(A) Uniform manifold approximation and projection (UMAP) plot of 29,406 myelin-depleted single cells from murine cerebral cortex. Colors represent endothelial cells (EC), microglia (Micro), …
Source data for Figure 1C.
(A) Workflow for the enrichment of blood vessel-associated cell types from cerebral cortex of juvenile (P10), adult (7–11 weeks), aged (18 months), and experimental autoimmune encephalomyelitis (EAE;…
(A) Violin plots displaying number of genes (nFeature_Gene), number of unique molecular identifiers (UMI; nFeature_UMI), and percentage of mitochondrial genes (percent.mito) in juvenile, adult, and …
(A–B) Uniform manifold approximation and projection (UMAP) plots of 10,741 adult and aged mouse ECs. Colors represent cell subclusters (A) or age groups (B), respectively. Box indicates ICAM-1+ …
Source data for Figure 2E.
(A–B) Uniform manifold approximation and projection (UMAP) plots of juvenile ECs. Colors represent EC subclusters (A) or cell cycle phases (B). (C) Trajectory analysis of juvenile ECs depicting …
(A) Scatter dot plot of differentially expressed genes (yellow dots, Padj <0.001) between ECs from adult and aged samples (407 genes). The 25 most significant genes for each sample (red dots) are …
(A) Uniform manifold approximation and projection (UMAP) plots depicting the expression of representative marker genes for each subcluster: Alkbh5 (Venous), Tmsb10 (CapV), Alpl (CapA), and Fbln5 …
Source data for panel E.
(A) Heatmap and expression dot plot for top differentially expressed genes between microglia and PVMs. Dot size represents the percentage of cells expressing each gene, and color keys indicate …
(A) Uniform manifold approximation and projection (UMAP) plots depicting the expression of the known myeloid cell markers Cd68, Fcgr3, Cx3cr1, and Aif1. Color key represents the scaled expression. (B…
(A) Uniform manifold approximation and projection (UMAP) plot of 1725 PVMs from juvenile, adult, and aged mice distributed into Lyve1+ and MHCII+ subclusters. (B) Split UMAP plots of PVMs from …
Source data for Figure 4H.
(A) MA plot of differentially expressed genes between Lyve1+ and MHCII+ PVM subtypes. Blue dots, p-adjusted value <0.001; Red dots, p-adjusted value <0.001, and Log2 fold change >0.5. (B) Violin …
(A) Uniform manifold approximation and projection (UMAP) plots of 11,810 ECs from adult and EAE mice. Colors represent each EC subcluster (left) or condition (right). (B) UMAP plots depict Icam1 and …
Source data for Figure 5D.
(A) Uniform manifold approximation and projection (UMAP) plots of 26,106 myelin-depleted cells from adult and EAE cerebral cortex. Colors represent each cell type. (B) Dot plot displaying the top …
(A) Bar plots indicating the frequency of EC subtypes in healthy adult and EAE brain. (B) Dot plot showing the expression of top subcluster-specific genes, with the dot size representing the …
Source data for panel A.
(A) Confocal images showing ICAM-1 immunofluorescence in cortex of control, EAE onset, and peak mice. Arrowheads indicate expansion of ICAM-1 staining from reactive endothelial venules (REVs) into …
(A) Volcano plots depicting differentially expressed genes between adult and EAE EC subtypes. Blue dots, p-adjusted value <0.001; red dots, p-adjusted value <0.001, and Log2 fold change >0.5. (B) …
(A) Representative immunohistochemistry images for CD206+ PVMs and ICAM-1+ vessels in control and EAE brain cortical parenchyma. Scale bars, 100 μm. (B) Frequency of CD206+ PVMs associated to ICAM-1+…
Source data for Figure 6B.
(A) Uniform manifold approximation and projection (UMAP) plot and pie chart of PVMs in adult and EAE brain cortex in the indicated cell cycle phases. (B) CD31 and CD206 immunostaining of brain …
(A) Overview of potential ligand-receptor interactions for CapV, reactive endothelial venule (REV), and venous EC populations in adult and experimental autoimmune encephalomyelitis (EAE) based on …
Overview of differential ligand-receptor interactions between reactive endothelial venule (REV) endothelial cell (EC), perivascular macrophage (PVM), mural cell (Mural), and astroependymal cells …
Schematic summary of proposed findings. Reactive endothelial venules (REVs) are specialized cellular gateways in the CNS vasculature with associated perivascular macrophages (PVMs) as gatekeepers in …