Antipsychotic olanzapine-induced misfolding of proinsulin in the endoplasmic reticulum accounts for atypical development of diabetes
Abstract
Second-generation antipsychotics are widely used to medicate patients with schizophrenia, but may cause metabolic side effects such as diabetes, which has been considered to result from obesity-associated insulin resistance. Olanzapine is particularly well known for this effect. However, clinical studies have suggested that olanzapine-induced hyperglycemia in certain patients cannot be explained by such a generalized mechanism. Here, we focused on the effects of olanzapine on insulin biosynthesis and secretion by mouse insulinoma MIN6 cells. Olanzapine reduced maturation of proinsulin, and thereby inhibited secretion of insulin; and specifically shifted the primary localization of proinsulin from insulin granules to the endoplasmic reticulum. This was due to olanzapine’s impairment of proper disulfide bond formation in proinsulin, although direct targets of olanzapine remain undetermined. Olanzapine-induced proinsulin misfolding and subsequent decrease also occurred at the mouse level. This mechanism of olanzapine-induced β-cell dysfunction should be considered, together with weight gain, when patients are administered olanzapine.
Introduction
Patients with schizophrenia are typically prescribed first- or second-generation antipsychotics (FGAs or SGAs, respectively). FGAs block dopamine signaling in the brain by inhibiting the function of its receptors, resulting in recovery from conditions such as acute mania and agitation (Divac et al., 2014). However, because of excessive inhibition of the dopamine pathway, FGAs can cause extrapyramidal symptoms, including dystonia, akathisia, and Parkinsonism, and are now widely replaced by SGAs due to their reduced risk of causing adverse extrapyramidal effects (Høiberg and Nielsen, 2006) and greater effectiveness in alleviating symptoms (Lee et al., 2002; Sirota et al., 2006). These effects of SGAs are caused by their inhibition of signaling through the dopamine D2 receptor and 5-HT2A serotonin receptor (Divac et al., 2014; Kasper et al., 1999). Of note, SGAs have higher risks of causing metabolically adverse side effects such as obesity (Gothelf et al., 2002), dyslipidemia (Gothelf et al., 2002) and diabetes mellitus (Citrome and Volavka, 2005; Koller and Doraiswamy, 2002).
The SGA olanzapine affects neurotransmitter receptors (called multi-acting receptor-targeted antipsychotics) and thereby exhibits significantly greater effectiveness in alleviating various symptoms than other SGAs (Sirota et al., 2006; van Bruggen et al., 2003). However, it carries a higher risk of diabetes than other SGAs such as risperidone, and its frequent use therefore represents a clinical problem (Deng, 2013; Newcomer, 2005; Rummel-Kluge et al., 2010). Generally, olanzapine-induced diabetes is attributed to weight gain caused by increased appetite through the effect on the feeding center via blocking of 5-HT2C receptor, leading to insulin resistance-mediated development of diabetes.
Nonetheless, olanzapine-induced diabetes includes atypical cases, as follows. First, while it usually takes years to develop diabetes via insulin resistance, olanzapine-induced diabetes occurs within 6 months after commencing treatment (Kinoshita et al., 2014; Nakamura et al., 2014; Nakamura and Nagamine, 2010). Second, discontinuing olanzapine treatment cures diabetes even after the level of HbA1c (a marker for blood glucose level) reaches >10%, a ratio which normally representing an irreversible level in patients with type I or type II diabetes (Nakamura et al., 2014; Nakamura and Nagamine, 2010; Nathan et al., 2009; Young et al., 2012). Moreover, diabetic ketoacidosis, which is caused by insulin hyposecretion, often occurs in patients with type I diabetes, but also rapidly (within 6 months after treatment with olanzapine) affects patients with no diabetic symptoms before medication (Kinoshita et al., 2014; Tsuchiyama et al., 2004). Indeed, epidemiological data show that patients receiving olanzapine incur an approximately 10-times higher risk of diabetic ketoacidosis than the general population (Polcwiartek et al., 2016), suggesting the possibility that olanzapine directly impairs the function of pancreatic β cells, the exclusive source of insulin, in a speculated few percent of medicated patients (Nagamine, 2014; Nagamine, 2018).
We are interested in the mechanism of the development of olanzapine-induced atypical diabetes from the viewpoint of the protein quality control system operating in the endoplasmic reticulum (ER). Secretory and membrane proteins gain their tertiary and quaternary structures in the ER, which is assisted by ER-localized molecular chaperones, glycosyltransferases and oxidoreductases (collectively referred to here as ER chaperones). Only correctly folded molecules are transported from the ER to the Golgi apparatus and then to their destination. However, proteins that fail to fold correctly, even with the assistance of ER chaperones, are retrotranslocated into the cytosol and degraded by the ubiquitin-proteasome system (Qi et al., 2017). This disposal system is called ER-associated degradation (ERAD).
Although eukaryotic cells perform quality control of proteins through ER chaperone-assisted productive folding and ERAD, the requirements for protein synthesis may exceed the protein folding capacity of the ER under physiological and pathological conditions. To counteract such ER stress, namely the accumulation of unfolded or misfolded proteins in the ER, the unfolded protein response (UPR) is triggered, leading to the activation of three ER transmembrane sensor proteins PERK, ATF6, and IRE1. Interestingly, their activation effects come out sequentially. First, phosphorylation of the α subunit of eukaryotic translation initiation factor 2 (eIF2α) by activated PERK transiently suppresses translation (see Figure 1A) so that the burden of the ER is mitigated because of reduced translocation of newly synthesized proteins into the ER. Next, activated ATF6 upregulates the transcription of ER chaperone genes to increase the folding capacity of the ER. Subsequently, activated IRE1 together with activated ATF6 upregulates the transcription of ERAD component genes, which increases degradation capacity. If ER stress is prolonged, apoptotic signaling is activated (Mori, 2000; Tabas and Ron, 2011; Yamamoto et al., 2007; Yoshida et al., 2003).

Effect of olanzapine on secretion of insulin from MIN6 cells.
(A) Schematic representation of protein quality control in the ER, PERK-mediated translational attenuation, and storage and secretion of insulin (see Introduction). GSK2656157 inhibits protein kinase activity of PERK. (B) Scheme for measuring the level of insulin secreted into medium in response to glucose stimulation. After culture in a medium containing 25 mM glucose, MIN6 cells were starved for 1 hr in medium containing 3 mM glucose (Stav), and then cultured in medium containing 25 mM glucose (Stim or 3 mM glucose as control). The amount of insulin secreted into the medium during the 1 hr incubation was determined using an ELISA. The data are normalized to the amounts of total cellular proteins and presented as the mean ± SD (n = 2). (C) MIN6 cells were pretreated with the indicated concentrations of olanzapine (OLA) for 4 hr or 14 hr in medium containing 25 mM glucose before glucose starvation as shown in (B). (D) MIN6 cells were pretreated with 50 μM olanzapine or risperidone (RIS) for 14 hr before glucose starvation as shown in (B). (E) Schematic representation of the signaling cascade for insulin secretion in response to glucose stimulation. Cells intake glucose (①). Glucose metabolism (②) triggers an increase in cellular ATP/ADP ratio (③), which inhibits K+ channels and induces depolarization (④). This leads to opening of Ca2+ channels (⑤) and increased influx of Ca2+ (⑥), inducing the fusion of insulin granules to the plasma membrane for secretion (⑦). Mastoparan (Mast) enhances insulin secretion by increasing the intracellular concentration of Ca2+ independently of glucose intake. (F) MIN6 cells were pretreated with 50 μM olanzapine or risperidone for 14 hr before glucose starvation as shown in (B). Mastoparan (Mast, 20 μM) was added at the time of glucose stimulation to aliquots of cells not pretreated or pretreated with 50 μM olanzapine for 14 hr.
Pancreatic β cells synthesize preproinsulin, fold proinsulin in the ER, and store large quantities of insulin in insulin granules (see Figure 1A). In these roles, they are frequently subject to ER stress, and the UPR accordingly plays a critical role in maintaining homeostasis. Immediate translational control mediated by the PERK pathway, rather than time-consuming transcriptional control of ER chaperones etc. (Yoshida et al., 2003), is particularly important for the folding of proinsulin, a very small molecule compared with ER chaperones; when translation is generally attenuated by the activation of PERK, stochastically unfolded or misfolded proinsulin can be efficiently refolded by the action of preexisting ER chaperones. If PERK is not active, synthesis of misfolded proinsulin continues, leading to proteotoxicity-mediated apoptosis of β cells. Accordingly, PERK knockout mice develop diabetes after birth (Harding et al., 2001). Further, PERK mutation causes human Wolcott-Rallison syndrome, which induces diabetes in infancy (Delépine et al., 2000). We previously found that olanzapine induces mild ER stress in hamster pancreatic β-cell line HIT-T15, which secretes insulin. However, phosphorylation of eIF2α is not elevated despite mild activation of PERK in olanzapine-treated HIT-T15 cells, leading to sustained protein synthesis followed by induction of apoptosis, although we used olanzapine at the concentration of 100 μM (Ozasa et al., 2013).
We also previously found that olanzapine markedly inhibits insulin secretion by HIT-T15 cells (Ozasa et al., 2013). In the present study, we confirmed that 10–50 μM olanzapine inhibits insulin secretion by the mouse pancreatic β-cell line MIN6, which is frequently used to study β cells. We then investigated the stage of protein quality control at which insulin secretion is blocked in olanzapine-treated MIN6 cells, and then whether the identified stage is indeed compromised in islets of mice after daily oral administration of olanzapine.
Results
Inhibition of insulin secretion in olanzapine-treated MIN6 cells
MIN6 cells exhibit an approximately 7-fold increase in insulin secretion in 2 hr when the extracellular glucose concentration is increased from 5 to 25 mM (Ishihara et al., 1993). Here, we detected an approximately 4-fold increase in insulin secretion in 1 hr when the extracellular glucose concentration was increased from 3 to 25 mM (stimulation after starvation for 1 hr, Figure 1B) compared with continuous incubation in 3 mM glucose (Figure 1C, compare bar 2 with bar 1). Glucose-stimulated insulin secretion was inhibited in a concentration-dependent manner when MIN6 cells were first treated with 25 mM glucose in the presence of 10, 20, or 50 μM olanzapine for 4 hr (Figure 1C, bars 3–5) and was markedly inhibited by pretreatment for 14 hr with these same concentrations of olanzapine (Figure 1C, bars 6–8), but not by pretreatment for 14 hr with 50 μM risperidone (Figure 1D, compare bar 4 with bar 2). Pretreatment was essential for this effect, because the addition of 50 μM olanzapine from the beginning of the 1 hr starvation did not detectably inhibit glucose-stimulated insulin secretion (data not shown), suggesting that it takes time for olanzapine to exert its inhibitory effect on insulin secretion.
Glycolysis causes ATP-mediated closure of the potassium (K+) channels, leading to opened calcium (Ca2+) channel-mediated influx of extracellular Ca2+. The increase in cytosolic Ca2+ concentrations culminates in fusion of insulin granules with the plasma membrane and subsequent secretion of insulin (Figure 1E; Rorsman and Braun, 2013). Therefore, insulin secretion can be induced by mastoparan, which increases intracellular Ca2+ concentrations by activating phospholipase C to produce inositol 1, 4, 5-triphosphate (Perianin and Snyderman, 1989). Indeed, treatment of MIN6 cells with mastoparan for 1 hr after glucose starvation rapidly stimulated insulin secretion (Figure 1F, compare bar 2 with bar 1). Pretreatment of MIN6 cells with 50 μM olanzapine for 14 hr partially inhibited mastoparan-induced insulin secretion (Figure 1F, compare bar 5 with bar 2). These results suggest that olanzapine may not block Ca2+-induced fusion of insulin granules with plasma membrane, but may decrease the amount of insulin stored in insulin granules.
Retention of proinsulin in the ER in olanzapine-treated MIN6 cells
Proinsulin produced from preproinsulin by the action of signal peptidase becomes mature proinsulin via formation of three intramolecular disulfide bonds in the ER, and is then processed to insulin (Figure 2A). To analyze MIN6 cells which secrete both proinsulin and insulin (Lee et al., 2011; Tsuchiya et al., 2018), as is seen when isolated mouse and human islets were analyzed by pulse-chase experiments (Dufurrena et al., 2019), we employed two types of mouse monoclonal antibodies with different characteristics, namely #8138 raised against a synthetic peptide corresponding to the residues surrounding Val36 of human insulin, which are conserved in mouse insulin (yellow circle denotes Val36 in Figure 2A), and I2018 raised against human insulin. Under reducing conditions, #8138 detected mainly proinsulin in lysates of MIN6 cells, and both proinsulin and insulin B chain in lysates of mouse islets, probably reflecting their relative contents (Figure 2B). It should be noted that #8138 was 800-fold more reactive with purified recombinant human proinsulin than purified recombinant human insulin (insulin B chain) under reducing conditions (Figure 2C), and that #8138 reacted with neither proinsulin nor insulin under non-reducing conditions (Figure 2B).

Characterization of two anti-insulin monoclonal antibodies utilized.
(A) Schematic representation of maturation of insulin 2. Each circle denotes an amino acid residue. Preproinsulin 2 comprises a signal sequence (light gray), B chain (blue), C peptide (gray), and A chain (green). Preproinsulin 2 is converted to proinsulin 2 after cleavage of the signal sequence, and proinsulin 2 becomes mature proinsulin 2 via formation of three intramolecular disulfide bonds. Mature proinsulin 2 is converted to insulin 2 after proteolysis at the residues indicated by the two arrows. The red circles represent cysteine residues that form the disulfide bonds (bars), and which are required for insulin folding and activity. The region encompassing V36, indicated by the yellow circle, is recognized by the anti-insulin monoclonal antibody #8138, which immunoprecipitates (immature) proinsulin 2 and detects proinsulin 2 and insulin 2 (B chain) after reducing SDS-PAGE. In contrast, the anti-insulin monoclonal antibody I2018 immunoprecipitates mature proinsulin 2 and insulin 2, and detects insulin 2 after non-reducing SDS-PAGE. (B) Lysates of MIN6 cells and mouse islets were analyzed by immunoblotting using #8138 and I2018 after reducing (DTT +) and non-reducing (DTT -) SDS-PAGE. (C) The indicated amounts of recombinant and purified proinsulin and insulin were subjected to reducing SDS-PAGE followed by immunoblotting using #8138.
A pulse (20 min)-chase (80 min) experiment showed that the immunoprecipitates obtained from MIN6 cells using #8138 were detected as doublet bands which migrated slightly faster than the 8.5 kDa marker and were secreted into medium within the 80 min chase (Figure 3A and B). On comparison of the migration positions of in vitro translational products, the upper band was considered to be proinsulin 2 (Figure 3B), consistent with the abundant expression of insulin 2 mRNA in MIN6 cells (Roderigo-Milne et al., 2002). The lower band was considered to be processed proinsulin 2, such as the B chain connected to the C peptide but not to the A chain as observed in isolated rat islets (Harding et al., 2012), or somewhat cleaved proinsulin, because only proinsulin 2 was detected after a shorter pulse (3 min) and processed proinsulin 2 appeared after the 30 min chase (Figure 3C). Processed proinsulin 2 is likely to be produced after moving out from the ER, because its level was markedly decreased by treatment with brefeldin A, an inhibitor of anterograde transport from the ER to the Golgi apparatus (Figure 3D). #8138 immunoprecipitated proinsulin 2 and processed proinsulin 2 but not insulin 2 from MIN6 cells after pulse (20 min)-chase (reducing conditions, Figure 3E).

Secretion of proinsulin 2, processed proinsulin 2 and insulin 2 from MIN6 cells.
(A) Schemes of the experiments shown in (B) and (C). (B) cDNAs encoding mouse preproinsulin 1, preproinsulin 2, proinsulin 1, and proinsulin 2 were subjected to in vitro transcription and translation in the presence of 35S-Met and 35S-Cys, and then to immunoprecipitation using #8138. A pulse (20 min)-chase (80 min) experiment was performed in MIN6 cells, and cell lysates and media were collected and subjected to immunoprecipitation using #8138. The immunoprecipitates were analyzed by reducing SDS-PAGE and autoradiography. (C) A pulse (3 or 20 min)-chase (30 min) experiment was performed in MIN6 cells, and cell lysates and media (M) were analyzed as in (B). (D) MIN6 cells untreated or treated with brefeldin A (BFA, 10 µg/ml) were subjected to pulse-chase experiment to determine changes in the levels of intracellular and extracellular proinsulin as well as processed proinsulin, as shown in the schema (top). (E) cDNAs encoding mouse proinsulin 2, B chain of mouse insulin 1, and B chain of mouse insulin 2 were treated as in (B). A pulse (20 min)-chase (40 min and 80 min) experiment was performed in MIN6 cells, and cell lysates and media were collected and subjected to immunoprecipitation using #8138 or I2018. The immunoprecipitates were analyzed by reducing and non-reducing SDS-PAGE followed by autoradiography.
In contrast, I2018 detected insulin in lysates of MIN6 cells and mouse islets only under non-reducing conditions (Figure 2B). A pulse (20 min)-chase experiment showed that I2018 immunoprecipitated both proinsulin 2 and insulin 2 from MIN6 cells (reducing conditions, Figure 3E). This also demonstrated the precursor-product relationship for proinsulin 2 and insulin 2 in MIN6 cells. Importantly, I2018 immunoprecipitated mature proinsulin 2, which folded into a compact structure and thereby migrated slightly faster than proinsulin 2 immunoprecipitated using #8138, in addition to insulin 2 (non-reducing conditions, Figure 3E). Hereafter, #8138 and I2018 were appropriately used in accordance with experimental purposes.
We examined the effect of olanzapine and risperidone on the synthesis and secretion of proinsulin and insulin in MIN6 cells by pulse-chase experiments (Figure 4A). Analysis under reducing conditions of immunoprecipitates obtained with #8138 showed that production of intracellular processed proinsulin (P’) as well as secretion of both proinsulin (P) and processed proinsulin (P’) were markedly decreased by treatment of MIN6 cells with 50 μM olanzapine (Figure 4B, lanes 8–14) but not with 50 μM risperidone (lanes 29–35) compared with control cells (lanes 1–7), and that proinsulin (P) was instead accumulated intracellularly in olanzapine-treated MIN6 cells [see quantified Figure 4B (a)]. These phenomena were observed when MIN6 cells were similarly treated with 10 μM olanzapine but not with 10 μM risperidone (Figure 5A and B). Analysis under non-reducing conditions of immunoprecipitates obtained with I2018 showed that the levels of both intracellular mature proinsulin (mP) and insulin (I) were markedly decreased and that secretion of both mature proinsulin (mP) and insulin (I) was markedly inhibited by treatment of MIN6 cells with 50 μM olanzapine (Figure 4B, lanes 22–28) but not with 50 μM risperidone (lanes 36–42) compared with control cells (lanes 15–21) [see quantified Figure 4B (b)], consistent with the ELISA data (Figure 1). Thus, olanzapine blocked maturation of proinsulin and accordingly inhibited secretion of insulin.

Effect of olanzapine and risperidone on maturation and secretion of proinsulin and insulin in MIN6 cells.
(A) Schemes of the experiments shown in (B). (B) MIN6 cells untreated or treated with olanzapine (50 µM) or risperidone (50 µM) from the start of starvation were subjected to pulse-chase experiment to determine changes in the levels of proinsulin (P), processed proinsulin (P’), mature prinsulin (mP), insulin (I), HMP-1 (1) and HMP-2 (2) in cells and medium. Cell lysates and media were collected and immunoprecipitated with #8138 or I2018. The immunoprecipitates were analyzed by reducing and non-reducing SDS-PAGE followed by autoradiography. The intensity of each band was determined, and the intensity of intracellular proinsulin or mature proinsulin at time 0 in control cells was defined as 100% (n = 3) [categorized in a, b and c].

Effect of the proteasome inhibitor MG132 on the level of proinsulin accumulated intracellularly in olanzapine-treated MIN6 cells.
(A) Schemes of the experiments shown in (B). (B) MIN6 cells untreated or treated with olanzapine (10 µM) or risperidone (10 µM) for 4 hr were analyzed as in Figure 4B to determine changes in the levels of proinsulin (P) and processed proinsulin (P’) in cells and medium using #8138 and reducing SDS-PAGE. The intensity of intracellular proinsulin at time 0 was defined as 100% (n = 3). (C) Schemes of the experiments shown in (D). (D) MIN6 cells untreated or treated with olanzapine (50 µM), MG132 (30 µM) or olanzapine (50 µM) and MG132 (30 µM) were analyzed as in Figure 4B to determine changes in the level of proinsulin (P) in cells and medium using #8138 and reducing SDS-PAGE. The intensity of intracellular proinsulin at time 0 was defined as 100% (n = 3).
Phogrin, a transmembrane protein-tyrosine phosphatase-like protein, transits the secretory pathway (Torii et al., 2005), and furin-like convertases convert proinsulin and phogrin to their mature forms by cleaving them at a dibasic consensus site (Hermel et al., 1999; Nakayama, 1997). Pulse-chase experiments showed that olanzapine did not detectably affect the processing of transfected phogrin-GFP (Figure 6A) or secretion of transfected α1-proteinase inhibitor (A1PI) (Figure 6B). The anti-KDEL antibody stained the ER by recognizing the major ER chaperones BiP/GRP78 and GRP94, and A1PI was widely distributed from the ER to the Golgi apparatus before and after olanzapine treatment (Figure 6C and D). Olanzapine also did not significantly affect the maturation of hemagglutinin from high-mannose type to complex type (Figure 6E), which requires correct disulfide bond formation for folding (Segal et al., 1992). These results confirmed the specificity of olanzapine’s effect to proinsulin.

Effect of olanzapine on processing of phogrin-GFP, secretion of A1PI, and maturation of hemagglutinin in MIN6 cells.
(A) MIN6 cells transfected with a phogrin-GFP expression vector were untreated or treated with olanzapine (50 µM) or risperidone (50 µM) for 4 hr and then subjected to pulse-chase experiment in the presence of olanzapine (50 µM) or risperidone (50 µM) to determine the rate of processing of phogrin-GFP. The amounts of full-length and cleaved phogrin-GFP were determined and are shown below with the amount of full-length phogrin-GFP at time 0 defined as 100% (n = 1). (B) MIN6 cells transfected with an A1PI expression vector were untreated or treated with olanzapine (50 µM) or risperidone (50 µM) for 4 hr and then subjected to pulse-chase experiment in the presence of olanzapine (50 µM) or risperidone (50 µM) to determine the rate of secretion of A1PI. The amounts of intracellular and extracellular A1PI were determined and are shown below with the amount of intracellular A1AP at time 0, defined as 100% (n = 3). (C) (D) MIN6 cells transfected with an A1PI expression vector were untreated (C) or treated with olanzapine (50 µM) for 14 hr (D). Fixed and permeabilized cells were analyzed by immunofluorescence using anti-A1PI and anti-KDEL antibodies. Bars: 5 μm. (E) MIN6 cells transfected with a hemagglutinin expression vector were untreated or treated with olanzapine (50 µM) or risperidone (50 µM) for 4 hr and then subjected to pulse-chase experiment to determine the rate of maturation of hemagglutinin. The immunoprecipitates were digested with endoglycosidae H and then analyzed by reducing SDS-PAGE and autoradiography. The amounts of high-mannose type and complex type hemagglutinin were determined and are shown below, with the amounts of high-mannose type hemagglutinin at time 0 defined as 100% (n = 1).
Immunofluorescence analysis using #8138 revealed that proinsulin mainly localized to perinuclear compartments, which were distinct from the ER reacted with the anti-calnexin antibody (Figure 7A) but overlapped with the localization of phogrin-GFP (Figure 7B), suggesting that they were included in immature or mature secretory granules.

Effect of olanzapine on localization of proinsulin and phogrin-GFP in MIN6 cells (Bars: 5 μm).
(A) MIN6 cells were analyzed by immunofluorescence using anti-insulin #8138 and anti-calnexin antibodies. (B) MIN6 cells transfected with a phogrin-GFP expression vector were analyzed by immunofluorescence using anti-insulin #8138 and anti-GFP antibodies. (C) – (F) MIN6 cells treated with (C) olanzapine (50 µM), (D) olanzapine (10 µM), (E) risperidone (50 µM), or (F) risperidone (10 µM) for 14 hr were analyzed by immunofluorescence using anti-insulin #8138 and anti-calnexin antibodies. (G) (H) MIN6 cells transfected with a phogrin-GFP expression vector were (G) untreated or (H) treated with olanzapine (50 µM) for 14 hr, and then analyzed by immunofluorescence using anti-GFP and anti-calnexin antibodies.
We were surprised to find that proinsulin colocalized with calnexin in MIN6 cells treated with 50 μM olanzapine (Figure 7C) and that this shift in localization was detected in MIN6 cells treated with 10 μM olanzapine (Figure 7D) but not in MIN6 cells treated with 50 μM or 10 μM risperidone (Figure 7E and F). In contrast, the localization of phogrin-GFP (Figure 7G and H) or insulin (Figure 8A and B) was not altered by treatment with 50 μM olanzapine. These results suggest that olanzapine specifically affects the quality of proinsulin during its localization in the ER.

Effect of olanzapine on localization of insulin as well as solubility and oligomerization of proinsulin in MIN6 cells.
MIN6 cells untreated (A) or treated with olanzapine (50 µM) (B) for 14 hr were analyzed by immunofluorescence using anti-insulin I2018 antibody. Bars: 5 μm. (C) MIN6 cells treated with DMSO (control), olanzapine (10 or 50 μM) or risperidone (50 μM) for 14 hr were lysed in 1% NP40. After centrifugation at 14,000 rpm for 10 min, supernatant and precipitate were analyzed by immunoblotting using anti-insulin #8138 and anti-GAPDH antibodies. Eight times greater amounts were used to analyze precipitate than total and supernatant. (D)-(G) MIN6 cells untreated or treated with olanzapine (50 µM) for 4 hr were lysed with 1% NP40 buffer containing 10 mM NEM. (D) Cell lysates were analyzed by reducing and non-reducing SDS-PAGE followed by immunoblotting using #8138. (E) Cell lysates were subjected to immunoprecipitation using #8138, and then to negative staining after non-reducing SDS-PAGE. (F) Gels at the position of HMP-1 were excised and analyzed by mass spectrometry. The results are shown by the scatter plot of log2 of the median peak area from three independent experiments between untreated cells (X axis) and olanzapine-treated cells (Y axis). A 5.7-fold increase by olanzapine treatment was observed for Ins2 as shown in the red circle. (G) Intensities of Ins2-derived fragments in untreated and olanzapine-treated cells in each experiment are shown along with the fold-induction.
Induction of proinsulin misfolding in olanzapine-treated MIN6 cells
Treatment of MIN6 cells with the proteasome inhibitor MG132 alone increased the levels of intracellular and extracellular proinsulin (Figure 5C and D, left panels), indicating that a part of newly synthesized proinsulin is constitutively subjected to ERAD in MIN6 cells. Importantly, simultaneous treatment of MIN6 cells with olanzapine and MG132 markedly increased the level of intracellular proinsulin (Figure 5D, right top panel), indicating that proinsulin forced to be remain in the ER of olanzapine-treated cells was subjected to ERAD. Nonetheless, ERAD of retained proinsulin alone cannot explain olanzapine-induced blockage of proinsulin secretion, because proinsulin secretion was still blocked in MIN6 cells treated with both olanzapine and MG132 (Figure 5D, right bottom panel), in contrary to the case with control cells (Figure 5D, left bottom panel).
As ERAD deals with misfolded proteins, we examined the effect of olanzapine on the solubility of proinsulin by lysing MIN6 cells with 1% NP40 followed by centrifugation, and found that a small amount of proinsulin became insoluble in MIN6 cells after treatment with 10 μM and 50 μM olanzapine but not with 50 μM risperidone (Figure 8C). We next checked whether olanzapine affects the disulfide-bonded status of proinsulin using #8138, which unlike I2018 does not recognize mature proinsulin (see Figure 3E). By analyzing under non-reducing conditions of immunoprecipitates obtained with #8138 after pulse chase (Figure 4A), we found that an approximately 27 kDa form of proinsulin, designated as a high molecular weight form of proinsulin (HMP-1), and an approximately 15 kDa form of proinsulin, designated as HMP-2, were produced in cells treated with olanzapine (Figure 4B, lanes 8–14) but not with risperidone (lanes 29–35) compared with control cells (lanes 1–7) [see quantified Figure 4B (c)]. HMP-1 and HMP-2 detected with #8138 but not with I2018 were not secreted at all, suggesting that they were severely misfolded.
To identify the components of HMP-1 and HMP-2, we conducted mass spectrometric analysis. To this end, olanzapine-untreated or -treated cells were lysed in buffer containing 10 mM N-ethylmaleimide (NEM) to maintain existing disulfide bonds. Subsequent non-reducing SDS-PAGE and immunoblotting revealed that proinsulin and HMP-2 somehow became undetected with #8138 (Figure 8D). Therefore, we were able to purify only HMP-1 by immunoprecipitation (Figure 8E). Mass spectrometric analysis of HMP-1 revealed that only insulin 2-derived fragments were enriched upon olanzapine treatment (Figure 8F and G, and Supplementary file 1), suggesting that HMP-2 and HMP-1 represent a proinsulin dimer and trimer, respectively, with aberrant intermolecular disulfide bonds.
To clarify how olanzapine induces the formation of aberrant proinsulin oligomers, we considered the possibility that olanzapine acts on certain oxidoreductases in the ER to inhibit their activities (Jang et al., 2019; Okumura et al., 2014), and therefore tested whether olanzapine directly binds to the purified enzymes using isothermal titration calorimetry (ITC), which sensitively detects heat generation or absorption upon ligand-substrate binding. However, results were negative for PDI, ERp46, ERp57, ERp72, and P5 (Figure 8—figure supplement 1A). We were also unable to precisely analyze direct interactions between olanzapine and proinsulin by ITC, because reduced proinsulin (Figure 8—figure supplement 1B) was rapidly and severely aggregated, producing significant heat exchange. Thus, direct targets of olanzapine remain to be determined.
ERAD of misfolded proinsulin in olanzapine-treated MIN6 cells and mouse islets
To determine whether olanzapine treatment indeed induces the production of aberrant high molecular weight forms of proinsulin and how quickly it does so, MIN6 cells pulse-labeled for 20 min were chased with the addition of olanzapine (Figure 9A). Analysis under non-reducing conditions of immunoprecipitates obtained with #8138 showed that most radioactivity was recovered as proinsulin in untreated cells (0 min), and secretion of proinsulin was inhibited in cells treated with olanzapine, as expected (Figure 9B). Importantly, the levels of HMP-1 and HMP-2 rapidly and markedly increased after treatment with olanzapine (Figure 9B).

Effect of the proteasome inhibitor MG132 on the levels of proinsulin, HMP-1, and HMP-2 in olanzapine-treated MIN6 cells.
(A) Schemes of the experiments shown in (B). (B) MIN6 cells pulse-labeled for 20 min were chased for the indicated periods with or without 50 µM olanzapine to determine changes in the levels of proinsulin (P), HMP-1 (1), and HMP-2 (2) in cells and medium as in Figure 4B using #8138 and non-reducing SDS-PAGE. The intensity of intracellular proinsulin at time 0 was defined as 100% (n = 3). (C) Schemes of the experiments shown in (D). (D) MIN6 cells untreated, treated with olanzapine (50 µM), or olanzapine (50 µM) and MG132 (30 µM) were analyzed as in Figure 4B to determine changes in the levels of proinsulin (P), HMP-1 (1), and HMP-2 (2) in cells and medium using #8138 and reducing and non-reducing SDS-PAGE. The intensity of intracellular proinsulin at time 0 was defined as 100% (n = 3).
To correlate the production of HMP-1 and HMP-2 with the ERAD of proinsulin, we added olanzapine together with or without MG132 at the start of starvation and then conducted pulse-chase experiments (Figure 9C), because we found that treatment with olanzapine produced maximal amounts of HMP-1 and HMP-2 within 40 min (Figure 9B). Analysis under reducing conditions of immunoprecipitates obtained with #8138 showed that the results obtained in Figure 9D (upper part) were consistent with those shown in Figure 5D for the level of intracellular and extracellular proinsulin. Critically, analysis under non-reducing conditions of immunoprecipitates obtained with #8138 revealed that MG132 stabilized proinsulin monomer, HMP-1, and HMP-2 in olanzapine-treated MIN6 cells (Figure 9D, lower part). These results strongly suggest that proinsulin monomer, HMP-1, and HMP-2 with aberrant disulfide bonds underwent ERAD (see Figure 10G).

Effect of olanzapine on proinsulin oligomerization in mouse islets.
(A)-(C) Isolated mouse islets were untreated or treated with the indicated concentration of olanzapine, GSK2656157 (GSK, 2 μM) or risperidone (10 μM) for the indicated period and analyzed by immunoblotting using anti-insulin #8138, anti-PDI and anti-GAPDH antibodies after reducing and non-reducing SDS-PAGE. $ denotes aggregated proinsulin. (D) Isolated mouse islets were treated or untreated with MG132 (30 μM) for 1 hr, then treated with olanzapine (50 μM) for the indicated period, and analyzed by immunoblotting using anti-insulin #8138 and anti-GAPDH antibodies after reducing SDS-PAGE. (E) (F) Islets were isolated from mice the indicated week after daily oral administration of the indicated dose of olanzapine, and analyzed by immunoblotting using anti-insulin #8138 and anti-GAPDH antibodies after reducing and non-reducing SDS-PAGE. (G) Model for olanzapine-induced β-cell dysfunction (see text).
We finally examined whether olanzapine induces misfolding of proinsulin at the mouse level. Non-reducing SDS-PAGE analysis followed by immunoblotting showed that HMP-1 and even higher molecular weight forms of proinsulin were produced when isolated mouse islets were incubated for 4 h with 50 μM olanzapine (Figure 10A), for 20 h with 5 μM and 10 μM olanzapine (Figure 10B) but not with 10 μM risperidone (Figure 10C) without affecting the molecular mass of PDI, an abundant ER-resident oxidoreductase (Figure 10B). Reducing SDS-PAGE analysis followed by immunoblotting showed that the level of proinsulin was decreased 4 h after incubation with 50 μM olanzapine (Figure 10A) and that this decrease was blocked by the addition of 30 μM MG132 from 1 h earlier (total 5 h) (Figure 10D). Of note, the level of proinsulin was markedly decreased 20 h after incubation with 5 μM and 10 μM olanzapine (Figure 10B).
Furthermore, non-reducing SDS-PAGE analysis followed by immunoblotting showed that HMP-1 and even higher molecular weight forms of proinsulin started to be detected in mouse islets isolated 1 week after daily oral administration of 3 mg/kg/day and 10 mg/kg/day olanzapine (Figure 10E), and were clearly detected in mouse islets isolated 2 weeks after daily oral administration of 10 mg/kg/day olanzapine (Figure 10E), and 5 weeks (Figure 10F) and 6 weeks (data not shown) after daily oral administration of 3 mg/kg/day olanzapine. Reducing SDS-PAGE analysis followed by immunoblotting showed that the level of proinsulin was decreased in mouse islets 5 weeks after daily oral administration of 3 mg/kg/day olanzapine (Figure 10F), although there was no significant change in the levels of blood glucose or plasma insulin at this stage (data not shown). These phenomena were not due to obesity because we observed no significant difference in body weight between control mice and mice after daily oral administration of 3 mg/kg/day olanzapine for 5 or 6 weeks (data not shown), but rather due to olanzapine-induced misfolding of proinsulin in pancreatic β cells.
Discussion
Here we identified a novel mechanism that accounts for olanzapine-induced atypical development of diabetes. For this purpose, we used the mouse pancreatic β−cell line MIN6 to show that olanzapine produced aberrantly disulfide-bonded proinsulin (Figure 4) and caused retention of misfolded proinsulin in the ER (Figure 7) which then underwent ERAD (Figure 9). Inhibition of insulin secretion as well as proinsulin secretion (Figures 1C and 5B) and retention of misfolded proinsulin in the ER (Figure 7D) was observed in MIN6 cells treated with 10 μM olanzapine, which is comparable with concentrations of olanzapine in patients sera (0.016–0.24 µM) and in postmortem serum (0.032–16 µM) (Robertson and McMullin, 2000), as well as with the observation that olanzapine concentrations in tissues are 4- to 46-fold higher than those in the plasma of rats (Aravagiri et al., 1999). It should be noted that olanzapine-induced apoptosis was not previously observed in MIN6 cells or in the hamster pancreatic β−cell line HIT-T15 treated with 10 μM olanzapine (Ozasa et al., 2013), indicating that olanzapine-induced misfolding of proinsulin is an early event which evokes ER stress in pancreatic β cells.
Importantly, olanzapine-induced misfolding of proinsulin, which is targeted to ERAD, also occurred when mouse islets were treated with olanzapine (Figure 10A–D). Furthermore, misfolding of proinsulin was induced in mouse islets after daily oral administration of 3 mg/kg/day olanzapine (Figure 10E and F). Patients usually take 10–20 mg olanzapine a day (0.17–0.33 mg/kg assuming 60 kg as body weight), which may correspond to 2.0–4.0 mg/kg in mice, if the difference in body surface areas is considered (multiply by 12.3) (Nair and Jacob, 2016). This mechanism can explain why certain patients rapidly develop diabetes as well as diabetic ketoacidosis after receiving olanzapine, without gaining weight, and why such patients recover when olanzapine is discontinued.
In this connection, Peter Arvan and colleagues recently showed that aberrantly disulfide-bonded proinsulin dimers and higher ladder complexes (trimer, tetramer, pentamer, ---) exist in rat pancreatic β-cell-derived INS1E cells as well as in mouse and human islets, and that the levels of these misfolded forms of proinsulin were markedly increased and instead the level of insulin was markedly decreased in islets of leptin receptor mutant LepRdb/db mice, a mouse model of diabetes, compared with islets of wild-type mice, before the onset of diabetes. They therefore proposed that proinsulin misfolding is an early event in the progression to type 2 diabetes (Arunagiri et al., 2019). The levels of these misfolded forms of proinsulin were dramatically increased after treatment of INS1E cells and islets of mouse and human with 2 μM GSK2656157, a potent PERK inhibitor (Atkins et al., 2013), for 20 hr and overnight, respectively, but not for 5 hr (Arunagiri et al., 2019), supporting the importance of the PERK branch of the UPR for maintenance of homeostasis of β cells, as described in the Introduction.
We confirmed the much more profound misfolding of proinsulin after treatment of mouse islets for 20 hr with 2 μM GSK2656157 than with 10 μM olanzapine, which was observed even under reducing conditions (Figure 10B; depicted by $, and data not shown). Interestingly, however, production of HMPs was observed after treatment of mouse islets for 20 hr with 10 μM olanzapine but not with 2 μM GSK2656157 (Figure 10B), and a ladder of HMPs bigger than HMP-1 was observed more clearly in mouse islets (Figure 10) than in MIN6 cells (Figure 9). This rapidity and broad extensiveness of olanzapine’s action on the folding status of proinsulin in mouse islets suggests that β-cell dysfunction may infrequently dominate and diabetes might develop without weight gain or insulin resistance in the minority of patients with potentially lower catabolism of olanzapine or by other unidentified factors. For example, it is known that olanzapine is catabolized by the action of cytochrome p450 (CPY) 1A2 and polymorphic CPY2D6 in the liver and that the induction of CPY1A2 by smoking significantly diminishes the concentration of olanzapine in plasma (Carrillo et al., 2003); as hospitalized patients cannot smoke, the concentration of olanzapine may be elevated in plasma and possibly in β cells of ex-smokers. The clearance of olanzapine also appears to vary by race, sex and age (Meibohm et al., 2002).
Correct formation of three intramolecular disulfide bonds is essential for insulin folding and activity (Haataja et al., 2016; Chang et al., 2003), which may be difficult even in unstressed cells. A part of newly synthesized proinsulin is therefore constitutively degraded by the proteasome (Figure 5D). We show that treatment with olanzapine markedly inhibited maturation and secretion of proinsulin, and instead induced aberrant disulfide bond-formation during the folding of proinsulin, leading to the formation of HMP-1 and HMP-2 (Figure 4), although we could not detect direct interaction between olanzapine and oxidoreductases in the ER by ITC (Figure 8—figure supplement 1).
Proinsulin in Akita mice with the Ins2 C96Y mutation fails to form the correct disulfide bonds, and is degraded by ERAD (He et al., 2015; Ron, 2002). To avoid unnecessary degradation of correctly folded proinsulin, the ER nucleotide exchange factor Grp170 distinguishes misfolded proinsulin from correctly structured proinsulin for disposal (Cunningham et al., 2017). Similarly, olanzapine-induced HMP-1 and HMP-2 may be recognized by Grp170 for targeting to ERAD as well. Proinsulin is not a glycoprotein and is degraded via the non-glycoprotein ERAD pathway, which can process severely misfolded glycoproteins as well (Ninagawa et al., 2015).
In conclusion, the mechanism identified here that mediates olanzapine-induced β-cell dysfunction should be considered, along with weight gain, in mitigating adverse side effects when patients with schizophrenia are prescribed olanzapine.
Materials and methods
Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Cell line (Mus musculus) | Insulinoma | Miyazaki et al., 1990 | MIN6 | The cell line has been authenticated and tested negative for mycoplasma. |
Recombinant DNA reagent | p3xFlag-CMV-14 | Sigma-Aldrich | ||
Recombinant DNA reagent | pcDNA3.1(+) | ThermoFisher | ||
Recombinant DNA reagent | Phogrin-GFP | Saito et al., 2011 | ||
Recombinant DNA reagent | A1PI | Ninagawa et al., 2015 | ||
Recombinant DNA reagent | HA | Gething and Sambrook, 1982 | ||
Antibody | Anti-insulin (Mouse monoclonal) | Cell signaling | Cat#: #8138 | WB (1:1000), IP (1:400), IF (1:100) |
Antibody | Anti-insulin (Mouse monoclonal) | Sigma-Aldrich | Cat#: I2018 | WB (1:1000), IP (1:400) |
Antibody | Anti-GFP (Mouse monoclonal) | Roche | Cat#: 11814460001 | IP (1:400), IF (1:100) |
Antibody | Anti-calnexin (Rabbit polyclonal) | Enzo Life Sciences | Cat#: ADI-SPA-865 | WB (1:1000), IF (1:100) |
Antibody | Anti-PDI (Rabbit polyclonal) | Enzo Life Sciences | Cat#: ADI-SPA-890 | WB (1:1000) |
Antibody | Anti-A1AT (Rabbit polyclonal) | Dako | Cat#: A0012 | IP (1:400), IF (1:100) |
Antibody | Anti-GAPDH (Rabbit polyclonal) | Trevigen | Cat#: 2275-PC-100 | WB (1:1000) |
Antibody | Anti-HA (Rabbit polyclonal) | Recenttec | Cat#: R4-TP1411100 | IP (1:400) |
Antibody | Anti-KDEL (Mouse monoclonal) | MBL | Cat#: M181-3 | IF (1:1000) |
Statistics
Statistical analysis was conducted using Student's t-test, with probability expressed as *p<0.05 and **p<0.01 for all figures.
Cell culture and transfection
Request a detailed protocolMIN6 cells (Miyazaki et al., 1990) were cultured in Dulbecco’s modified Eagle’s medium (DMEM containing 4.5 g/liter glucose) supplemented with 2 mM L-glutamine, 10% fetal bovine serum, and antibiotics (50 U/ml penicillin and 50 μg/ml streptomycin) at 37°C in a humidified atmosphere containing 5% CO2/95% air. Transfection was performed as described previously (Ninagawa et al., 2015) using Polyethyleneimine max (Polysciences) to replace Lipofectamine 2000 (Invitrogen). MIN6 cells were transfected 1 day after seeding, and 2 days later, pulse-chase experiments or immunofluorescence analysis were conducted.
Reagents and antibodies
Request a detailed protocolMG132 from the Peptide Institute; mastoparan from Wako; Z-VAD-fmk from Promega; and DMEM with or without 4.5 g/l (25 mM) glucose and protease inhibitor cocktail from Nacalai Tesque. Olanzapine and risperidone were purchased from Toronto Research Chemicals. Various antibodies were obtained as described in Key Resource Table.
Construction of plasmids
Request a detailed protocolRecombinant DNA techniques were performed according to standard procedures (Sambrook et al., 1989) and the integrity of all constructed plasmids was confirmed by extensive sequencing analyses. Mouse insulin cDNA was amplified from total RNA isolated from MIN6 cells using the primer pairs described in Supplementary file 2. These amplified fragments were inserted between the EcoRV and XhoI sites of pcDNA3.1 (Invitrogen). The plasmid to express mPhogrin-GFP (Saito et al., 2011), A1AT (Ninagawa et al., 2015) or hemagglutinin (Gething and Sambrook, 1982) was previously described.
ELISA
Request a detailed protocolApproximately 3 × 105 of MIN6 cells were plated in 24-well plates and cultured in DMEM containing 25 mM glucose for 3 days before pretreatment for 14 hr with olanzapine or risperidone, or for 4 days before pretreatment for 4 hr with olanzapine or risperidone. Cells were washed twice with Krebs-Ringer Bicarbonate (KRB) buffer (10 mM HEPES, approximately pH 7.0, containing 120 mM NaCl, 4.7 mM KCl, 2.5 mM CaCl2, 1.2 mM MgCl2, 1.2 mM KH2PO4, 25 mM NaHCO3, and 0.1% BSA) with 3 mM glucose (Minami et al., 2000). Cells were starved for glucose for 1 hr in KRB buffer with 3 mM glucose containing olanzapine or risperidone, washed twice with KRB buffer with 3 mM glucose, and stimulated for glucose for 1 hr in KRB buffer with 25 mM glucose containing olanzapine or risperidone, with or without mastoparan. The buffer was collected, and the cells were lysed with buffer A (50 mM Tris/HCl, pH 8.0, containing 1% NP-40, 150 mM NaCl, protease inhibitor cocktail, 20 μM MG132, and 2 μM Z-VAD-fmk). After brief centrifugation, the protein concentrations of the lysates were determined using the BCA protein assay reagent kit (Pierce). Insulin content was determined using an ELISA kit (Shibayagi) according to the manufacturer’s protocol. The amount of secreted insulin was normalized to the cellular protein content.
Immunoblotting
Request a detailed protocolImmunoblotting analysis was performed according to a standard procedure as previously described (Ninagawa et al., 2011). Chemiluminescence was generated using Western Blotting Luminol Reagent (Santa Cruz Biotechnology) and detected using an LAS-3000mini LuminoImage analyzer (Fuji Film). For detection of proinsulin and insulin, the pore size of PVDF membrane was changed from 0.45 to 0.2 µm (Amersham), and blocking and reaction with the anti-insulin monoclonal antibody was carried out in 5% BSA (Sigma-Aldrich) in PBS-0.1% Tween 20 buffer.
Pulse-chase experiment and in vitro translation
Request a detailed protocolPulse-chase experiments using 9.8 Mbq per dish of EASY-TAG EXPRESS Protein labeling mix [35S] (PerkinElmer) and subsequent immunoprecipitation using the anti-insulin monoclonal antibody and protein G-coupled Sepharose beads (GE Healthcare) were performed according to a published procedure (Ninagawa et al., 2014).
In vitro translation was performed using the TNT Quick Coupled Transcription/Translation Systems (Promega) and the EASY-TAG EXPRESS Protein labeling mix [35S] (PerkinElmer) according to the manufacturer’s instructions. Translated proteins were subjected to immunoprecipitation using the anti-insulin monoclonal antibody to separate them from 35S-methionine and 35S-cysteine. The immunoprecipitates were analyzed using SDS-PAGE (15 or 16% gel). Radiolabeled proteins were visualized using an FLA-3000G FluoroImage analyzer (Fuji Film).
Immunofluorescence assay
Request a detailed protocolFor immunofluorescence analysis, untransfected MIN6 cells or cells transfected using Polyethyleneimine max were washed with PBS and fixed with 4% paraformaldehyde phosphate buffer (Nacalai Tesque) on ice for 4.5 min. Fixed cells were washed with PBS and permeabilized by incubation on ice for 4.5 min in PBS containing 0.2% Triton X-100. After incubation in PBS containing 3% fetal bovine serum and the primary antibody for 2 hr at room temperature, cells were incubated with secondary antibodies labeled with Alexa Fluors 488, 568, or 633 (Thermo Fisher Scientific) for 1 hr at room temperature. Images were acquired at room temperature at 100 × magnification using a DM IRE2 and confocal software (both from Leica).
Mass spectrometric analysis
Request a detailed protocolNano-scale reversed-phase liquid chromatography coupled with tandem mass spectrometry (nanoLC/MS/MS) was performed with an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific), connected to a Thermo Ultimate 3000 RSLCnano pump and an HTC-PAL autosampler (CTC Analytics) equipped with a self-pulled analytical column (150 mm length ×100 μm i.d.) (Ishihama et al., 2002) packed with ReproSil-Pur C18-AQ materials (3 μm, Dr. Maisch GMBH). The mobile phases consisted of (A) 0.5% acetic acid and (B) 0.5% acetic acid and 80% acetonitrile. Peptides were eluted from the analytical column at a flow rate of 500 nl/min by altering the gradient: 5–10% B in 5 min, 10–40% B in 15 min, 40–100% B in 1 min and 100% for 4 min. The Orbitrap Fusion Lumos instrument was operated in the data-dependent mode with a full scan in the Orbitrap followed by MS/MS scans for 1.5 s using higher-energy collision dissociation (HCD). The applied voltage for ionization was 2.4 kV. The full scans were performed with a resolution of 120,000, a target value of 4 × 105 ions and a maximum injection time of 50 ms. The MS scan range was m/z 300–1500. The MS/MS scans were performed with a 15,000 resolution, a 5 × 104 target value and a 200 ms maximum injection time. Isolation window was set to 1.6 and normalized HCD collision energy was 30. Dynamic exclusion was applied for 20 s.
All raw datasets were analyzed and processed by MaxQuant (v1.6.2.3) (Cox and Mann, 2008). Default settings were employed. Search parameters included two missed cleavage sites and variable modifications such as methionine oxidation, protein N-terminal acetylation, cysteine carbamidomethyl and cysteine N-ethylmaleimide. The peptide mass tolerance was six ppm and the MS/MS tolerance was 20 ppm. Database search was performed with Andromeda (Cox et al., 2011) against the UniProt mouse database (downloaded on 2019–4) with common contaminants and enzyme sequences. False discovery rate (FDR) was set to 1% at peptide spectrum match (PSM) level and at protein level. For protein quantification, total peak area of the peptides was used, and median peak area was calculated for each protein from three independent experiments. To compare protein abundance between the non-treated and the olanzapine-treated cells (see Figure 8D), we considered proteins that were quantified in all samples and replicates.
ITC experiments
Request a detailed protocolITC measurements for the interaction between olanzapine and each PDI family protein were performed in buffer containing 50 mM HEPES-NaOH, pH 7.5, at 298 K and 750 r.p.m. For the preparation of olanzapine, 10 mg of olanzapine (32.0 mmol) was diluted in 500 µl of 50 mM HCl solution and freeze-dried using evaporator. 2.0 µl of the olanzapine solution (1.0 mM) was titrated into PDI family solutions (50 μM) at 180 s intervals after an initial 120 s delay. To minimize the effect of bubbles and imperfect solution filling of the syringe, the first titration was performed using 0.6 µl of solution in the syringe. The data were analyzed using MicroCal analysis (Malvern). The heats of dilution were subtracted from the raw binding data before analysis.
Expression and purification of human proinsulin
Request a detailed protocolRecombinant human proinsulin was expressed as inclusion bodies in Escherichia coli cells. Purification of proinsulin was carried out as previously described (Okumura et al., 2011). Briefly, inclusion bodies were treated with 100 mM Tris/HCl buffer, pH 8.0, containing 8 M urea and 10 mM DTT, and the solution was stood for 3 hr at 50°C. Reduced and denatured proinsulin was purified by RP-HPLC using Cosmosil 5C18-AR-II (4.6 mm I.D. ×250 mm, Nacalai Tesque) monitored at 220 nm. Molecular mass of purified proinsulin was calculated using ProteinProspectors (http://prospector.ucsf.edu/prospector/mshome.htm) and its identity was confirmed by MALDI-TOF/MS. Purified proinsulin was lyophilized at −80°C until used.
Animals
Male BALB/c mice (8 weeks old) were purchased from Shimizu Laboratory Supplies. All mouse experiments were conducted under pathogen-free conditions and in line with Institutional Animal Care protocols approved by Kyoto University (Q 19–68). Administration of vehicle or olanzapine was conducted using a reusable oral gavage needle once a day. Olanzapine was dissolved in 1% acetic acid in saline buffered with 1M NaOH to a pH >5.5 (McCormick et al., 2010).
Isolation of mouse pancreatic islets
Request a detailed protocolTo isolate islets from BALB/c mice, pancreas was inflated by injection of Hank’s Balanced Salt Solution (HBSS) containing 0.15 mg/ml collagenase P (Roche Diagnostics) via the common bile duct. Distended pancreas was then excised and incubated at 37°C for 18 min. After digested pancreas had dissociated, the tissue was washed with HBSS twice. The islets in the dissociated pancreatic tissue were purified on discontinuous gradients (1.110, 1.103, 1.096, and 1.070 g/ml) of OptiPrep (Axis-Shield) and ET Kyoto (ETK) solution (Otsuka Pharmaceutical). Isolated islets were cultured (37°C/5% CO2/95% air humidified atmosphere) in RPMI1640 medium supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin, and 100 μg/ml streptomycin.
Data availability
All data generated or analyzed during this study are included in the manuscript.
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Decision letter
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Elizabeth A MillerReviewing Editor; MRC Laboratory of Molecular Biology, United Kingdom
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Vivek MalhotraSenior Editor; The Barcelona Institute of Science and Technology, Spain
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Elizabeth A MillerReviewer; MRC Laboratory of Molecular Biology, United Kingdom
In the interests of transparency, eLife publishes the most substantive revision requests and the accompanying author responses.
Acceptance summary:
This analysis of the effect of the anti-psychotic olanzapine on misfolding and secretion of pro-insulin suggests a framework for understanding how such treatments might trigger rapid onset diabetes in patients. The authors elegantly show that olanzapine treatment alters the processing of pro-insulin such that an aggregation-prone intermediate accumulates, which is recalcitrant to secretion and thus accumulates in the endoplasmic reticulum. This has important implications for protein folding, secretion and ER stress in patients and provides a foundation for understanding the molecular basis by which this drug impacts protein folding.
Decision letter after peer review:
[Editors’ note: the authors submitted for reconsideration following the decision after peer review. What follows is the decision letter after the first round of review.]
Thank you for submitting your work entitled "Antipsychotic olanzapine targets proinsulin for endoplasmic reticulum-associated degradation by inducing its misfolding" for consideration by eLife. Your article has been reviewed by three peer reviewers, including Elizabeth A Miller as the Reviewing Editor and Reviewer #1, and the evaluation has been overseen by a Senior Editor.
Our decision has been reached after consultation between the reviewers. Based on these discussions and the individual reviews below, we regret to inform you that your work will not be considered further for publication in eLife.
Although there was enthusiasm for the research topic and the potential importance for human health, reviewers voiced significant concerns about the model systems. In particular, there was agreement that choice of the model is insufficient to support the claims with respect to disease/therapeutic relevance. Both reviewers 2 and 3 raised the problem of the lack of detection of insulin as a major concern, and had some suggestions regarding overcoming the different antibody specificity that might be helpful.
Reviewer #1:
Overall, this work provides strong evidence that OLA indirectly impairs insulin secretion by perturbing disulfide bond formation or rearrangement, thereby rendering pro-insulin trapped in the ER and subject to ERAD. This provides a nice explanation for physiological observations of diabetes induction in patients treated with this anti-psychotic medication and is therefore of general interest. However, whether the observed effects are direct or indirect remains to be clarified, which seems essential to warrant publication in eLife.
I have several specific concerns:
1) The quality of the immunofluorescence images is not particularly good.
2) A second disulfide-containing substrate should be investigated to show broad effects versus insulin-specific effects.
3) The activity of PDI should be assessed by proteomic analysis to test whether it is also forming mixed disulfides in a more promiscuous way, or if it is defective and thus the likely target of OLA.
Reviewer #2:
1) This paper wishes to address diabetes caused by olanzapine but there are no studies from drug-treated human or animal islets, which is a missed opportunity. Without islet work, the cell culture analysis really should be deeply insightful. In their previous publication they suggested that olanzapine blocks PERK action on eIF2α and "apoptosis was induced by olanzapine at the concentration of more than 50 μM" but neither PERK nor apoptosis appears in the current manuscript. In this manuscript, the authors show data suggesting that olanzapine does not bind proinsulin. So what is the primary drug target in β cells? It seems that most of what are measured in this manuscript are secondary effects of the drug without clarifying the primary effect. And, based on their previous paper, are the observed effects occurring in dying cells?
2) Figure 2B Figure legend says that the 26 ng of proinsulin run next to 2.9 micrograms of purified insulin, were subject to immunoblotting. The Results section concluded: "this antibody was more reactive with purified recombinant human proinsulin than purified recombinant human insulin". But no information is provided on whether the SDS-PAGE was performed under reducing or non-reducing conditions (the specific antibody also should be cited). It is essential that both conditions be shown in Figure 2B. Under reducing conditions, the band shown in the figure would not be insulin but one of the insulin chains split apart from insulin. If the immunoreactivity is much stronger with intact (non-reduced) insulin – then this might require significant re-interpretation of data within this manuscript – see below.
3) Figure 6C nonreducing gel shows three bands in all lanes in the region labeled as Proinsulin. If this anti-insulin antibody cannot see non-reduced insulin, then another immunoblot is required with an antibody that can recognize insulin. There is a simple question – do the untreated control β cells used in this study have any insulin? If not, is this a suitable β cell model? And how would the authors know whether olanzapine might affect the proteolytic processing from proinsulin to insulin? Conversely, if the antibody DOES see non-reduced insulin, are the authors entirely sure that the dark band in the control cells labeled Proinsulin (Figure 6C) is not actually Insulin? And if this is insulin, are we completely sure of which proteins are being recognized by immunofluorescence in the other figures?
4) The above discussion also raises the question of the physiological state of highly passaged Min6 cells. The authors cite a paper to suggest that their mouse β cells no longer express the mouse Ins1 gene, which is clearly abnormal. Can they not test the relative expression level of mouse Ins1 versus Ins2 mRNA (such as from qPCR with known quantities of Ins1 and Ins2 cDNA standards)? This might help to clarify what the various bands represent.
5) Figure 7C and 8 – I'm not sure why the authors conclude that HMI-1 is a major ERAD substrate? In Figure 8 at 40, 80, and even 160 minutes of chase in OLA-treated cells, the biggest difference between with and without MG132 is the proinsulin monomer. How can the authors know which form is the ERAD substrate?
6) A shorter pulse labeling would demonstrate whether there is a precursor-product relationship to the two proinsulin bands in Figure 2E, and immunoprecipitates with anti mature-insulin should be shown to determine whether insulin is getting stored intracellularly. It is troubling if the authors cannot distinguish a "processed form" (label on Figure 2) from "partially degraded proinsulin" (subsection “Inhibition of insulin secretion and retention of proinsulin in the ER in olanzapine-treated cells”). Is this an artifact of the cell line?
Reviewer #3:
The manuscript submitted to eLife by Mori and colleagues reports on a potential molecular mechanism that may lead to metabolic disease in individuals taking second generation antipsychotics, such as olanzapine. A previous conjecture was that the drug increased appetite as a result of its effect on serotonin receptors, ultimately leading to diabetes. However, data in this paper potentially indicate why a more immediate effect on diabetic syndrome is evident, uncover why olanzapine led to mild UPR/PERK activation in a prior study, and identify a putative underlying cause of this more rapid effect.
The authors first report that olanzapine shuts-down the levels of secreted insulin in a murine insulinoma cell model in a dose-dependent manner, and that the drug blunts the level of secreted proinsulin in a pulse-chase analysis. The production/secretion of two other trafficked proteins were unaffected. Olanzapine also altered the steady-state localization of proinsulin, leading to its residence in the ER instead of in secretory granules. Consistent with these data as well as the effect of the drug on the UPR, ERAD-targeting of proinsulin increased under the conditions used in these experiments. In addition, the use of non-reducing gels indicated that olanzapine administration led to the presence of a high molecular weight species that was primarily composed of proinsulin dimers and trimers, and it was the "HMI-1" form that was mostly degraded via the ERAD pathway.
While the experiments support the idea that olanzapine triggers the ERAD of proinsulin, resulting in lower circulating levels and potentially in metabolic syndrome, there are numerous caveats of the study as it currently stands. These are outlined below. In addition, only one cell line was used and the relevance to human physiology is questionable for several reasons, which are also outlined below. Finally, the mechanism by which olanzapine affects the ER redox balance and leads to proinsulin misfolding is completely mysterious. In short, the manuscript only hints at the major conclusions claimed by the authors.
Essential revisions:
1) What is the justification for using MIN6 cells, other than the use of a convenient antibody for pulse-chase studies (Figure 2C)? These cells actually lose glucose-sensitive insulin secretion over time. There is also no justification of why 25 mM glucose was administered in the MIN6 cell experiments. This concentration is well beyond what might be encountered in vivo after feeding.
2) Similar to the concerns raised above, drugs were apparently administered for 14 hours for steady state studies and 4 hours for the pulse-chase. What are the pharmacodynamics of the drug in the body, and in patients what is the actually effective concentration that is "seen" by β cells? How much of the drug is protein-bound? This knowledge is vital as only small percentages of drug are "free" in sera. Moreover, the authors claim that patient sera harbors 0.016 – 0.24 μm olanzapine, which is still far below the doses used here. They also claim that this may be negated by patients with "lower catabolism". Which cytP450s are used to metabolize the drug, and is there any evidence that this varies in patients? Overall, it is not clear whether the concentrations and conditions used in these experiments is physiologically relevant.
3) The inability to detect insulin production in the pulse-chase is problematic (Figure 2D). However, a pseudo pulse-chase could be performed by brief starvation, medium removal/wash, followed by a glucose pulse, addition of CHX, and subsequent ELISA for insulin in the medium. Therefore, insulin secretion could be directly detected over time, which is essential to show.
4) Does an amino acid starvation period in a pulse-chase assay impact insulin production/secretion?
5) The fluorescence images are of low quality (Figure 4) and the reticular network of the ER is not apparent.
6) Does combined administration of BFA and ± olanzapine also show increased ERAD of proinsulin? This would prevent the confounding variable of having to take secretion efficiency into account. More trivially, why are the data in Figure 5D presented first?
7) While the authors focus on the "HMI" species, the drug also increases the amount of protein >38 kDa. In fact, this species is more prevalent than the HMI forms and may reflect aggregated species (Figure 6C). Indeed, these species may or may not also be targeted for ERAD (molecular weight markers were not included in Figure 8B).
8) The authors state that the experiment in Figure 8 was "more reliable" under the conditions of "these conditions". If so, then the magnitude of the olanzapine effect on the ERAD of proinsulin (±MG132) in Figure 8B, bottom, is quite modest, especially given some of the caveats noted above.
[Editors’ note: further revisions were suggested prior to acceptance, as described below.]
Thank you for submitting your article "The antipsychotic olanzapine-induced misfolding and ERAD of proinsulin accounts for atypical development of diabetes" for consideration by eLife. Your article has been reviewed by three peer reviewers, including Elizabeth A Miller as the Reviewing Editor and Reviewer #1, and the evaluation has been overseen by Vivek Malhotra as the Senior Editor.
The reviewers have discussed the reviews with one another and the Reviewing Editor has drafted this decision to help you prepare a revised submission. After discussion among the reviewers and senior editor, we agreed that your study provides some thought-provoking new insight into the molecular mechanisms by which diabetes may arise rapidly after onset of patient treatment with olanzapine. That said, there was agreement among reviewers that some of the claims should be toned down, and you should state more clearly the limitations and caveats. Most importantly, the focus on ERAD (including in the title) was considered unwarranted. There was concern about the extent to which ERAD can account for the observed phenotypes, as well as the problem that MG132 treatment does not rescue secretion even if degradation is blocked. Therefore, ERAD is unlikely to explain insulin secretion defect, although reduced insulin secretion could partially explain the ERAD (this was the point of the BFA experiment suggested by Reviewer 3 in the original evaluation). ERAD should thus be appropriately de-emphasized as a driving mechanism. The other over-riding concern was about the concentrations of olanzapine that might accrue in patient cells. Of course, such measures are difficult to make, but by our estimates the effective concentration in your in vitro proinsulin folding experiment is at more than an order of magnitude higher than could possibly be achieved in patient tissue. Thus, the experiment shown in Figure 8C is particularly problematic, where a very high level of drug is required to see a quite subtle in vitro effect. The reviewers agreed that these data muddy the waters and should be removed. Thus, the ultimate conclusion is that although the mechanism of action remains unclear, your findings still provide insight into proinsulin misfolding as a potential side-effect of olanzapine treatment. You should be more careful in claiming a direct mechanism since that is not supported by the data shown.
We would like to draw your attention to changes in our revision policy that we have made in response to COVID-19 (https://elifesciences.org/articles/57162). Specifically, when editors judge that a submitted work as a whole belongs in eLife but that some conclusions require a modest amount of additional new data, as they do with your paper, we are asking that the manuscript be revised to either limit claims to those supported by data in hand, or to explicitly state that the relevant conclusions require additional supporting data.
Our expectation is that the authors will eventually carry out the additional experiments and report on how they affect the relevant conclusions either in a preprint on bioRxiv or medRxiv, or if appropriate, as a Research Advance in eLife, either of which would be linked to the original paper.
Reviewer #1:
In this revised version, the authors have added another antibody to establish the secretion of pro-insulin and insulin in their cell model, but many of the key experiments are all still only performed with the original antibody that doesn't recognise insulin. Thus, the primary concern (expressed by both other reviewers) that the precursor-product relationship cannot be established, has not been fully resolved. My interpretation of the data is that OLA treatment prevents the production of the P' ("processed proinsulin") form, but it was hard for me to know exactly what the P' protein corresponds to. One interpretation is that P' is aggregation prone (to yield the HMP products) and subject to ERAD. So, I suspect that most of the conclusions are correct but greater clarity is needed regarding the P' protein (what it is and how it changes with OLA treatment). This should therefore be quantified in all of the pulse-chase experiments presented (it is currently missing from many). The full precursor-product analysis requested in the original critique would go a long way to clarifying this.
Reviewer #2:
In the revised manuscript submitted to eLife, Mori and colleagues have added new data and models, and have addressed many of the comments that previously precluded publication. A better characterization of the antibodies, the inclusion of data with islets and other (unaffected) substrates, an effect with lower doses of olanzapine, and improved fluorescence images further support the authors' earlier conclusions. However, there is still the question of whether the effect of the drug is direct or indirect. An attempt to show interaction with PDIs, which was a long-shot, provided negative data (also see below), and even at the lower concentration of 10 uM, this value is still ~40x higher than serum levels in humans taking the drug. It is likely that the intracellular concentration in patients is even lower, given considerations regarding logP and the free vs bound population of the drug. Two reviewers also expressed doubt that ERAD targeting was a major contributor to reduced levels of pro-insulin. With the exception of a modest effect of MG132, there are no further supporting data. Therefore, the title and statements such as "indicating that a part of newly synthesized proinsulin is constitutively subjected to ERAD" are premature. In fact, it was surprising that a putative effect of MG132 in the islets was not examined, especially since "the level of proinsulin was markedly decreased 20 hours after incubation with 5 μm and 10 μm olanzapine". Moreover, a previous comment to determine whether enhanced ERAD targeting might be apparent when ER protein export is inhibited (for, example, with BFA or dominant negative SAR1) was completely misinterpreted by the authors in the rebuttal, who replied "We have not used BFA in this manuscript".
Essential revisions:
The experiments using ITC are problematic. 1 mM olanzapine was used, so any acquired data may be irrelevant to the results in cells or rodents. Furthermore, the in vitro effect of 1 mM on the refolding of denatured/reduced proinsulin lacks a key control. Does 1 mM risperidone also have the same, subtle effect?
In the experiments using mice in which there is an increase in the amount of HMP-1, there is similarly no control for olanzapine. Does risperidone, or an (inactive) olanzapine derivative, have the same effect?
Mass spec data (coverage, number of peptides) are not shown.
In subsection “Inhibition of insulin secretion in olanzapine-treated MIN6 cells”, risperidone is used without any mention of what this compound is or why it was chosen as a control.
In subsection “Retention of proinsulin in the ER in olanzapine-treated MIN6 cells”, a previous comment in the review with regard to AiPI was ignored: was it expected that the MIN6 cells express this protein? (Another reviewer commented on the prolonged passage of these cells and whether they truly reflect islet biology.) More mundane, the statement "Intracellular localization of A1PI was not affected…" is not clear. Do the authors mean that secretion was unaffected? Finally, what is the significance of using the anti-KDEL antibody. This is mentioned in the absence of any context.
The labeling of lanes/times in Figure 2E and Figure 3B needs to be fixed, and the labeling/spacing at the top of Figure 10A-C is odd.
In the Discussion section, the authors state, "olanzapine produced aberrantly disulfide-bonded proinsulin….leading to β-cell dysfunction". Are there any data to support this? The authors later state that the drug did not induce apoptosis, which appears to be in conflict with this earlier claim.
Reviewer #3:
The manuscript is much improved but Figure 8C and the conclusions associated with that panel are seriously flawed. Were it not for this panel I believe the paper could be acceptable for publication. There is also a small matter of Figure 9D.
Problems with Figure 8C:
1) 100 μm OLA kills β cells, but here the authors are adding 10 times more than this to try to get an effect in an in vitro folding assay. No conclusion can be reached from this experiment, which puts the text of the manuscript in this part at risk of not being supported by real data. The interpretation that the drug acts directly on proinsulin is almost certainly wrong – so why imply such a thing when it is totally unnecessary to the paper?
2) The starting material in this experiment seems to have a molecular mass that seems to be too big to be monomeric proinsulin.
Figure 9D:
Don't the authors want to comment on the fact that the monomeric proinsulin in the nonreducing gel is also protected by MG132? It is clearly shown on the gel and the quantification in the graph below. Why would the authors not make this point?
https://doi.org/10.7554/eLife.60970.sa1Author response
[Editors’ note: the authors resubmitted a revised version of the paper for consideration. What follows is the authors’ response to the first round of review.]
Reviewer #1:
Overall, this work provides strong evidence that OLA indirectly impairs insulin secretion by perturbing disulfide bond formation or rearrangement, thereby rendering pro-insulin trapped in the ER and subject to ERAD. This provides a nice explanation for physiological observations of diabetes induction in patients treated with this anti-psychotic medication and is therefore of general interest. However, whether the observed effects are direct or indirect remains to be clarified, which seems essential to warrant publication in eLife.
I have several specific concerns:
1) The quality of the immunofluorescence images is not particularly good.
It appears very difficult to obtain clear immunofluorescence image of β cells. Please note that the right figure shows immunofluorescence analysis of INS1 cells (Figure 1C of Arunagiri et al., 2019).
2) A second disulfide-containing substrate should be investigated to show broad effects versus insulin-specific effects.
We have shown that olanzapine also did not significantly affect the maturation of hemagglutinin from high mannose-type to complex type (Figure 5E), which requires correct disulfide bond formation for folding (Segal et al., 1992).
3) The activity of PDI should be assessed by proteomic analysis to test whether it is also forming mixed disulfides in a more promiscuous way, or if it is defective and thus the likely target of OLA.
We did not observe any changes in disulfide-bonded status of PDI in mouse islets after treatment with olanzapine (Figure 10B). Although we could not detect direct interaction between olanzapine and oxidoreductases in the ER by ITC (Figure 8A), we have found that olanzapine inhibited oxidative folding of purified proinsulin, leading to aberrant formation of intermolecular disulfide bonds (Figure 8C).
Reviewer #2:
1) This paper wishes to address diabetes caused by olanzapine but there are no studies from drug-treated human or animal islets, which is a missed opportunity. Without islet work, the cell culture analysis really should be deeply insightful.
We have shown that olanzapine-induced misfolding of proinsulin also occurred when mouse islets were treated with olanzapine (Figure 10A-C). Furthermore, misfolding of proinsulin was induced in mouse islets after daily oral administration of 3 mg/kg/day olanzapine (Figure 10D-E).
In their previous publication they suggested that olanzapine blocks PERK action on eIF2α and "apoptosis was induced by olanzapine at the concentration of more than 50 μM" but neither PERK nor apoptosis appears in the current manuscript. In this manuscript, the authors show data suggesting that olanzapine does not bind proinsulin. So what is the primary drug target in β cells? It seems that most of what are measured in this manuscript are secondary effects of the drug without clarifying the primary effect. And, based on their previous paper, are the observed effects occurring in dying cells?
We have found that olanzapine inhibited oxidative folding of purified proinsulin, leading to aberrant formation of intermolecular disulfide bonds (Figure 8C).
We have described that “Inhibition of insulin secretion as well as proinsulin secretion (Figures 1C and Figure 4B) and retention of misfolded proinsulin in the ER (Figure 6D) was observed in MIN6 cells treated with 10 µM olanzapine (Discussion section)” and that “It should be noted that olanzapine-induced apoptosis was not previously observed in MIN6 cells or in the hamster pancreatic β−cell line HIT-T15 treated with 10 µM olanzapine (Ozasa et al., 2013), indicating that olanzapine-induced misfolding of proinsulin is an early event which evokes ER stress in pancreatic β cells (Discussion section)".
2) Figure 2B Figure legend says that the 26 ng of proinsulin run next to 2.9 micrograms of purified insulin, were subject to immunoblotting. The Results section concluded: "this antibody was more reactive with purified recombinant human proinsulin than purified recombinant human insulin". But no information is provided on whether the SDS-PAGE was performed under reducing or non-reducing conditions (the specific antibody also should be cited). It is essential that both conditions be shown in Figure 2B. Under reducing conditions, the band shown in the figure would not be insulin but one of the insulin chains split apart from insulin. If the immunoreactivity is much stronger with intact (non-reduced) insulin – then this might require significant re-interpretation of data within this manuscript – see below.
We have improved Figure 2 and described that “It should be noted that #8138 was 800-fold more reactive with purified recombinant human proinsulin than purified recombinant human insulin (insulin B chain) under reducing conditions (Figure 2C) ( – subsection “Retention of proinsulin in the ER in olanzapine-treated MIN6 cells”)”.
3) Figure 6C nonreducing gel shows three bands in all lanes in the region labeled as Proinsulin. If this anti-insulin antibody cannot see non-reduced insulin, then another immunoblot is required with an antibody that can recognize insulin. There is a simple question – do the untreated control β cells used in this study have any insulin? If not, is this a suitable β cell model? And how would the authors know whether olanzapine might affect the proteolytic processing from proinsulin to insulin? Conversely, if the antibody DOES see non-reduced insulin, are the authors entirely sure that the dark band in the control cells labeled Proinsulin (Figure 6C) is not actually Insulin? And if this is insulin, are we completely sure of which proteins are being recognized by immunofluorescence in the other figures?
4) The above discussion also raises the question of the physiological state of highly passaged Min6 cells. The authors cite a paper to suggest that their mouse β cells no longer express the mouse Ins1 gene, which is clearly abnormal. Can they not test the relative expression level of mouse Ins1 versus Ins2 mRNA (such as from qPCR with known quantities of Ins1 and Ins2 cDNA standards)? This might help to clarify what the various bands represent.
We have employed mouse monoclonal antibody I2018 which immunoprecipitated mature proinsulin 2 and insulin 2 in addition to #8138 previously used, and have shown that MIN6 cells secrete both mature proinsulin and insulin (non-reducing conditions, Figure 2E).
5) Figure 7C and 8 – I'm not sure why the authors conclude that HMI-1 is a major ERAD substrate? In Figure 8 at 40, 80, and even 160 minutes of chase in OLA-treated cells, the biggest difference between with and without MG132 is the proinsulin monomer. How can the authors know which form is the ERAD substrate?
We have described that “Treatment of MIN6 cells with the proteasome inhibitor MG132 alone increased the levels of intracellular and extracellular proinsulin significantly (Figure 4C and 4D left), indicating that a part of newly synthesized proinsulin is constitutively subjected to ERAD in MIN6 cells (subsection “Induction of proinsulin misfolding in olanzapine-treated MIN6 cells”)” and that “Critically, analysis under non-reducing conditions of immunoprecipitates obtained with #8138 revealed that MG132 stabilized proinsulin, HMP-1, and HMP-2 in olanzapine-treated MIN6 cells (Figure 9D, lower part). These results strongly suggest that HMP-1 and HMP-2 underwent ERAD (subsection “ERAD of misfolded proinsulin in olanzapine-treated MIN6 cells and mouse islets”)”.
6) A shorter pulse labeling would demonstrate whether there is a precursor-product relationship to the two proinsulin bands in Figure 2E, and immunoprecipitates with anti mature-insulin should be shown to determine whether insulin is getting stored intracellularly. It is troubling if the authors cannot distinguish a "processed form" (label on Figure 2) from "partially degraded proinsulin" (subsection “Inhibition of insulin secretion and retention of proinsulin in the ER in olanzapine-treated cells”). Is this an artifact of the cell line?
We have shown that proinsulin (P) was accumulated and instead processed proinsulin (P’), mature prinsulin and insulin were decreased in olanzapine-treated MIN6 cells (Figure 3), indicating that olanzapine blocked maturation of proinsulin and accordingly inhibited secretion of insulin (subsection “Retention of proinsulin in the ER in olanzapine-treated MIN6 cells”).
Reviewer #3:
[…]
Essential revisions:
1) What is the justification for using MIN6 cells, other than the use of a convenient antibody for pulse-chase studies (Figure 2C)? These cells actually lose glucose-sensitive insulin secretion over time. There is also no justification of why 25 mM glucose was administered in the MIN6 cell experiments. This concentration is well beyond what might be encountered in vivo after feeding.
2) Similar to the concerns raised above, drugs were apparently administered for 14 hours for steady state studies and 4 hours for the pulse-chase. What are the pharmacodynamics of the drug in the body, and in patients what is the actually effective concentration that is "seen" by β cells? How much of the drug is protein-bound? This knowledge is vital as only small percentages of drug are "free" in sera. Moreover, the authors claim that patient sera harbors 0.016 – 0.24 μm olanzapine, which is still far below the doses used here.
We have shown that proinsulin misfolding occurred in MIN6 cells treated with 10 µM and 50 µM olanzapine (Figure 3 and Figure 4) and that HMP-1 and even higher molecular weight forms of proinsulin were produced when isolated mouse islets were incubated for 4 hours with 10 µM and 50 µM olanzapine (Figure 10A) and for 20 hours with 5 µM and 10 µM olanzapine (Figure 10B).
They also claim that this may be negated by patients with "lower catabolism". Which cytP450s are used to metabolize the drug, and is there any evidence that this varies in patients? Overall, it is not clear whether the concentrations and conditions used in these experiments is physiologically relevant.
We have described that “This rapidity and broad extensiveness of olanzapine’s action on the folding status of proinsulin in mouse islets suggests that β-cell dysfunction may infrequently dominate and diabetes might develop without weight gain or insulin resistance in the minority of patients with potentially lower catabolism of olanzapine or by other unidentified factors. For example, it is known that olanzapine is catabolized by the action of cytochrome p450 (CPY) 1A2 and polymorphic CPY2D6 in the liver and that the induction of CPY1A2 by smoking significantly diminishes the concentration of olanzapine in plasma (Carrillo et al., 2003); as hospitalized patients cannot smoke, the concentration of olanzapine may be elevated in plasma and possibly in β cells of ex-smokers. The clearance of olanzapine also appears to vary by race, sex and age (Meibohm et al., 2002)” (Discussion section).
3) The inability to detect insulin production in the pulse-chase is problematic (Figure 2D). However, a pseudo pulse-chase could be performed by brief starvation, medium removal/wash, followed by a glucose pulse, addition of CHX, and subsequent ELISA for insulin in the medium. Therefore, insulin secretion could be directly detected over time, which is essential to show.
4) Does an amino acid starvation period in a pulse-chase assay impact insulin production/secretion?
We have employed mouse monoclonal antibody I2018 which immunoprecipitated mature proinsulin 2 and insulin 2 in addition to #8138 previously used, and have shown that MIN6 cells secrete both mature proinsulin and insulin (non-reducing conditions, Figure 2E).
5) The fluorescence images are of low quality (Figure 4) and the reticular network of the ER is not apparent.
Please see our response to the comment 1 of the reviewer #1.
6) Does combined administration of BFA and ± olanzapine also show increased ERAD of proinsulin? This would prevent the confounding variable of having to take secretion efficiency into account. More trivially, why are the data in Figure 5D presented first?
We have not used BFA data in this manuscript.
7) While the authors focus on the "HMI" species, the drug also increases the amount of protein >38 kDa. In fact, this species is more prevalent than the HMI forms and may reflect aggregated species (Figure 6C). Indeed, these species may or may not also be targeted for ERAD (molecular weight markers were not included in Figure 8B).
In MIN6 cells, proteins >38 kDa were observed in even control cells, and HMP-1 and HMP-2 were induced by olanzapine treatment (Figure 9). In mouse islets, proteins >38 kDa were also induced by olanzapine treatment (Figure 10). Most importantly, we have succeeded in showing the decrease in the amount of proinsulin in mouse islets after treatment with olanzapine (Figure 10).
8) The authors state that the experiment in Figure 8 was "more reliable" under the conditions of "these conditions". If so, then the magnitude of the olanzapine effect on the ERAD of proinsulin (±MG132) in Figure 8B, bottom, is quite modest, especially given some of the caveats noted above.
We have replaced old Figure 8 with new Figure 9.
[Editors’ note: what follows is the authors’ response to the second round of review.]
The reviewers have discussed the reviews with one another and the Reviewing Editor has drafted this decision to help you prepare a revised submission. After discussion among the reviewers and senior editor, we agreed that your study provides some thought-provoking new insight into the molecular mechanisms by which diabetes may arise rapidly after onset of patient treatment with olanzapine. That said, there was agreement among reviewers that some of the claims should be toned down, and you should state more clearly the limitations and caveats. Most importantly, the focus on ERAD (including in the title) was considered unwarranted. There was concern about the extent to which ERAD can account for the observed phenotypes, as well as the problem that MG132 treatment does not rescue secretion even if degradation is blocked. Therefore, ERAD is unlikely to explain insulin secretion defect, although reduced insulin secretion could partially explain the ERAD (this was the point of the BFA experiment suggested by Reviewer 3 in the original evaluation). ERAD should thus be appropriately de-emphasized as a driving mechanism.
We have removed the word “ERAD” from the title and modified the main text based on our claim that olanzapine-induced misfolding of proinsulin and retention of misfolded proinsulin in the ER account for blockage of insulin secretion.
The other over-riding concern was about the concentrations of olanzapine that might accrue in patient cells. Of course, such measures are difficult to make, but by our estimates the effective concentration in your in vitro proinsulin folding experiment is at more than an order of magnitude higher than could possibly be achieved in patient tissue. Thus, the experiment shown in Figure 8C is particularly problematic, where a very high level of drug is required to see a quite subtle in vitro effect. The reviewers agreed that these data muddy the waters and should be removed. Thus, the ultimate conclusion is that although the mechanism of action remains unclear, your findings still provide insight into proinsulin misfolding as a potential side-effect of olanzapine treatment. You should be more careful in claiming a direct mechanism since that is not supported by the data shown.
We have removed the data in the previous Figure 8C and added that “although direct targets of olanzapine remain undetermined” to the Abstract and “Thus, direct targets of olanzapine remain to be determined” to the final paragraph of subsection “Induction of proinsulin misfolding in olanzapine-treated MIN6 cells”.
Reviewer #1:
In this revised version, the authors have added another antibody to establish the secretion of pro-insulin and insulin in their cell model, but many of the key experiments are all still only performed with the original antibody that doesn't recognise insulin. Thus, the primary concern (expressed by both other reviewers) that the precursor-product relationship cannot be established, has not been fully resolved. My interpretation of the data is that OLA treatment prevents the production of the P' ("processed proinsulin") form, but it was hard for me to know exactly what the P' protein corresponds to. One interpretation is that P' is aggregation prone (to yield the HMP products) and subject to ERAD. So, I suspect that most of the conclusions are correct but greater clarity is needed regarding the P' protein (what it is and how it changes with OLA treatment). This should therefore be quantified in all of the pulse-chase experiments presented (it is currently missing from many). The full precursor-product analysis requested in the original critique would go a long way to clarifying this.
The precursor-product relationship for proinsulin 2 and insulin 2 was already and clearly shown by pulse (20 minutes)-chase experiment using I2018 (Figure 3E and Figure 4B, lanes 15-18). We have added that “This also demonstrated the precursor-product relationship for proinsulin 2 and insulin 2 in MIN6 cells” (subsection “Retention of proinsulin in the ER in olanzapine-treated MIN6 cells”). We have also shown that olanzapine did not alter the localization of insulin (Figure 8A and 8B), allowing us to focus on the behavior of proinsulin in olanzapine-treated cells.
We have now shown the precursor-product relationship for proinsulin 2 and processed proinsulin 2 by conducting shorter pulse (3 min)-chase experiments using #8138 (Figure 3C).
We have noted that “The lower band was considered to be processed proinsulin 2, such as the B chain connected to the C peptide but not to the A chain as observed in isolated rat islets (Harding et al., 2012), or somewhat cleaved proinsulin, because only proinsulin 2 was detected after a shorter pulse (3minutes) and processed proinsulin 2 appeared after the 30-minutes chase (Figure 3C)” (subsection “Retention of proinsulin in the ER in olanzapine-treated MIN6 cells”).
We believe that processed proinsulin is unlikely to be aggregation prone (to yield the HMP products) because it was secreted (Figure 3B) and that it is likely to be produced around the Golgi apparatus because the level of processed proinsulin was markedly decreased by treatment with brefeldin A (Figure 3D). Our explanation for why OLA treatment prevents the production of processed proinsulin is that OLA treatment induced misfolding of proinsulun and retention of misfolded proinsulin in the ER.
We have noted that “Processed proinsulin 2 is likely to be produced after moving out from the ER, because its level was markedly decreased by treatment with brefeldin A, an inhibitor of anterograde transport from the ER to the Golgi apparatus (Figure 3D)” (subsection “Retention of proinsulin in the ER in olanzapine-treated MIN6 cells”).
Reviewer #2:
In the revised manuscript submitted to eLife, Mori and colleagues have added new data and models, and have addressed many of the comments that previously precluded publication. A better characterization of the antibodies, the inclusion of data with islets and other (unaffected) substrates, an effect with lower doses of olanzapine, and improved fluorescence images further support the authors' earlier conclusions. However, there is still the question of whether the effect of the drug is direct or indirect. An attempt to show interaction with PDIs, which was a long-shot, provided negative data (also see below), and even at the lower concentration of 10 uM, this value is still ~40x higher than serum levels in humans taking the drug. It is likely that the intracellular concentration in patients is even lower, given considerations regarding logP and the free vs bound population of the drug.
We would draw your attention to the finding that daily oral administration of olanzapine produced HMP-1 and even higher molecular weight forms of proinsulin in mouse islets (Figure 10E-F), albeit that we could not determine the concentration of olanzapine in serum.
Two reviewers also expressed doubt that ERAD targeting was a major contributor to reduced levels of pro-insulin. With the exception of a modest effect of MG132, there are no further supporting data. Therefore, the title and statements such as "indicating that a part of newly synthesized proinsulin is constitutively subjected to ERAD" are premature.
We have removed the word “ERAD” from the title.
We consider that the increase in the level of intracellular proinsulin in MIN6 cells treated with both olanzapine and MG132 over that in MIN6 cells treated with olanzapine alone is marked (Figure 5D, right top panel). Instead of toning this down, we have added that “Nonetheless, ERAD of retained proinsulin alone cannot explain olanzapine-induced blockage of proinsulin secretion, because proinsulin secretion was still blocked in MIN6 cells treated with both olanzapine and MG132 (Figure 5D, right bottom panel), in contrary to the case with control cells (Figure 5D, left bottom panel)” (subsection “Induction of proinsulin misfolding in olanzapine-treated MIN6 cells”).
In fact, it was surprising that a putative effect of MG132 in the islets was not examined, especially since "the level of proinsulin was markedly decreased 20 hours after incubation with 5 μm and 10 μm olanzapine".
We have now shown this effect and added that “Reducing SDS-PAGE analysis followed by immunoblotting showed that the level of proinsulin was decreased 4 hours after incubation with 50 µM olanzapine (Figure 10A) and that this decrease was blocked by the addition of 30 µM MG132 from 1 hour earlier (total 5 hours) (Figure 10D)” (subsection “ERAD of misfolded proinsulin in olanzapine-treated MIN6 cells and mouse islets”).
We have avoided the use of MG132 for 20 hours, considering its toxicity.
Moreover, a previous comment to determine whether enhanced ERAD targeting might be apparent when ER protein export is inhibited (for, example, with BFA or dominant negative SAR1) was completely misinterpreted by the authors in the rebuttal, who replied "We have not used BFA in this manuscript".
We apologize for our insufficient explanation. We tried to examine the effect of combined administration of brefeldin A and ± olanzapine on ERAD of proinsulin. To this end, we must treat MIN6 cells with (1) none, (2) olanzapine, (3) brefeldin A, (4) olanzapine and brefeldin, and (5) olanzapine, brefeldin A and MG132. During this experiment, we found that brefeldin A is quite toxic to MIN6 cells. In most cases, when MIN6 cells were treated with brefeldin A for longer periods, a lower amount of radioisotope was incorporated into proteins, as shown below. We thought that it is difficult to obtain meaningful results with this experiment.
Essential revisions:
The experiments using ITC are problematic. 1 mM olanzapine was used, so any acquired data may be irrelevant to the results in cells or rodents. Furthermore, the in vitro effect of 1 mM on the refolding of denatured/reduced proinsulin lacks a key control. Does 1 mM risperidone also have the same, subtle effect?
We have added that “although direct targets of olanzapine remain undetermined” to the Abstract and “Thus, direct targets of olanzapine remain to be determined” to subsection “Induction of proinsulin misfolding in olanzapine-treated MIN6 cells”. We have removed the data of the refolding of denatured/reduced proinsulin.
In the experiments using mice in which there is an increase in the amount of HMP-1, there is similarly no control for olanzapine. Does risperidone, or an (inactive) olanzapine derivative, have the same effect?
Risperidone did not increase the amount of HMP-1 over control (Figure 10C).
Mass spec data (coverage, number of peptides) are not shown.
The data are supplied as Table 1.
In subsection “Inhibition of insulin secretion in olanzapine-treated MIN6 cells”, risperidone is used without any mention of what this compound is or why it was chosen as a control.
We have described this as “However, it carries a higher risk of diabetes than other SGAs such as risperidone” (Introduction).
In subsection “Retention of proinsulin in the ER in olanzapine-treated MIN6 cells”, a previous comment in the review with regard to AiPI was ignored: was it expected that the MIN6 cells express this protein? (Another reviewer commented on the prolonged passage of these cells and whether they truly reflect islet biology.) More mundane, the statement "Intracellular localization of A1PI was not affected…" is not clear. Do the authors mean that secretion was unaffected? Finally, what is the significance of using the anti-KDEL antibody. This is mentioned in the absence of any context.
A1AT was expressed in MIN6 cells by transfection. We have changed this to “The antiKDEL antibody stained the ER by recognizing the major ER chaperones BiP/GRP78 and GRP94, and A1PI was widely distributed from the ER the Golgi apparatus before and after olanzapine treatment” (Figure 6C and 6D)” (subsection “Retention of proinsulin in the ER in olanzapine-treated MIN6 cells”).
The labeling of lanes/times in Figure 2E and 3B needs to be fixed, and the labeling/spacing at the top of Figure 10A-C is odd.
Please tell us more specifically what is odd and how it should be fixed.
In the Discussion section, the authors state, "olanzapine produced aberrantly disulfide-bonded proinsulin….leading to β-cell dysfunction". Are there any data to support this? The authors later state that the drug did not induce apoptosis, which appears to be in conflict with this earlier claim.
Dysfunction meant little insulin secretion, but we have removed “leading to b-cell dysfunction (Figure 10G)” (Discussion section).
Reviewer #3:
The manuscript is much improved but Figure 8C and the conclusions associated with that panel are seriously flawed. Were it not for this panel I believe the paper could be acceptable for publication. There is also a small matter of Figure 9D.
Problems with Figure 8C:
1) 100 μm OLA kills β cells, but here the authors are adding 10 times more than this to try to get an effect in an in vitro folding assay. No conclusion can be reached from this experiment, which puts the text of the manuscript in this part at risk of not being supported by real data. The interpretation that the drug acts directly on proinsulin is almost certainly wrong – so why imply such a thing when it is totally unnecessary to the paper?
We have removed the data in the previous Figure 8C.
2) The starting material in this experiment seems to have a molecular mass that seems to be too big to be monomeric proinsulin.
This was modified with AMS to stop the reaction, as was described previously in the legend.
Figure 9D:
Don't the authors want to comment on the fact that the monomeric proinsulin in the nonreducing gel is also protected by MG132? It is clearly shown on the gel and the quantification in the graph below. Why would the authors not make this point?
We have described this as “MG132 stabilized proinsulin monomer, HMP-1, and HMP-2 in olanzapine-treated MIN6 cells (Figure 9D, lower part). These results strongly suggest that proinsulin monomer, HMP-1, and HMP-2 with aberrant disulfide bonds underwent ERAD (see Figure 10G)” (subsection “ERAD of misfolded proinsulin in olanzapine-treated MIN6 cells and mouse islets”).
https://doi.org/10.7554/eLife.60970.sa2Article and author information
Author details
Funding
Ministry of Education, Culture, Sports, Science and Technology (18K06216)
- Satoshi Ninagawa
Ministry of Education, Culture, Sports, Science and Technology (19K06658)
- Tokiro Ishikawa
Ministry of Education, Culture, Sports, Science and Technology (17H01432)
- Kazutoshi Mori
Ministry of Education, Culture, Sports, Science and Technology (17H06419)
- Kazutoshi Mori
Gunma University (#18021 Joint Research Program of the Institute for Molecular and Cellular Regulation)
- Satoshi Ninagawa
- Seiji Torii
Takeda Science Foundation
- Masaki Okumura
Mochida Memorial Foundation for Medical and Pharmaceutical Research
- Masaki Okumura
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Acknowledgements
The authors declare no competing financial interests. We thank Kaoru Miyagawa for her technical and secretarial assistance, Dr. Akira Hattori (Kyoto University) and Ms. Masako Hirose (Malvern) for their help in the use of MicroCal Auto-iTC200, Dr. Jun-ichi Miyazaki for providing us MIN6 cells, Dr. Yuichi Tsuchiya (NAIST) and Dr. Masataka Kunii (Osaka University) for useful antibody information, and Ms. Nanae Fujimoto for her help in islet isolation. This work was financially supported in part by grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan (18K06216 to S N, 19K06658 to T I, 17H01432 and 17H06419 to K M), the Joint Research Program of the Institute for Molecular and Cellular Regulation, Gunma University (#18021 to S N and S T), the Takeda Science Foundation (to MO) and Mochida Memorial Foundation for Medical and Pharmaceutical Research (to MO).
Ethics
Animal experimentation: All mouse experiments were conducted under pathogen-free conditions and in line with Institutional Animal Care protocols approved by Kyoto University (Q 19-68).
Senior Editor
- Vivek Malhotra, The Barcelona Institute of Science and Technology, Spain
Reviewing Editor
- Elizabeth A Miller, MRC Laboratory of Molecular Biology, United Kingdom
Reviewer
- Elizabeth A Miller, MRC Laboratory of Molecular Biology, United Kingdom
Version history
- Received: July 14, 2020
- Accepted: October 22, 2020
- Version of Record published: November 17, 2020 (version 1)
Copyright
© 2020, Ninagawa et al.
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
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