(A) Chart summarizing histone SUMOylation, phosphorylation and acetylation sites identified in this study, together with previously identified modifications. AA, amino acid position. (B) Temporal profiles of SUMOylation on K123 and K126 of histones H2A1 and H2A2. The K123 profiles are plotted on the right-hand side Y-axis in green. (C) Temporal profiles of K4 and K7 SUMOylation on histone H2A. (D) Temporal profile of histone H2B-K123 SUMOylation. (E) SUMO site intensities on histone H3. (F) Temporal profiles of histone H4 SUMOylation sites. (G) Site intensities of histone H1 SUMOylation sites plotted along its domain structure. Two sites that are co-modified with adjacent phosphorylation are indicated in red. (H) Summary of SUMOylated proteins involved in rDNA silencing. NTS1/2, non-transcribed sequences; RFB, replication fork barrier. (I) Normalized LFQ profiles of RENT complex components. (J) Cartoon illustrating the recruitment of rDNA loci to the inner nuclear membrane (INM) via RENT, cohibin (Csm1 and Lrs4), and CLIP (chromosome linkage inner-nuclear membrane proteins) complexes. INM, inner-nuclear membrane; RFB, replication fork barrier. (K) Normalized LFQ profiles of Cohibin (Csm1 and Lrs4) and Tof2. (L) Normalized LFQ profiles of the SIR proteins. (M) Site intensity plot of Sir4 plotted along its domain structure. (N) Normalized LFQ profiles of Sir4 and its binding partners. (O) Normalized LFQ profiles of Sir3 and its paralog Asf2. (P) LFQ profile of chromatin remodeler Chd1. (Q) Normalized LFQ profiles of SWR1-chromatin remodeler components Swc3 and Vps72 (R) Normalized LFQ profiles of chromatin remodeler components Bdf1 (SWR subunit that binds acetylated H4) and Taf14 (subunit of TFIID, TFIIF, INO80, Swi/Snf, and NuA3m that binds acetylated H3). (S) Normalized LFQ profile of Sum1, a component of the Sum1-Rfm1-Hst1 deacetylase that represses middle-meiotic genes. (T) Normalized LFQ profiles of Tup1, a constituent of the Tup1-Cyc8 transcriptional repressor, and the transcriptional activator Gcn4.