(A) Histogram of probabilities for association of IDRs with sequence-specific DNA binding (transcription factor or ‘TF’) function. Top predictions are indicated. From the top predictions, IDRs from known transcription factors are indicated with a green dot. Mfg1 is an uncharacterized protein. (B) Mfg1 protein coordinates, with all known domain annotations (via PFAM), disorder prediction (via DISOPRED3), and FAIDR probability for association with sequence-specific DNA binding indicated. (C) Feature vector (evolutionary signature) for Mfg1 IDR 1 (y-axis) is plotted against predictive features for transcription factor/sequence-specific DNA binding function as determined by FAIDR (x-axis). Log variance in glutamine (Q) residues is highlighted as one of the top features (orange circle) associated with TF function. Glutamine residues (Q) are indicated (in orange) in the IDR of Mfg1 and orthologs obtained from the YGOB (Byrne and Wolfe, 2005). All other (non-glutamine) residues are indicated in black. Multiple sequence alignment of orthologs is visualized using Jalview (Waterhouse et al., 2009). Species in alignment (from bottom to top) are as follows: Saccharomyces cerevisiae, Saccharomyces mikatae, Saccharomyces kudriavzevii, Saccharomyces uvarum, Candida glabrata, Kazachstania africana, Kazachstania naganishii, Naumovozyma castellii, Naumovozyma dairenensis, Zygosaccharomyces rouxii, Torulaspora delbrueckii, Eremothecium (Ashbya) gossypii, Eremothecium (Ashbya) cymbalariae, Lachancea kluyveri, Lachancea thermotolerans, and Lachancea waltii.