Functional and structural segregation of overlapping helices in HIV-1
- University of California, San Francisco, United States
- Scribe Therapeutics, United States
Abstract
Overlapping coding regions balance selective forces between multiple genes. One possible division of nucleotide sequence is that the predominant selective force on a particular nucleotide can be attributed to just one gene. While this arrangement has been observed in regions in which one gene is structured and the other is disordered, we sought to explore how overlapping genes balance constraints when both protein products are structured over the same sequence. We use a combination of sequence analysis, functional assays and selection experiments to examine an overlapped region in HIV-1 that encodes helical regions in both Env and Rev. We find that functional segregation occurs even in this overlap, with each protein spacing its functional residues in a manner that allows a mutable non-binding face of one helix to encode important functional residues on a charged face in the other helix. Additionally, our experiments reveal novel and critical functional residues in Env and have implications for the therapeutic targeting of HIV-1.
Data availability
Sequencing data have been deposited in GEO under accession codes GSE179046Code is available on https://github.com/jferna10/EnvPaper. All graphs were generated inRstudio with associated files available on github and/or in Supplementary Tables.
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Functional and Structural Segregation of Overlapping Helices in HIV-1NCBI Gene Expression Omnibus,GSE179046.
Article and author information
Author details
Funding
National Institute of Allergy and Infectious Diseases (P50AI150476)
- Alan D Frankel
National Institute of General Medical Sciences (T32AI0605357)
- Maliheh Safari
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2022, Safari et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Functional and structural segregation of overlapping helices in HIV-1
eLife 11:e72482.
https://doi.org/10.7554/eLife.72482
