Host-associated microbe PCR (hamPCR) enables convenient measurement of both microbial load and community composition
- Max Planck Institute for Developmental Biology, Germany
- University of Tübingen, Germany
Abstract
The ratio of microbial population size relative to the amount of host tissue, or 'microbial load', is a fundamental metric of colonization and infection, but it cannot be directly deduced from microbial amplicon data such as 16S rRNA gene counts. Because existing methods to determine load, such as serial dilution plating, quantitative PCR, and whole metagenome sequencing, add substantial cost and/or experimental burden, they are only rarely paired with amplicon sequencing. We introduce host-associated microbe PCR (hamPCR), a robust strategy to both quantify microbial load and describe interkingdom microbial community composition in a single amplicon library. We demonstrate its accuracy across multiple study systems, including nematodes and major crops, and further present a cost-saving technique to reduce host overrepresentation in the library prior to sequencing. Because hamPCR provides an accessible experimental solution to the well-known limitations and statistical challenges of compositional data, it has far-reaching potential in culture-independent microbiology.
Data availability
All data in this manuscript have been deposited in the European Nucleotide Archive (ENA) under the project number PRJEB38287. At https://www.ebi.ac.uk/ena.
Article and author information
Author details
Funding
Deutsche Forschungsgemeinschaft (SPP 2125 DECRyPT)
- Derek S Lundberg
- Pratchaya Pramoj Na Ayutthaya
- Detlef Weigel
Human Frontiers Science Program Long-term Fellowship (LT000565/2015-L)
- Derek S Lundberg
Max Planck Society
- Derek S Lundberg
- Pratchaya Pramoj Na Ayutthaya
- Gautam Shirsekar
- Wen-Sui Lo
- Detlef Weigel
Cluster of Excellence EXC2124 Controlling Microbes to Fight Infection (390838134)
- Derek S Lundberg
- Pratchaya Pramoj Na Ayutthaya
- Gautam Shirsekar
- Wen-Sui Lo
- Detlef Weigel
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2021, Lundberg et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Host-associated microbe PCR (hamPCR) enables convenient measurement of both microbial load and community composition
eLife 10:e66186.
https://doi.org/10.7554/eLife.66186
