CTP and parS coordinate ParB partition complex dynamics and ParA-ATPase activation for ParABS-mediated DNA partitioning
ParABS partition systems, comprising the centromere-like DNA sequence parS, the parS-binding ParB-CTPase and the nucleoid-binding ParA-ATPase, ensure faithful segregation of bacterial chromosomes and low-copy-number plasmids. F-plasmid partition complexes containing ParBF and parSF move by generating and following a local concentration gradient of nucleoid-bound ParAF. However, the process through which ParBF activates ParAF-ATPase has not been defined. We studied CTP- and parSF-modulated ParAF-ParBF complex assembly, in which DNA-bound ParAF-ATP dimers are activated for ATP hydrolysis by interacting with two ParBF N-terminal domains. CTP or parSF enhances the ATPase rate without significantly accelerating ParAF-ParBF complex assembly. Together, parSF and CTP accelerate ParAF-ParBF assembly without further significant increase in ATPase rate. Magnetic-tweezers experiments showed that CTP promotes multiple ParBF loading onto parSF-containing DNA, generating condensed partition complex-like assemblies. We propose that ParBF in the partition complex adopts a conformation that enhances ParBF-ParBF and ParAF-ParBF interactions promoting efficient partitioning.
All data generated or analysed during this study are included in the manuscript and supporting files. Source data files have been provided for all relevant figures.
Article and author information
NIDDK/NIH (Intramural Research Fund)
- Kiyoshi Mizuuchi
NHLBI/NIH (Intramural Research Fund)
- Keir C Neuman
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
- Thomas Surrey, Centre for Genomic Regulation (CRG), Spain
- Received: December 10, 2020
- Preprint posted: January 25, 2021 (view preprint)
- Accepted: July 20, 2021
- Accepted Manuscript published: July 21, 2021 (version 1)
- Version of Record published: August 11, 2021 (version 2)
This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
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