Glial Nrf2 signaling mediates the neuroprotection exerted by Gastrodia elata Blume in Lrrk2-G2019S Parkinson’s disease
Abstract
The most frequent missense mutations in familial Parkinson’s disease (PD) occur in the highly conserved LRRK2/PARK8 gene with G2019S mutation. We previously established a fly model of PD carrying the LRRK2-G2019S mutation that exhibited the parkinsonism-like phenotypes. An herbal medicine, Gastrodia elata Blume (GE), has been reported to have neuroprotective effects in toxin-induced PD models. However, the underpinning molecular mechanisms of GE beneficiary to G2019S-induced PD remain unclear. Here, we show that these G2019S flies treated with water extracts of GE (WGE) and its bioactive compounds, gastrodin and 4-HBA, displayed locomotion improvement and dopaminergic neuron protection. WGE suppressed the accumulation and hyperactivation of G2019S proteins in dopaminergic neurons and activated the antioxidation and detoxification factor Nrf2 mostly in the astrocyte-like and ensheathing glia. Glial activation of Nrf2 antagonizes G2019S-induced Mad/Smad signaling. Moreover, we treated LRRK2-G2019S transgenic mice with WGE and found that the locomotion declines, the loss of dopaminergic neurons, and the number of hyperactive microglia were restored. WGE also suppressed the hyperactivation of G2019S proteins and regulated the Smad2/3 pathways in the mice brains. We conclude that WGE prevents locomotion defects and the neuronal loss induced by G2019S mutation via glial Nrf2/Mad signaling, unveiling a potential therapeutic avenue for PD.
Editor's evaluation
Your study elucidates the molecular mechanisms by which a Chinese traditional medicine compound, GE, confers neuroprotection in mouse and fly models of LRRK2 G2019S-induced Parkinson's disease and provides an avenue for a potential therapeutic treatment for patients.
https://doi.org/10.7554/eLife.73753.sa0eLife digest
Parkinson’s disease is a brain disorder that leads to tremors and difficulties with balance and coordination. These symptoms are caused by the loss of neurons which release a chemical messenger that is needed to regulate movement called dopamine. Most treatments for this disease work by boosting levels of dopamine in the brain, but this can lead to severe side effects and these drugs often become less effective over time.
A traditional Chinese medicine called Gastrodia elata Blume (or GE for short) has previously been reported to relieve symptoms of Parkinson’s disease in both human and animal studies when administered as a decoction or formula. However, it is unclear how GE protects dopamine-producing neurons and if this mechanism involves another type of brain cell known as glia that has also been linked to Parkinson’s disease.
To investigate, Lin et al. studied fruit flies and mice that carry a genetic mutation that produces the symptoms and molecular characteristics of Parkinson’s disease. The experiments showed that when the flies and mice were fed food containing water extracts of GE, they experienced less difficulties moving and had a higher number of intact dopamine-producing neurons.
Lin et al. found that GE switched on a protein in glial cells located near dopamine-producing neurons. Activation of this protein, called Nrf2, inhibited a signaling pathway in degenerating neurons that leads to the disease state. As a result, less dopamine-producing neurons were damaged and the animals’ coordination and balance were maintained.
These findings suggest that GE could potentially provide an alternative or complementary therapy for Parkinson’s disease, although it still needs to be studied further in humans. If the same effect is observed, the specific compounds in GE that have this protective effect could be isolated and analyzed to see if they could be used for treatment.
Introduction
Parkinson’s disease (PD) is a highly prevalent neurodegenerative disorder characterized by the loss of dopaminergic neurons in the substantia nigra projecting to the striatum of the basal ganglion, representing a circuit involved in motor planning and coordination. As a consequence, PD is associated with motor abnormalities, bradykinesia, hypokinesia, rigidity, and resting tremor. Currently, the most frequently applied pharmacological treatment, levodopa (L-DOPA), exerts limited motor improvement and elicits negative side effects (Ray Chaudhuri et al., 2018). Hence, identifying and developing alternative or complementary treatments may assist in mitigating PD progression.
PD is a multicausal disease with a complicated etiology, including familial inheritance. More than 20 PARK genes have been genetically linked to PD, a number that is increasing (Houlden and Singleton, 2012). Missense mutations in PARK8, or Leucine-rich repeat kinase 2 (LRRK2), induce characteristic PD symptoms and pathologies such as loss of dopaminergic neurons and the appearance of Lewy bodies (Martin et al., 2014). Notably, the most commonly observed mutation, dominant G2019S, among familial PD cases is located in the kinase domain of Lrrk2, which augments its kinase activity via auto- and hyperactivation (Sheng et al., 2012). The hyperactive G2019S mutant protein alters several cellular processes, including vesicle trafficking, microtubule dynamics, autophagy, mitochondrial function (Martin et al., 2014), and, most commonly, increases susceptibility to oxidative stress that contributes to neuronal degeneration (Angeles et al., 2011; Nguyen et al., 2011). These indicate that regulation of the hyperactivation of G2019S mutant protein appears to be a disease-modifying strategy.
Glia provide structural and metabolic supports to neurons and regulate synaptic transmissions, so they are important for the function and survival of dopaminergic neurons (Lin et al., 1993; Sofroniew and Vinters, 2010). Dysfunction in two major glial types, astrocytes and microglia, contribute to the onset and progression of both sporadic and familial PD (Kam et al., 2020). Astrocytes and microglia of postmortem PD brains exhibit pathological lesions and hyper-immunoactivity (Miklossy et al., 2006). A clinical trial involving downregulation of microglial oxidative stress highlights the significance of glia to PD (Jucaite et al., 2015). Moreover, Lrrk2 regulates the inflammatory response in microglia and the autophagy-lysosome pathway in astrocytes, with the G2019S mutation altering the size and pH of lysosomes (Henry et al., 2015; Moehle et al., 2012). Expression of G2019S mutant protein in neurons was previously shown to induce the secretion of Glass bottom boat (Gbb)/bone morphogenetic protein (BMP) signal that, in turn, upregulates Mothers against decapentaplegic (Mad)/Smad signaling in glia, which prompts feedback signals to promote neuronal degeneration (Maksoud et al., 2019). These studies indicate that G2019S mutant protein alters the homeostasis and interaction between neurons and glia, contributing to PD pathogenesis. However, whether any dietary or pharmacological treatment blockading this neuron-glia interaction beneficiary to G2019S-induced PD is unclear.
Given that up to 70% of human PARK genes are conserved in the Drosophila genome, Drosophila is frequently used as a PD model for studying gene function, such as the PARK1/SNCA (Chen and Feany, 2005) and PARK8/LRRK2 (Liu et al., 2008). Genetic and molecular linkage between PINK1/PARK6 and Parkin/PARK2 was first established in Drosophila (Clark et al., 2006). When overexpressed in Drosophila dopaminergic neurons, LRRK2 transgenes carrying G2019S or other dominant mutations induce dopaminergic neuron loss and locomotion impairment, two age-dependent symptoms of PD (Lin et al., 2010; Liu et al., 2008). The G2019S model has further been used to screen a collection of FDA-approved drugs to suppress these PD phenotypes. Thus, Drosophila represents an amenable model of PD for genetic, molecular, and pharmacological study of potential therapeutic interventions. Strikingly, lovastatin was found to prevent dendrite degeneration, dopaminergic neuron loss, and impaired locomotion, and, critically, a lovastatin-involved Nrf2 pathway proved neuroprotective (Lin et al., 2016a). Nevertheless, whether the Nrf2-mediated neuroprotection is cell- or non-cell-autonomous remains elusive.
Traditional Chinese Medicine (TCM) is often used as an alternative or dietary treatment for human diseases, including PD (Kim et al., 2012; Li et al., 2017). Although the results were inconclusive, some TCM could display adjuvant effects when used in combination with L-DOPA, reducing the L-DOPA dosage required in long-term treatments and relieving non-motor symptoms (Kim et al., 2012). As a prominent component in TCM, Gastrodia elata Blume (GE; Orchidaceae) has been used to treat neurological disorders for centuries (Chen and Sheen, 2011). GE has been shown to exert neuroprotective, anti-inflammatory, and antioxidative effects in neurodegenerative disease models (Jang et al., 2015). The major bioactive compounds in GE include gastrodin and 4-hydroxybenzyl alcohol (4-HBA), both of which display pharmacological effects on neurobiological and psychological disorders (Chen et al., 2016; Kumar et al., 2013). Additionally, gastrodin and 4-HBA have been reported to activate the Nrf2 signaling in dopaminergic neurons and astrocytes, respectively (Jiang et al., 2014; Luo et al., 2017), highlighting a potential benefit of incorporating GE in PD treatments. However, the effects and mechanisms underlying how GE moderate Lrrk2-G2019S PD remain unclear.
In the present study, we treated G2019S animals with water extract of GE (WGE), and its bioactive compounds, gastrodin and 4-HBA. We have investigated the impact of WGE treatment on PD in restoring locomotion and protecting dopaminergic neurons in the Drosophila G2019S model. We identified two distinct pathways induced by WGE in the model, that is, suppression of Lrrk2 protein accumulation and hyperphosphorylation in neurons, and activation of the Nrf2 pathway in glia, particularly in astrocyte-like and ensheathing glia. We show that WGE-induced Nrf2 activation antagonizes the Gbb-activated Mad signaling in glia, contributing to neuronal protection. WGE also suppressed the hyperactivation of G2019S proteins and antagonized Smad2/3 signaling in a LRRK2-G2019S mouse model, which restored locomotion, protected dopaminergic neurons, and regulated the microglia hyperactivation. Conservation of the pathways impacted by WGE treatment in both the Drosophila and mouse G2019S models implies the beneficial effects of GE and represent a reliable and effective complementary therapy for PD.
Results
WGE treatment improves locomotion of Ddc>G2019S flies
We employed the GAL4-UAS system to express the human G2019S mutant of Lrrk2 by the Ddc-GAL4 driver (Ddc>G2019S) in dopaminergic neurons and then assessed the anti-geotactic climbing activity of adult flies. We observed that locomotion of Ddc>G2019S flies was affected significantly relative to control flies expressing human wild-type Lrrk2 (Ddc>Lrrk2) (Figure 1A, Figure 1—figure supplement 1A). At weeks 1 and 2, more than 80% of Ddc>G2019S flies could successfully climb above an 8 cm threshold, a proportion comparable to that of Ddc>Lrrk2 flies. However, the success rate declined to ~40% at week 3, ~ 20% at week 4, and to less than 10% at weeks 5 and 6. These proportions are significantly lower than the ~80% at week 3, ~60% at week 4, and ~40% at weeks 5 and 6 displayed by Ddc>Lrrk2 flies. Although both Ddc>G2019S and Ddc>Lrrk2 flies failed to reach the 8 cm mark at weeks 7 and 8, Ddc>Lrrk2 flies could still climb the wall, whereas almost all Ddc>G2019S flies could not (Figure 1—figure supplement 1A). We also tested the climbing activity of another control expressing GFP in dopaminergic neurons (Ddc>mCD8-GFP). Both Ddc>Lrrk2 and Ddc>mCD8-GFP flies showed comparable climbing activities in the first six weeks, and a significant number of Ddc>mCD8-GFP flies were still able to climb above the 8 cm mark at week 7. Therefore, we used the Ddc>Lrrk2 line as a control for Ddc>G2019S flies in subsequent experiments to dissect the specific mode of pathogenicity of the G2019S mutation.
Next, we examined the effect of feeding flies with WGE as a dietary supplement at different concentrations (0.1, 0.5, or 1.0%, w/w). WGE treatment of Ddc>G2019S flies at all three doses elicited a significant improvement in their climbing ability (Figure 1A). Strikingly, the lowest concentration (0.1%) of WGE proved the most effective, with Ddc>G2019S flies still performing well at climbing (i.e., comparably to Ddc>Lrrk2 control flies) in weeks 5 and 6. The higher doses of WGE (0.5 and 1.0%) still exerted beneficial effects at weeks 3 and 4, albeit not as significantly as the 0.1% dose, but had no beneficial effect in weeks 5 and 6. As 0.1% is the lowest among the three doses tested, we lowered the dose of WGE to 0.02% and found that 0.02% WGE was less effective than 0.1% starting at week 3 till week 6, suggesting that 0.1% is the optimal dose in restoring the climbing activity of Ddc>G2019S flies (Figure 1—figure supplement 1B). We also tested the WGE effect on the Ddc>G2019S flies that were fed with regular food without WGE for 3 weeks. At week 4, these Ddc>G2019S flies also showed a significant improvement in their climbing ability compared to the age-matched Ddc>G2019S flies fed continuously on regular food (Figure 1—figure supplement 2). The effect of improving climbing activity in the WGE-fed Ddc>G2019S flies was reduced at week 5 and diminished at week 6, suggesting that WGE feeding starting at earlier stages is important for long-term locomotion improvement.
Gastrodin and 4-HBA are two major phenolic compounds in GE displaying neuropharmacological effects (Zhan et al., 2016). Feeding Ddc>G2019S flies with food containing gastrodin (0.1 mM) equivalent to the amount in 0.1% WGE also restored locomotion of Ddc>G2019S flies in weeks 2–4, though its impact diminished to nonsignificant levels at weeks 5 and 6. However, increasing the gastrodin dose 10-fold (1.0 mM) resulted in improved climbing activity at weeks 5 and 6 (Figure 1B). Similarly, the equivalent 0.1 mM of 4-HBA, the aglyconic form of gastrodin and the bioactive form in the brain (Wu et al., 2017), was sufficient to restore the climbing ability of Ddc>G2019S flies, and a 10-fold dose at 1.0 mM had an even better effect (Figure 1C). These results indicate that both gastrodin and 4-HBA are primary bioactive compounds in GE that prevent locomotion decline in Ddc>G2019S flies, and higher doses are more beneficial to reach the effect as WGE did.
Success in the antigravity wall-climbing assay also requires an immediate response to startle knockdown. Accordingly, we performed a second assay, free-walking in an open arena, to assess improved locomotion. Consistently, free-walking by Ddc>G2019S flies was greatly impaired, with total walking distance reduced to less than 20% that displayed by control Ddc>Lrrk2 flies (Figure 1D). Moreover, Ddc>G2019S flies displayed centrophobism, that is, they avoided walking into the central open space. Strikingly, 0.1% WGE feeding greatly extended walking distance and suppressed the centrophobism of Ddc>G2019S flies. Together, these two assays strongly indicate that the defective locomotion exhibited by Ddc>G2019S flies is greatly improved by feeding them with 0.1% WGE. Because the improvement on the locomotion was more effective in flies fed with 0.1% WGE than the pure compounds, we therefore fed the flies with 0.1% WGE in the following experiments.
WGE treatment suppresses dopaminergic neuron loss in Ddc>G2019S brain
Expression of G2019S mutant protein has been shown to induce a gradual loss of dopaminergic neurons in the adult fly brain, contributing to impaired locomotion (Lin et al., 2010; Liu et al., 2008). Several clusters of dopaminergic neurons have been identified in the adult brain of Drosophila. Here, we focused on the PPL1, PPL2, PPM1/2, and PPM3 clusters that have well-defined roles in modulating locomotion (Mao and Davis, 2009) to assess the effect of WGE treatment. We detected reduced numbers of dopaminergic neurons in the PPL1, PPL2, PPM1/2, and PPM3 clusters of Ddc>G2019S flies relative to Ddc>Lrrk2 controls, which increased in severity from weeks 2 to 6 (Figure 2, Figure 2—figure supplement 1A–C). Consistently, feeding Ddc>G2019S flies with 0.1% WGE restored numbers of dopaminergic neurons in these clusters to the levels observed in controls (Figure 2, Figure 2—figure supplement 1A–C). Thus, concomitant rescue of locomotion and dopaminergic neuron populations in Ddc>G2019S flies indicates that WGE treatment likely promotes dopaminergic neuron survival to restore locomotion.
WGE treatment suppresses G2019S-induced Lrrk2 hyperactivation
The enhanced survival of dopaminergic neurons due to WGE treatment implies that WGE induces neuroprotective mechanisms in Ddc>G2019S flies. The G2019S mutation causes Lrrk2 hyperphosphorylation, protein accumulation, and aberrant cellular signaling (Price et al., 2018). Therefore, we explored if WGE-induced neuroprotection is responsible for abrogating these processes in G2019S flies. We detected comparable levels of Lrrk2 proteins in 3-day-old adult brains pan-neuronally expressing wild-type Lrrk2 (elav>Lrrk2) or G2019S mutant protein (elav>G2019S) (Figure 3A). However, phosphorylation levels at the Ser1292 autophosphorylation site were higher in the elav>G2019S flies compared to elav>Lrrk2 (Figure 3A and B). This outcome was also observed at week 4 (Figure 3F and G), consistent with the idea that Lrrk2 is hyperactivated upon G2019S mutation. Hyperphosphorylation in elav>G2019S flies led to Lrrk2 protein accumulation, as determined by total Lrrk2 levels at weeks 2 and 4 (Figure 3D and E). However, both hyperphosphorylation and protein accumulation were suppressed upon feeding elav>G2019S flies with 0.1% WGE (Figure 3D–G). In the elav>Lrrk2 control, WGE feeding had no effect on levels of wild-type Lrrk2 or Ser1292 phosphorylation (Figure 3—figure supplement 1A–C). We further examined the G2019S mutant-activated downstream effector Rab10, the phosphorylation status of which can serve as an indicator of Lrrk2 kinase activity (Karayel et al., 2020). We observed that levels of phosphorylated Rab10 were increased in elav>G2019S flies, but this phenotype was suppressed by WGE feeding (Figure 3F and H). Hence, feeding flies with WGE suppresses G2019S-induced Lrrk2 protein phosphorylation, accumulation, and signaling.
WGE treatment restores Akt/GSK3β/Nrf2 pathway activity
The Akt/GSK3β/Nrf2 signaling axis has been shown to promote survival of dopaminergic neurons and ameliorate motor dysfunction in PD models (Lin et al., 2016b). We assayed the phosphorylation status of Akt at Ser505 in 3-day-old adult fly head extracts and found reduced levels of pAkt in elav>G2019S flies relative to elav>Lrrk2 controls (Figure 3A and C). This pAkt reduction persisted at weeks 2 and 4, but was abrogated by feeding elav>G2019S flies with 0.1% WGE (Figure 4A and B). We also examined phosphorylation levels of Nrf2 at Ser40 and GSK3β at Ser9, both of which were reduced upon expression of G2019S mutant protein and were equally offset by WGE feeding (Figure 4C and D). Induction of Akt/GSK3β/Nrf2 signaling activates expression of heme oxygenase 1 (HO-1), and we observed diminished levels of this latter protein in elav>G2019S flies, which could be rescued by WGE feeding (Figure 4C and D). Therefore, WGE feeding restores the Akt/GSK3β/Nrf2 signaling activity compromised by the G2019S mutation.
Glial Nrf2 mediates the beneficial effect of WGE on restored locomotion and neuronal protection in G2019S flies
Restoration of Akt/GSK3β/Nrf2 signaling activity by WGE treatment prompted us to test by genetic assays if that signaling pathway mediates the WGE mode of action. We focused on the downstream effector Nrf2 encoded by cap-n-collar (cnc) in Drosophila. Intriguingly, neither overexpression (UAS-cncC-FL2) nor RNAi knockdown (UAS-cncTRiP) of Nrf2 in dopaminergic neurons had an impact on the climbing ability of Ddc>G2019S flies (Figure 5A). Also, WGE feeding still rescued locomotion of Ddc>G2019S flies with Nrf2 knockdown (Ddc>G2019S; cncTRiP) to levels comparable to WGE-fed control flies (Ddc>G2019S; mCD8-GFP) without Nrf2 knockdown (Figure 5A). Thus, the Nrf2 pathway activation by WGE that appears to be effective in protecting neurons and restoring locomotion is likely exerted in cells other than neurons.
Nrf2 activation may be examined by assessing GFP signal from the ARE-GFP reporter that harbors Nrf2 binding sites and responds to Nrf2 activation (Chatterjee and Bohmann, 2012). ARE-GFP adult fly brains displayed low basal GFP signals (Figure 5—figure supplement 1A), but feeding with 0.1% WGE elicited many GFP-positive signals in Repo-positive glia (arrowheads in Figure 5—figure supplement 1B), evidencing that glia may be the primary cell type in which Nrf2 is activated.
Next, we investigated if WGE-induced Nrf2 activity in glia is effective in promoting locomotion in G2019S flies. To this end, we employed the LexA-LexAop system to overexpress wild-type Lrrk2 or G2019S mutant protein in dopaminergic neurons (Ddc-LexA>Lrrk2 and Ddc-LexA>G2019S, respectively) and used the GAL4 driver to manipulate Nrf2/CncC activity in glia (repo>cncC-FL2 for overexpression and repo>cnc-RNAi for knockdown). As a first step, we validated the age-dependent locomotion decline of Ddc-LexA>G2019S flies that was severer than Ddc-LexA>Lrrk2 and could be rescued by 0.1% WGE feeding (Figure 5—figure supplement 2). We then assayed the climbing ability of Ddc-LexA>G2019S flies exhibiting repo-GAL4-driven Nrf2 overexpression in glia (Ddc-LexA>G2019S; repo>cncC-FL2). Significantly, the Ddc-LexA>G2019S; repo>cncC-FL2 flies performed better in the climbing assay than Ddc-LexA>G2019S; repo-GAL4 without Nrf2 overexpression (Figure 5B). We also performed Nrf2 knockdown in glia (Ddc-LexA>G2019S; repo>cnc-RNAi), which had little impact on the already declined climbing activity in Ddc-LexA>G2019S; repo-GAL4 (Figure 5B). Importantly, WGE feeding could not rescue locomotion deficits of glial Nrf2-knockdown flies (Ddc-LexA>G2019S; repo>cnc-RNAi) (Figure 5B, compare to Ddc-LexA>G2019S; repo-GAL4 with WGE feeding). Thus, glial overexpression of Nrf2 partially restores locomotion of Ddc-LexA>G2019S flies, and glial depletion of Nrf2 abolishes the ability of WGE to rescue impaired locomotion.
We stained dopaminergic neurons of 6-week-old adult fly brains and confirmed that numbers of TH-positive dopaminergic neurons in the PPL1 cluster were reduced in the Ddc-LexA>G2019S; repo-GAL4 flies compared to Ddc-LexA>Lrrk2; repo-GAL4 controls (Figure 5C and D). Importantly, numbers of dopaminergic neurons in the PPL1 cluster were restored upon glial overexpression of Nrf2 in the Ddc-LexA>G2019S; repo>cncC-FL2 flies. In contrast, glial Nrf2 knockdown had little impact on the already reduced dopaminergic neurons. Taken together, these results support that glial Nrf2 is compromised in Ddc-LexA>G2019S flies, and glial expression of Nrf2 protects the dopaminergic neurons.
Although WGE feeding suppressed hyperactivity of G2019S mutant protein (Figure 3D–G), it was not clear if WGE-mediated Nrf2 activation in glia could directly suppress mutant protein hyperactivity in dopaminergic neurons. To test this possibility, we compared the levels of total Lrrk2 protein and phosphorylated Lrrk2 protein in Ddc-LexA>G2019S with and without glial Nrf2 overexpression (Ddc-LexA>G2019S; repo-GAL4 and Ddc-LexA>G2019S; repo>cncC). We observed comparable levels of Lrrk2 and pLrrk2 in the control and Nrf2 overexpression lines (Figure 6—figure supplement 1A–C). Thus, the neuroprotective effects of Nrf2 activity are unlikely to operate through modulation of Lrrk2 levels or activity.
Nrf2 in astrocyte-like and ensheathing glia is the major target of WGE
Five types of glia with different morphologies and functions have been identified in the fly brain (Freeman, 2015). We decided to identify specific subtypes of glia that may mediate the WGE-induced Nrf2 activity endowing neuronal protection, given that dysfunctional astrocytes and microglia have been linked to onset and progression of both sporadic and familial PD (Kam et al., 2020). In Ddc-LexA>G2019S flies, GAL4-driven cnc knockdown in astrocyte-like (Ddc-LexA>G2019S; alrm>cnc-RNAi) or ensheathing glia (Ddc-LexA>G2019S; R56F03>cnc-RNAi) abolished the improved locomotion elicited by WGE treatment, recapitulating the effect of pan-glial Nrf2 knockdown (Ddc-LexA>G2019S; repo>cnc-RNAi) (Figure 6A). This outcome was not observed when we used GAL4 drivers to knock down cnc in cortex (np2222), perineurial (np6293), or subperineurial (moody) glia. We confirmed the involvement of astrocyte-like and ensheathing glia by means of Nrf2 overexpression in astrocyte-like (Ddc-LexA>G2019S; alrm>cncC-FL2) or ensheathing (Ddc-LexA>G2019S; R56F03>cncC-FL2) glia, with both treatments improving the climbing activity of Ddc-LexA>G2019S flies not subjected to WGE feeding (Figure 6B). These analyses indicate that WGE feeding induces Nrf2 activity in astrocyte-like and ensheathing glia, which mitigates the reduced locomotion displayed by G2019S mutant-expressing flies.
Next, we assayed Nrf2-regulated ARE-GFP expression in astrocyte-like and ensheathing glia of flies expressing G2019S mutant protein and subjected to WGE treatment. We focused on the astrocyte-like and ensheathing cells located adjacent to dopaminergic neurons. In control flies expressing wild-type Lrrk2, we detected basal levels of GFP signal in mCherry-positive astrocyte-like (alrm-GAL4) or ensheathing glia (R56F03) (Figure 6C–F). We detected lower levels of GFP signal in these cells when G2019S mutant protein was expressed in dopaminergic neurons. However, upon feeding with 0.1% WGE, we observed higher levels of GFP signal in both types of glia. Changes in the intensities of GFP signals were also detected in TH-positive dopaminergic neurons, although the levels were lower than in glia (Figure 6—figure supplement 2A and B). Thus, overexpression of G2019S mutant protein in dopaminergic neurons elicited reduced Nrf2 signaling activity, but WGE feeding restored or further enhanced Nrf2 activities in these two glial subtypes.
Nrf2 activation antagonizes BMP signaling in glia
G2019S mutant protein in dopaminergic neurons has been shown previously to enhance the expression of the proprotein convertase Furin 1 (Fur1) that processes the BMP signaling molecule Gbb for maturation and release, leading to activation of the BMP signaling pathway in glia (Maksoud et al., 2019). We confirmed that finding by removing one copy of Mad that encodes the pathway’s downstream effector to restore locomotion in Ddc>G2019S flies (Figure 7—figure supplement 1). To address if WGE-induced Nrf2 activation could antagonize BMP signaling activity in glia, first we assessed expression of the phosphorylated Mad (pMad) activated by BMP signaling. In glia of Ddc>G2019S adult fly brains, pMad levels were higher than in Ddc>Lrrk2 brains and they could be suppressed by WGE treatment (Figure 7A and B). Similar to the previous report (Maksoud et al., 2019), glial overexpression of Mad (UAS-Mad) or constitutively active type I receptor Tkv (UAS-tkvQ253D) was sufficient to impair locomotion, even without expressing G2019S mutant protein in neurons. However, impaired locomotion was rescued in both cases by 0.1% WGE feeding (Figure 7C and D). In addition, numbers of dopaminergic neurons in the PPL1 cluster were reduced upon glial overexpression of Mad or tkvQ253D and they were restored by 0.1% WGE treatment (Figure 7E–H). Together, these analyses indicate that activation of BMP signaling in glia recapitulates the phenotypes observed in flies overexpressing G2019S mutant protein in dopaminergic neurons and, furthermore, that these effects can be suppressed by WGE treatment.
Next, we explored if WGE-induced Nrf2 activation antagonizes BMP signaling in glia. Glial Nrf2 overexpression in Ddc-LexA>G2019S flies (Ddc-LexA>G2019S; repo>cncC-FL2) partially rescued the locomotory impairment caused by G2019S mutation (Figure 5B), and removing one copy of Mad (Ddc-LexA>G2019S, Mad+/-; repo>cncC-FL2) further enhanced this effect (Figure 8A). Indeed, this outcome was equivalent to removing one copy of Mad but without Nrf2 overexpression (Ddc-LexA>G2019S, Mad+/-; repo-GAL4), suggesting that Nrf2 functions mainly to antagonize Mad activity (Figure 8A). Consistently, Nrf2 overexpression in glia or WGE treatment suppressed the upregulation of pMad levels in glia caused by G2019S mutant protein overexpression in dopaminergic neurons (Ddc-LexA>G2019S; repo-GAL4) (Figure 8B and C). Depletion of Nrf2 from glia (Ddc-LexA>G2019S; repo>cnc-RNAi), even in the presence of WGE treatment, maintained high pMad levels in glia (Figure 8B and C). Thus, modulation of Nrf2 activity, either by genetic manipulation or by WGE treatment, has an antagonistic effect on pMad levels in glia.
We also addressed if Mad modulates expression of the Nrf2 target ARE-GFP. We observed diminished GFP signal in PPL1-surrounding glia of Ddc-LexA>G2019S adult fly brains relative to the Ddc-LexA>Lrrk2 control. Heterozygosity of Mad in Ddc-LexA>G2019S flies (Ddc-LexA>G2019S, Mad+/-) restored the level of GFP signal, implying that Mad modulates targeted gene expression induced by Nrf2 activity (Figure 8D and E).
The effects of WGE treatment in a LRRK2-G2019S mouse model
To further study the effect of WGE on G2019S mutation-induced neurodegeneration in a mammalian system, we fed LRRK2-G2019S transgenic mice with WGE starting at the age of 8.5 months, that is, prior to onset of impairments in locomotion and dopaminergic neurons (Chou et al., 2014). We quantified three locomotor activities from video-tracking paths of an open-field test, that is, accumulative moving distance, average velocity, and percentage of time moving (Figure 9A and B). At the age of 8.5 months, three groups—non-transgenic (nTg), transgenic LRRK2-G2019S, and WGE-fed transgenic LRRK2-G2019S mice—presented comparable locomotor activities. At 9.5 months of age, the LRRK2-G2019S mice displayed clearly impaired locomotion, which was statistically significant at the age of 11.5 months relative to nTg littermates (Figure 9B), consistent with a previous report (Chen et al., 2012). Importantly, the LRRK2-G2019S mice fed with WGE showed improved locomotion throughout the 3-month treatment period, an outcome that was statistically significant at 11.5 months (Figure 9A and B). WGE treatment also suppressed the centrophobism displayed by LRRK2-G2019S mice (Figure 9A). We also analyzed the gait of these three groups of mice (Figure 9C). Similar to our findings from the open-field test, stride length of LRRK2-G2019S mice was significantly reduced at 11.5 months, but it was restored to the level of nTg mice by WGE feeding (Figure 9C and D). Collectively, these analyses show that WGE feeding is an effective means of restoring G2019S mutation-induced locomotory declines in this mouse model of PD.
Since the nigral-striatal system contributes to locomotor function, we counted the number of TH-positive dopaminergic neurons in the substantia nigra. In comparison to nTg littermates, the number of dopaminergic neurons in 11.5-month-old LRRK2-G2019S mice was significantly reduced, but WGE treatment for 3 months abrogated this loss of dopaminergic neurons (Figure 10A and B). In the Ddc>G2019S Drosophila model, glia mediate the protective effects of WGE on dopaminergic neurons. We found that activated microglia marked by ionized calcium-binding adapter molecule 1 (Iba-1) were increased in the substantia nigra of LRRK2-G2019S mice, but this increase was suppressed by WGE treatment (Figure 10C and D, Figure 10—figure supplement 1). We further analyzed levels of activated LRRK2 (pLRRK2) in lysates isolated from the nigra striatum. Levels of pLRRK2 normalized to LRRK2 signal were higher in LRRK2-G2019S mice than in nTg littermates, but WGE treatment partially abrogated that outcome (Figure 10E and F). Since G2019S mutation enhanced Mad signaling activation in the fly brain (Figure 8B and C), we also tested this scenario in the mouse model. Immunoblots revealed that the ratio of phosphorylated Smad2 to total Smad2 (pSmad2/Smad2) was significantly elevated in LRRK2-G2019S mice, but this increase was suppressed by WGE treatment (Figure 10E and G). Moreover, although the level of pSmad3/Smad3 was not significantly enhanced by G2019S mutant protein expression, it was suppressed by WGE treatment (Figure 10E and G). Thus, WGE feeding suppresses G2019S mutation-induced microglia activation and Smad signaling in the substantia nigra of the LRRK2-G2019S mouse model of PD.
In summary, the potential mechanisms of WGE involved in the modulation of the neuron-glial interaction are proposed (Figure 11). WGE regulates the hyperactivation of G2019S mutant protein in dopaminergic neurons and antagonizes the Mad signaling by activating the Nrf2 pathway in glia. Both actions provide neuroprotection.
Discussion
Motor dysfunction in Drosophila neurodegeneration models has been frequently evaluated by means of negative-geotaxis assay that measures the insect innate response. The assay begins with a sudden external stimulation, which initially inhibits spontaneous locomotion but is followed by climbing behavior. The entire response requires motor circuit coordination and muscle tone regulation, two processes that progressively decline with age and that are impacted by neuropathological insults (Grotewiel et al., 2005). In contrast, the open-arena walking assay allows free exploration without disturbance, representing an assay for locomotor activities that can reveal deficits like bradykinesia (Chen et al., 2014). Our G2019S flies exhibited reduced locomotor activity in both assays. Moreover, the G2019S flies also exhibited centrophobism-like behavior in the open arena (Figure 1D). Centrophobism is indicative of emotional abnormalities, such as anxiety and depression, both of which are often associated with PD (Kulisevsky et al., 2008), and was also displayed by the LRRK2-G2019S mice (Figure 9A). WGE treatment suppresses centrophobism in both fly and mouse PD models and has important implications for tackling major symptoms of PD and even non-PD-related depression, as reported for rodent models (Lin et al., 2018; Lin et al., 2016b). Thus, WGE treatment exerts beneficial effects in both of our PD models.
We found that the 0.1% dosage of WGE is optimal in suppressing age-dependent locomotion decline in G2019S flies, with higher and lower doses being less effective (Figure 1A, Figure 1—figure supplement 1B). Although an inverted U-shaped drug response is common, a plausible explanation for the diminished effectiveness of higher doses is that WGE downregulates the day-time but not night-time locomotor activity of flies (Jo et al., 2017). In mice, higher WGE doses have sleep-promoting effects by activating adenosine A1/A2A receptors in the ventrolateral preoptic area (Zhang et al., 2012). Thus, dosage level is critical to the beneficial effects of GE in both PD models.
Gastrodin and 4-HBA are considered the principal active components in GE (Zhan et al., 2016). Although both compounds can cross the blood-brain barrier (Wu et al., 2017), the capability of gastrodin to do so is relatively poor compared to aglyconic 4-HBA due to the glucose moiety (Lin et al., 2007). Gastrodin is quickly metabolized to 4-HBA and undetermined metabolites in the brain (Lin et al., 2008), perhaps explaining the lower effectiveness of gastrodin in G2019S flies. However, it is likely that the combination of gastrodin with other components in GE might exert optimal beneficial effects.
Expression of G2019S mutant protein impacts different clusters of dopaminergic neurons in the fly brain that are known for their connectivity and function. Activation of two specific mushroom body (MB)-projection dopaminergic neurons in the PPL1 cluster inhibits climbing performance (Sun et al., 2018). Mutations in the circadian gene Clock (Clk) cause PPL1 dopaminergic neuron degeneration, accelerating impaired age-associated climbing ability (Vaccaro et al., 2017). Dopaminergic neurons in the PPL2 cluster extend processes to the calyx of the MB, which has been linked to climbing activity (Sun et al., 2018). In contrast, PPM3 neurons project to the central complex, activation of which enhances locomotion (Kong et al., 2010). A significant reduction in dopaminergic neurons in the PPM1/2 cluster was only found at week 4 when impaired locomotion of G2019S flies was prominent. In a PD fly model involving SNCA overexpression and aux knockdown, dopaminergic neurons in the PPM1/2 cluster were selectively degenerated and this phenotype was accompanied by impaired locomotion in relatively young adult flies (Song et al., 2017). We postulate that the age-dependent impaired locomotion displayed by G2019S flies could be caused by gradual and differential loss of dopaminergic neurons in these clusters, thereby affecting different aspects of locomotion. However, further study is needed to test that hypothesis.
Expression of the G2019S mutant protein induces Lrrk2 auto- and hyperphosphorylation, as well as protein accumulation, together enhancing cellular Lrrk2 activity and causing aberrant downstream signaling (Sheng et al., 2012). We have shown here that neuronal expression of Lrrk2-G2019S reduced Akt phosphorylation (Figure 4A and B). Consistently, hyperactivated G2019S mutant protein impaired interaction with and phosphorylation of Akt, resulting in compromised signaling and accelerated neurodegeneration (Ohta et al., 2011; Panagiotakopoulou et al., 2020). However, WGE feeding restored downstream Akt signaling by suppressing G2019S mutant protein hyperactivation. Rab10, one of the best-characterized substrates for Lrrk2, mediates several of Lrrk2’s cellular functions (Karayel et al., 2020). In Drosophila, Rab10 and Lrrk2-G2019S synergistically affect the activity of dopaminergic neurons, mediating deficits in movement (Fellgett et al., 2021; Petridi et al., 2020). We have shown that WGE treatment downregulated levels of Lrrk2 accumulation and phosphorylated Rab10 (Figure 3F–H), thus alleviating their synergistic toxicity. Several kinase inhibitors have been developed to block the kinase activity of Lrrk2, including of both wild-type Lrrk2 and the G2019S mutant, which could affect endogenous Lrrk2 activity (Sheng et al., 2012). Instead, WGE treatment modulates the phosphorylation status and protein level of the G2019S mutant but not those of wild-type Lrrk2. The new hydrogen bond created at the Ser2019 autophosphorylation site may provide a docking site for some chemicals in WGE, representing a possible explanatory mechanism that warrants further study (Lang et al., 2015).
The antioxidation and detoxification factor Nrf2 is a target of Akt activation. Nrf2 phosphorylation and HO-1 expression levels revealed that Nrf2 is inactivated in G2019S flies, but it was activated by WGE treatment (Figure 4C and D). Intriguingly, our genetic data indicate that Nrf2 primarily functions in the glia of G2019S flies, with Nrf2 depletion from glia eliminating the beneficial effects of WGE and glial Nrf2 activation partially substituting for WGE feeding (Figure 5B). Cortical neurons express much lower levels of Nrf2 than astrocytes owing to hypo-acetylation and transcriptional repression of the Nrf2 promoter (Bell et al., 2015). Moreover, neurons express greater amounts of Cullin 3, the scaffold component of the E3 ubiquitin ligase that targets Nrf2 for proteasomal degradation (Jimenez-Blasco et al., 2015). Both those mechanisms render neuronal Nrf2 inert to activation. Nrf2 activation in astrocytes maintains neuronal integrity and function against oxidative insults in response to stress by supplying antioxidants such as glutathione and HO-1 (Kraft et al., 2004; Vargas and Johnson, 2009). Previous study showed that 4-HBA triggers glia to secrete HO-1 via the Nrf2 pathway, protecting neurons from hydrogen peroxide in the primary culture (Luo et al., 2017). In PD models in which wild-type or mutant α-synuclein is overexpressed, activation of neuronal Nrf2 (Barone et al., 2011; Skibinski et al., 2017) or astrocytic Nrf2 (Gan et al., 2012) proved neuroprotective. In a previous study, lovastatin treatment provides neuroprotection in the G2019S-induced PD model, also through the Akt/Nrf2 pathway (Lin et al., 2016b). As activation of neuronal Nrf2 plays a nonconventional role in promoting developmental dendrite pruning (Chew et al., 2021), it remains interesting to further study the cell types that mediate the action of lovastatin. By genetically manipulating the G2019S fly model, we have shown that WGE-induced Nrf2 activation in glia but not in neurons protects dopaminergic neurons from degeneration and ameliorates impaired locomotion.
Astrocyte-like and ensheathing glia are two major types of glia in the Drosophila nervous system, surrounding and also extending long processes into neuropils of the brain. These astrocyte-like glia exhibit a morphology and function similar to those of mammalian astrocytes, including reuptake of neurotransmitters and phagocytosis of neuronal debris (Freeman, 2015; Tasdemir-Yilmaz and Freeman, 2014). Ensheathing glia of varying morphologies encase axonal tracts and neuropils, regulating neuronal excitability and participating in phagocytosis and injury-induced inflammation (Doherty et al., 2009; Otto et al., 2018). Thus, given their proximity to neurons and similar functions, it is not surprising that both types of glia collectively mediate the protective effects of WGE.
Communication between neurons and glia maintains homeostasis, yet also confers the disease state during neurodegeneration. In the Drosophila G2019S model, upregulation of the BMP ligand Gbb in dopaminergic neurons activates Mad/Smad signaling in glia, which promotes neuronal degeneration via a feedback mechanism (Maksoud et al., 2019). Surprisingly, although the number of dopaminergic neurons in the fly brain is relatively small, the upregulated pMad signal spreads throughout the brain (Figure 7B), suggesting that BMP can be disseminated over long distances. In PD patients, higher levels of TGF-β1 have been detected in the striatum and ventricular cerebrospinal fluid (Vawter et al., 1996). Thus, members of the TGF-β1 superfamily such as TGF-β1 and BMP signaling molecules may represent indicators of neuronal degeneration. Accordingly, disrupting the glia-to-neuron feedback mechanism may sustain neuronal survival. In glia, we found that WGE treatment downregulated the pMad levels that had been increased in the G2019S flies (Figure 7A and B). Nrf2 activation in glia also suppressed the enhanced levels of pMad in G2019S flies (Figure 8B and C). Indeed, our genetic assays indicate that the Nrf2 and Mad pathways interact in the glia of G2019S flies. Thus, WGE exerts its beneficial effects by activating Nrf2 to antagonize the Mad activity that would otherwise contribute to the degeneration of dopaminergic neurons. As a transcriptional activator, Nrf2 induces expression of the inhibitory component Smad7 to form inactive Smad complexes (Song et al., 2019) and the phosphatase subunit PPM1A to alter Smad2/3/4 phosphorylation and DNA binding (Lin et al., 2006). Given that these components are conserved in Drosophila, Nrf2 may employ similar pathways to block Mad signaling in glia.
That glial Nrf2 activation protects neurons is evidenced by our observations of enhanced HO-1 expression (Figure 4C) and increased numbers of dopaminergic neurons (Figure 5C and D). These results support that the role of Nrf2 in glia is to induce expression of antioxidation building blocks, such as phase-II detoxification enzymes, and to enhance inflammatory processes (Hirrlinger and Dringen, 2010; Rojo et al., 2010). In a model of fibrosis, TGF-β/Smad2/3 suppressed expression of the ARE-luciferase reporter and glutathione (Ryoo et al., 2014). Moreover, Nrf2 knockdown was shown to reduce expression of the antioxidative enzyme NAD(P)H quinone dehydrogenase 1 (NQO1), thereby elevating cellular oxidative stress and upregulating TGF-β/Smad targeted gene expression (Prestigiacomo and Suter-Dick, 2018). Hence, we propose that WGE promotes Nrf2 activation to antagonize the Smad signaling in glia that is induced by dopaminergic neuron-secreted BMP/Gbb signal during degeneration.
In our study, the LRRK2-G2019S mice show locomotor defects and dopaminergic loss at the age of 11.5 months. A previous study shows only earlier signs of defects, the reduction of the dopamine level and release at the age of 12 months in the LRRK2-G2019S mice (Li et al., 2010), which could be contributed by the genetic background (FVB/NJ vs. C57BL/6J). Nevertheless, we have demonstrated that feeding these mice with WGE rescues their locomotor coordination, suppresses their centrophobism, and recovers their numbers of dopaminergic neurons and hyperactivated microglia (Figure 9 and Figure 10 A – D). Significantly, we found that the activity of the TGF-β/Smad2/3 pathway was elevated in nigrostriatal brain lysates, and this activity was also suppressed by WGE treatment (Figure 10). Collectively, these results from fly and mouse PD models indicate that the effectiveness of WGE is likely mediated through conserved Nrf2/Mad pathways (Figure 11). Our findings contribute to our mechanistic understanding of PD and provide potential therapeutic strategies that incorporate the traditional herbal medicine GE.
Materials and methods
Drosophila stocks and maintenance
Request a detailed protocolAll fly stocks were maintained on standard cornmeal-based food medium at 25°C. Drosophila stocks sourced from the Bloomington Drosophila Stock Center (Indiana University, Bloomington, USA) were UAS-mCD8-GFP (#5137), elac-GAL4 (#8760), repo-GAL4 (#7215), Ddc-LexA (#54218), UAS-cncTRIP (#25984), Tub-GAL80ts (#7108), alrm-GAL4 (#67032), R56F03-GAL4 (#39157), UAS-tkvQ253D (#36536), and MadK00237 (#10474). NP2222-GAL4 (#112830) was from the Drosophila Genomics Resource Center and NP6293-GAL4 (#105188) was from the Kyoto Stock Center. Other stocks include UAS-Flag-LRRK2-WT (Lin et al., 2010), UAS-Flag-LRRK2-G2019S (Lin et al., 2010), Ddc>GAL4 (Sang et al., 2007), ARE-GFP (Sykiotis and Bohmann, 2008), UAS-cncC-FL2 (Sykiotis and Bohmann, 2008), moody-GAL4 (Bainton et al., 2005), and UAS-Mad (Takaesu et al., 2006). The two LexAop fly lines—LexAop-LRRK2-WT and LexAop-LRRK2-G2019S—were generated in this study. In brief, the cDNAs for LRRK2-WT and LRRK2-G2019S were isolated from the pDEST53-LRRK2-WT and pDEST53-LRRK2-G2019S plasmids (Addgene, MA) for subcloning into LexAop plasmids (Addgene), which were for microinjection (Fly facility, University of Cambridge, UK). The transgenes were site landed at an attP site on the 2nd chromosome (25C6). For temperature-shift assay of GAL80ts flies, parental flies were maintained at 19°C and allowed to mate, before collecting the F1 adults and shifting them to 29°C to inactivate GAL80.
Preparation of WGE and related chemical compounds
Request a detailed protocolAuthentication of GE and preparation of WGE were as described previously (Lin et al., 2018; Lin et al., 2016a). WGE (KO DA Pharmaceutical Co. Ltd., Taoyuan, Taiwan), gastrodin (Wuhan YC Fine Chemical Co., Wuhan, China), and 4-HBA (Sigma-Aldrich, Darmstadt, Germany) were added to freshly prepared cornmeal-based fly food at indicated final concentrations (w/w). For experiments, 1- to 3-day-old post-eclosion flies were collected and transferred to fresh food medium twice per week.
Fly locomotion assay
Request a detailed protocolA negative geotaxis climbing assay was performed to assess locomotor activity, and it was conducted according to a previous study with minor modification (Madabattula et al., 2015). Cohorts of 35 flies from each genotype were assayed weekly for six consecutive weeks. Success rates were calculated as the percentage of flies that could climb above the 8 cm mark of a 20 cm cylinder within 10 s.
The free-walking assay protocol was conducted based on a previous report with minor modification (Chen et al., 2014). Cohorts of eight flies were habituated on a 10 cm agar-filled dish for 30 min. The dishes were gently tapped to encourage the flies to walk, which was video-taped for 5 min. Movement tracks were processed in ImageJ and quantified using the Caltech multiple fly tracker (Ctrax).
Immunostaining and immunoblotting of adult fly brains
Request a detailed protocolThe protocol for immunostaining whole-mount adult brains was essentially as described previously (Lin et al., 2010; Maksoud et al., 2019). Adult fly brains for each genotype were dissected at the indicated timepoints for immunostaining with the following primary antibodies: mouse anti-TH (Immunostar, 22941, 1:1000), mouse anti-repo (Hybridoma Bank DSHB, 8D12, 1:500), chicken anti-GFP (Abcam, ab13970, 1:10,000), and rabbit anti-phospho-Smad3 (Ser423/425) (Abcam, ab52903, 1:250) (Smith et al., 2012). Fluorophore-conjugated secondary antibodies were FITC-conjugated goat anti-mouse IgG (Jackson ImmunoResearch, AB_2338589, 1:500), Alexa Fluor 488-conjugated goat anti-mouse IgG (Invitrogen, A28175, 1:500), Cy3-conjugated goat anti-mouse IgG (Jackson ImmunoResearch, AB_2338680, 1:500), Alexa Fluor 488-conjugated goat anti-rabbit IgG (Invitrogen, A27034, 1:500), and Cy5-conjugated goat anti-rat IgG (Invitrogen, A10525, 1:500). Phalloidin-TRITC (Sigma-Aldrich, P1951, 1:5000) that binds F-actin was also used for counterstaining. Immunofluorescence signals were acquired under confocal microscopy (ZEISS LSM 710, Germany).
Adult brain extracts were prepared according to a previously described protocol (Lin et al., 2010). In brief, ~80 fly heads for each genotype were isolated for extract preparation. Equivalent amounts of samples (30 μg/20 μL/well) were resolved by SDS-PAGE for immunoblotting. The following primary antibodies were used: rabbit anti-human LRRK2 (Abcam, ab133474, 1:1000); rabbit anti-phospho-LRRK2 (Ser1292) (Abcam, ab203181, 1:500); rabbit anti-Akt (Cell Signaling, #4691, 1:1000); rabbit anti-Drosophila phospho-Akt Ser505 (Cell Signaling, #4054, 1:500); rabbit anti-Nrf2 (Thermo Fisher Scientific, 710574, 1:1000); rabbit anti-phospho-Nrf2 (Ser40) (Thermo Fisher Scientific, PA5-67520, 1:1000); mouse anti-HO-1-1 (Thermo Fisher Scientific, MA1-112, 1:1000); rabbit anti-GAPDH (GeneTex, GTX100118, 1:5000); and rabbit anti-alpha tubulin (Cell Signaling, #2144, 1:10,000), followed by blotting with secondary antibodies peroxidase-conjugated goat anti-rabbit IgG (Jackson ImmunoResearch, AB_2307391, 1:7500) and peroxidase-conjugated goat anti-mouse IgG (Jackson ImmunoResearch, AB_10015289, 1:7500).
ARE-GFP reporter assay
Request a detailed protocolThe antioxidant response element (ARE)-GFP reporter assay was a modification of the protocol from a previous study (Sykiotis and Bohmann, 2008). ARE-GFP flies of 1 week old (for Figure 5—figure supplement 1) or 6 weeks old (Figure 6) were fed with regular food or food containing 0.1% WGE prior to brain dissection and GFP immunostaining. To quantify ARE-GFP in the 6-week-old adult fly brain, confocal images were processed in ImageJ. Mean GFP fluorescence intensities in mCherry-labeled glial cells and TH-positive dopaminergic neurons of the PPL1 cluster were quantified and normalized as GFP/mCherry.
Animal care and treatments
Request a detailed protocolTransgenic LRRK2-G2019S mice were purchased from the Jackson Laboratory (JAX stock #009609, Bar Harbor, ME) and maintained at the animal center of the National Taiwan University Hospital (NTUH). Non-transgenic (nTg) and heterozygous transgenic LRRK2-G2019S mice were obtained by crossing heterozygous LRRK2-G2019S mice with wild-type FVB/NJ mice (JAX stock #001800). Mice at 8.5 months old were assigned to one of three groups (5–6 mice per group): nTg, LRRK2-G2019S, and LRRK2-G2019S fed with WGE (0.5 g/kg body weight per day) (Lin et al., 2018) for 3 months.
Behavioral assays
Request a detailed protocolWe employed two behavioral tests to assay mouse motor function, that is, an open-field assay to assess spontaneous locomotor activity and CatWalk XT gait analysis to assay coordination. Behavioral experiments were conducted blind to genotype, as described previously (Lin et al., 2020).
Immunohistochemical staining
Request a detailed protocolAfter 3 months of WGE treatment, mice were sacrificed at the age of 11.5 months. The substantia nigra and striatum were dissected out. The substantia nigra was subjected to immunostaining, as described previously (Lin et al., 2020). Anti-tyrosine hydroxylase (TH) (Millipore, AB152, 1:200) and anti-ionized calcium-binding adapter molecule 1 (Iba-1) (GeneTex, GTX100042, 1:200) were used as primary antibodies for 24 hr at 4°C. Secondary antibodies were DyLight 488 goat anti-rabbit 1:300 and Alexa Fluor 546 goat anti-rabbit at 1:200 (25°C for 1 hr). Mounting medium with DAPI (GeneTex, GTX30920) was used as a counterstain.
Immunoblotting
Request a detailed protocolFrozen nigrostriatal brain tissues were homogenized and mixed with lysis buffer to determine protein content and for immunoblotting, as described previously (Lin et al., 2020). The membrane was incubated overnight at 4°C with the following primary antibodies: anti-LRRK2 (Abcam, ab133474, 1:5000), anti-phospho-LRRK2 (Ser1292, Abcam, ab203181, 1:1000), anti-Smad2 (Cell Signaling, #5339, 1:1000), anti-phospho-Smad2 (Ser465/467, Cell Signaling, #3108, 1:1000), anti-Smad3 (Cell Signaling, #9523, 1:1000), anti-phospho-Smad3 (Ser423/425, Abcam, ab52903, 1:1000), and anti-beta actin (Sigma-Aldrich, A5441, 1:5000). After washing, peroxidase-conjugated goat anti-rabbit IgG (GeneTex, GTX213110-01, 1:5000) or peroxidase-conjugated goat anti-mouse IgG (GeneTex, GTX213111-01, 1:5000) were used as secondary antibodies.
Statistical analysis
Request a detailed protocolAll statistical analyses were carried out in GraphPad Prism 6 (La Jolla, CA). Data are presented as mean ± standard error (SEM). Statistical analysis was performed using either Student t-test or one-way analysis of variance (ANOVA) followed by Tukey’s multiple comparison test. p<0.05 were considered indicative of significance. For exact n numbers, p-values, F-values, t-values, and degrees of freedom of each statistical test, please see the statistical information in Source data 1.
Appendix 1
Data availability
All data generated or analyzed during this study are included in the manuscript and supporting files.
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Article and author information
Author details
Funding
Ministry of Science and Technology, Taiwan (MOST-108-2311-B-001-039-MY3)
- Cheng-Ting Chien
Parkinson's UK (H1201)
- Stavroula Petridi
- Christopher JH Elliott
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Acknowledgements
We thank D Bohmann (URMC), U Heberlein (HHMI), SJ Newfeld (ASU), T-K Sang (NTHU), Bloomington Stock Center, Drosophila Genomics Resource Center, Kyoto Stock Center, and Taiwan Fly Core for providing fly stocks. We thank KO DA Pharmaceutical Co. Ltd. (Taoyuan, Taiwan) and Wuhan YC Fine Chemical Co. (Wuhan, China) for providing WGE and gastrodin, respectively.
Ethics
All animal procedures were approved by the local ethics committee and the Institutional Animal Care and Use Committee (IACUC) of the National Taiwan University (IACUC approval no. 20180103).
Copyright
© 2021, Lin et al.
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
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