Characterization of the endogenous DAF-12 ligand and its use as an anthelmintic agent in Strongyloides stercoralis
Abstract
A prevalent feature of Strongyloides stercoralis is a life-long and potentially lethal infection that is due to the nematode parasite’s ability to autoinfect and, thereby, self-replicate within its host. Here, we investigated the role of the parasite’s nuclear receptor, Ss-DAF-12, in governing infection. We identified Δ7-DA as the endogenous Ss-DAF-12 ligand and elucidated the hormone’s biosynthetic pathway. Genetic loss of function of the ligand’s rate-limiting enzyme demonstrated that Δ7-DA synthesis is necessary for parasite reproduction, whereas its absence is required for the development of infectious larvae. Availability of the ligand permits Ss-DAF-12 to function as an on/off switch governing autoinfection, making it vulnerable to therapeutic intervention. In a preclinical model of hyperinfection, pharmacologic activation of DAF-12 suppressed autoinfection and markedly reduced lethality. Moreover, when Δ7-DA was administered with ivermectin, the current but limited drug of choice for treating strongyloidiasis, the combinatorial effects of the two drugs resulted in a near cure of the disease.
Editor's evaluation
This work reveals the pathway by which an important human parasite synthesizes a nuclear hormone receptor ligand critical for progression through its life cycle and demonstrates the potential therapeutic implications of perturbing this pathway. The experiments are insightfully and expertly conceived, designed and executed, and the data support the conclusions. This manuscript will be of general interest to parasitologists, nematode biologists, and those studying transcriptional regulatory networks governed by ligand-gated nuclear receptors.
https://doi.org/10.7554/eLife.73535.sa0Introduction
Strongyloidiasis is a neglected tropical disease caused by the nematode parasite, Strongyloides stercoralis. It is estimated to infect ~600 million people worldwide and is endemic in Africa, Asia, Latin America, and parts of the Caribbean, southern United States and Europe (Buonfrate et al., 2020). Because of the unique nature of its lifecycle, S. stercoralis infections are often life-long and up to 2.5% of these infections will progress to a hyperinfection syndrome that has a 90% mortality rate if untreated (Milder et al., 1981). Notably, such hyperinfections are often caused by administering glucocorticoids to otherwise asymptomatic patients (Milder et al., 1981). The lifecycle of S. stercoralis is atypical of most soil-transmitted helminths (Krolewiecki et al., 2013; Lok, 2007; Page et al., 2018; Figure 1). Infective third-stage larvae (L3i) are developmentally quiescent and reside in fecal-contaminated soil until they contact the host and penetrate the skin, a process that reactivates their development as L3+ larvae. In the host, the activated L3+ immediately begin feeding, and typically migrate to the lungs, enter the alimentary canal, and transit to the intestine where the stage four larvae (L4) mature into parasitic (P)-adult females (there are no parasitic males). The P-adult females reproduce asexually and generate rhabditiform, noninfectious stage one larvae (L1) progeny that can enter two alternate lifecycle routes, one in the external environment and one in the host. Postparasitic L1 (PP-L1) enter the external environment by leaving the host with the feces. Under conditions that mimic the host intestine (e.g., 37°C), most PP-L1 develop as females directly into infectious filariform L3i (Albarqi et al., 2016). However, under favorable conditions (e.g., 22 °C, high humidity) the majority of PP-L1 undergo a developmental switch and become free-living (FL) male and female larvae that eventually mature into FL-adults, which are morphologically distinct from parasitic females and feed on soil microbes. The FL-adults mate and produce post-free-living (PFL) larvae that are exclusively female and now definitively committed to become infective L3i larvae.
In addition to the conditions that produce L3i outside of the host, S. stercoralis has the ability to remain in the host and undergo continuous autoinfection (Figure 1). This is due to the presence of L1 larvae that develop directly into autoinfective third-stage larvae (L3a) within the intestine. Similar to their L3i counterparts, L3a infect the host, in this case by penetrating the intestinal wall and entering parenteral tissues as activated L3+ to complete the parasitic lifecycle. Autoinfection is unique to S. stercoralis and allows the parasite to persist in the host for decades as a latent, often asymptomatic infection. However, under certain host conditions such as immunosuppression by glucocorticoids, the autoinfection spirals out of control, dramatically increasing the parasite burden. The resulting hyperinfection leads to a breakdown of the intestinal mucosal barrier and dissemination of invasive larvae throughout the body accompanied by bacterial infection leading to sepsis that is responsible for the high mortality. The current treatment of choice is the nematode-selective chloride channel activator ivermectin, which is effective at controlling acute infections, but is limited by its inability to eradicate the persistent autoinfective larvae (Krolewiecki et al., 2013; Repetto et al., 2018). Perhaps for this reason, the efficacy of ivermectin in preventing fatality due to the hyperinfection and disseminated forms of the disease is as low as 50% (Buonfrate et al., 2013), and cases of ivermectin resistance are now being reported in other nematode parasites (Prichard, 2007). The current increased prevalence of strongyloidiasis has stimulated efforts to include increased dosing of ivermectin through mass drug administration (Bisoffi et al., 2013), which in the long-term could accelerate ivermectin resistance in S. stercoralis. Of further immediate concern, the risk of hyperinfection has risen dramatically with the use of glucocorticoids to treat inflammatory diseases, particularly SARS-CoV-2 (Moloo, 2020). For these reasons, there is an urgent need to develop new therapeutic strategies for treating strongyloidiasis (Krolewiecki et al., 2013; Moloo, 2020).
The lifecycle of S. stercoralis has similarities to the free-living nematode, C. elegans. The developmentally arrested L3i stage of S. stercoralis is analogous to the L3 dauer (d) stage of C. elegans. In C. elegans, L3d development is governed by DAF-12 (Antebi et al., 1998; Antebi et al., 2000), a nematode-specific nuclear receptor that is conserved in parasitic species (Ayoade et al., 2020; Long et al., 2020; Ma et al., 2019; Motola et al., 2006; Ogawa et al., 2009; Wang et al., 2017; Wang et al., 2009). In favorable environments, developing larvae synthesize the endogenous C. elegans (Ce)-DAF-12 ligands called dafachronic acids (Mahanti et al., 2014; Motola et al., 2006). The most potent and abundant of these ligands is Δ7-dafachronic acid (Δ7-DA), which induces a transcriptional program that governs metabolism and reproductive growth to mature adults (Bethke et al., 2009; Hammell et al., 2009; Wang et al., 2015). The final and rate-limiting step of Δ7-DA synthesis is catalyzed by DAF-9, a cytochrome P450 (Gerisch and Antebi, 2004; Jia et al., 2002; Motola et al., 2006). In unfavorable environments, Δ7-DA is not produced and unliganded Ce-DAF-12 functions as a transcriptional repressor that arrests growth as L3d larvae in a process known as dauer diapause. When favorable conditions return, Δ7-DA synthesis resumes, DAF-12 is transcriptionally active, and worms exit L3 dauer and continue reproductive development. Our previous work demonstrated a similar requirement for the transactivation of the orthologous S. stercoralis (Ss)-DAF-12 receptor in governing L3i (Albarqi et al., 2016; Wang et al., 2009) and the essentiality of the receptor in this process (Cheong et al., 2021). Although the endogenous Ss-DAF-12 ligand was unknown, we demonstrated that pharmacologic administration of Δ7-DA to PP-L1 or PFL-L1 larvae commits them to FL reproductive development, even under conditions that would normally commit them to become L3i (Albarqi et al., 2016; Wang et al., 2009), and institutes a transcriptomic profile in L3i similar to L3+ (Stoltzfus et al., 2014). In addition, knockout of Ss-DAF-12 prevents the formation of L3i larvae (Cheong et al., 2021), and in a mouse model of hyperinfection, exogenous Δ7-DA treatment lowers the worm burden in the intestine (Patton et al., 2018).
Despite these findings, an understanding of the DAF-12 signaling pathway during parasitism and importantly whether targeting the Ss-DAF-12 receptor is a viable, curative strategy for treating strongylodiasis is lacking. In this report, we identified the endogenous Ss-DAF-12 ligand, characterized its biosynthetic pathway, and demonstrated how its regulation governs the parasite’s lifecycle. Finally, using a relevant preclinical animal model, we show that pharmacological activation of Ss-DAF-12 overcomes the deficiencies of ivermectin, and that when used in combination with ivermectin, provides a combinatorial therapeutic response that may be curative.
Results
Δ7-DA is the endogenous ligand for DAF-12 in S. stercoralis
Our previous work had demonstrated Δ7-DA is able to bind and activate Ss-DAF-12 (Wang et al., 2009). However, whether Δ7-DA or a related molecule is the endogenous S. stercoralis ligand is unknown. Indeed, the finding that the ligand binding pocket of Ss-DAF-12 is divergent from that of Ce-DAF-12 (Wang et al., 2009) and the observation that S. stercoralis lacks an obvious ortholog of DAF-9 (the only known DA-synthesizing enzyme found in C. elegans) suggested that the Ss-DAF-12 ligand would likewise be divergent. To determine the identity of S. stercoralis DAF-12 ligands, we employed an unbiased, activity-based biochemical purification strategy (Figure 2—figure supplement 1). In a control experiment using a DA-deficient strain of C. elegans supplemented with a known quantity of DA, we demonstrated this strategy effectively recovered 70% of the ligand (Figure 2—figure supplement 1B). Following this strategy, we fractionated lipids extracted from the FL-L3 larvae of S. stercoralis, which are analogous to the L3 stage of C. elegans where DA levels are highest (Li et al., 2013; Motola et al., 2006). Among the 70 high-performance liquid chromatography (HPLC) fractions, Ss-DAF-12 ligand activity was detected only in fraction 23 in a cell-based reporter assay and this activity was dose dependent (Figure 2A). Remarkably, fraction 23 coeluted with dafachronic acids (Figure 2—figure supplement 1B), suggesting that the ligand is a related molecule.
To test whether the identified ligand activity is a dafachronic acid, we utilized a method incorporating ultra-performance liquid chromatography coupled with mass spectrometry (UPLC–MS) that specifically detects all known DA ligands (e.g., Δ4-DA, Δ7-DA, and Δ1,7-DA). Surprisingly, comparing the UPLC–MS data to DA standards (Table 1) revealed the presence of Δ7-DA (Figure 2B) but no other congeners, such as Δ4-DA and Δ1,7-DA (Figure 2—figure supplement 2). The predicted endogenous concentration of Δ7-DA in S. stercoralis FL-L3 is ~200 nM, which is well above the concentration needed to fully activate Ss-DAF-12 (Figure 2B, inset). Taken together, these data demonstrate that Δ7-DA is present in S. stercoralis and acts as an endogenous DAF-12 ligand.
Δ7-DA levels correlate with reproductive development in S. stercoralis lifecycle
In C. elegans, DAF-12 ligands promote reproductive development (Bethke et al., 2009; Hammell et al., 2009; Motola et al., 2006) and adulthood longevity (Gerisch et al., 2007; Yamawaki et al., 2010). To examine where in the S. stercoralis lifecycle Δ7-DA is present, lipid extracts from each developmental stage were analyzed by UPLC–MS (Figure 3). In the postparasitic environment under conditions that promote FL development, Δ7-DA was present in FL-L1/L2 and was significantly more abundant in the FL-L3 stage as the larvae progress toward reproductive maturity (Figure 3A–C). Similarly, Δ7-DA was detected in L3+ that have just infected the host and begun their reproductive development (Figure 3A–C). These findings are consistent with what is observed in C. elegans undergoing normal reproductive development (Li et al., 2013; Motola et al., 2006). A unique aspect of S. stercoralis is that after one FL generation outside of the host, PFL larvae are fated to growth arrest at L3i. In concordance with the hypothesis that developmental arrest is due to the absence of a DAF-12 ligand, Δ7-DA was undetectable in FL-adults, PFL-L1, and L3i larvae (Figure 3A–C).
The intestinal (Int)-L1-L3a larvae that were the direct progeny of parasitic adults in hyperinfected gerbils also had virtually no Δ7-DA (Figure 3A–C). These larvae that remain within the intestine are committed to become infectious as L3a, similar to their PFL counterparts that become L3i (Lok, 2007; Viney and Lok, 2015). By comparison, in the external postparasitic environment under temperature conditions that mimic the host intestine, the PP-L1 are also fated to become infectious as L3i (Figure 3C; Albarqi et al., 2016). The absence of Δ7-DA in these two stages (L3i and L3a) is similar to the developmental diapause observed in C. elegans, in which larvae arrest at the L3d stage in the absence of the DAF-12 ligand (Motola et al., 2006). Taken together with our previous work demonstrating that parasites lacking Ss-DAF-12 fail to respond to Δ7-DA and cannot enter or exit the L3i stage (Cheong et al., 2021), we conclude that liganded DAF-12 in S. stercoralis is required for reproductive development, while unliganded DAF-12 is requisite for the production of infectious L3i and L3a larvae.
Another intriguing finding was that the highest concentration of Δ7-DA occurred in parasitic adults, as opposed to the essentially undetectable levels in FL adults (Figure 3B). This difference in Δ7-DA levels correlated with the markedly different lifespans of the two adult populations. Parasitic adults live for up to a year or more in the host, whereas FL adults survive for only a few days in soil (Lok, 2007; Viney and Lok, 2015). Given that Δ7-DA is known to extend lifespan of adult C. elegans (Gerisch et al., 2007; Yamawaki et al., 2010), this finding suggests that Δ7-DA may play a similar role in extending the lifespan of parasitic adults in S. stercoralis.
Identification of the Δ7-DA biosynthetic pathway in S. stercoralis
In C. elegans, DAF-12 ligands are synthesized from dietary cholesterol by a cascade of enzymes including a Rieske oxygenase (DAF-36), a short-chain hydroxysteroid dehydrogenase (DHS-16), and a cytochrome P450 (CYP; DAF-9) that catalyzes the final and rate-limiting step in dafachronic acid synthesis (Figure 4A; Motola et al., 2006; Rottiers et al., 2006; Wollam et al., 2012; Wollam et al., 2011). Bioinformatic analysis of the S. stercoralis genome revealed single homologs for both DAF-36 and DHS-16 (Stoltzfus et al., 2012), which share sequence identity with their C. elegans counterparts. In contrast, there are 26 CYPs in S. stercoralis, but none of these share predictive sequence identity with DAF-9 (~36% at the best) (Stoltzfus et al., 2012). The lack of an obvious DAF-9 ortholog suggested the possibility that Δ7-DA might be acquired exogenously by S. stercoralis. To rule out this possibility, we first determined whether S. stercoralis synthesize Δ7-DA from dietary cholesterol in vivo. FL PP-L1 were cultured on a diet where the natural cholesterol isotope (largely 12C-cholesterol) was supplemented with 13C-labeled cholesterol. After 24 hr, we detected significant levels of 13C-labeled Δ7-DA in the FL-L3 (Figure 4B). These results demonstrated that Δ7-DA is generated from dietary cholesterol in S. stercoralis and that the parasite has all of the necessary enzymatic machinery to perform the synthesis.
To characterize the biosynthetic enzymes, we employed a Sf9 cell expression system that we used to identify the C. elegans counterparts of these proteins (Motola et al., 2006; Rottiers et al., 2006; Wollam et al., 2012; Wollam et al., 2011). To avoid potential interference from any endogenous substrates in Sf9 cells, we utilized 2H-isotope-labeled substrates to assay the activity of the candidate S. stercoralis orthologs, Ss-DAF-36 (SSTP_0000037900) and Ss-SCDH-16 (SSTP_0001031100, the homolog of Ce-DHS-16). Sf9 microsomes expressing Ss-DAF-36 readily converted cholesterol to 7-dehydrocholesterol (Figure 4C) with an efficacy comparable to the C. elegans enzyme (Figure 4D). Similarly, Ss-SCDH-16 catalyzed the conversion of lathosterol to lathosterone like Ce-DHS-16 (Figure 4E, F). These findings demonstrated that S. stercoralis has functional orthologs of DAF-36 and DHS-16.
The inability to predict the parasite’s DAF-9 ortholog from our bioinformatic analysis prompted us to perform an unbiased screen to identify the enzyme from among all 26 S. stercoralis CYPs. Each of the 26 CYP enzymes was expressed in Sf9 cells (Figure 4—figure supplement 1) and assayed by UPLC–MS for their ability to synthesize Δ7-DA from lathosterone. Unambiguously, only one of the 26 CYPs, Ss-CYP22a9 (SSTP_0001032100) synthesized Δ7-DA at levels comparable to Ce-DAF-9 (Figure 4G). These results demonstrated that Ss-CYP22a9 is the DAF-9 isoenzyme in S. stercoralis.
Δ7-DA synthesis is required for reproductive development in S. stercoralis
In C. elegans, favorable environmental cues induce DAF-9 expression and DAF-12 ligand synthesis through a cGMP signaling pathway, leading to reproductive development (Hu, 2007). Likewise in S. stercoralis, favorable host conditions act through a similar pathway to stimulate feeding, a sign of reactivated development in L3i (Stoltzfus et al., 2014). To study the role of Δ7-DA synthesis in parasite reproductive development, we first asked whether Δ7-DA is made in response to activation of the cGMP pathway in L3i. To that end, we treated L3i with 8-Br-cGMP, which mimics the presence of the host environment by activating the cGMP signaling pathway, and then tested endogenous Δ7-DA levels in the L3i larvae. In the presence of 8-Br-cGMP, we found that endogenous Δ7-DA was increased progressively over a 3-day period to levels (~100 nM) that would saturate Ss-DAF-12 occupancy (Figure 5). Furthermore, this stimulation of Δ7-DA synthesis correlated exactly with the expression of Ss-cyp22a9 (Figure 5—figure supplement 1A) and was abolished by cotreatment with ketoconazole, a broad-spectrum CYP inhibitor (Figure 5). These results highlight the importance of Δ7-DA synthesis in the reactivation of L3i larval development.
We next tested the requirement of Ss-CYP22a9 for Δ7-DA synthesis and parasite development in vivo. For these experiments, a CRISPR-mediated, homology-directed gene knockout strategy was used to insert a GFP expression cassette into the Ss-cyp22a9 gene locus through homologous repair (Figure 5—figure supplement 1B, C). This strategy allowed us to enrich for Ss-cyp22a9 knockout worms by selecting GFP-positive L3i. Single worm genotyping confirmed the presence of at least one disrupted Ss-cyp22a9 allele in >60% of the GPF-positive L3i (Figure 5B). To evaluate the phenotype due to loss of Ss-CYP22a9, we assayed cGMP-induced feeding behavior in L3i, which as noted above is an early marker of the transition to reproductive development (Lok, 2007; Stoltzfus et al., 2014; Viney and Lok, 2015). In both wild-type larvae and CRISPR-control larvae in which a gene unrelated to development (Ss_unc-22) was targeted, 8-Br-cGMP strongly induced the feeding behavior as expected (Figure 5C). In contrast, in Ss-cyp22a9 knockout larvae, cGMP-induced feeding behavior was severely impaired (Figure 5C). The residual cGMP-induced response is likely due to the presence of an incomplete penetrance of the knockout in all worms. The loss of this feeding behavior in Ss-cyp22a9-deficient larvae was rescued completely by treatment with exogenous Δ7-DA, further demonstrating the importance of DAF-12 activation as a requirement for parasite development (Figure 5C). To confirm the enzymatic function of Ss-CYP22a9, we also analyzed the production of Δ7-DA in Ss-cyp22a9 knockout L3i larvae. For this experiment, we targeted the Ss-cyp22a9 gene using nonhomologous end joining CRISPR with the same sgRNA as in Figure 5C and Figure 5—figure supplement 1C. This CRISPR method permitted the generation of larger numbers of Ss-cyp22a9 knockouts, which were necessary to detect endogenous Δ7-DA levels. Consistent with the results of the CYP inhibitor, disrupting the Ss-cyp22a9 gene abolished the Δ7-DA induction by cGMP (Figure 5D). Taken together, these results demonstrated the crucial role of Ss-CYP22a9 in Δ7-DA synthesis and highlight the importance of Δ7-DA synthesis in the development of infectious larvae in S. stercoralis.
Δ7-DA suppresses parasitic burden in uncomplicated strongyloidiasis
In S. stercoralis, uncomplicated infections are frequently asymptomatic but can last for years due to the persistence of L3a worms that escape the host immune system and continually reinfect the host at a low level. However, when the host is immune suppressed and the intestinal barrier is compromised, autoinfection accelerates out of control as more and more L3a worms invade the host, leading to a lethal hyperinfection. The results above suggested that targeting the DAF-12 pathway might provide a novel therapeutic opportunity for treating this disease. This strategy is based on the idea that like their L3i counterparts in the environment, the lack of Δ7-DA is required for intestinal larvae to become infectious L3a (Figure 3C). Thus, we postulated that pharmacological activation of DAF-12 in intestinal larvae should block autoinfection by preventing the formation of filariform L3a larvae as it does with L3i in the environment (Albarqi et al., 2016; Wang et al., 2009). We first asked whether Δ7-DA can successfully treat the uncomplicated, latent form of the disease. To that end, we employed a well-established gerbil model that mimics both uncomplicated and hyperinfective strongyloidiasis found in humans (Kerlin et al., 1995; Nolan et al., 1993).
For the uncomplicated model, we infected gerbils with 1000 L3i and after 21 days began administering vehicle or Δ7-DA orally in their drinking water, which delivers the compound directly into the gut where the intestinal larvae reside. We then monitored the parasite burden of the intestine by counting the number of fecal larvae. Compared to vehicle, treatment with Δ7-DA for 14 days dramatically reduced fecal larval output by ~90% (312 ± 103 larvae per gram feces for vehicle vs 32 ± 6 for DA, q < 0.03; Figure 6). In contrast, the numbers of parasitic adults, which produce intestinal larvae, were not significantly changed by Δ7-DA treatment (Figure 6B). Because of the time-frame of this experiment and the fact that autoinfection is minimal in the uncomplicated model (Nolan et al., 1993), reinfection of the host by L3a larvae is negligible, demonstrating that these adult parasites were derived from the L3i of the initial infection. This explains why the numbers of adults (in contrast to the intestinal larvae) were not substantially reduced by Δ7-DA treatment. These results are also consistent with our observation that intestinal larvae lack Δ7-DA (Figure 3), which is essential for L3a development, thereby supporting the hypothesis that Δ7-DA specifically targets the intestinal larval stages of the lifecycle. We conclude that Δ7-DA treatment impedes the development of intestinal larvae and thus blocks transmission in uncomplicated infections where conventional treatments are only partially effective.
Δ7-DA improves survival of a lethal strongyloidiasis hyperinfection
Based on the finding that Δ7-DA disrupts intestinal larval development (Figure 6A), we next asked whether the DAF-12 ligand would be effective at treating the lethal form of strongyloidiasis. For this experiment, beginning at the time of infection with L3i, gerbils were administered the glucocorticoid, methylprednisolone, on a weekly basis (Nolan et al., 1993). Similar to what is observed in immunocompromised humans, this results in the rapid progression of disease to a disseminated hyperinfection that is lethal to all of the animals, with >90% of them dying within 40 days (Figure 7, black line). In contrast, when a matched group of these animals was treated with Δ7-DA starting at 10 days after the hyperinfection was initiated, there was an impressive 70% survival rate that lasted for at least 90 days at which point we arbitrarily terminated the experiment (Figure 7A, blue line). Autopsies of these animals showed that hyperinfection resulted in a massive increase in the number of viable intestinal parasites (>104) in vehicle-treated gerbils, which was reduced substantially in Δ7-DA-treated animals (Figure 7A-D). Notably, the number of autoinfected L3+ larvae in parenteral tissues, which drive the resulting pathogenesis, was reduced by 90% (and in some cases was undetectable) in Δ7-DA-treated animals compared to vehicle-treated animals (Figure 7C). These results are consistent with what was observed in the uncomplicated infection and supports the conclusion that Δ7-DA treatment impedes the development of L3a. Compared to vehicle-treated gerbils with uncomplicated infections (Figure 6B), we observed a >10-fold increase in adult parasites in hyperinfected vehicle-treated gerbils (Figure 7D). However, unlike in the uncomplicated infection, there was a significant decrease in adult parasites in the Δ7-DA-treated gerbils that had a hyperinfection (50% vs. the vehicle treated, Figure 7D). This difference is because in a hyperinfection the parasitic adults are largely derived from the expanding L3a population, whereas in an uncomplicated infection the number of adults that were present matured directly from the L3i larvae used for the initial infection. Overall, these findings demonstrated that Δ7-DA treatment is effective in treating a lethal S. stercoralis hyperinfection.
Δ7-DA and ivermectin act cooperatively to treat strongyloidiasis hyperinfection
As the current front-line drug for treating strongyloidiasis, ivermectin is effective at removing adult S. stercoralis parasites (Krolewiecki et al., 2013; Repetto et al., 2018). However, in hyperinfected patients ivermectin treatment is only 50–60% effective at preventing death (Buonfrate et al., 2013). This high mortality is due in part to the relatively low efficacy of ivermectin at killing the persistent autoinfective L3a larvae. Given that Δ7-DA specifically targets the L3a population, we hypothesized that the combination of Δ7-DA and ivermectin would act cooperatively to treat a strongyloidiasis hyperinfection. To test this, we first established an ivermectin treatment model in hyperinfected gerbils that mimics the efficacy observed in humans (Figure 7—figure supplement 1). In this model, 300 μg/kg ivermectin delivered i.p. acutely cleared fecal larva output in ~40% of the hyperinfected gerbils within 7 days, resulting in ~25% survival rate for at least 90 days (Figure 7A, red line). As expected, ivermectin was as effective as Δ7-DA in removing adult parasites from animals undergoing hyperinfection (Figure 7D), but in contrast to Δ7-DA, ivermectin was ineffective at decreasing either the intestinal or L3+ larval populations (Figure 7B, C). Next, we treated hyperinfected gerbils with both Δ7-DA and ivermectin. We found that the combination of both agents improved the survival rate significantly over either agent alone (>75%, Figure 7A) and reduced the parasite burden of all life stages (Figure 7B–D). Remarkably, the combination of Δ7-DA and ivermectin completely eliminated the parasites from all the animals that survived, essentially curing them of the disease (Figure 7E). In contrast, when treated with ivermectin or Δ7-DA alone, ≤10% were parasite-free even among those animals that survived (Figure 7E). Taken together, these results demonstrated that Δ7-DA and ivermectin act synergistically in successfully eliminating S. stercoralis by targeting complementary stages of the lifecycle (Figure 8).
Discussion
The worldwide prevalence of S. stercoralis infections and the increased risk of deadly hyperinfection due to the broad use of glucocorticoids to treat inflammatory diseases underscores the urgent need for new therapeutic approaches to treat strongyloidiasis (Buonfrate et al., 2013; Repetto et al., 2018). The difficulty in treating the disease arises from a persistent autoinfection in which L3a are produced and continuously reinfect the host. Consequently, the parasite burden can become uncontrollably large when the immune system is compromised, leading to frequent lethality. Our previous studies have suggested one potential target of interest is the nuclear receptor, DAF-12 (Patton et al., 2018; Wang et al., 2017; Wang et al., 2009), which is required for the transition into and out of the L3i stage of the parasite’s lifecycle (Cheong et al., 2021). Here, we addressed two key unanswered questions: what is the nature and role of the Ss-DAF-12 ligand, and is targeting the Ss-DAF-12 pathway a viable therapeutic strategy? In this report, we identified Δ7-DA as the endogenous ligand of Ss-DAF-12 and showed that the regulation of its biosynthesis is a requirement for both reproductive growth and the development of infectious stage larvae. Similar to the free-living nematode, C. elegans, we found the presence of Δ7-DA is a requisite feature for S. stercoralis FL and parasitic larvae to mature into reproductive adults. In contrast, we discovered Δ7-DA is absent during the formation of L3a. Thus, like the analogous development of L3i in the environment, unliganded DAF-12 appears to be required for L3a formation in hosts. Taken together, these findings revealed a vulnerability in the lifecycle of S. stercoralis and suggested two new therapeutic strategies: either blocking the synthesis of the ligand to prevent reproductive development or precociously activating DAF-12 in the intestine to prevent autoinfection.
To that end, we showed that knocking out the Δ7-DA biosynthetic enzyme prevents the initiation of reproductive development. Concomitantly, when gerbils infected with S. stercoralis were treated with Δ7-DA, the development of L3a was severely impaired, thereby suppressing the parasite’s autoinfection cycle in both uncomplicated and hyperinfection forms of strongyloidiasis. Strikingly, when Δ7-DA was combined with the front-line medication, ivermectin, there was a near complete remission of the hyperinfection that resulted in a substantial increase in survival (from 25% with ivermectin alone to 75% with the combination treatment). Notably, in all of the surviving animals receiving both Δ7-DA and ivermectin, no parasites from any stage were detected anywhere in the body, suggesting that the cotreatment resulted in a complete cure.
Advantages of DAF-12 as a therapeutic target
The synergistic response of Δ7-DA and ivermectin highlights the unique potential of targeting the DAF-12 signaling pathway and overcoming the limitations of using ivermectin alone. Mortality rates in ivermectin-treated patients with hyperinfection range as high as 50% (Buonfrate et al., 2013). As an inhibitor of muscular function, ivermectin is highly effective at killing adults and feeding larvae (Repetto et al., 2018), but less effective at killing the developmentally quiescent L3a larvae that continuously drive autoinfection. In contrast, Δ7-DA directly interferes with infectious L3a larval development by binding to unliganded DAF-12 and provoking an inappropriate developmental response inside the host that results in death of the larvae. In this way, the two drugs complement each other by targeting different stages of the lifecycle (Figure 8). Targeting the ligand binding properties of DAF-12 has other distinct advantages in that this nuclear receptor is nematode specific and mutants resistant to pharmacologic ligands are unlikely to develop, since such mutants would be expected to disrupt the endogenous function of the receptor and otherwise be lethal.
The conundrum of Δ7-DA as the Ss-DAF-12 ligand
An intriguing aspect of this study was the finding that Δ7-DA is the endogenous ligand for Ss-DAF-12. Although Δ7-DA was first identified as the endogenous DAF-12 ligand in C. elegans, its presence in S. stercoralis was unexpected. In comparison to its C. elegans homolog, the Ss-DAF-12 ligand binding domain shares only moderate sequence identity (42%) and exhibits a distinct pharmacologic profile in response to Δ7-DA compared to other species (Wang et al., 2009). The sequences of other regulatory proteins in the Ss-DAF-12 pathway also have diverged. For example, the cytochrome P450 (Ss-CYP22a9) that catalyzes the synthesis of Δ7-DA (Figure 4) and the ligand-dependent coactivator that Ss-DAF-12 requires for transactivation (Ss-DIP-1) (Cheong et al., 2021) are both unique to Strongyloides spp.
In spite of these differences, several lines of evidence indicate that Δ7-DA is the major, if not exclusive, DAF-12 ligand in S. stercoralis. First, no other ligand activities, including other types of dafachronic acids were detected. Second, the endogenous concentration of Δ7-DA in S. stercoralis (~200 nM) is sufficient to drive full activation of Ss-DAF-12, leaving other potential DAF-12 ligands redundant if they do exist. Finally, knocking out the daf-9 homolog, Ss-cyp22a9, which is required to synthesize Δ7-DA, prevents DAF-12-dependent activity in vivo, and that activity is rescued completely by adding back the ligand. Interestingly, Δ7-DA has been shown to activate DAF-12 homologs in other species (Ayoade et al., 2020; Long et al., 2020; Ma et al., 2019; Ogawa et al., 2009; Wang et al., 2009). Why the Δ7-DA hormone has been evolutionarily conserved, whereas other components of the pathway have not, remains unclear, but nevertheless highlights the importance of the ligand in governing nematode biology.
Therapeutic potential beyond S. stercoralis
The findings discussed above underscore the potential of developing agonists that would precociously activate unliganded Ss-DAF-12 and kill L3a larvae, a therapy that would be propitious and specific to S. stercoralis. However, the observation that liganded DAF-12 also is required at a completely different but essential stage of the lifecycle to promote reproductive growth raises the possibility of also targeting DAF-12 with an antagonist. Given that DAF-12 has been found in all nematode species surveyed to date (Ayoade et al., 2020; Long et al., 2020; Ma et al., 2019; Ogawa et al., 2009; Wang et al., 2009), antagonizing the receptor would be expected to prevent development of adult worms in all parasitic species, offering a unique, broad-spectrum approach for treating most, if not all, nematode parasitisms.
In addition to targeting the receptor, the identification of Ss-CYP22a9 as the Δ7-DA-synthesizing enzyme that is required for reproductive development reveals the potential of developing an enzyme inhibitor. Such an inhibitor would prevent synthesis of the endogenous ligand and thus the requisite activation of DAF-12 for reproductive growth. Indeed, the finding that the broad-spectrum CYP-inhibitor, ketoconazole, was effective at blocking Δ7-DA synthesis in the present study and was shown previously to block developmental activation of cultured L3i (Albarqi et al., 2016) supports this strategy. The strategy for developing CYP inhibitors as drugs is well established (Francis and Delgoda, 2014).
While our study provides a proof of concept for the therapeutic use of an Ss-DAF-12 ligand, we note that Δ7-DA may not be the best candidate for eventual clinical use. Typical of most endogenous steroid receptor ligands, Δ7-DA has a short half-life and relatively poor pharmacokinetic properties in vivo (Patton et al., 2018). For this reason, in our study we administered Δ7-DA continuously in the drinking water over many days. Although overcoming this limitation would be important for developing a suitable therapeutic modality, this is not an unrealistic goal. Encouragingly, in the case of all other nuclear receptors this limitation has been circumvented through the design of potent, long-lasting receptor agonists and antagonists (Chen, 2008; Zhao et al., 2019). Another limitation of this study is that we only tested single doses of Δ7-DA and ivermectin. However, we note that optimizing the dose regimens are likely to reveal even greater cooperative effects. Finally, we have not definitively ruled out the existence of other endogenous DAF-12 ligands. While the findings that Δ7-DA is the only ligand we detected and is present only at the times when DAF-12 would be expected to be an activator are consistent with the conclusion that Δ7-DA is the relevant ligand, it is feasible that other activities exist that were not identified in our purification scheme.
Conclusions
In summary, in this work we identified the endogenous DAF-12 ligand in the human parasitic nematode S. stercoralis, characterized the ligand’s biosynthesis pathway, and demonstrated that activating DAF-12 can overcome the limitation of the front-line drug, ivermectin, and significantly improve the outcome of strongyloidiasis in a preclinical model of hyperinfection. Our study further revealed the ligand’s biosynthetic enzyme as a potential new target that might also be exploited to treat this lethal parasitic disease.
Materials and methods
Animal husbandry
Request a detailed protocolAll animal experiments were approved by the University of Texas Southwestern Medical Center Institutional Animal Care and Use Committee (IACUC) and are listed under animal protocol numbers 2016-101500 and 2018-102369. All protocols, as well as routine husbandry care of the animals, were conducted in strict accordance with the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health (NationalResearchCouncil, 2011). Male dogs of 6–18 months were purchased from Oak Hill Genetics (Ewing, IL) and infected with 3000 L3i of S. stercoralis and were orally administrated 0.25~1.2 mg/kg prednisolone daily to maintain parasite production in feces. Male Mongolian gerbils (TUM/MON strain) of 6–9 weeks were purchased from Charles Rivers (Wilmington, MA) and used as described below.
Reagents
Dafachronic acids were synthesized as reported (Basu et al., 2015; Mahanti et al., 2014; Sharma et al., 2009). Methylprednisolone acetate (MPA) injection suspension (Zoetis, Parsippany, NJ), ketoconazole (Sigma, St. Louis, MO), 8-Br-cGMP (Tocris, Minneapolis, MN), and ivermectin (Merial Limited, Duluth, GA) were purchased as cited. Solvents for lipid extraction and liquid chromatography (toluene, hexanes, isopropanol, methanol, water, and acetonitrile) were purchased from Fisher Scientific (Waltham, MA). Eluent additives for liquid chromatography (LC)–mass spectrometry (MS) (formic acid or NH4Ac) were purchased from Sigma (St. Louis, MO). COS-7 and Sf9 cell lines were purchased from ATCC and are STR certified and mycoplasma-free.
S. stercoralis culture
Request a detailed protocolThe wild-type (UPD strain) of S. stercoralis was maintained in purpose-bred dogs (Oak Hill Genetics, Ewing, IL) and FL stages of S. stercoralis were prepared from coproculture of the dog feces as described (Lok, 2007). Briefly, postparasitic worms at the noted stages were obtained using the Baermann technique (Lok, 2007) from daily collected dog feces (for PP-L1/L2); 24 hr coproculture at 18°C (for FL-L3); 72 hr coproculture at 21°C (for FL-adults and PFL-L1); and 7-day coproculture at 25°C (for L3i). The parasitic stages of S. stercoralis were acquired from Mongolian gerbils (Charles Rivers, Wilmington, MA) as described (Lok, 2007; Stoltzfus et al., 2012). To obtain L3+, gerbils infected with 10,000 L3i larvae were sacrificed 3-day postinfection. After removing the intestinal tract, parenteral tissues were minced and L3+ were collected by the Baermann technique at 37°C. To collect P-adults, intestinal L1–L3, and L3a, gerbils were hyperinfected by an initial inoculation of 4000 L3i and administration of 2 mg MPA, followed by weekly injections of 2 mg MPA for 4 weeks. After sacrificing the gerbils, P-females and intestinal larval stages were collected by hanging the small and large intestines in cylinders filled with saline water supplemented with 0.5 mg/ml gentamicin (Sigma, St. Louis, MO) at 37°C, which allows the parasites to migrate out of the tissues and settle down at bottom of the cylinders. The parasites were then collected and purified by manually removing each stage under a dissection scope. Collected parasites were then washed five times with M9 buffer containing an antibiotic cocktail containing 100 units penicillin, 100 µg/ml streptomycin (Thermo Fisher, Waltham, MA), and 50 μg/ml gentamicin (Sigma, St. Louis, MO) and stored in −80°C for lipid extraction.
Lipid fractionation and ligand identification
Request a detailed protocolEndogenous DAF-12 ligands were purified from the lipid fractions of PP-L3 as outlined (Figure 2—figure supplement 1A). Briefly, ~ 5 million PP-L3s were obtained from ~100 kg dog feces as described above. The worms were pooled and sonicated in 0.9% NaCl, from which crude lipids were extracted by the Folch method (Folch et al., 1957). The crude lipids were dissolved in toluene and 0.1% acetic acid and passed through a Sep-Pak Silica SPE columns (Waters, Milford, MA), followed by sequential elution with hexanes, 30% isopropanol in hexanes, and finally methanol, all of which contains 0.1% acetic acids. The lipids in the 30% isopropanol fraction were then fractionated with semipreparative high-performance liquid chromatography (HPLC) with a C18 column (Luna 5 µm C18 100 Å, 250 × 10 mm, Phenomenex, Torrance, CA) at a flow rate of 3.5 ml/min. The mobile phases were water (A) and acetonitrile (B), both containing 0.1% formic acid. The following gradient was run for a total of 70 min: 0–15 min with 50–100% (B); 15–70 min with 100% (B). Eluted fractions were collected every minute and dried under nitrogen gas and dissolved in ethanol.
HPLC fractions containing endogenous Ss-DAF-12 ligands were first identified using a cell-based reporter assay (Wang et al., 2009). Briefly, COS-7 cells (ATCC, Manassas, VA) cultured in DMEM (Thermo Fisher, Waltham, MA) supplemented with 10% fetal bovine Serum (FBS) (Gemini Bio, West Sacramento, CA) were cotransfected with plasmids expressing the S. stercoralis DAF-12 ligand-binding domain fused in frame with the DNA binding domain from C. elegans DAF-12 (pCMX-CeSs-DAF-12, to increase expression efficiency) (Wang et al., 2009), the lit-1 kinase DAF-12 reporter plasmid (pNL3.1-DAF-12RE-lit1, fused to Nanoluc luciferase) and the control reporter plasmid (pGL4.53, pgk promoter fused to firefly luciferase). The cells were split into 384-well plates and treated with 10 nM Δ7-DA or the HPLC fractions. After 24 hr, Nanoluc and firefly luciferase activities were measured by Nano-Glo Dual-luciferase kits (Promega, Madison, WI) on a Victor V plate reader (Perkin Elmer, Waltham, MA). Relative light units (RLUs) were then calculated by normalizing the Nanoluc luciferase activity to the firefly luciferase activity.
To characterize the chemical identity of the Ss-DAF-12 ligand, isolated HPLC fractions were subjected to ultra-performance liquid chromatography tandem with mass spectrometry (UPLC–MS) using a Shimadzu LC instrument (Shimadzu Scientific Instruments, Inc, Columbia, MD) in tandem with a Sciex 6500 Triple Quad mass spectrometer (AB Sciex LLC, Framingham, MA). The HPLC fractions were diluted in methanol containing 100 nM chenodeoxycholic acid-2H4 (d4-CDCA, Sigma, St. Louis, MO) as an internal control of retention times (RTs) between different runs. The lipids were then loaded onto a C18 column (Kinetex 1.3 µm C18 100 Å, LC Column 50 × 2.1 mm, Phenomenex, Torrance, CA) at a 0.4 ml/min flow rate. The mobile phase consisted of (A) water:acetonitrile (9:1) and (B) acetonitrile:water (99:1), both containing 2 mM NH4Ac. The following gradient was run for a total of 8 min: 60–80% of (B) over 0–2 min; 80% (B) over 2–4 min; 80–99% (B) over 4–6 min; and 99% (B) over 6–8 min. Using negative selective ion monitoring mode, Δ4-DA and Δ7-DA were analyzed at m/z 413 (RT = 1.89 min) and m/z 413 (RT = 2.08 min), respectively; Δ1,7-DA was analyzed at m/z 411 (RT = 1.95 min). The identity of the peaks was determined by comparing to standards that were run in parallel with the lipid fractions.
Quantitative analysis of Δ7-DA levels in S. stercoralis
Request a detailed protocolS. stercoralis at different developmental stages were prepared as described above and sample preparation is summarized in Table 2. For the environmental postparasitic stages (PP-L1, FL-L3, FL-adults, PFL-L1, and L3i), worms were lysed by sonication in 0.9% NaCl. For the host-derived parasitic stages (L3+, P-adult, and intestinal L1-L3a) and cGMP-treated L3i, worms were lysed in digestion buffer (2 mM Tris–HCl, pH 8.0, 1 mg/ml Proteinase K, and 0.25% SDS) at 55°C for 2 hr. SDS was then precipitated and removed by adding a saturating amount of KCl followed by centrifugation at 10,000 × g for 5 min. For quantification, 100 nM Δ7-DA was added as a standard to lipid fractions from stages that do not have endogenous Δ7-DA (listed in Table 1) and were processed in parallel with other samples. Lysates from parasite samples and standards were extracted by the Folch method and lipids were dried under nitrogen. Δ7-DA in the lipids was then derived to Δ7-DA-picolylamine (Δ7-DA-PA) for analysis by incubating the lipids with 3.3 mM of triphenylphosphine, 3.3 mM 2,2′-dipyridyl disulfide, and 333 ng/µl 2-picolylamine at 60°C for 20 min (Li et al., 2013). The Δ7-DA-PA was then analyzed by UPLC–MS. The UPLC method was same as described above and Δ7-DA-PA compounds were detected in positive multiple reaction monitoring (MRM) mode with m/z transition 505 → 487. DA quantification was then performed with the software MultiQuant (AB Sciex LLC, Framingham, MA) by comparing Δ7-DA-PA peak areas in the parasite samples with those of 100 nM Δ7-DA standards.
Biosynthesis of Δ7-DA from dietary cholesterol in S. stercoralis
Request a detailed protocolPP-L1 stages of S. stercoralis were prepared from dog feces as described above and cultured on NGM plates modified for metabolic tracing of isotope-labeled cholesterol supplemented in the diet. Briefly, HB101 bacteria were cultured overnight in cholesterol-free medium (10 g/l ether-extracted peptone in DMEM) and prepared as 10× concentrates supplemented with 140 μM naturally labeled (i.e., 12C) or 13C3 (Cambridge Isotope Laboratories, Tewksbury, MA) labeled cholesterol. The bacteria concentrates were then spotted on cholesterol-free NGM plates (3% agarose in S-basal) to form bacterial lawns. Approximately 10,000 PP-L1 larvae were cultured on the plates at 18°C for 24 hr and collected and analyzed as described above for Δ7-DA-PA-13C3 levels by UPLC–MS in the positive MRM mode with m/z transition 508 →490.
In vitro enzyme activity assays
Request a detailed protocolCandidate enzyme genes (Stoltzfus et al., 2012) were cloned and each gene expressed in Sf9 cells (ATCC, Manassas, VA), which are cultured with Sf 900 III medium (Thermo Fisher, Waltham, MA). A human CYP450 oxidoreductase (hOR) was coexpressed to facilitate electron transport required for the enzyme activities (Motola et al., 2006). Enzyme coding sequences were tethered with a C-terminal HA tag in pFastBac Dual baculoviral vectors (Invitrogen, Waltham, MA) using Ph or p10 expression cassettes for hOR or other enzymes, respectively. Baculovirus was prepared according to manufacturer’s instructions followed by infection of Sf9 cells. For DAF-36 and DHS-16 homologs, the microsomes expressing the enzymes were purified from the infected Sf9 cells with a Microsome Isolation Kit (Abcam, Cambridge, MA). Microsomes were incubated with an NADPH regeneration system (Promega, Madison, WI) in presence or absence of 100 μM substrate compounds at 37°C for 16 hr. For CYP activities, infected Sf9 cells were incubated with 10 μM substrate compound at 28°C for 24 hr. The substrate compounds for DAF-36, DHS-16 and CYPs were cholesterol-2H7, lathosterol-2H7 (Avanti Polar Lipids, Inc, Alabaster, AL), and lathosterone (Steraloids, Inc, Newport, RI), respectively. Lipids were then extracted from microsomes (for DAF-36s and DHS-16s) or Sf9 cells (for CYPs) by the Folch method and loaded onto a C18 column (Kinetex 1.3 µm C18 100 Å, LC Column 50 × 2.1 mm, Phenomenex, Torrance, CA) at a 0.4 ml/min flow rate. The mobile phase consisted of (A) water:acetonitrile (9:1) and (B) acetonitrile:water (99:1), both containing 2 mM NH4Ac. For measuring the activities of the DAF-36 and DHS-16 homologs, the following gradient was run for a total of 8 min: 80–99% (B) for 0–2 min; 99% (B) for 2–8 min. Enzyme products were detected in positive MRM mode with m/z transition 374 → 109 (7-dehydrocholesterol-2H7) and 392 → 109 (lathosterone-2H7). For CYP activities, Δ7-DA was analyzed by the UPLC–MS methods described above.
L3i activation assay
Request a detailed protocolReactivation of L3i developmental arrest was assayed by monitoring feeding activity as described (Albarqi et al., 2016; Cheong et al., 2021). Briefly, L3i larvae prepared as described above were suspended in M9 buffer at 100 larvae/96 well. The larvae were treated with 0.5 mM 8-Br-cGMP (Torcis, Minneapolis, MN), 1 μM Δ7-DA and/or 25 μM of ketoconazole (Sigma, St. Louis, MO) at 37°C for 22 hr. To visualize the feeding behaviors, worms were incubated with 200 μg/ml of Alexa 594 fluorescent dye (Thermofisher, Waltham, MA) at 37°C for another 3 hr, washed and observed under fluorescent microscope. Worms with internal red fluorescence were scored as feeding larvae. For Δ7-DA levels, worms were suspended in M9 buffer at 4000 larvae/ml and treated for the indicated times. Δ7-DA levels were then analyzed by UPLC–MS as described above.
QPCR
Request a detailed protocolQPCR was performed by SYBR green method as reported (Wang et al., 2015). Briefly, 10,000–20,000 L3i larvae were pelleted and RNA extracted with RNA-STAT60 reagent (Amsbio, Cambridge, MA). Total RNA was digested with Turbo-DNAase (Ambion Inc, Austin, TX) and purified with RNeasy Mini Kits (Qiagen, Germantown, MD) to remove genomic DNA. Following reverse transcription, Ss-cyp22a9 and Ss-18S rRNA levels were analyzed by QPCR (primer sequences in Table 3) and presented as relative mRNA levels (Ss-cyp22a9/Ss-18S rRNA).
Immunoblotting
Request a detailed protocolWestern immunoblotting was performed following standard protocol. Sf9 cells expressing Ss-CYP450 enzymes were pelleted and lysed in Laemmli sample buffer by sonication followed by 5 min in a boiling water bath and 2 min on ice. The samples were then analyzed by 10% SDS–PAGE transferred to nitrocellulose membrane, and immunoblotted with the mouse anti-HA antibody [HA.C5] (Abcam, Cambridge, MA) followed by HRP-conjugated anti-mouse antibody (Abcam, Cambridge, MA). The membranes were then imaged by ImageQuant LAS4000 luminescent Image Analyzer (GE Healthcare, Chicago, IL) following an incubation with ECL chemiluminescent substrate reagent kit (Thermo Fisher, Waltham, MA).
CRISPR gene disruption
Request a detailed protocolTo knock out expression of the gene encoding the DAF-9 ortholog, Ss-cyp22a9, CRISPR methods developed specifically for S. stercoralis were employed as outlined (Figure 5—figure supplement 1) and previously reported (Cheong et al., 2021; Gang et al., 2017; Lok, 2019). Briefly, an sgRNA targeting Ss-cyp22a9 was designed by GPP sgRNA Designer (Broad Institute) and CHOPCHOP (http://chopchop.cbu.uib.no/). Top candidates with 5′-(N)18GG-3′ formats from both designs were compared to the S. stercoralis genome (https://parasite.wormbase.org/Strongyloides_stercoralis_prjeb528/Info/Index/) for off-targeting analysis. The sequence 5′-GCTGGGGACTTATGGACAGG-3′ was selected as only targeting the Ss-cyp22a9 locus within the entire S. stercoralis genome and cloned into the gRNA vector pML60 (Gang et al., 2017). Ss-unc-22 sgRNA (gift from Dr. Ellisa A. Hallem, UCLA) was used as a control. CRISPR gene editing was accomplished using both homologous directed repair (HDR) and nonhomologous end joining (NHEJ) and vectors as previously published (Gang et al., 2017; Lok, 2019). For HDR, a homology repair template was made by sewing ~500 bp homology arms flanking the Cas9 cutting site at the Ss-cyp22a9 locus with a GFP expressing cassette (Sr-eef-1p::GFP::era, from pPV529) (Figure 5—figure supplement 1C) by fusion PCR. At least 50 FL females of S. stercoralis were microinjected with plasmids expressing sgRNA (60 ng/µl), Cas9 (pPV540, 20 ng/µl), and the homology repair template (10 ng/µl). Injected females were then placed with adult males in the fecal culture as described (Cheong et al., 2021; Gang et al., 2017; Lok, 2019). The F1 progenies from these matings were collected after 7-day fecal culture at 25°C with the Baermann technique. The GFP-positive L3i larvae from the F1 progenies were manually picked and treated with 0.5 mM 8-Br-cGMP or 1 μM Δ7-DA in M9 buffer at 37°C for 22 hr. The feeding behaviors were then visualized as described above and genotyped individually by single worm PCR with the primers listed in Table 3.
In a second set of experiments, NHEJ CRISPR was used to produce enough Ss-cyp22a9 knockout worms for detection of endogenous Δ7-DA. As described above, 50 FL females of S. stercoralis were microinjected with plasmids expressing Cas9 (pPV540, 20 ng/µl) and sgRNAs against Ss-cyp22a9 or Ss-unc-22 (60 ng/µl). Following a fecal culture with FL males at 25°C for 7 days, the resulting L3i larvae were collected by the Baermann technique and treated by 0.5 mM 8-Br-cGMP in M9 buffer for 3 days. Levels of Δ7-DA were analyzed by UPLC–MS as described above.
Gerbil models of S. stercoralis infection
Request a detailed protocolUncomplicated and hyperinfection cases of strongyloidiasis were established in Mongolian gerbils (Charles Rivers, Wilmington, MA) as described (Nolan et al., 1993). For uncomplicated strongyloidiasis, gerbils were subcutaneously injected with 1000 L3i S. stercoralis larvae. On day 21 post the infection, the gerbils were switched to drinking water containing 5% sucrose (vehicle) or 50 μM Δ7-DA in 5% sucrose. We have found that adding a small amount of sucrose does not affect infection, but ensures animals drink appropriate amounts of drug. Fecal larval numbers were monitored biweekly for the next 14 days. To establish hyperinfection, gerbils were infected similarly but MPA (2 mg/gerbil) was injected subcutaneously weekly from the time of infection. After 10-day postinfection to allow time for the hyperinfection to occur, Δ7-DA treatment was begun as noted above. Ivermectin (Merial Limited, Duluth, GA) was intraperitoneally injected at 300 µg/kg on day 21 postinfection. Since Δ7-DA is rapidly turned over in vivo (Patton et al., 2018), administering it at an earlier time point ensured an adequate amount was delivered. Animals were observed daily for irreversible hyperinfection symptoms that indicate imminent lethality, which include lethargy, hunched back, scruffy hair, lack of activity, and reduced drinking. Animals with such symptoms were sacrificed and autopsy performed to determine end-point parasite numbers as described below.
Parasite burden analysis
Request a detailed protocolParasite numbers in gerbils were determined as described with modifications (Lok, 2007; Nolan et al., 1993; Stoltzfus et al., 2012). For fecal larvae outputs, infected gerbils were housed in wire bottom cages with a layer of dampened paper towel for 8 hr. Feces (15–20 droppings) were collected and macerated in 0.9% NaCl. The fecal suspension was then filtered through a layer of cheese cloth and an aliquot of the suspension was spotted on NGM plates. After drying, plates were incubated at 37°C for 10 min and living larvae, which leave traces on the plates, were counted and normalized to the feces weight as larvae per gram feces. To determine end-point parasite burden, intestines were cut longitudinally and incubated in 0.9% NaCl supplemented with an antibiotic cocktail (100 units/ml penicillin, 100 μg/ml streptomycin and 50 μg/ml gentamicin) at 37°C for 2–3 hr. The suspension was then filtered through a layer of cheese cloth and an aliquot of the suspension was spotted on NGM plates. Following drying and 10 min incubation at 37°C, parasitic adults and intestinal L1–L3a larvae were counted. To determine parasite burden of hyperinfected L3+, parenteral tissues were dissected, minced and larvae collected by the Baermann method were counted on watch glasses.
Data analysis
Request a detailed protocolData were plotted and analyzed by the indicated statistical tests using GraphPad Prism 8 software to obtain p values. When multiple comparisons were involved, pairwise statistical analyses were first performed to obtain p values and then adjusted by the false discovery rate method using the p.adjust function in R to obtain q values (https://www.R-project.org/).
Data availability
All data are presented in the manuscript; source data files have been provided for Figure 4—figure supplement 1 and Figure 5B.
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WormBase ParaSiteID GCA_000947215.1. Strongyloides stercoralis.
References
-
Strongyloides stercoralis: a plea for actionPLOS Neglected Tropical Diseases 7:e2214.https://doi.org/10.1371/journal.pntd.0002214
-
Severe strongyloidiasis: a systematic review of case reportsBMC Infectious Diseases 13:78.https://doi.org/10.1186/1471-2334-13-78
-
Nuclear receptor drug discoveryCurrent Opinion in Chemical Biology 12:418–426.https://doi.org/10.1016/j.cbpa.2008.07.001
-
A simple method for the isolation and purification of total lipides from animal tissuesThe Journal of Biological Chemistry 226:497–509.https://doi.org/10.1016/S0021-9258(18)64849-5
-
A patent review on the development of human cytochrome P450 inhibitorsExpert Opinion on Therapeutic Patents 24:699–717.https://doi.org/10.1517/13543776.2014.899583
-
Targeted mutagenesis in a human-parasitic nematodePLOS Pathogens 13:e1006675.https://doi.org/10.1371/journal.ppat.1006675
-
DauerWormBook: The Online Review of C. elegans Biology 1:1–19.https://doi.org/10.1895/wormbook.1.144.1
-
DAF-9, a cytochrome P450 regulating C. elegans larval development and adult longevityDevelopment 129:221–231.
-
A public health response against Strongyloides stercoralis: time to look at soil-transmitted helminthiasis in fullPLOS Neglected Tropical Diseases 7:e2165.https://doi.org/10.1371/journal.pntd.0002165
-
Absolute quantification of a steroid hormone that regulates development in Caenorhabditis elegansAnalytical Chemistry 85:9281–9287.https://doi.org/10.1021/ac402025c
-
Strongyloides stercoralis: a model for translational research on parasitic nematode biologyWormBook: The Online Review of C. elegans Biology 1:1–18.https://doi.org/10.1895/wormbook.1.134.1
-
Clinical features of Strongyloides stercoralis infection in an endemic area of the United StatesGastroenterology 80:1481–1488.
-
BookGuide for the Care and Use of Laboratory AnimalsThe National Academies Press.https://doi.org/10.17226/12910
-
Strongyloides stercoralis: the first rodent model for uncomplicated and hyperinfective strongyloidiasis, the Mongolian gerbil (Meriones unguiculatus)The Journal of Infectious Diseases 168:1479–1484.https://doi.org/10.1093/infdis/168.6.1479
-
The Unique Life Cycle of Strongyloides stercoralis and Implications for Public Health ActionTropical Medicine and Infectious Disease 3:E53.https://doi.org/10.3390/tropicalmed3020053
-
Ivermectin resistance and overview of the Consortium for Anthelmintic Resistance SNPsExpert Opinion on Drug Discovery 2:S41–S52.https://doi.org/10.1517/17460441.2.S1.S41
-
Strongyloidiasis Outside Endemic Areas: Long-term Parasitological and Clinical Follow-up After Ivermectin TreatmentClinical Infectious Diseases 66:1558–1565.https://doi.org/10.1093/cid/cix1069
-
The biology of Strongyloides sppWormBook: The Online Review of C. elegans Biology 1:1–17.https://doi.org/10.1895/wormbook.1.141.2
-
Nuclear receptors: emerging drug targets for parasitic diseasesThe Journal of Clinical Investigation 127:1165–1171.https://doi.org/10.1172/JCI88890
-
Nuclear Receptors: Recent Drug Discovery for Cancer TherapiesEndocrine Reviews 40:1207–1249.https://doi.org/10.1210/er.2018-00222
Article and author information
Author details
Funding
National Institutes of Health (AI105856)
- James B Lok
- Steven A Kliewer
- David J Mangelsdorf
National Institutes of Health (GM141088)
- Tian Qin
National Institutes of Health (AI050886)
- James B Lok
Welch Foundation (I-1275)
- David J Mangelsdorf
Welch Foundation (I-1558)
- Steven A Kliewer
Welch Foundation (I-2010-20190330)
- Tian Qin
Howard Hughes Medical Institute
- David J Mangelsdorf
The funders had no role in study design, data collection, and interpretation, or the decision to submit the work for publication.
Acknowledgements
We thank members of the Mango/Kliewer lab and Dr. Jeffrey McDonald (UT Southwestern) for fruitful discussions; Dr. Elissa Hallem (UCLA) for providing CRISPR plasmids; Adeiye Pilgrim (Emory) for assistance with cloning. This work was supported by the National Institutes of Health (grant AI105856 to JBL, DJM, and SAK, GM141088 to TQ, and AI050886 to JBL), the Robert A Welch Foundation (grants I-1275 to DJM, I-1558 to SAK, and I-2010-20190330 to TQ), UT Southwestern Eugene McDermott Scholarship (TQ), and the Howard Hughes Medical Institute (DJM).
Ethics
All animal experiments were approved by the University of Texas Southwestern Medical Center Institutional Animal Care and Use Committee (IACUC) and are listed under animal protocol numbers 2016-101500 and 2018-102369. All protocols, as well as routine husbandry care of the animals, were conducted in strict accordance with the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health (National Research Council, 2011).
Copyright
© 2021, Wang et al.
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
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- Microbiology and Infectious Disease
Patients present a wide range of clinical severities in response severe acute respiratory syndrome coronavirus 2 infection, but the underlying molecular and cellular reasons why clinical outcomes vary so greatly within the population remains unknown. Here, we report that negative clinical outcomes in severely ill patients were associated with divergent RNA transcriptome profiles in peripheral immune cells compared with mild cases during the first weeks after disease onset. Protein–protein interaction analysis indicated that early-responding cytotoxic natural killer cells were associated with an effective clearance of the virus and a less severe outcome. This innate immune response was associated with the activation of select cytokine–cytokine receptor pathways and robust Th1/Th2 cell differentiation profiles. In contrast, severely ill patients exhibited a dysregulation between innate and adaptive responses affiliated with divergent Th1/Th2 profiles and negative outcomes. This knowledge forms the basis of clinical triage that may be used to preemptively detect high-risk patients before life-threatening outcomes ensue.