The NCBI BLAST server was used to identify proteins that are homologous to SidJ. Sequences corresponding to the kinase-like domain of SidJ (315–756) were aligned by Clustal Omega and colored using Multiple Align Show (http://www.bioinformatics.org/sms/index.html). SidJ residue numbers are marked above the alignment with secondary structural elements drawn above. Identical residues are highlighted in red and similar residues in yellow. Kinase catalytic residues located in the active site are marked with red stars, while residues at the migrated nucleotide-binding pocket are marked with blue stars. Conserved kinase motifs are highlighted as follows: glycine-rich loop (red), VAIK motif (blue), HRD motif (gold), catalytic loop (yellow), DFG motif (brown) and activation loop (green). NCBI accession numbers are as follows: SidJ, Legionella pneumophila, YP_096168.1; SidJ, Legionella longbeachae, WP_003634608.1; SdjA, Legionella pneumophila, YP_096515.1; hypothetical protein A3E83_09250, Gammaproteobacteria bacterium RIFCSPHIGHO2_12_Full_41_20, OGT46295.1; putative uncharacterized protein, Waddlia chondrophila 2032/99, CCB91008.1; hypothetical protein COB53_07685, Elusimicrobia bacterium, PCI37048.1; PBS lyase HEAT domain protein repeat-containing protein (PLHD), Methanosaeta harundinacea KUK97762.1; hypothetical protein, Desulfovibrio hydrothermalis WP_015335088.1; hypothetical protein, Delta proteobacterium NaphS2, WP_006420030.1.