(A) The number of circulating hemocytes increased upon the loss of Rab5 or Rab11 but not Rab7 in hemocytes. (B–P) Immunostaining for PH3 was performed in lymph glands from third instar larvae. The …
(A–F, P) Lymph glands were labeled with DAPI to measure the anterior lobe area. The anterior lobe areas in different genotypes are shown in (P). (G–L, Q) Cell proliferation was analyzed in hemocytes …
(A–D”) The localization of Rab5 and Rab11 was analyzed in Hml>UAS-GFP and col>UAS-GFP lymph glands with anti-GFP (green) and anti-Rab5 (red) or anti-Rab11 (red) antibodies as appropriate. (E–H’) No …
(A–D) The percentage of the GFP-positive area in anterior lobes was increased in Hml>UAS-GFP>UAS-Rab5DN and Hml>UAS-GFP>UAS-Rab11DN lymph glands; the quantification is shown in (D). (E–G’) …
(A–C’) The plasmatocyte count was increased in the anterior (A–C) and posterior (A’–C’) lobes in lymph glands from Hml>UAS-Rab5 RNAi and Hml>UAS-Rab11 RNAi larvae, as determined by anti-P1 …
(A–H) P1 staining was performed in Hml>WT (A), Hml>UAS-Rab5/11DN>WT (B, E), Hml>UAS-Rab5/11DN>UAS-STAT92E (C, F), and Hml>UAS-Rab5/11DN>UAS-Adgf-A (D, G) lymph glands. The quantifications of the …
(A–D) The crystal cell count (as evaluated by anti-ProPO staining) was unchanged in lymph glands upon the inactivation of Rab5 or Rab11. (E–G) Aberrant lamellocyte formation was observed in Hml>UAS-G…
(A–O) Aberrant lamellocyte formation was observed in pxn>UAS-GFP>UAS-Rab5/11DN, He>UAS-GFP>UAS-Rab5/11DN, and srp>UAS-Rab5/11DN lymph glands and in pxn>UAS-GFP>UAS-Rab5/11DN and He>UAS-GFP>UAS-Rab5/1…
(A and B) Immunostaining of lamellocytes was performed with anti-L1 antibodies. The increased lamellocyte count was rescued in Hml>UAS-Rab5DN>UAS-Rab11CA lymph glands. Scale bar: 50 μm.
PSC cells and the MZ were analyzed with anti-Antp antibodies or labeled with GFP after the inactivation of Rab5 or Rab11 using the Hml-Gal4 (A–C, Y), dome-Gal4 (J–L, Z), Antp-Gal4 (S–U, AA), and col-…
(A–I) The JNK pathway activity was elevated in lymph glands upon Rab5 or Rab11 inactivation, as elucidated by the monitoring of JNK signaling with puc-lacZ (A–C) and anti-p-JNK antibodies (D–F). …
Knocking down hop (A–F) or overexpressing UAS-p35 (G–J) failed to repress the aberrant lamellocyte differentiation in Hml>UAS-GFP>UAS-Rab5/11 RNAi or Hml>UAS-Rab5/11DN lymph glands. Scale bar: 50 μm.
(A–D) Knocking down bsk repressed the aberrant lamellocyte differentiation in Hml>UAS-Rab5/11 RNAi circulating hemocytes. Quantification of the lamellocyte count is shown in (E). Scale bar: 20 μm. …
(A–C’’) An increased degree of Hrs (green) and p-JNK (red) colocalization was observed in Hml>UAS-Rab5DN and Hml>UAS-Rab11DN hemocytes. Merged images (Hrs+p-JNK+DAPI) are displayed in (A–C). (D–K) …
(A–U) Circulating hemocytes were stained with antibodies against p-JNK (A–G), p-Jun (H–N), and Mmp1 (O–U). Hml>UAS-hepAct, Rab5>UAS-bsk, and Rab11>UAS-bsk hemocytes showed elevated levels of p-JNK, …
(A–D) Hemocytes from Hml>ppl>UAS-bsk, Hml>NP3084>UAS-bsk, and Hml>elav>UAS-bsk larvae were stained with anti-L1 antibodies. The lamellocyte frequency in circulating hemocytes is shown in (D). Scale …
(A–F) Immunostaining of circulating hemocytes showed that the levels of p-Jun (A–C) and Mmp1 (D–F) were increased after the inactivation of Rab5 or Rab11. (G–M) The lamellocyte frequency, as …
(A–C) Immunostaining of lymph glands showed high p-Erk (red) signals upon Rab5 or Rab11 inactivation. (D–I) Anterior lobe enlargement (visualized by DAPI staining) in Hml>UAS-Rab5/11DN lymph glands …
(A–D) Knocking down Ras85D repressed the aberrant lamellocyte differentiation in Hml>UAS-Rab5/11DN circulating hemocytes. (E) The lamellocyte frequency in circulating hemocytes. Scale bar: 20 μm. …
(A–H) Immunostaining of circulating hemocytes showed that Dif and Dorsal were activated in Hml>UAS-Rab5/11DN (B–C, F–G) and Hml>UAS-hepAct (D, H) hemocytes. (I–X) Detection of lamellocyte formation …
The Dif (A–C) and Dorsal (D–F) levels were analyzed with immunostaining and found to be elevated in Hml>UAS-Rab5 RNAi and Hml>UAS-Rab11 RNAi hemocytes. Scale bar: 10 μm.
(A–C) Many autophagosomes were observed in Cg;Atg8a-mCherry>UAS-Rab5DN and Cg;Atg8a-mCherry>UAS-Rab11DN hemocytes. (D–I) Circulating hemocytes of Hml-Gal4, Hml>UAS-Rab5DN, and Hml>UAS-Rab11DN larvae …
(A and B) Overexpression of dFOXO in hemocytes using pxn>GFP induced lamellocyte formation. (C–F) The dFOXO (green) levels were increased in circulating hemocytes from Hml>UAS-Rab5DN, Hml>UAS-Rab11DN…
(A–F) The increased lamellocyte counts were restored in Hml>UAS-Rab5/11DN>UAS-bsk RNAi (B, E) and Hml>UAS-Rab5/11DN>UAS-bskDN (C, F) circulating hemocytes. The quantification of lamellocytes is …
(A–I, U–X) The lamellocyte count was determined in circulating hemocytes by anti-L1 immunostaining. Overexpression of Atg1 in two sources of UAS-Atg1 flies resulted in massive lamellocyte production …
(A) Schematic diagram of lymph glands from control and Rab5/Rab11-deficient third instar larvae. When Rab5 or Rab11 was downregulated in the cortical zone (CZ), the formation of many premature …