Phases of cortical actomyosin dynamics coupled to the neuroblast polarity cycle
The Par complex dynamically polarizes to the apical cortex of asymmetrically dividing Drosophila neuroblasts where it directs fate determinant segregation. Previously we showed that apically directed cortical movements that polarize the Par complex require F-actin (Oon and Prehoda, 2019). Here we report the discovery of cortical actomyosin dynamics that begin in interphase when the Par complex is cytoplasmic but ultimately become tightly coupled to cortical Par dynamics. Interphase cortical actomyosin dynamics are unoriented and pulsatile but rapidly become sustained and apically-directed in early mitosis when the Par protein aPKC accumulates on the cortex. Apical actomyosin flows drive the coalescence of aPKC into an apical cap that is depolarized in anaphase when the flow reverses direction. Together with the previously characterized role of anaphase flows in specifying daughter cell size asymmetry, our results indicate that multiple phases of cortical actomyosin dynamics regulate asymmetric cell division.
All data generated or analysed during this study are included in the manuscript and supporting file
Article and author information
National Institutes of Health (GM127092)
- Kenneth E Prehoda
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
- Yukiko M Yamashita, Whitehead Institute/MIT, United States
- Preprint posted: January 15, 2021 (view preprint)
- Received: January 18, 2021
- Accepted: November 12, 2021
- Accepted Manuscript published: November 15, 2021 (version 1)
- Version of Record published: December 2, 2021 (version 2)
© 2021, Oon & Prehoda
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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