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Cardiac endothelial cells maintain open chromatin and expression of cardiomyocyte myofibrillar genes

  1. Nora Yucel  Is a corresponding author
  2. Jessie Axsom
  3. Yifan Yang
  4. Li Li
  5. Joshua H Rhoades
  6. Zoltan Arany  Is a corresponding author
  1. Perelman School of Medicine, University of Pennsylvania, United States
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Cite this article as: eLife 2020;9:e55730 doi: 10.7554/eLife.55730

Abstract

Endothelial cells (ECs) are widely heterogenous depending on tissue and vascular localization. Jambusaria et al. recently demonstrated that ECs in various tissues surprisingly possess mRNA signatures of their underlying parenchyma. The mechanism underlying this observation remains unexplained, and could include mRNA contamination during cell isolation, in vivo mRNA paracrine transfer from parenchymal cells to ECs, or cell-autonomous expression of these mRNAs in ECs. Here, we use a combination of bulk RNASeq, single-cell RNASeq datasets, in situ mRNA hybridization, and most importantly ATAC-Seq of FACS-isolated nuclei, to show that cardiac ECs actively express cardiomyocyte myofibril (CMF) genes and have open chromatin at CMF gene promoters. These open chromatin sites are enriched for sites targeted by cardiac transcription factors, and closed upon expansion of ECs in culture. Together, these data demonstrate unambiguously that the expression of CMF genes in ECs is cell-autonomous, and not simply a result of technical contamination or paracrine transfers of mRNAs, and indicate that local cues in the heart in vivo unexpectedly maintain fully open chromatin in ECs at genes previously thought limited to cardiomyocytes.

Introduction

Endothelial cells (ECs) are the most abundant non-blood cells in the body, and form the inner layer of all vessels in all organs. ECs carry out a wide range of critical functions, including providing a barrier between blood and the underlying parenchyma, regulating transport of nutrients and waste across that barrier, regulation of immune cell extravasation, maintaining intravascular hemostatic homeostasis, and controlling blood flow and systemic vascular resistance. These numerous roles differ widely depending on anatomical site, developmental stage, and physiological state. Consistent with this remarkable variability of function, extensive transcriptional and functional heterogeneity of ECs across tissue types, vascular localization (e.g. arterial, venous, or lymphatic), and developmental stage has been characterized in a number of studies. However, the chromatin landscape underlying these differences remains poorly understood.

Interestingly, numerous investigators have noted anecdotally, in discussions at various meetings, that cardiac ECs contain mRNAs that encode various myofibrillar proteins normally expressed in cardiomyocytes, including myosins, troponins, and titin. Most recently, Jambusaria et al. collated EC-specific RNAseq datasets from heart, brain, and lung, to formally show that ECs in these three tissues contain mRNAs normally ascribed to their respective underlying parenchyma (Jambusaria et al., 2020). This observation has been variably ascribed to technical artifacts during RNA preparations (i.e. contamination by RNAs from cardiomyocytes) or to presumed transfer in vivo of RNAs from cardiomyocytes to ECs via, for example, exosome transfer (although this has never been demonstrated), but these conclusions have been controversial. An alternative hypothesis is that cardiac ECs may in fact express these RNAs intrinsically. In this context, it is relevant to note that cardiac ECs originate from tri-potential precursor cells that differentiate to cardiomyocytes, smooth muscle cells, and ECs. It may therefore be that cardiac ECs maintain epigenetic memory of a cardiomyocyte-like precursor.

To distinguish these possibilities, we use here single-cell RNAseq as well as bulk ATACSeq to characterize the chromatin accessibility signature of cardiac ECs in vivo. We show that cardiac ECs in fact maintain chromatin accessibility and transcriptional activation of myofibrillar genes typical of underlying cardiomyocyte parenchyma, but not of the immediately surrounding stromal cells. The presence of these mRNAs in cardiac ECs is thus a direct consequence of their expression in ECs.

Results

Cardiac ECs express myofibrillar genes normally associated with cardiomyocytes

We first sought to define the complete signature of actively translated mRNAs in cardiac ECs. To do so, we leveraged the NuTRAP mouse model, in which ribosomes are genetically tagged with GFP, conditional on Cre expression (Roh et al., 2017). EC-specific Cdh5-Cre NuTRAP mice thus allow for immuno-precipitation of ribosome-bound, and thus actively translated, RNA strictly from ECs, without the need for cell-isolation. RNASeq from NuTRAP-tagged cardiac ECs showed, as expected, enrichment of known EC-specific genes. Surprisingly, however, we also found significant contribution of mRNAs from cardiomyocyte myofibril (CMF) genes which, although depleted compared to the non-endothelial fraction, were still amongst the highest expressed genes in cardiac ECs (Figure 1A). To begin to ascertain whether this contribution was due to technical contamination, that is pulldown of mRNAs originating from cardiomyocytes instead of ECs, we performed Kendall-Tau rank-order analysis. As did Jambusaria et al, we reasoned that contamination would reflect the relative expression levels of CMF genes from the whole heart (Figure 1A–B). Endothelial and non-endothelial heart expression of highly expressed genes were only poorly correlated (0.216), suggesting that the presence of CMF mRNAs is not solely due to contamination.

Cardiac endothelial cells express myofibrillar genes normally associated with cardiomyocytes.

(A) RNASeq of Translating Ribosome Affinity Purification (TRAP) endothelial RNA freshly isolated from hearts of Cdh5-NuTRAP animals. Expression (Log2CPM) of immunoprecipitated endothelial RNA (IP) versus non-endothelial RNA from the remaining fraction after IP (supernatant). Shown in the blue box are the top 300 expressed genes, with CMF genes highlighted in red. Kendall-Tau rank analyses performed on these top 300 expressed genes. (B) Relative expression of selected genes from freshly isolated tissue-specific adult endothelial cell TRAP, collected from (Rpl22fl/fl, Tek2-Cre+)/animals (Cleuren, et al, GSE138630) vs total tissue TRAP RNA (Rpl22fl/fl, EIIa-Cre+/0). (C) Kendall-Tau analysis of TRAP vs total tissue of heart tissue from Cleuren, et al. Analyses performed on the highest 300 expressed genes in the total heart tissue (D) Multidimensional scaling (MDS) clustering of the top 1000 variably expressed genes in the tissues analyzed in (B). (E) Relative expression of the indicated genes in tissue-specific adult murine endothelial cells in data sets from Coppiello et al., 2015 (GSE48209) and Nolan et al., 2013 (GSE47067) (F) Relative expression of tissue-specific fetal human endothelial RNASeq from Marcu et al., 2018 (GSE114607).

To determine if the expression of CMF genes is enriched in ECs from the heart compared to other tissues, and to determine if other investigators had also detected CMF gene expression in heart ECs, we gathered expression data from published gene expression omnibus. We focused on datasets that used prospective isolation of ECs by flow cytometry using either genetic or surface markers in order to minimize contamination by the underlying parenchyma. All data analyzed was from uncultured, freshly isolated ECs or endothelial material. Three mouse studies (Cleuren et al., 2019; Coppiello et al., 2015; Nolan et al., 2013) and one study using human fetal tissue (Marcu et al., 2018) were identified. We first focused on the Cleuren, et al dataset, which, akin to the NuTRAP system we used above, used the Rpl22fl/fl Tek-Cre+/0 mice to characterize the endothelial translatome in various tissues. Similar to our own analyses, as well those published by Jambusaria et al, we found significant expression of CMF genes in the EC translatome in this dataset (Figure 1B). Moreover, the expression of these genes was limited to heart ECs, and not seen in translatomes from ECs in other organs. Kendall Tau rank analysis of the expression of the top 300 highest expressed genes (which included CMF genes) in ECs vs the total heart translatome again showed low correlation (0.05–0.28, Figure 1C), casting doubts on the notion that CMF mRNAs in ECs originate from contaminating cardiomyocyte-derived mRNAs. To take an unbiased approach to this question, we clustered by principle component analysis the translatome RNAseq datasets, comparing various whole tissues to their respective ECs (Figure 1D). Multidimensional scaling of these data showed that ECs surprisingly cluster closer to their tissue of origin than to other ECs, and this was particularly true in the heart.

The next three datasets used FACS-isolated ECs to separate EC mRNAs, rather than immunoprecipitated EC-specific translatomes. Despite the different approach, analyses of these datasets again showed an enrichment of CMF gene expression in cardiac ECs, but not in ECs from other organs (Figure 1E–F). In contrast, expression of known cardiomyocyte-specific transcription factors, as well as metabolic genes highly enriched in cardiomyocytes, were not significantly different in cardiac ECs compared to other ECs. Together, analyses of these datasets demonstrate that CMF gene expression in ECs: (1) is unique to cardiac ECs; (2) is generally limited to CMF genes and not to other genes highly expressed in cardiomyocytes such as mitochondrial genes; and (3) has been detected by numerous previous studies, including most recently Jambusaria et al.; and (4) is unlikely to be caused solely by contamination.

Expression of at least one CMF gene is seen in ~60% of cardiac ECs

We next wanted to determine if CMF gene expression in ECs is ubiquitous to all cardiac ECs, or is limited to a subset of ECs. In addition, we wanted to determine whether CMF gene expression is also present in other resident cell types in the heart. To do so, we leveraged Tabula Muris single-cell RNAseq data (Tabula Muris Consortium et al., 2018), and compared CMF gene expression in cardiac ECs and fibroblasts. We first used the single-cell analysis package Seurat v3 (Stuart et al., 2019) to identify marker genes characteristic of cardiomyocytes, ECs or fibroblasts, that is signatures for each cell type (Supplementary file 1). In the cardiomyocyte signature, we selected 15 genes that were expressed in >60% of cardiomyocytes, and whose expression was at least 10-fold higher than that in non-cardiomyocytes cells. Expression of each of these 15 genes was then evaluated in single cardiac and lung ECs and fibroblasts. The number of cardiac ECs identified as expressing each of these cardiomyocyte signature genes was dramatically higher than lung ECs or fibroblasts, and 2–4 fold higher than heart fibroblasts (Figure 2A). A total of 59.3% of all cardiac ECs expressed at least one of the 15 CMF genes, 1.75-fold more often than cardiac fibroblasts, and about five times more often than lung ECs (which were driven primarily by one gene, Myl4; Figure 2A–B). The majority of CMF-expressing ECs only showed expression of one or two genes (Figure 2B). This distribution, as opposed to one subpopulation of cells expressing all CMF genes, suggests that expression is not being driven by cardiomyocytes misclassified as ECs, or by EC/cardiomyocyte doublets, again arguing against contamination.

Expression of at least one CMF gene is seen in ~59% of cardiac ECs.

(A): Analysis of mouse heart and lung single cell data from Tabula Muris in Schaum, et al (Nature, 2018). Fifteen cardiac myofibrillar (CMF) genes were chosen based on high, unique expression in the cardiomyocyte cell subsets. Positive expression was determined as a normalized count (ln(1+Counts-per-million)) greater than or equal to one. (B): Percentage of cardiomyocytes vs endothelial cells or fibroblasts within the heart and lung that express 1–15 of the selected CMF genes. (C–E). Analysis of unspliced vs spliced transcripts in Tabula Muris data. Data shown are merged counts for all cells within the cardiomyocyte (CM), endothelial cell (EC), or fibroblast (FB) cell subsets, as annotated in Tabula Muris. Shown are CMF genes with a unspliced percentage >= 1% in cardiomyocytes. Marker genes for cell-subsets are shown in Supplementary file 1, and unspliced/total ratios can be found in Supplementary file 2 (C) Overall expression shown as log2(1+CPM) for selected CMF genes (D) Unspliced/total fraction for selected CMF genes in cardiomyocyte, endothelial cells or fibroblast cell subsets within the heart. (E) Genome track for aligned Tabula Muris RNA-Seq data for cardiomyocyte, cardiac EC, lung EC or cardiac fibroblast populations. In upper panel is the full track for CMF gene Myom2, with inset showing a regions with intronic reads specific to cardiac ECs.

Finally, to test in yet another way for evidence of contamination, we looked for the presence of immature, pre-spliced mRNAs of cardiac genes in ECs. We reasoned that contaminating RNAs from cardiomyocytes would originate largely from the cytosol, and therefore be primarily mature, spliced RNA. Velocyto (La Manno et al., 2018) was used to quantify spliced and unspliced gene counts for cardiomyocyte, EC, and fibroblast cell subsets as annotated in Tabula Muris. Within each subset, cells were merged, and the ratio of unspliced mRNAs/total mRNAs was calculated for each population (Supplementary file 2). We focused on cardiomyocyte genes with detectable unspliced mRNAs (>1%; a total of 53 genes). In this gene set, the median spliced percentages for cardiac ECs and fibroblasts cells were 5.4% and 4.2%, respectively, markedly higher than in cardiomyocytes (2.4%; Figure 2C). An example of this is shown in the RNA-seq track for Myom2 in Figure 2D, illustrating: (1) significant expression of these genes in cardiac ECs, but not in lung ECs and (2) importantly, significant presence of unspliced introns in ECs, but not in cardiomyocytes.

Overall, these data demonstrate: (1) that EC expression of CMF genes is limited to cardiac ECs; (2) that cardiac fibroblast cells also express these genes but much less frequently than cardiac ECs; (3) that at any given time only a subset of cardiac ECs express only a subset of CMF genes, suggesting stochastic expression; and (4) that cardiac ECs express both spliced and unspliced RNAs from CMF genes, the latter at a higher frequency than cardiomyocytes, indicating cell-intrinsic expression.

CMF mRNAs are detectable in EC nuclei ex vivo and in situ in vivo

The results above strongly suggest, but fall shy of formally proving, that the presence of CMF mRNAs in cardiac ECs is not caused by contamination from cell-free RNA derived from cardiomyocytes, whose cytoplasmic volume is much larger than fibroblasts or ECs. To address this possibility in yet another way, we isolated EC nuclei, rather than whole cells, from mouse hearts, by fluorescent sorting from Cdh5-Cre/NuTRAP animals. To confirm specificity of the sorting strategy, nuclei were co-stained with PCM-1, known to specifically mark cardiomyocyte nuclei (Bergmann et al., 2011), which revealed no overlap between GFP+ (EC) and PCM-1+ (cardiomyocyte) nuclei populations (Figure 3A). Doublets were eliminated by FSC-A/FSC-H and as well as DAPI gating (2 n) (Figure 3—figure supplement 1). Furthermore, the observed ~20% GFP+ (EC) nuclei and ~30% PCM-1+ (cardiomyocyte) nuclei matched previously estimated reports of cardiac cell content (Hu et al., 2018; Pinto et al., 2016). Quantitative PCR with RNA from GFP+ (EC) nuclei showed strong enrichment (~5-fold) of EC-specific transcripts (Pecam1, Gpihbp1), and depletion (10–15 fold) of fibroblast/pericyte-specific transcripts (Dpt, Cspg4, Pdgfrb). In contrast, CMF genes were depleted to a much lesser extent (2–3 fold; Tnnt2, Myh6, Myh7, Actc1) (Figure 3B,C). Hence, CMF gene expression persists in nuclei isolated from cardiac ECs.

Figure 3 with 2 supplements see all
CMF mRNAs are detectable in EC nuclei ex vivo and in situ in vivo.

(A) Representative flow cytometry plot of GFP expression from isolated nuclei from Cdh5/NuTRAP mice. About 20% of nuclei are GFP+ (endothelial), with no co-staining with the cardiomyocyte-specific nuclear marker PCM1. Gating scheme for identification of single nuclei shown in Figure 3—figure supplement 1 (B) Relative expression (by qPCR) of nuclear RNA from isolated GFP+ (endothelial) or GFP- (non-endothelial) nuclei. Blue box: stromal genes; red box: CMF genes. (C) Average fold change of expression (GFP+/GFP-) in (B) for fibroblast or CMF genes. (D) Confocal images of heart sections. RNAScope probes for Cdh5 (endothelial) and CMF (Tnnt2) mRNA were co-stained with Pecam1 antibody. Shown are confocal slices of 0.96 μm thickness. Additional images are available Figure 3—figure supplement 2.

Tissue dissociation and nuclear fluorescent sorting could, in principle, aberrantly activate expression of CMF genes in EC nuclei. To rule out this potential artifact of nuclear isolation, we used RNAscope to directly visualize CMF RNA within cardiac EC nuclei in situ in intact hearts. Using confocal imaging, we found co-localization of Tnnt2 RNA within ECs in the heart, as identified by Pecam1 (CD31) antibody staining (Figure 3DFigure 3—figure supplement 2A–B). Quantification of nuclei with co-localization of both Cdh5 and Tnnt2 RNA showed presence of Tnnt2 in ~12% of endothelial (Cdh5+) cell nuclei (Figure 3—figure supplement 2C–D), a proportion that is similar to the number of ECs in Tabula Muris that are Tnnt2 positive (Figure 2A). Altogether these results unambiguously show that expression of CMF genes in ECs is not due to contamination from parenchymal cells, and strongly suggest that expression of CMF genes stems from direct expression from EC chromatin.

Cardiac ECs maintain open chromatin at CMF genes

The presence of CMF transcripts in ECs, if not technical contamination, could potentially reflect a biologic paracrine contribution of cardiomyocyte-derived RNAs transmitted to ECs via, for example, exosomes or microvesicles, as previously described in non-cardiac contexts (Skog et al., 2008; Valadi et al., 2007). On the other hand, our single-cell analysis, RNAScope and nuclear sorting data strongly suggested EC-intrinsic transcription of CMF genes. To distinguish these possibilities, we used ATAC-Seq to determine directly whether cardiac ECs have open chromatin at CMF genes, reasoning that if there is EC-intrinsic expression, there should also be some detectable open chromatin at cardiomyocyte-specific genes. We performed genome-wide ATAC-Seq (Buenrostro et al., 2015) on nuclei sorted from Cdh5-Cre/NuTRAP animals, and compared GFP positive (endothelial) and GFP negative (non-endothelial) populations (Figure 4A–C). We then quantified average enrichment at the transcriptional start sites (TSSs) of endothelial, fibroblast, and cardiomyocyte marker gene sets (Figure 4B), as well as per-gene comparison of read density at the TSSs of GFP+ (EC) vs GFP- (non-EC) samples (Figure 4C). As expected, EC nuclei had an open chromatin signature at EC-specific genes (Figure 4A: Erg, Cdh5, Gphibp1, Egfl7; Figure 4B green line; and Figure 4C, green circles), and markedly reduced open chromatin signature at fibroblast genes (Figure 4A: Dpt, Cspg4, Pdgfrb, Col6a2; Figure 4B blue line; and Figure 4C blue triangles). Strikingly, however, the chromatin of CMF genes was quantitatively as open in ECs as in cardiomyocyte nuclei (Figure 4A: Tnni3, Tnnt2, Tnnc1, Myh6; Figure 4B red line; and Figure 4C, red diamonds). In addition to accessibility at promoter-TSS regions, most peaks associated with enhancer regions of cardiomyocyte marker genes (intergenic and intronic) were also seen in cardiac ECs (Figure 4—figure supplement 1A–B) We did identify a small number of enhancer regions that were unique to the non-EC nuclei, including an enhancer region upstream of the gene encoding the cardiac transcription factor Nkx2-5, and an intronic enhancer within the Dmd gene (Figure 4—figure supplement 1C). These data demonstrate that cardiac ECs have open chromatin at CMF genes, and exhibit quantitatively similar accessibility at these genes at both TSS and enhancer regions as the underlying parenchyma.

Figure 4 with 1 supplement see all
Cardiac ECs maintain open chromatin at CMF genes.

(A) Representative ATACSeq gene tracks for cardiomyocyte, fibroblast, or endothelial cell genes in isolated GFP+ (endothelial, in red) or GFP- (non-endothelial, in black) nuclei. (B) Genome-wide open chromatin at the transcriptional start sites (TSSs) of cardiomyocyte, stromal, or endothelial cell genes in GFP- (non-endothelial) and GFP+ (endothelial) nuclei. Note chromatin of cardiomyocyte genes (red) is as open in ECs (GFP+) as non-ECs (GFP-). (C) Comparison of accessibility in GFP- vs GFP+ nuclei at TSS peaks (+/- 250 bp) for EC genes (green), cardiomyocyte genes (red), and stromal genes (blue). Note again that chromatin of cardiomyocyte genes is as open in EC as non-EC nuclei. (D) Motif enrichment analysis of ATACSeq peaks unique to non-endothelial (GFP-) nuclei (top), unique to endothelial (GFP+) nuclei (bottom), and shared peaks (middle). Right panels: gene ontology (GO) analysis of genes within 2 kb of each peak set. Full statistics and GO annotations for peak regions shown in Supplementary file 3. Additional analyses shown in Figure 4—figure supplement 1.

HOMER analysis (Heinz et al., 2010) revealed that ATAC-seq peaks unique to GFP+ (EC) nuclei (Supplementary file 3) were enriched for known endothelial transcription factor binding sites (ERG, ETS), consistent with their endothelial identity. Genes within 2 kb of GFP+ unique peaks showed GO enrichment for vascular development-associated terms (Figure 4D, lower panel). In parallel, peaks unique to GFP- (non-EC) nuclei were enriched for the smooth muscle and cardiac fibroblast transcription factor TCF21, as well as genes associated with neurogenesis and extracellular matrix organization (upper panel). Most interestingly, however, peaks that overlapped between GFP- and GFP+ nuclei (middle panel) were significantly associated with genes of cardiomyocyte development and contraction, and HOMER analysis showed enrichment for the cardiac transcription factors GATA4 and MEF2. These unbiased analyses thus strongly support genome-wide open chromatin at CMF genes in both ECs and cardiomyocytes.

Finally, we compared these epigenetic data with heart EC-specific genes (as opposed to ECs from other organs) identified in previous studies. We focused on genes with >2 fold increased expression compared to brain or lung ECs in the data from Cleuren et al., and a baseline expression of at least 10 transcripts per million (TPM) in both that data set and ours (Figure 5A). Interestingly, not all of these cardiac EC-enriched genes revealed open chromatin (RPKM <1.5 at the TSS), suggesting that these transcripts may in fact have origins outside the ECs. GO analysis of the genes with closed chromatin revealed largely fibroblast-like annotations (Figure 5B, left). In contrast, GO analysis of the genes with open chromatin (RPKM >3 at the TSS) once again revealed strong enrichment for CMF genes, as well as metabolic genes related to handling of fatty acids, the primary fuel of cardiomyocytes (Figure 5B, right).

Not all genes expressed in endothelial cells (ECs) have open chromatin.

(A) Gene expression (data from Cleuren et al.; GSE138630) and gene accessibility of cardiac EC-enriched genes (with expression of at least 10 CPM, and twofold or higher expression compared with brain or lung endothelial cells) in cardiac endothelial (GFP+) nuclei. Blue: relatively close chromatin (Log2RPKM +/- 250 bp at TSS peaks <3); red: relatively open chromatin (>3). (B) Representative tracks and gene ontology (GO) analysis of cardiac EC-enriched genes with closed (blue) or open (red) chromatin.

In summary, these data demonstrate that, while as expected cardiac ECs have open chromatin at EC-specific genes and closed chromatin at fibroblast cell-specific genes, they broadly share open chromatin regions with the cardiomyocytes that make up the underlying parenchyma.

Open chromatin and expression of CMF genes in ECs requires in vivo cues

Finally, to determine whether open chromatin at CMF is an indelible property of cardiac ECs, or whether it requires active maintenance, we isolated primary cardiac or lung ECs and assayed open chromatin by ATAC-qPCR and ATAC-Seq after expansion in culture, that is in the absence of local cues provided in vivo (Figure 6A). Chromatin at the EC-specific genes Erg and Cdh5 remained open during this time, consistent with maintenance of endothelial identity (Figure 6B). In sharp contrast, chromatin at CMF genes (Tnnt2, Myh6) completely closed within this time span, and had similar accessibility to freshly isolated lung endothelial nuclei (Figure 6C). Interestingly, accessibility at Pdgfrb, a pericyte/fibroblast marker, was increased in culture, although Cspg4 and Tcf21 remained closed (Figure 6D), likely reflecting some endothelial-to-mesenchymal transition occurring under culture conditions. Similarly, ATAC-Seq of cultured cardiac ECs showed that accessibility at CMF genes was overall reduced compared to endothelial or fibroblast genes (Figure 6E). Annotation of ATACSeq peaks (Figure 6F; Supplementary file 4) showed an enrichment for in genes involved with metabolic processes, signal transduction, and cell differentiation in newly opened peaks. In contrast, peaks that closed in culture were primarily associated with CMF terms, including heart development and muscle contraction. We conclude that open chromatin at CMF genes in cardiac ECs in vivo requires active maintenance by the surrounding parenchyma.

Open chromatin and expression of CMF genes in ECs requires in vivo cues.

(A) Experimental scheme. N = 2–4 biological replicates. ATAC-qPCR and ATAC-Seq was performed on freshly isolated endothelial nuclei, or cultured endothelial cells from heart or lung. qPCR regions were identified based on peaks identified in sequencing data. (B) Relative accessibility (compared to housekeeping genes, Rpsa and Tbp of endothelial cell genes, Cdh5 and Erg). (C) Relative accessibility of cardiomyocyte genes (Tnnt2, Myh6). (D) Relative accessibility of fibroblast-signature genes (Pdgfrb, Cspg4, Tcf21) (E) Representative TSS enrichment plot of cultured cardiac endothelial cells. Enrichment shown for cardiomyocyte-specific, endothelial or fibroblast specific gene sets (gene sets previously identified in Supplementary file 1). (F) GO enrichment for peaks that open (left) or close (right) in cultured cardiac endothelial cells vs freshly isolated. Complete statistical analyses by DESEQ2 and GO annotations shown in Supplementary file 4.

Discussion

Overall, we show here that cardiac ECs possess transcriptional and epigenetic signatures of cardiomyocytes, but not other cell types within the heart. Moreover, the presence of open chromatin at cardiomyocyte-specific genes, as well as the nuclear expression of these genes in ECs, demonstrates significant EC-intrinsic transcriptional contribution of these mRNAs. A gene expression signature in ECs that mirrors the underlying parenchyma is also apparent in public datasets from other studies, and was recently reported in the literature for the first time by Jambusaria et al. These studies, however, left open the question of the mechanism by which these mRNAs were to be found in cardiac ECs. In numerous unofficial discussions at scientific meetings, the observation has in general been ascribed to either technical contamination, or to cardiomyocyte-derived mRNA transferring to ECs via extracellular vesicles or other paracrine mechanism. Our results, in contrast, demonstrate that these CMF mRNAs originate in ECs themselves, via active maintenance of open chromatin and transcription. It must be noted that the functional consequence of this shared signature, and whether it results in translation of CMF transcripts, remains to be determined.

The notion that ECs actively express CMF genes has been contentious. However, at this point, the arguments against contamination as an explanation for the presence of CMF mRNAs in cardiac ECs are numerous and compelling:

  1. Kendall Tau correlations demonstrate lack of correlation between genes highly expressed in cardiomyocytes and those potentially contaminating ECs. Were contamination to be occurring, these levels of expression would correlate.

  2. In the single cell RNAseq datasets, only a subset of ECs contained only a subset of CMF genes (most often only one). If CMF mRNAs were being captured by ECs by contamination, this would be observed in all cells. Even in the unlikely scenario that only a subset of ECs captured CMF mRNAs, they would capture all CMF genes instead of on average only one.

  3. CMF mRNAs in ECs are relatively more unspliced than in cardiomyocytes, supporting the presence of nascent mRNAs in ECs.

  4. Nuclear qPCR reveals the same observations as whole-cell RNAseq. If contamination were occurring in this setting, it would require the formation of nuclear doublets, which was ruled out by forward/side scatter, and by counterstaining with PCM-1, a marker specific for cardiomyocyte nuclei.

  5. RNAscope studies demonstrate direct visualization of CMF transcripts in EC nuclei in situ, ruling out artifacts of cell preparation or sorting during the RNAseq studies.

  6. ATACseq on chromatin of cardiac ECs demonstrates fully open chromatin at CMF genes. Unlike highly variable mRNA abundance, chromatin abundance is proportional to cell count. Thus, if contamination were occurring in this setting, EC chromatin would be, on average, only mildly open (proportional to the level of contamination), because the majority of the signal would still stem from ECs.

  7. In aggregate, these data provide unambiguous support for the notion that cardiac ECs have open chromatin at CMF genes, and actively transcribe these genes. However, these data do not indicate whether CMF genes are also translated, and serve similar functions in ECs as they do in cardiomyocytes.

Interestingly, we find that the expression of CMF genes, including immature, unspliced transcripts, in ECs is detectable in only a subset of cells (~60%), and that in general only 1–2 genes are detectable, and not always the same ones. In contrast, the chromatin at these genes, although evaluated only in bulk, appears to be as open as that of cardiomyocytes. These observations suggest that chromatin is maintained open at these genes in all cardiac ECs, but that rates of transcription are relatively low and stochastic, and that there may not be a defined subset of ECs that express CMF genes. We hypothesize that this fully open chromatin at CMF genes in cardiac ECs may reflect shared developmental origin between cardiomyocytes and ECs. During development, cardiac progenitors that express Nkx2-5 (primary heart field) or Isl1 (secondary heart field) give rise to both ECs and cardiomyocytes (Jia et al., 2018; Moretti et al., 2006; Wu et al., 2006). Thus, accessibility of chromatin at cardiomyocyte-specific genes may be due to epigenetic memory of this shared developmental origin.

An outstanding question remains as to the role of the cardiomyocyte epigenetic and transcriptional signature on endothelial function. Recent studies point towards a potential role in EC maturation. Both developmental and adult cardiomyocyte and ECs share expression of the transcription factor MEF2C, the number one predicted binding site in our analysis of open chromatin peaks shared between cardiac ECs and cardiomyocytes. Endothelial-specific MEF2C has been shown to regulate angiogenesis (Sacilotto et al., 2016), cell integrity and survival (Potthoff and Olson, 2007), as well as response to inflammation (Xu et al., 2015). GATA4, whose motif is highly enriched between cardiac ECs and cardiomyocytes, is expressed by cardiac progenitors and in adult cardiomyocytes, but almost undetectable in ECs. Nonetheless, a recent study Maliken et al., 2018 demonstrated that inducible knockdown of GATA4 in adult ECs resulted in a less mature, PECAM1-‘low’ EC phenotype, including hyperproliferation, reduced differentiation, and impaired tube formation capacity. Although not highlighted in the main figures, the supplementary data from this study reveals that expression of CMF genes, including Myh6, Myl7, Tnnt2 and Sln, are significantly downregulated (25–1.5 fold) upon Gata4 knockdown. These studies, which show that loss of cardiomyocyte transcription factors results in EC ‘immaturity’, are consistent with our observation that cardiomyocyte-specific gene accessibility is lost in cardiac ECs upon culture. We hypothesize that perhaps cardiomyocyte transcription factors, such as GATA4 and MEF2, are responsible for maintaining the open chromatin signature of CMF genes within ECs in the heart, and that CMF gene expression plays a role in cardiac EC maturity.

In summary, we demonstrate here that cardiac ECs maintain open chromatin and active transcription at a number of myofibrillar genes thought to be uniquely expressed in cardiomyocytes. This shared chromatin accessibility landscape is likely maintained by paracrine cues in vivo, and likely serves to maintain the unique phenotype of cardiac ECs.

Materials and methods

Key resources table
Reagent type (species) or resourceDesignationSource or referenceIdentifiersAdditional
information
Strain, strain background (Mus musculus)B6;129S6-Gt(ROSA)26Sortm2(CAG-NuTRAP)Evdr/JCell Rep. 2017 Jan 24; 18(4): 1048–1061.NuTRAPhttps://www.jax.org/strain/029899
Strain, strain background (Mus musculus)B6;129-Tg(Cdh5-cre)1Spe/JJAX strain 017968Cdh5-Cre, VE-Cadherin-CRE
AntibodyPecam1 antibody; Rat monclonalBD PharmigenCat# 558736(1:500)
AntibodyPcm1; Rabbit polyclonalSigmaCat# HPA023370(1:250)
Software, algorithmfastpChen et al., 2018
Software, algorithmBowtie2Langmead and Salzberg, 2012
Software, algorithmGenrichhttps://github.com/jsh58/Genrich
Software, algorithmPicard Toolshttp://broadinstitute.github.io/picard/
Software, algorithmsamtoolsLi et al., 2009
Software, algorithmSTARDobin, 2013
Software, algorithmSeuratStuart et al., 2019
Software, algorithmRhttps://www.r-project.org/
Software, algorithmDiffBindRoss-Innes et al., 2012
Software, algorithmDeeptoolsRamírez et al., 2016
Software, algorithmHomerHeinz et al., 2010
Software, algorithmVelocytoLa Manno et al., 2018
OtherDAPI stainMolecular Probes(1:1000)
OtherRNAscope Probe- Mm-Tnnt2-C3ACD BioCat# 418681-C3
OtherRNAscope Probe- Mm-Cdh5-C2ACDBioCat#
312531-C2
Commercial assay or kitRNAscope Multiplex Fluorescent v2 AssayACD BioCat# 323136
Commercial assay or kitIllumina Tagment DNA Enzyme and BufferIlluminaCat#:200341971.25 uL of enzyme used per 50,000 nuclei for transposition

Nuclei isolation

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Nuclei were isolated from whole heart tissue following the method outlined in Roh et al., 2017. In brief, whole hearts were dounced in 10 mL nuclear preparation buffer (NPB; 10 mM HEPES (pH7.5, 1.5 mM MgCl, 10 mM KCl, 250 mM Sucrose, 0.1% IGEPAL-630/NP-40, 0.2 mM DTT, cOmplete mini EDTA-free protease inhibitor Roche #11836170001)). Homogenates were filtered through 40 µM cell strainers, and spun at 500G for 5 min. Nuclear pellets were washed 1X in NPB buffer, and then resuspended in 2 mL nuclear sort buffer (NSB; 10 mM Tris pH 7.5, 40 mM NaCl, 90 mM KCl, 2 mM EDTA, 0.5 mM EGTA, 0.1% IGEPAL-630/NP-40, 0.2 mM DTT, cOmplete mini EDTA-free protease inhibitor) and passed through a filter-top FACS tube.

TRAP RNA isolation

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Translating Ribosome Affinity Purification (TRAP) was performed as described in Roh, et al. In brief, ~50 mg of fresh heart tissue was dounce homogenized in 6 mL of homogenization buffer (50 mM Tris pH7.5, 12 mM MGCl,, 100 mM KCl, 1% IGEPAL-630/NP-40, 100 µg/mL cycloheximide, 1 mg/mL sodium heparin, 2 mM DTT, 0.2units/µL RNAse inhibitor, 1X cOmplate EDTA-free protease Inhibitor). Samples were centrifuged at 13,000 RPM for 10 min to remove cell debris. The supernatant was then collected and incubated with anti-GFP antibody (5 µg/mL, Abcam ab290) for 1 hr at 4°C. Protein G dynabeads were washed twice in low-salt wash buffer (50 mM Tris, pH 7.5; 12 mM MgCl2; 100 mM KCl; 1% NP-40; 100 μg/ml cycloheximide; 2 mM DTT), added to the homogenates with antibody, and subsequently incubated for 30 min. Dynabeads with immunoprecipitates were washed three times in high-salt wash buffer (50 mM Tris, pH 7.5; 12 mM MgCl2; 300 mM KCl; 1% NP-40; 100 μg/ml cycloheximide; 2 mM DTT). Following the last wash, RLT buffer with β-mercaptoethanol was added to dynabeads, and RNA was extracted using Qiagen Micro RNeasy kit according to the manufacturer’s instructions. For input or supernatant RNA, 5% of total homogenates or IP supernatants unbound to beads (respectively) were mixed with TRIzol and processed according to the manufacturer’s instructions to extract total RNA. All RNA was further cleaned using AMPure bead purification to remove residual salts, and RNA integrity was analyzed by Agilent Bioanalyzer.

Flow cytometry and sorting

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EC nuclei were isolated by florescence activated cell sorting (FACs) on a BD FACS Aria II. Single, non-dividing nuclei were identified by selecting on DAPI for 2 n, followed by FSC-A/FSC-H for doublet removal, and FSC/SSC. From this subset, GFP+ nuclei were selected. GFP positive and negative nuclei were sorted directly into ATAC-Seq lysis buffer (Buenrostro, et al; 10 mM TrisCl, 10 mM NaCl, 3 mM MgCl, 0.1% IGEPAl-630/NP-40) in eppendorf tubes for further processing.

ATAC-Seq

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Sorted nuclei were spun at 500G at 4C for 10 min. ATACSeq reactions were prepared from nuclei according to Buenrostro, et al, using Illumina TDE1 tagment DNA Enzyme and TD buffer (#15027865, 15027866). Libraries were amplified for a total of 13–17 cycles following transposition. Libraries were cleaned to remove very large (>1000) or very small (<50 bp) DNA sequences using AMPure bead cleanup. Library quality was assessed by Bioanalyzer.

RNA-Seq

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For RNASeq, ~25 ng of total RNA was using the NuGEN Ovation V2 kit for cDNA amplification, followed by NEBNext Ultra DNA Library Prep Kit for Illumina for library construction, according to manufacturer’s instructions. A Covaris E220 was used to sonicate cDNA to ~200 bp fragments prior to library construction. Library quality was assessed by Agilent Bioanalyzer. Using a NextSeq 550 sequencer, 75 bp single-end sequencing was performed. Libraries were aligned and quantified using STAR v2.7.0 (Dobin et al., 2013). The mouse reference genome was generated using GRCm38, and gene counts quantified using annotations from GRCm38.99. Parameters used were --runThreadN 8; --genomeDir GRCm38; --sjdbGTFFile GRCm38.99; --sjdbOverHang 100; --outSAMtype BAM SortedByCoordinate; --quantMode GeneCounts.

ATACSeq

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ATACSeq libraries were sequenced on a NextSeq 550 using 35 × 2 reads. Raw data was cleaned and trimmed using fastp (Chen et al., 2018), and mapped to the mm10 mouse genome using bowtie2, v 2.3.4 (Langmead and Salzberg, 2012). Maximum insert size was set to 2000 (-X 2000). Mapped reads were converted to bam and filtered for quality using samtools (Li et al., 2009), with the following parameters: samtools view -h -F 4 -q 10 -bS. Duplicates were removed with Picard (http://broadinstitute.github.io/picard/), using MarkDuplicates (-REMOVE_DUPLICATES True). Libraries were then downsampled to 50 million reads, and Genrich (https://github.com/jsh58/Genrich) was used to call peaks on non-mitochondrial reads using ATAC-seq mode. For peak calling, files were sorted by name using samtools, and blacklist regions were derived from ENCODE. Both replicates were used for consenses peak claling. Parameters for Genrich used were -j -v -y -r -e chrM,chrY -E $BLACKLIST -k OUTFILE.bedgraphish -t REPLICATE1.BAM,REPLICATE2.BAM -o OUTFILE.narrowPeak. For genome browser visualization, bigwig files (.bw) were generated by text parsing to convert. bedgraphish outputs to. bedgraph, and converted to bigwig format bedGraphtoBigWig (http://genome.ucsc.edu/).

Finally, differential peak analysis was performed using Diffbind (Ross-Innes et al., 2012) with peaks generated from Genrich. Deeptools (Ramírez et al., 2016) was used to generate heatmap and profile plots, and Homer (Heinz et al., 2010) for transcription factor motif analysis and peak annotation.

Single-cell data analysis

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In the mouse, in order to identify gene sets representative of endothelial, cardiomyocyte, or fibroblast cells within the heart, gene counts from SMART-Seq2 RNA-Seq were downloaded from Tabula Muris (https://figshare.com/articles/dataset/Single-cell_RNA-seq_data_from_Smart-seq2_sequencing_of_FACS_sorted_cells_v2_/5829687). Data was processed using Seurat v3. Cells were first filtered for number of unique genes (nFeature_RNA > 200,<3000), and log normalized (normalization.method=”LogNormalize’, scale.factor = 10000). Data were then clustered by non-linear dimensional reduction (UMAP/tSNE) to identify cell type, using published RDS file to preserve clusters. Differentially expressed features (genes) were identified using with FindMarkers. The top 300 genes that were positively associated as markers of each cluster (that is, had increased rather than decreased expression) were used to generate cell-type specific gene lists. Marker gene lists and according statistics for each cell subset are shown in Supplementary file 1. To create bed files to generate enrichment tracks for ATACSeq, mitochondrial genes were removed from cell-type specific gene sets, since these genes are accessible in all cell types. The characteristic genes for each major cardiac cell type (cardiomyocyte, endothelial, fibroblast) were used for to analyze and compare accessibility of cell-type specific gene sets.

Velocyto

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To quantify spliced and unspliced reads across cardiac cell populations, we analyzed BAM alignment files from Tabula Muris using Velocyto (La Manno et al., 2018). Cells within cardiomyocyte, endothelial, or fibroblast cell populations were selected using the published annotations in Tabula Muris. For higher coverage, we used the SMART-Seq2 data from the microwell experiments. Alignment was performed using STAR. Resulting BAM files were first processed using Velocyto.py with run_smart-seq2 settings to generate loom files for each population. Spliced and unspliced counts were extracted using the Veloctyo.R analysis pipeline, and summed on a per-gene basis across all cells within each population.

Primary mouse EC isolation

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ECs were isolated from CDH5-Cre/NuTRAP animals from heart or lung as previously described (Sawada et al., 2008). In brief, tissues were minced, and digested in 2 mg/mL of collagenase I in DMEM for 20 min at 37°C. Cell suspensions were filtered through 40 µM filtered, and incubated with CD31-conjugated magnetic dynabeads for 1 hr at 4°C. Beads were washed on a magnetic column 8–10 times, and bead-bound cells were grown on gelatin coated tissue culture plates. For ATACSeq of isolated ECs, cells isolated from one animal (heart or lung) were passaged two to four times and grown to confluency for at least 2 days before transposition.

Immunohistochemistry and confocal imaging

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For histology, hearts were prepared for paraffin embedding and sectioned. Samples were hybridized for Tnnt2 and Cdh5 RNAs using probes and target amplification by ACD (RNAScope Multiplex Florescent Assay v2 kit), and co-stained by for Pecam1 (Cd31) protein. Imaging was conducted by laser scanning confocal microscopy using a Zeiss LSM 710 Confocal microscope. Images were taken at 63X. Z-stacks were taken at 0.48 um apart. For representative images, three slices are merged and shown (final thickness 0.96 µm).

Statistics

Pair-wise comparisons were analyzed in Prism by two-tailed unpaired Student’s t-test, and p<0.05 was considered statistically significant. For sequencing analysis, statistics were performed using the programs outlined above.

Data availability

The endothelial translatome RNA-Seq data for Rpl22/Tek tissues from Cleuren, et al (2019) was extracted from Gene expression Omnibus (GEO) under accession number GSE138630. Microarray expression data of microvascular endothelial cells isolated by surface marker staining (Nolan et al, 2013) was retrieved from GSE47067. Microarray expression data from Coppiello, et al 2015, of Tie2-GFP labeled endothelial cells was obtained under GSE48209. The human fetal endothelial cell RNASeq data-set from Marcu, et al 2018 was obtained under accession GSE114607. Tabula Muris data is available under accession GSE109774, or on FigShare (https://figshare.com/projects/Tabula_Muris_Transcriptomic_characterization_of_20_organs_and_tissues_from_Mus_musculus_at_single_cell_resolution/27733).

The following data sets were generated
    1. Yucel N
    2. Axsom J
    3. Yang Y
    4. Li L
    5. Rhoades JH
    6. Arany Z
    (2020) NCBI Gene Expression Omnibus
    ID GSE144839. Cardiac endothelial cells maintain open chromatin and expresion of cardiomyocyte myofibrillar genes.
The following previously published data sets were used
    1. Cleuren ACA
    2. van der Ent MA
    3. Jiang H
    4. Hunker KL
    (2019) NCBI Gene Expression Omnibus
    ID GSE138630. The in vivo endothelial cell translatome is highly heterogeneous across vascular beds.
    1. Coppiello G
    2. Collantes M
    3. Sirerol-Piquer MS
    4. Vandenwijngaert S
    (2015) NCBI Gene Expression Omnibus
    ID GSE48209. Meox2/Tcf15 heterodimers program the heart capillary endothelium for cardiac fatty acid uptake.
    1. Nolan DJ
    2. Ginsberg M
    3. Israely E
    4. Palikuqi B
    (2013) NCBI Gene Expression Omnibus
    ID GSE47067. Molecular signatures of tissue-specific microvascular endothelial cell heterogeneity in organ maintenance and regeneration.
    1. Marcu R
    2. Choi YJ
    3. Xue J
    4. Fortin CL
    (2018) NCBI Gene Expression Omnibus
    ID GSE114607. Human Organ-Specific Endothelial Cell Heterogeneity.
    1. Tabula Muris Consortium
    (2018) NCBI Gene Expression Omnibus
    ID GSE109774. Tabula Muris: Transcriptomic characterization of 20 organs and tissues from Mus musculus at single cell resolution.

References

Decision letter

  1. Victoria L Bautch
    Reviewing Editor; University of North Carolina, Chapel Hill, United States
  2. Anna Akhmanova
    Senior Editor; Utrecht University, Netherlands

In the interests of transparency, eLife publishes the most substantive revision requests and the accompanying author responses.

Acceptance summary:

Your demonstration that myofibrillar genes are transcribed in cardiac endothelial cells using epigenetic, RNAseq and RNAscope/imaging criteria are appropriate for a Research Advance.

Decision letter after peer review:

Thank you for submitting your article "Cardiac endothelial cells maintain open chromatin and expression of cardiomyocyte myofibrillar genes" for consideration by eLife. Your article has been reviewed by three peer reviewers, and the evaluation has been overseen by a Reviewing Editor and Anna Akhmanova as the Senior Editor. The reviewers have opted to remain anonymous.

The reviewers have discussed the reviews with one another and the Reviewing Editor has drafted this decision to help you prepare a revised submission.

The editors have judged that your manuscript is of interest and potentially valuable to the field, but as described below, additional experiments are required before it is considered for publication. We would like to draw your attention to changes in our revision policy that we have made in response to COVID-19 (https://elifesciences.org/articles/57162). First, because many researchers have temporarily lost access to the labs, we will give authors as much time as they need to submit revised manuscripts. We are also offering, if you choose, to post the manuscript to bioRxiv (if it is not already there) along with this decision letter and a formal designation that the manuscript is 'in revision at eLife'. Please let us know if you would like to pursue this option. (If your work is more suitable for medRxiv, you will need to post the preprint yourself, as the mechanisms for us to do so are still in development.)

Summary:

Yucel and colleagues propose that cardiac endothelial cells (EC) have an epigenetic and transcriptional profile that partly mirrors that of cardiomyocytes. Specifically, the authors report the expression of cardiomyocyte myofibril genes (e.g., troponin, titin), which are present in cardiac endothelial cells but not in endothelial cells from other organs. The chromatin of these genes is in an "open state" , which appears to be dependent on the cardiac micro-environment. The authors speculate that this tissue-specific gene expression signature may reflect the developmental origin of cardiac endothelial cells. This work is provocative, and as such reviewers felt that more rigorous work is needed to support the conclusions.

Essential revisions:

Essential revisions focus primarily on rigorous experimentation to more unambiguously show that the "cardiac gene expression signature" is indeed cell-intrinsic to EC. It was pointed out that the presence of cardiomyocyte RNAs in the EC single-cell and ATAC seq data might be due to contamination, and that more rigorous data is needed to show in situ endothelial cell expression of cardiomyocyte genes unambiguously. There are also numerous instances of vague descriptions of approaches, methods and analysis that need to be improved. Essential points:

1) Several reviewers were concerned with the possibility that cardiomyocyte RNAs could be represented in the single-cell datasets but not originate from endothelial cells. They suggested the possibility of cardiomyocyte-EC doublets, or cell-free RNA sticking to antibodies or beads, or to glycocalyx of the EC cells. They point out that the ATAC-seq data is on bulk RNA and so could be contaminated by some cardiomyocytes, and that only the most abundant cardiomyocyte RNAs seem to be expressed by EC and represented in the ATAC-seq data. It was difficult to evaluate the interpretation of the data because the negative population in the TRAP experiment is not clearly defined, and if whole heart tissue is open to contamination from cardiomyocytes. Therefore more rigorous experimentation is required, such as dual single-cell RNA and ATAC-seq to show that bona fide EC have epigenetic patterns suggestive of cardiomyocyte gene expression. It would also strengthen the data to do single-cell RNA seq analysis before and after RNAse treatment to evaluate potential capture of negatively charged RNA by positively charged EC glycocalyx. It was suggested that a better analysis of the epigenetic landscape of cardiac genes in EC would be informative – for example, the chromatin status of other cardiac genes not found in heart EC is of interest. What about epigenetic marks that indicate active transcription of genes? Are these marks found in genes like Tnni, Tnnt2, Tnnc1 an Myh6 when analyzed in cardiac endothelial cells? And why closed chromatin is found in active genes (Figure 5) is not well-explained.

2) The RNA scope data was insufficient to determine whether the signals only originate from EC. This needs to be higher resolution and accompanied by antibody staining of EC-specific junction markers to clearly show EC cell borders. It was also suggested that antibodies to the cardiac proteins expressed by EC should be used with EC markers and high-resolution imaging, since the RNAs appear to be abundant (Figure 1).

3) There are numerous places where the methodology is vague, and where approaches and analysis are poorly described or documented.

[Editors' note: further revisions were suggested prior to acceptance, as described below.]

Thank you for resubmitting your work entitled "Cardiac endothelial cells maintain open chromatin and expression of cardiomyocyte myofibrillar genes" for further consideration by eLife as a Research Advance. Your revised article has been reviewed by two peer reviewers and the evaluation has been overseen by Anna Akhmanova as the Senior Editor and a Reviewing Editor.

The manuscript has been significantly improved, but there are some remaining issues that need to be addressed before acceptance, as outlined below:

1) The RNA scope data is much improved, but some concerns regarding specificity remain. Thus we request a Z-plane view of the CDH5/TNNT2 overlap from the RNA scope data – to document that the overlap extends to the Z plane. This should be doable from the images at hand.

2) We ask that authors amend the Discussion to better reflect the idea that the work focuses on transcriptional and epigenetic regulation of myocardial genes in cardiac endothelial cells, and that protein translation/function remains an open question.

https://doi.org/10.7554/eLife.55730.sa1

Author response

Essential revisions:

Essential revisions focus primarily on rigorous experimentation to more unambiguously show that the "cardiac gene expression signature" is indeed cell-intrinsic to EC. It was pointed out that the presence of cardiomyocyte RNAs in the EC single-cell and ATAC seq data might be due to contamination, and that more rigorous data is needed to show in situ endothelial cell expression of cardiomyocyte genes unambiguously. There are also numerous instances of vague descriptions of approaches, methods and analysis that need to be improved. Essential points:

1) Several reviewers were concerned with the possibility that cardiomyocyte RNAs could be represented in the single-cell datasets but not originate from endothelial cells. They suggested the possibility of cardiomyocyte-EC doublets, or cell-free RNA sticking to antibodies or beads, or to glycocalyx of the EC cells. They point out that the ATAC-seq data is on bulk RNA and so could be contaminated by some cardiomyocytes, and that only the most abundant cardiomyocyte RNAs seem to be expressed by EC and represented in the ATAC-seq data. It was difficult to evaluate the interpretation of the data because the negative population in the TRAP experiment is not clearly defined, and if whole heart tissue is open to contamination from cardiomyocytes. Therefore more rigorous experimentation is required, such as dual single-cell RNA and ATAC-seq to show that bona fide EC have epigenetic patterns suggestive of cardiomyocyte gene expression. It would also strengthen the data to do single-cell RNA seq analysis before and after RNAse treatment to evaluate potential capture of negatively charged RNA by positively charged EC glycocalyx. It was suggested that a better analysis of the epigenetic landscape of cardiac genes in EC would be informative – for example, the chromatin status of other cardiac genes not found in heart EC is of interest. What about epigenetic marks that indicate active transcription of genes? Are these marks found in genes like Tnni, Tnnt2, Tnnc1 an Myh6 when analyzed in cardiac endothelial cells? And why closed chromatin is found in active genes (Figure 5) is not well-explained.

We thank the reviewers for their critical evaluation of this important question of whether cross contamination between cardiomyocytes and ECs could explain the presence of myofibril gene RNAs in ECs. We, too, were very concerned about this possibility at first. We wish to clarify our ATAC-Seq data and RNAseq data, and better articulate why such cross-contamination cannot explain our findings:

1) “ATAC-seq data is on bulk RNA and so could be contaminated by some cardiomyocytes”: the ATAC-seq studies were performed on DNA, not RNA. In addition, for ATAC-Seq, it is important to note that the Tn5 transposase is specific for double stranded DNA, and thus will not be affected by cell-free RNA.

2) “Cardiomyocyte-EC doublets”: at the level of cells (i.e. Tabula Muris data) this is highly unlikely, as few cardiomyocytes were sorted in this dataset, due to their large size. At the level of nuclei sorting (our data), endothelial cell nuclei were sorted on the basis of DAPI staining, and doublets were excluded during sorting by both DAPI staining and FSC-A/FSC-H gating by flow cytometry. This is shown in a newly added Figure 3—figure supplement 1. We also assessed the exclusion of cardiomyocyte nuclei by co-staining of nuclei for PCM1, a specific marker of cardiomyocyte nuclei, and find no PCM1 signal in our EC nuclei fraction (Figure 3A). It is thus highly unlikely that doublets were included, and even if so, the numbers would be low.

3) “Only the most abundant cardiomyocyte RNAs seems to be expressed by EC and represented in the ATAC-seq data”. It is important to note that the ATAC-seq data evaluate chromatin, not RNA expression. Thus, contamination would have to occur at the level of chromatin DNA, not RNA, and the level of expression in cardiomyocytes would have no bearing on the ATAC-seq data. So, for example, if 10% of EC nuclear prep was contaminated by cardiomyocyte nuclei, then we would detect 10% open chromatin at the troponin genome locus, no matter the level of expression of troponin. Instead, we find 100% open chromatin at the troponin locus in the EC prep (Figure 4A). Contamination by CM nuclei cannot explain this observation.

4) “Cell-free RNA sticking to antibodies or beads, or to glycocalyx of the EC cells.” We do not exclude the contribution of contamination from cardiomyocyte RNA, which is inevitable in any RNA-Seq study. However, we performed analyses to demonstrate that such contamination, to the extent that it does occur, is minimal. First, we find that not all abundant cardiomyocyte RNAs are expressed in ECs, while, if contamination was indeed occurring, then all abundant RNAs should be detected, in relative proportion to their abundance. To quantify this observation, we followed the same approach as Jambusaria, et al., 2020, and performed Kendall-Tau rank-score statistical analysis on the highest 300 expressed genes in EC vs. non-EC material. We performed this comparison on both our own TRAP data, as well as TRAP data recently published in Cleuren, et al., 2019. In both our own data, as well as that from Cleuren, et al., there was a low correlation (<0.25), indicating that the ordering of highly expressed genes, of which many are CMF genes, is only poorly correlated in EC vs. non-EC samples. We clarify this important point in the text and new Figure 1B.

5) “Cell-free RNA sticking to antibodies or beads, or to glycocalyx of the EC cells.” The single cell RNAseq datasets demonstrate this not to be the case, as only a subset of ECs contained only a subset of CMF genes (most often only one) (Figure 2B). If CMF mRNAs were being captured by ECs (e.g. by positively charged EC glycocalyx), this would be observed in all cells; and even if only a subset of ECs captured CMF mRNAs (e.g. if only some ECs remained covered with glycocalyx) they would capture all CMF genes instead of on average only one. In addition, a glycocalyx explanation cannot explain the observations made with ribosome pulldowns, as seen in our data and that of Jambusaria et al., and that of Cleuren et al.

6) “The chromatin status of other cardiac genes not found in heart EC is of interest”. We agree. Strikingly, the majority of cardiomyocyte-specific genes reveal open chromatin in ECs (new Figure 4—figure supplement 1A) in particular in promoter regions (Figure 4C). Nevertheless, quantitatively, there are some cardiomyocyte-specific enhancer regions (intronic or intergenic) associated with CMF genes that are not found in endothelial cells (new Figure 4—figure supplement 1B). We show a few examples, including an enhancer region upstream of the gene encoding the cardiac transcription factor Nkx2-5, and an intronic enhancer within the Dmd gene (new Figure 4—figure supplement 1C). Once again, contamination cannot explain these data.

7) Finally, to provide yet more evidence that the expression of CMF genes in ECs is cell autonomous, we have quantified spliced vs. un-spliced reads, reasoning that if contamination were occurring, few un-spliced CMF mRNAs (i.e. nascent mRNAs) would be detected in ECs. Instead, we find relatively more un-spliced CMF mRNAs in ECs (new Figure 2D-E, Supplementary file 2), again supporting the notion that these mRNAs are expressed directly from open chromatin in the ECs themselves.

In summary, we provide extensive demonstration to address the fundamental question being posed of whether RNAs or DNAs originating from cardiomyocytes are “contaminating” the observed EC-specific RNAseq and ATACseq. We now articulate in the Discussion in more detail the numerous reasons why this cannot be the case, with the following paragraph:

“(1) Kendall Tau correlations demonstrate lack of correlation between genes highly expressed in cardiomyocytes and those potentially “contaminating” ECs. Were contamination to be occurring, these levels of expression would correlate. […] Thus, if contamination were occurring in this setting, EC chromatin would be, on average, only mildly open (proportional to the level of contamination), because the majority of the signal would still stem from ECs.”

2) The RNA scope data was insufficient to determine whether the signals only originate from EC. This needs to be higher resolution and accompanied by antibody staining of EC-specific junction markers to clearly show EC cell borders. It was also suggested that antibodies to the cardiac proteins expressed by EC should be used with EC markers and high-resolution imaging, since the RNAs appear to be abundant (Figure 1).

We provide new RNAscope data, as suggested, including higher resolution images, and accompanied by antibody staining of EC-specific junction maker Pecam1 (new Figure 3D). Additional images are also provided in Figure 3—figure supplement 2. We find that the proportion of ECs with detectable nuclear Tnnt2 mRNA on RNAscope is nearly identical to the proportion of ECs in the Tabular Muris data that express Tnnt2 (Quantification now moved to Figure 3—figure supplement 2D).

3) There are numerous places where the methodology is vague, and where approaches and analysis are poorly described or documented.

Methodologies and explanation of analyses have been expanded to be made more clear throughout.

[Editors' note: further revisions were suggested prior to acceptance, as described below.]

The manuscript has been significantly improved, but there are some remaining issues that need to be addressed before acceptance, as outlined below:

1) The RNA scope data is much improved, but some concerns regarding specificity remain. Thus we request a Z-plane view of the CDH5/TNNT2 overlap from the RNA scope data – to document that the overlap extends to the Z plane. This should be doable from the images at hand.

We have added X and Y views to the RNAscope images in order to better show overlap in the CD31+ regions.

2) We ask that authors amend the Discussion to better reflect the idea that the work focuses on transcriptional and epigenetic regulation of myocardial genes in cardiac endothelial cells, and that protein translation/function remains an open question.

We have added lines in the Discussion to emphasize that our work focuses on epigenetics and transcription, and that translation and function are questions that remain to be answered.

https://doi.org/10.7554/eLife.55730.sa2

Article and author information

Author details

  1. Nora Yucel

    Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States
    Contribution
    Conceptualization, Formal analysis, Investigation, Visualization, Writing - original draft
    For correspondence
    ndyucel@pennmedicine.upenn.edu
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-1848-6462
  2. Jessie Axsom

    Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States
    Contribution
    Data curation, Formal analysis, Investigation, Visualization, Writing - review and editing
    Competing interests
    No competing interests declared
  3. Yifan Yang

    Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States
    Contribution
    Formal analysis, Investigation, Methodology
    Competing interests
    No competing interests declared
  4. Li Li

    Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States
    Contribution
    Resources, Investigation, Methodology
    Competing interests
    No competing interests declared
  5. Joshua H Rhoades

    Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States
    Contribution
    Formal analysis, Supervision, Investigation, Methodology, Writing - original draft
    Competing interests
    No competing interests declared
  6. Zoltan Arany

    Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States
    Contribution
    Conceptualization, Data curation, Formal analysis, Supervision, Funding acquisition, Investigation, Methodology, Writing - original draft, Writing - review and editing
    For correspondence
    zarany@pennmedicine.upenn.edu
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-1368-2453

Funding

National Institute of Diabetes and Digestive and Kidney Diseases (R01HLDK114103)

  • Zoltan Arany

American Heart Association (AHA/Allen Initative)

  • Zoltan Arany

National Institutes of Health (T32 DKO7314)

  • Nora Yucel

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Acknowledgements

ZA is supported by NIH R01HLDK114103 and the AHA/Allen Foundation. NY was supported by NIH T32 DKO7314.

Ethics

Animal experimentation: This study was performed in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. All of the animals were handled according to approved institutional animal care and use committee (IACUC) protocols (#805255) of the University of Pennsylvania.

Senior Editor

  1. Anna Akhmanova, Utrecht University, Netherlands

Reviewing Editor

  1. Victoria L Bautch, University of North Carolina, Chapel Hill, United States

Publication history

  1. Received: February 4, 2020
  2. Accepted: December 13, 2020
  3. Accepted Manuscript published: December 14, 2020 (version 1)
  4. Version of Record published: December 23, 2020 (version 2)

Copyright

© 2020, Yucel et al.

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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    Chet Huan Oon, Kenneth E Prehoda
    Research Advance Updated

    The Par complex dynamically polarizes to the apical cortex of asymmetrically dividing Drosophila neuroblasts where it directs fate determinant segregation. Previously, we showed that apically directed cortical movements that polarize the Par complex require F-actin (Oon and Prehoda, 2019). Here, we report the discovery of cortical actomyosin dynamics that begin in interphase when the Par complex is cytoplasmic but ultimately become tightly coupled to cortical Par dynamics. Interphase cortical actomyosin dynamics are unoriented and pulsatile but rapidly become sustained and apically-directed in early mitosis when the Par protein aPKC accumulates on the cortex. Apical actomyosin flows drive the coalescence of aPKC into an apical cap that depolarizes in anaphase when the flow reverses direction. Together with the previously characterized role of anaphase flows in specifying daughter cell size asymmetry, our results indicate that multiple phases of cortical actomyosin dynamics regulate asymmetric cell division.