The metabotropic glutamate receptors (mGluRs) form a family of neuromodulatory G protein-coupled receptors that contain both a seven-helix transmembrane domain (TMD) and a large extracellular ligand-binding domain (LBD) which enables stable dimerization. While numerous studies have revealed variability across subtypes in the initial activation steps at the level of LBD dimers, an understanding of inter-TMD interaction and rearrangement remains limited. Here we use a combination of single molecule fluorescence, molecular dynamics, functional assays, and conformational sensors to reveal that distinct TMD assembly properties drive differences between mGluR subtypes. We uncover a variable region within transmembrane helix 4 (TM4) that contributes to homo- and heterodimerization in a subtype-specific manner and tunes orthosteric, allosteric and basal activation. We also confirm a critical role for a conserved inter-TM6 interface in stabilizing the active state during orthosteric or allosteric activation. Together this study shows that inter-TMD assembly and dynamic rearrangement drive mGluR function with distinct properties between subtypes.
We have provided source data files for all relevant figures.
- Joshua Levitz
- Jordana K Thibado
- Marta Filizola
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
- Janice L Robertson, Washington University in St Louis, United States
- Received: January 29, 2021
- Accepted: April 19, 2021
- Accepted Manuscript published: April 21, 2021 (version 1)
© 2021, Thibado et al.
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