCR_014519) and SPM12 (www.fil.ion.ucl.ac.uk/spm/; RRID:SCR_014519).

fMRI region of interest definition

Request a detailed protocol

We restricted our analyses to three regions of interest (ROIs). We defined scene-selective occipital place area (OPA; Dilks et al., 2013) and parahippocampal place area (PPA; Epstein and Kanwisher, 1998) using a functional group atlas (Julian et al., 2012). As a control region, we defined early visual cortex (V1) using a probabilistic atlas (Wang et al., 2015). All ROIs were defined in standard space and then inverse-normalized into individual-participant space. For each ROI, we concatenated the left- and right-hemispheric masks and performed analyses on the joint ROI.

EEG recording and preprocessing

Request a detailed protocol

The EEG was recorded using an EASYCAP 64-channel system and a Brainvision actiCHamp amplifier. The electrodes were arranged in accordance with the standard 10–10 system. The data was recorded at a sampling rate of 1000 Hz and filtered online between 0.03 Hz and 100 Hz. All electrodes were referenced online to the Fz electrode. Offline preprocessing was performed in MATLAB, using the FieldTrip toolbox (Oostenveld et al., 2011; RRID:SCR_004849). The continuous EEG data were epoched into trials ranging from 200 ms before stimulus onset to 800 ms after stimulus onset, and baseline corrected by subtracting the mean of the pre-stimulus interval for each trial and channel separately. Trials containing movement-related artefacts were automatically identified and removed using the default automatic rejection procedure implemented in Fieldtrip. Channels containing excessive noise were removed based on visual inspection. Blinks and eye movement artifacts were identified and removed using independent components analysis and visual inspection of the resulting components. The epoched data were down-sampled to 200 Hz.

Representational similarity analysis

To model the representational structure of the neural activity related to our stimulus set, we used representational similarity analysis (RSA; Kriegeskorte et al., 2008). We first extracted neural RDMs separately for the fMRI and EEG experiments, and then used the same analyses to model their organization. To retrieve the fragments’ position within the original scene, as well their scene category, we used a regression approach, where we modeled neural dissimilarity as a linear combination of multiple predictors (Proklova et al., 2016; Proklova et al., 2019).

Constructing neural dissimilarity – fMRI

Request a detailed protocol

For the fMRI data, we used cross-validated correlations as a measure of pairwise neural dissimilarity. First, patterns for each ROI were extracted from the functional images corresponding to the trials of interest. After shifting the activation time course by 3 TRs (i.e., 6 s, accounting for the hemodynamic delay), we extracted voxel-wise activation values for each trial, from the TR that was closest to the stimulus onset on this trial (for results across 6 TRs with respect to trial onset, see Figure 2—figure supplement 2). To account for activation differences between runs, the mean activation across conditions was subtracted from each voxel’s values, separately for each run. For each ROI, response patterns across voxels were used to perform multivariate analyses using the CoSMoMVPA toolbox (Oosterhof et al., 2016; RRID:SCR_014519). For each TR separately, we performed correlation-based (Haxby et al., 2001) multi-voxel pattern analyses (MVPA) for each pair of fragments. These analyses were cross-validated by repeatedly splitting the data into two equally-sized sets (i.e., half of the runs per set). For this analysis, we correlated the patterns across the two sets, both within-condition (i.e., the patterns stemming from the two same fragments and from different sets) and between-conditions (i.e., the patterns stemming from the two different fragments and from different sets). These correlations were Fisher-transformed. Then, we subtracted the within- and between-correlations to obtain a cross-validated correlation measure, where above-zero values reflect successful discrimination. This procedure was repeated for all possible splits of the six runs. Performing this MVPA for all pairs of fragments yielded a 36 × 36 representational dissimilarity matrix (RDM) for each ROI. RDMs’ entries reflected the neural dissimilarity between pairs of fragments (the diagonal remained empty).

Constructing neural dissimilarity – EEG

Request a detailed protocol

For the EEG data, we used cross-validated classification accuracies as a measure of pairwise neural dissimilarity. We thus constructed RDMs across time by performing time-resolved multivariate decoding analyses (Contini et al., 2017). RDMs were built by computing pair-wise decoding accuracy for all possible combinations of the 36 stimuli, using the CoSMoMVPA toolbox (Oosterhof et al., 2016). As we expected the highest classification in sensors over visual cortex (Battistoni et al., 2018; Kaiser et al., 2016), only 17 occipital and posterior sensors (O1, O2, Oz, PO3, PO4, PO7, PO8, POz, P1, P2, P3, P4, P5, P6, P7, P8, Pz) were used in this analysis. We report results for other electrode groups in Figure 2—figure supplements 3–5. For each participant, classification was performed separately for each time point across the epoch (i.e., with 5 ms resolution). The analysis was performed in a pair-wise fashion: Linear discriminant analysis classifiers were always trained and tested on data from two conditions (e.g., the middle left part of the alley versus the top right part of the farm), using a leave-one-trial-out partitioning scheme. The training set consisted of all but one trials for each of the two conditions, while one trial for each of the two conditions was held back and used for classifier testing. This procedure was repeated until every trial was left out once. Classifier performance was averaged across these repetitions. The pairwise decoding analysis resulted in a 36-by-36 neural RDM for each time point. A schematic description of the RDM construction can be found in Figure 2—figure supplement 1.

Location and category predictors

Request a detailed protocol

We predicted the neural RDMs in a general linear model (GLM; see below) with three different predictor RDMs (36 × 36 entries each) (Figure 2c): In the vertical location RDM, each pair of conditions is assigned either a value of 0, if the fragments stem from the same vertical location, or the value 1, if they stem from different vertical locations (for results with an alternative predictor RDM using Euclidean distances see Figure 2—figure supplement 9). In the horizontal location RDM, each pair of conditions is assigned either a value of 0, if the fragments stem from the same horizontal location, or a value of 1, if they stem from different horizontal locations. In the category RDM, each pair of conditions is assigned either a value of 0, if the fragments stem from the same scene, or a value of 1, if they stem from different scenes.

In an additional analysis, we sought to eliminate properties specific to either the indoor or outdoor scenes, respectively. We therefore constructed RDMs for horizontal and vertical location information which only contained comparisons between the indoor and outdoor scenes. These RDMs were constructed in the same way as explained above, but all comparisons within the same scene type of scene were removed (Figure 3d).

Modelling neural dissimilarity

Request a detailed protocol

To reveal correspondences between the neural data and the predictor matrices, we used GLM analyses. Separately for each ROI (fMRI) or time point (EEG), we modelled the neural RDM as a linear function of the vertical location RDM, the horizontal location RDM, and the category RDM. Prior to each regression, the neural RDMs and predictor RDMs were vectorized by selecting all lower off-diagonal elements – the rest of the entries, including the diagonal, was discarded. Values for the neural RDMs were z-scored. Separately for each subject and each time point, three beta coefficients (i.e., regression weights) were estimated. By averaging across participants, we obtained time-resolved beta estimates for each predictor, showing how well each predictor explains the neural data over time.

Furthermore, we performed an additional GLM analysis with a vertical location predictor and a horizontal location predictor, where comparisons within indoor- and outdoor-scenes were removed (Figure 3d–f); these comparisons were also removed from the regression criterion. Using the same procedure as in the previous GLM analysis, we then estimated the beta coefficients for each predictor at each time point, separately for each subject. For this analysis, a category RDM could not be constructed, as all comparisons of fragments from the same scene were eliminated.

Controlling for deep neural network features

Request a detailed protocol

To control for similarity in categorization-related visual features, we used a deep neural network (DNN) model. DNNs have recently become the state-of-the-art model of visual categorization, as they tightly mirror the neural organization of object and scene representations (Cichy et al., 2016; Cichy et al., 2017; Cichy and Kaiser, 2019; Groen et al., 2018; Güçlü and van Gerven, 2015; Wen et al., 2018). DNNs are similar to the brain as they are trained using excessive training material while dynamically adjusting the ‘tuning’ of their connections. Here, we used a DNN (see below) that has been trained to categorize objects across a large number of images and categories, therefore providing us with a high-quality model of how visual features are extracted for efficient categorization. By comparing DNNs activations and brain responses to the scene fragments, we could quantify to which extent features routinely extracted for categorization purposes account for schema-based coding in the human visual system.

In a two-step approach, we re-performed our regression analysis after removing the representational organization emerging from the DNN. First, we used a regression model to remove the contribution of the dissimilarity structure in the DNN model. This model included one predictor for each layer extracted from the DNN (i.e., one RDM for each processing step along the DNN). Estimating this model allowed us to remove the neural organization explained by the DNN while retaining what remains unexplained (in the regression residuals). Second, we re-ran the previous regression analyses (see above), but now the residuals of the DNN regression were used as the regression criterion, so that only the organization that remained unexplained by the DNN was modeled.

As a DNN model, we used a pre-trained version (trained on image categorization for the ImageNet challenge) of the ResNet50 model (He et al., 2016), as implemented in MatConvNet (Vedaldi and Lenc, 2015). This model’s deeper, residual architecture outperforms shallower models in approximating visual cortex organization (Wen et al., 2018). ResNet50 consists of 16 blocks of residual layer modules, where information both passes through an aggregate of layers within the block, and bypasses the block; then the residual between the processed and the bypassing information is computed. Additionally, ResNet50 has one convolutional input layer, and one fully-connected output layer. Here, to not inflate the number of intercorrelated predictor variables, we only used the final layer of each residual block, and thus 18 layers in total (16 from the residual blocks, and the input and output layers). For each layer, an RDM was built using 1-correlation between the activations of all nodes in the layer, separately for each pair of conditions. For regressing out the DNN RDMs, we added one predictor for each available RDM. In Figure 3—figure supplement 1, we show that an analysis using the AlexNet architecture (Krizhevsky et al., 2012) yields comparable results; in Figure 3—figure supplement 2, we additionally provide information about the DNN model fit across regions and time points.

Statistical testing

Request a detailed protocol

For the fMRI data, we tested the regression coefficients against zero, using one-tailed, one-sample t-tests (i.e., testing the hypothesis that coefficients were greater than zero). Multiple-comparison correction was based on Bonferroni-corrections across ROIs. A complete report of all tests performed on the fMRI data can be found in Supplementary file 1. For the EEG data, we used a threshold-free cluster enhancement procedure (Smith and Nichols, 2009) to identify significant effects across time. Multiple-comparison correction was based on a sign-permutation test (with null distributions created from 10,000 bootstrapping iterations) as implemented in CoSMoMVPA (Oosterhof et al., 2016). The resulting statistical maps were thresholded at Z > 1.64 (i.e., p<0.05, one-tailed against zero). Additionally, we report the results of one-sided t-tests for all peaks effects. To estimate the reliability of onset and peak latencies we performed bootstrapping analyses, which are reported in Supplementary Items 2/3.

Data availability

Request a detailed protocol

Data are publicly available on OSF (DOI.ORG/10.17605/OSF.IO/H3G6V).

Data availability

Data are publicly available on OSF (http://doi.org/10.17605/OSF.IO/H3G6V), as indicated in the Materials and Methods section of the manuscript.

The following data sets were generated
    1. Kaiser D
    2. Turini J
    3. Cichy RM
    (2019) Open Science Framwork
    A neural mechanism for contextualizing fragmented information during naturalistic vision.
    http://doi.org/10.17605/OSF.IO/H3G6V

References

    1. Bar M
    (2009) The proactive brain: memory for predictions
    Philosophical Transactions of the Royal Society B: Biological Sciences 364:1235–1243.
    https://doi.org/10.1098/rstb.2008.0310
  3. Book
    1. Barlett FC
    (1932)
    Remembering: A Study in Experimental and Social Psychology
    Cambridge University Press.
    1. Epstein RA
    (2014)
    Scene Vision
    Neural systems for visual scene recognition, Scene Vision, MIT Press.
  26. Conference
    1. He K
    2. Zhang X
    3. Ren S
    4. Sun J
    (2016) Deep residual learning for image recognition
    Proceedings of the IEEE Conference on Computer Vision and Pattern Recognition. pp. 770–778.
    https://doi.org/10.1109/cvpr.2016.90
  37. Book
    1. Kant I
    (1781)
    Kritik Der Reinen Vernunf
    Johann Friedrich Hartknoch.
  45. Book
    1. Mandler JM
    (1984)
    Stories, Scripts and Scenes: Aspects of Schema Theory
    Taylor & Francis.
    1. Mandler JM
    2. Johnson NS
    (1976) Some of the thousand words a picture is worth
    Journal of Experimental Psychology: Human Learning and Memory 2:529–540.
    https://doi.org/10.1037/0278-7393.2.5.529
    1. Minsky M
    (1975)
    The Psychology of Computer Vision
    A framework for representing knowledge, The Psychology of Computer Vision, McGraw-Hill.
  53. Book
    1. Piaget J
    (1926)
    The Language and Thought of the Child
    Keagan Paul C, editors. Trench Trubner & Co.
    1. Rumelhart DE
    (1980)
    Theoretical Issues in Reading Comprehension
    Schemata: the building blocks of cognition, Theoretical Issues in Reading Comprehension, CRC press.
  61. Conference
    1. Vedaldi A
    2. Lenc K
    (2015)
    MatConvNet – convolutional neural networks for Matlab
    ACM International Conference on Multimedia.

Article and author information

Author details

  1. Daniel Kaiser

    1. Department of Psychology, University of York, York, United Kingdom
    2. Department of Education and Psychology, Freie Universität Berlin, Berlin, Germany
    Contribution
    Conceptualization, Resources, Data curation, Software, Formal analysis, Supervision, Funding acquisition, Validation, Investigation, Visualization, Methodology, Writing—original draft, Project administration, Writing—review and editing
    For correspondence
    danielkaiser.net@gmail.com
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-9007-3160

  2. Jacopo Turini

    1. Department of Education and Psychology, Freie Universität Berlin, Berlin, Germany
    2. Institute of Psychology, Goethe-Universität Frankfurt, Frankfurt am Main, Germany
    Contribution
    Formal analysis, Investigation, Methodology, Writing—review and editing
    Competing interests
    No competing interests declared

  3. Radoslaw M Cichy

    1. Department of Education and Psychology, Freie Universität Berlin, Berlin, Germany
    2. Berlin School of Mind and Brain, Humboldt-Universität Berlin, Berlin, Germany
    3. Bernstein Center for Computational Neuroscience Berlin, Berlin, Germany
    Contribution
    Conceptualization, Supervision, Funding acquisition, Project administration, Writing—review and editing
    Competing interests
    No competing interests declared

Funding

Deutsche Forschungsgemeinschaft (KA4683/2-1)

  • Daniel Kaiser

Deutsche Forschungsgemeinschaft (CI241/1-1)

  • Radoslaw M Cichy

Deutsche Forschungsgemeinschaft (CI241/3-1)

  • Radoslaw M Cichy

H2020 European Research Council (ERC-2018-StG 803370)

  • Radoslaw M Cichy

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Acknowledgements

DK and RMC are supported by Deutsche Forschungsgemeinschaft (DFG) grants (KA4683/2-1, CI241/1-1, CI241/3-1). RMC is supported by a European Research Council Starting Grant (ERC-2018-StG).

Ethics

Human subjects: All participants provided informed written consent. All procedures were approved by the ethical committee of the Department of Education and Psychology at Freie Universität Berlin (reference 140/2017) and were in accordance with the Declaration of Helsinki.

Copyright

© 2019, Kaiser et al.

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

Metrics

  • 1,917
    views
  • 275
    downloads
  • 24
    citations

Views, downloads and citations are aggregated across all versions of this paper published by eLife.

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Open citations (links to open the citations from this article in various online reference manager services)

Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)

  1. Daniel Kaiser
  2. Jacopo Turini
  3. Radoslaw M Cichy
(2019)
A neural mechanism for contextualizing fragmented inputs during naturalistic vision
eLife 8:e48182.
https://doi.org/10.7554/eLife.48182

Share this article

https://doi.org/10.7554/eLife.48182

Categories and tags

Research organism

Further reading

    1. Neuroscience

    Single-cell transcriptomics of vomeronasal neuroepithelium reveals a differential endoplasmic reticulum environment amongst neuronal subtypes

    GVS Devakinandan, Mark Terasaki, Adish Dani
    Research Article

    Specialized chemosensory signals elicit innate social behaviors in individuals of several vertebrate species, a process that is mediated via the accessory olfactory system (AOS). The AOS comprising the peripheral sensory vomeronasal organ has evolved elaborate molecular and cellular mechanisms to detect chemo signals. To gain insight into the cell types, developmental gene expression patterns, and functional differences amongst neurons, we performed single-cell transcriptomics of the mouse vomeronasal sensory epithelium. Our analysis reveals diverse cell types with gene expression patterns specific to each, which we made available as a searchable web resource accessed from https://www.scvnoexplorer.com. Pseudo-time developmental analysis indicates that neurons originating from common progenitors diverge in their gene expression during maturation with transient and persistent transcription factor expression at critical branch points. Comparative analysis across two of the major neuronal subtypes that express divergent GPCR families and the G-protein subunits Gnai2 or Gnao1, reveals significantly higher expression of endoplasmic reticulum (ER) associated genes within Gnao1 neurons. In addition, differences in ER content and prevalence of cubic membrane ER ultrastructure revealed by electron microscopy, indicate fundamental differences in ER function.

    1. Cell Biology
    2. Neuroscience

    Projection neurons are necessary for the maintenance of the mouse olfactory circuit

    Luis Sánchez-Guardado, Peyman Callejas Razavi ... Carlos Lois
    Research Article

    The assembly and maintenance of neural circuits is crucial for proper brain function. Although the assembly of brain circuits has been extensively studied, much less is understood about the mechanisms controlling their maintenance as animals mature. In the olfactory system, the axons of olfactory sensory neurons (OSNs) expressing the same odor receptor converge into discrete synaptic structures of the olfactory bulb (OB) called glomeruli, forming a stereotypic odor map. The OB projection neurons, called mitral and tufted cells (M/Ts), have a single dendrite that branches into a single glomerulus, where they make synapses with OSNs. We used a genetic method to progressively eliminate the vast majority of M/T cells in early postnatal mice, and observed that the assembly of the OB bulb circuits proceeded normally. However, as the animals became adults the apical dendrite of remaining M/Ts grew multiple branches that innervated several glomeruli, and OSNs expressing single odor receptors projected their axons into multiple glomeruli, disrupting the olfactory sensory map. Moreover, ablating the M/Ts in adult animals also resulted in similar structural changes in the projections of remaining M/Ts and axons from OSNs. Interestingly, the ability of these mice to detect odors was relatively preserved despite only having 1–5% of projection neurons transmitting odorant information to the brain, and having highly disrupted circuits in the OB. These results indicate that a reduced number of projection neurons does not affect the normal assembly of the olfactory circuit, but induces structural instability of the olfactory circuitry of adult animals.

    1. Medicine
    2. Neuroscience

    Human birth tissue products as a non-opioid medicine to inhibit post-surgical pain

    Chi Zhang, Qian Huang ... Yun Guan
    Research Article

    Pain after surgery causes significant suffering. Opioid analgesics cause severe side effects and accidental death. Therefore, there is an urgent need to develop non-opioid therapies for managing post-surgical pain. Local application of Clarix Flo (FLO), a human amniotic membrane (AM) product, attenuated established post-surgical pain hypersensitivity without exhibiting known side effects of opioid use in mice. This effect was achieved through direct inhibition of nociceptive dorsal root ganglion (DRG) neurons via CD44-dependent pathways. We further purified the major matrix component, the heavy chain-hyaluronic acid/pentraxin 3 (HC-HA/PTX3) from human AM that has greater purity and water solubility than FLO. HC-HA/PTX3 replicated FLO-induced neuronal and pain inhibition. Mechanistically, HC-HA/PTX3-induced cytoskeleton rearrangements to inhibit sodium current and high-voltage activated calcium current on nociceptive DRG neurons, suggesting it is a key bioactive component mediating pain relief. Collectively, our findings highlight the potential of naturally derived biologics from human birth tissues as an effective non-opioid treatment for post-surgical pain. Moreover, we unravel the underlying neuronal mechanisms of pain inhibition induced by FLO and HC-HA/PTX3.