(A) Histogram of missense mutations and others (nonsense, frameshift, in-frame indels, and splice site mutations) across the entire MAGEA family. (B) Mutation frequency analysis of MAGEA genes. Pink …
(A) Immunoblot analysis of MAGEA3, A4, A6 and A10 variants expressed in HEK293T cells. Variants that are not recognized by the antibody (gray), variants expressed at levels 33% (three standard …
MAGEA12 and the non-specific band recognized by MAGEA12 antibody are indicated as ← and *, respectively.
Standard deviation of all 15 samples = 0.1118.
Wild-type amino acids of recurrent mutations are shown and colored as in Figure 2A.
(A) Correlation study of protein vs. mRNA expression of MAGEA variants. MAGEA variant protein and mRNA expression levels shown in Figure 3B and Figure 3—figure supplement 1, respectively, were …
qRT-PCR primer RT#3 was used, and MAGEA expression is normalized to that of parental HEK293T cells. *P value < 0.05; two-tailed unpaired t-test.
Each dot represents a variant.
(A) Immunoblots (left) and qRT-PCR (right) analysis of HPDE-iKRAS cells expressing GFP (Vec) and MAGEA6 variants with or without MG132. WT: wild-type, GAPDH: glyceraldehyde 3-phosphate …
Immunoblot (left) and qRT-PCR (right) analysis of MAGEA6 in BxPC-3 (A) and MIA PaCa-2 (B) in the presence or absence of MG132.
Immunoblots of V5-tag pulled-down samples (left) and total lysate (right) are shown.
(A) Cell proliferation analysis of HPDE-iKRAS cells (mean ± standard deviation of replicates, N = 3). (B) Immunoblot study of the transduced HPDE-iKRAS cells with the estimated cell confluency …
Immunoblot analysis of MAGEA6 in transduced PDAC lines AsPC-1 (left), BxPC-3 (middle), and MIA PaCa-2 (right) in nutrient-depleted DMEM (-) with or without MG132. Fully nutrient-supplemented DMEM …
HPDE-iKRAS cells expressing wild-type MAGEA6 were treated with AKT and mTORC1 inhibitors (MK2206 and rapamycin, respectively) as indicated. Immunoblots of AKT and S6K phosphorylation status …
(A) Immunoblot analysis of autophagy signaling in HPDE-iKRAS cells expressing GFP (Vec) and MAGEA6 variants. (B) Immunofluorescence staining of LC3B puncta in the transduced HPDE-iKRAS cells. …
(A) Tumor volume plot (50 d after injection) and Kaplan-Meier survival plot (B) of xenograft assay using HPDE-iKRAS cells expressing GFP (Vec) or MAGEA6 (five mice, two injections each, N = 10). P …
qRT-PCR of endogenous MAGEA6 expression (A) and immunoblot analysis of endogenous p53 (B) in HPDE and six other PDAC cell lines.
(A) qRT-PCR analysis of MAGEA6 expression of the injected BxPC-3 cells in Figure 6C. P value was calculated by two-tailed unpaired t-test. (B) qRT-PCR analysis of MAGEA6, ATG7 and VPS34 expression …
Immunoblot (A) and qRT-PCR analysis (B) of MAGEA6 variants identified from the ICGC lung cancer database. MAGEA6 variant expression levels are normalized to those of HEK293T cells transfected with …
Key Resources Table.
List of clinical trials of cancer immunotherapy targeting MAGEA antigens.
Clinical trials of MAGEA-targeted immunotherapy reported from clinicaltrials.gov.
MAGEA variants reported in skin, lung and pancreas cancer patients.
Recurrent mutations of MAGEA3, A4, A6, A10 and A12 genes identified from skin, lung and pancreas cancer patients in ICGC, TCGA and COSMIC databases.