Low FoxO expression in Drosophila somatosensory neurons protects dendrite growth under nutrient restriction
- Weill Institute for Cell and Molecular Biology and Department of Molecular Biology and Genetics, Cornell University, United States
Figures
Figure 1 with 3 supplements
Nutrient restriction affects the growth of epidermal cells and C4da neurons differentially.
(A–B’) Double labeling of ddaC neurons by ppk-CD4-tdTom (A and B) and epidermal cells by the septate junction marker Nrg-GFP (A’ and B’) in the high yeast (HY, 8%) condition at 120 hr after egg …
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Figure 1—source data 1
Larvae growth data for Figure 1 and Figure 1—figure supplement 1, 2, 3.
- https://cdn.elifesciences.org/articles/53351/elife-53351-fig1-data1-v2.xlsx
Figure 1—figure supplement 1
The effects of nutrient restriction on dendrite growth of C4da neurons.
(A–C) Quantification of average terminal dendrite length (A), branch number (B) and terminal branch number (C) of C4da neurons in HY at 120 hr AEL and in LY at 264 hr AEL. n = 16 for HY, n = 14 for …
Figure 1—figure supplement 2
Development of C4da neurons and epidermal cells in high and low yeast.
(A–F’) Double labeling of ddaC neurons by ppk-CD4-tdTom (A–F) and epidermal cells by Nrg-GFP (A’–F’) in HY and LY conditions at times indicated. The red dotted line in (C) indicates how the segment …
Figure 1—figure supplement 3
Growth of the larval body and C4da neurons under starvation.
(A) Quantification of normalized dendrite length before and after starvation. n = 14 for 84 hr AEL in HY; n = 31 for 96 hr AEL in HY; n = 11 for 108 hr AEL in HY; n = 15 for 84 hr AEL + 12 hr …
Figure 2 with 1 supplement
The InR-Tor pathway underlies the preferential neuronal growth under nutrient stress.
(A–F) ddaC neurons in the Gal4ppk control (A and D) and animals expressing Gal4ppk-driven InR RNAi (B and E) and Tor RNAi (C and F) in HY and LY conditions. (G) Quantification of normalized dendrite …
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Figure 2—source data 1
InR-Tor pathway manipulation data for Figure 2 and Figure 2—figure supplement 1.
- https://cdn.elifesciences.org/articles/53351/elife-53351-fig2-data1-v2.xlsx
Figure 2—figure supplement 1
The effects of suppressing InR and Tor in ddaC neurons and epidermal cells in HY and LY conditions.
(A–F) ddaC neurons in the Gal4ppk control (A and D) and animals expressing Gal4ppk-driven InR DN (B and E) and Tor DN (C and F) in HY and LY conditions. (G) Larval body expression patterns of Gal4R16…
Figure 3 with 2 supplements
The lack of autophagy induction protects ddaC neuron growth under nutrient stress.
(A and B) mCherry-Atg8a (magenta) in ddaC soma (green) and epidermal cells in HY and LY conditions. The insets show mCherry-Atg8a at the soma of ddaC (1) and epidermal cells (2). The dotted lines …
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Figure 3—source data 1
Autophagy data for Figure 3 and Figure 3—figure supplements 1, 2.
- https://cdn.elifesciences.org/articles/53351/elife-53351-fig3-data1-v2.xlsx
Figure 3—figure supplement 1
Autophagy flux in C4da neurons and epidermal cells.
(A and B) Lamp-mCherry (magenta) in ddaC soma (green) and epidermal cells in HY and LY conditions. The insets in (A) and (B) show Lamp-mCherry at ddaC soma (1) and epidermal cells (2). All images …
Figure 3—figure supplement 2
Effects of Atg8a knockdown in C4da neurons and Mitf-nGFP expression pattern.
(A–D) ddaC neurons in the Gal4ppk control (A and C) and animals expressing Gal4ppk-driven Atg8a RNAi (B and D) in HY and LY conditions. (A) and (C) are the same as Figure 2A and D, respectively. (E …
Figure 4 with 3 supplements
FoxO is differentially expressed in C4da neurons and epidermal cells to regulate their distinct responses to nutrient stress.
(A and B) FoxO-GFP (green) in da neurons (magenta) and epidermal cells in HY and LY conditions in 2D projections. The insets for (A) and (B) show FoxO-GFP levels at ddaC somas (1) and epidermal …
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Figure 4—source data 1
foxo analyses data for Figure 4 and Figure 4—figure supplements 1, 2, 3.
- https://cdn.elifesciences.org/articles/53351/elife-53351-fig4-data1-v2.xlsx
Figure 4—figure supplement 1
The effects of LOF of Sesn, crc, Sirt2, Rheb, and slif on C4da dendrite growth.
(A) Quantification of epidermal cell size ratio (Gal4R16D01 cells/WT cells) in HY and LY conditions for genotypes indicated. Two-way ANOVA, Posthoc contrasts with a Dunnett correction. The …
Figure 4—figure supplement 2
FoxO expression.
(A and B) FoxO-GFP distribution in epidermal cells under normoxia (A) and hypoxia (B). (C and D) FoxO staining (green) in da neurons (magenta) and epidermal cells in HY and LY conditions in 2D …
Figure 4—figure supplement 3
foxO-Gal4 and effectiveness of foxo RNAi.
(A) Diagram of foxO-Gal4. (B and C) Gal4foxo-driven tdTom expression in optical lobe (B) and VNC (C) in LY condition. (D and E) UAS-FoxO-GFP in Gal4R16D01 control (D) and animals co-expressing foxo …
Figure 5
FoxO exerts its effects through modulating Tor signaling and autophagy.
(A and B) pRpS6 staining (magenta) of ddaC neurons (green) and epidermal cells in Gal4ppk-driven UAS-foxo animals in HY and LY conditions in 2D projections. The insets for (A) and (B) show pRpS6 …
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Figure 5—source data 1
FoxO OE data for Figure 5.
- https://cdn.elifesciences.org/articles/53351/elife-53351-fig5-data1-v2.xlsx
Figure 6
Nutrient stress-induced dendrite overgrowth sensitizes neurons.
(A) A plot showing the percent of responders (respond within 20 s) versus temperature. n = number of larvae; n = 90 for HY and LY at each temperature. (B) A plot showing the percent of fast …
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Figure 6—source data 1
Behavior analyses data for Figure 6.
- https://cdn.elifesciences.org/articles/53351/elife-53351-fig6-data1-v2.xlsx
Figure 7
Different types of somatosensory neurons respond to nutrient stress differentially.
(A and B) C1da neurons in HY and LY conditions. (C) Quantification of ddaE dendrite length in HY and LY conditions. Each circle represents a neuron; n = 20 for HY, n = 14 for LY. student’s t-test. (D…
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Figure 7—source data 1
Other da neuron classes data for Figure 7.
- https://cdn.elifesciences.org/articles/53351/elife-53351-fig7-data1-v2.xlsx
Figure 8
Model of nutrient-regulated da neuron and epidermal cell growth.
See discussion for details. Supplementary file - Key Resources Table.
Additional files
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Supplementary file 1
Key Resources Table.
- https://cdn.elifesciences.org/articles/53351/elife-53351-supp1-v2.xlsx
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Transparent reporting form
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