Figures and data in A crosstalk between hepcidin and IRE/IRP pathways controls ferroportin expression and determines serum iron levels in mice

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Version of Record published: September 22, 2022 (This version)
Accepted Manuscript published: September 6, 2022 (Go to version)
Accepted: August 30, 2022
Received: June 23, 2022
Preprint posted: November 1, 2021 (Go to version)

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Edouard Charlebois
Carine Fillebeen
Angeliki Katsarou
Aleksandr Rabinovich
Kazimierz Wisniewski
Vivek Venkataramani
Bernhard Michalke
Anastasia Velentza
Kostas Pantopoulos

A crosstalk between hepcidin and IRE/IRP pathways controls ferroportin expression and determines serum iron levels in mice

eLife 11:e81332.

https://doi.org/10.7554/eLife.81332

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Dietary iron loading does not disrupt inflammatory hepcidin induction in LPS-treated wild type mice but blunts hepcidin-mediated hypoferremia.

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Iron overload blunts hepcidin responsiveness to LPS-induced inflammation.

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Effects of dietary iron manipulations in hepatic and splenic iron of wild type and Hjv-/- mice.

Effects of LPS on hepatic and splenic ferroportin of iron-manipulated wild type and Hjv-/- mice.

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Low magnification immunohistochemical images of ferroportin in liver sections of dietary iron-manipulated wild type and Hjv-/- mice following LPS treatment.

Low magnification immunohistochemical images of ferroportin in spleen sections of dietary iron-manipulated wild type and Hjv-/- mice following LPS treatment.

Iron depletion of Hjv-/- mice improves the efficacy of synthetic hepcidin to promote hypoferremia.

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Perls staining for iron deposits in liver and spleen sections of dietary iron-manipulated wild type and Hjv-/- mice following treatment with synthetic hepcidin.

Western analysis of transferrin receptors (Tfr1 and Tfr2) of dietary iron-manipulated wild type and Hjv-/- mice following treatment with synthetic hepcidin.

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Effects of LPS treatment on expression of mRNAs encoding iron transport proteins and signaling endpoints in the liver of dietary iron-manipulated wild type and Hjv-/- mice.

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Effects of synthetic hepcidin on hepatic and splenic ferroportin of iron-manipulatedwild type and Hjv-/- mice.

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Low magnification immunohistochemical images of ferroportin in liver sections of dietary iron-manipulated wild type and Hjv-/- mice following treatment with synthetic hepcidin.

Low magnification immunohistochemical images of ferroportin in spleen sections of dietary iron-manipulated wild type and Hjv-/- mice following treatment with synthetic hepcidin.

Dietary iron manipulations trigger IRP responses in the liver and spleen, as well as in primary hepatocytes and non-parenchymal liver cells of wild type and Hjv-/- mice.

Effects of dietary iron manipulations in hepatic and splenic iron of wild type and Hjv^-/- mice.

Effects of LPS on hepatic and splenic ferroportin of iron-manipulated wild type and Hjv^-/- mice.

Low magnification immunohistochemical images of ferroportin in liver sections of dietary iron-manipulated wild type and Hjv^-/- mice following LPS treatment.

Low magnification immunohistochemical images of ferroportin in spleen sections of dietary iron-manipulated wild type and Hjv^-/- mice following LPS treatment.

Iron depletion of Hjv^-/- mice improves the efficacy of synthetic hepcidin to promote hypoferremia.

Perls staining for iron deposits in liver and spleen sections of dietary iron-manipulated wild type and Hjv^-/- mice following treatment with synthetic hepcidin.

Western analysis of transferrin receptors (Tfr1 and Tfr2) of dietary iron-manipulated wild type and Hjv^-/- mice following treatment with synthetic hepcidin.

Effects of LPS treatment on expression of mRNAs encoding iron transport proteins and signaling endpoints in the liver of dietary iron-manipulated wild type and Hjv^-/- mice.

Effects of synthetic hepcidin on hepatic and splenic ferroportin of iron-manipulatedwild type and Hjv^-/- mice.

Low magnification immunohistochemical images of ferroportin in liver sections of dietary iron-manipulated wild type and Hjv^-/- mice following treatment with synthetic hepcidin.

Low magnification immunohistochemical images of ferroportin in spleen sections of dietary iron-manipulated wild type and Hjv^-/- mice following treatment with synthetic hepcidin.

Dietary iron manipulations trigger IRP responses in the liver and spleen, as well as in primary hepatocytes and non-parenchymal liver cells of wild type and Hjv^-/- mice.