The structure of the yeast Ctf3 complex
- Howard Hughes Medical Institute, Harvard Medical School, United States
- Harvard University, United States
Abstract
Kinetochores are the chromosomal attachment points for spindle microtubules. They are also signaling hubs that control major cell cycle transitions and coordinate chromosome folding. Most well-studied eukaryotes rely on a conserved set of factors, which are divided among two loosely-defined groups, for these functions. Outer kinetochore proteins contact microtubules or regulate this contact directly. Inner kinetochore proteins designate the kinetochore assembly site by recognizing a specialized nucleosome containing the H3 variant Cse4/CENP-A. We previously determined the structure, resolved by cryo-electron microscopy (cryo-EM), of the yeast Ctf19 complex (Ctf19c, homologous to the vertebrate CCAN), providing a high-resolution view of inner kinetochore architecture (Hinshaw and Harrison, 2019). We now extend these observations by reporting a near-atomic model of the Ctf3 complex, the outermost Ctf19c sub-assembly seen in our original cryo-EM density. The model is sufficiently well-determined by the new data to enable molecular interpretation of Ctf3 recruitment and function.
Data availability
The final Ctf3c cryo-EM map and the soft mask used for three-dimensional refinements of fully unbinned particles have been deposited with accession code EMD-20200. The refined Ctf3c model has been deposited with accession code 6OUA.
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Cryo-EM structure of the yeast Ctf3 complexProtein Data Bank, 6OUA.
Article and author information
Author details
Stephen C Harrison
Funding
Howard Hughes Medical Institute
- Stephen M Hinshaw
Howard Hughes Medical Institute
- Stephen C Harrison
Helen Hay Whitney Foundation
- Stephen M Hinshaw
National Institutes of Health (5T32GM095450-08)
- Andrew N Dates
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2019, Hinshaw et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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The structure of the yeast Ctf3 complex
eLife 8:e48215.
https://doi.org/10.7554/eLife.48215
