A tightly regulated innate immune response to trypanosome infections is critical to strike a balance between parasite control and inflammation-associated pathology. In this study, we make use of the recently established Trypanosoma carassii infection model in larval zebrafish to study the early response of macrophages and neutrophils to trypanosome infections in vivo. We consistently identified high- and low-infected individuals and were able to simultaneously characterize their differential innate response. Not only did macrophage and neutrophil number and distribution differ between the two groups, but also macrophage morphology and activation state. Exclusive to high-infected zebrafish, was the occurrence of foamy macrophages characterized by a strong pro-inflammatory profile and potentially associated with an exacerbated immune response as well as susceptibility to the infection. To our knowledge this is the first report of the occurrence of foamy macrophages during an extracellular trypanosome infection.
All data generated or analyzed during this study are included in the manuscript and supporting files. Source data files have been provided for Figures 2, 4, 5, 6
- Eva Dóró
- Sem H Jacobs
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Animal experimentation: All animals were handled in accordance with good animal practice as defined by the European Union guidelines for handling of laboratory animals http://ec.europa.eu/environment/ chemicals/lab_animals/home_en.htm). Allanimal work at Wageningen University was approved by the local experimental animal committee (DEC number 2014095).
- Malcolm J McConville, The University of Melbourne, Australia
- Received: November 1, 2020
- Accepted: June 9, 2021
- Accepted Manuscript published: June 11, 2021 (version 1)
© 2021, Jacobs et al.
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