A crosstalk between hepcidin and IRE/IRP pathways controls ferroportin expression and determines serum iron levels in mice

Abstract
Editor's evaluation
Introduction
Results
Discussion
Materials and methods
Data availability
References
Article and author information
Metrics

Abstract

The iron hormone hepcidin is transcriptionally activated by iron or inflammation via distinct, partially overlapping pathways. We addressed how iron affects inflammatory hepcidin levels and the ensuing hypoferremic response. Dietary iron overload did not mitigate hepcidin induction in lipopolysaccharide (LPS)-treated wild type mice but prevented effective inflammatory hypoferremia. Likewise, LPS modestly decreased serum iron in hepcidin-deficient Hjv^-/- mice, model of hemochromatosis. Synthetic hepcidin triggered hypoferremia in control but not iron-loaded wild type animals. Furthermore, it dramatically decreased hepatic and splenic ferroportin in Hjv^-/- mice on standard or iron-deficient diet, but only triggered hypoferremia in the latter. Mechanistically, iron antagonized hepcidin responsiveness by inactivating IRPs in the liver and spleen to stimulate ferroportin mRNA translation. Prolonged LPS treatment eliminated ferroportin mRNA and permitted hepcidin-mediated hypoferremia in iron-loaded mice. Thus, de novo ferroportin synthesis is a critical determinant of serum iron and finetunes hepcidin-dependent functional outcomes. Our data uncover a crosstalk between hepcidin and IRE/IRP systems that controls tissue ferroportin expression and determines serum iron levels. Moreover, they suggest that hepcidin supplementation therapy is more efficient when combined with iron depletion.

Editor's evaluation

The authors present a manuscript aiming to understand how systemic iron overload counteracts the hypoferremic effects of a specific inflammatory stimulus, specifically focused on the role of mechanisms of ferroportin regulation to achieve hypoferremia during inflammation. This work is of interest to the community of researchers interested in the interaction of systemic iron regulation and inflammation and possibly ultimately clinicians managing iron disorders. This work also is of novel significance for translational purposes and could lead to the design of better therapeutics for iron related disorders and/or anemia of chronic inflammation. The current study demonstrates LPS and exogenous hepcidin can synergistically lead to hypoferremia even in iron overload conditions and provides data implicating ferroportin translation in contributing to the fully sequestering iron in cells involved in iron flows to induce hypoferremia.

https://doi.org/10.7554/eLife.81332.sa0

Introduction

Systemic iron balance is controlled by hepcidin, a peptide hormone that is produced by hepatocytes in the liver and operates in target cells by binding to the iron exporter ferroportin (Camaschella et al., 2020; Katsarou and Pantopoulos, 2020). This results in ferroportin internalization and lysosomal degradation but also directly inhibits ferroportin function by occluding its iron export channel (Aschemeyer et al., 2018; Billesbølle et al., 2020). Ferroportin is highly expressed in duodenal enterocytes and tissue macrophages, which are instrumental for dietary iron absorption and iron recycling from senescent erythrocytes, respectively. Ferroportin is also expressed in hepatocytes, where excess iron is stored and can be mobilized on demand. Hepcidin-mediated ferroportin inactivation inhibits iron entry into plasma. This is a critical homeostatic response against iron overload, but also an innate immune response against infection (Ganz and Nemeth, 2015). Thus, hepcidin expression is induced when systemic iron levels are high to prevent dietary iron absorption or under inflammatory conditions to promote iron retention within ferroportin-expressing cells and render the metal unavailable to extracellular pathogens.

The hepcidin-encoding Hamp gene is transcriptionally induced by iron or inflammatory stimuli via BMP/SMAD (Wang and Babitt, 2019) or IL-6/STAT3 (Schmidt, 2015) signaling, respectively. These pathways crosstalk at different levels. For instance, the BMP co-receptor hemojuvelin (HJV), a potent enhancer of iron-dependent BMP/SMAD signaling, is also essential for the inflammatory induction of hepcidin. Thus, Hjv^-/- mice, a model of juvenile hemochromatosis characterized by severe iron overload and hepcidin deficiency (Huang et al., 2005), exhibit blunted inflammatory induction of hepcidin and fail to mount a hypoferremic response following LPS treatment or infection with E. coli (Fillebeen et al., 2018). Excess iron inhibits hepcidin induction via the BMP/SMAD and IL-6/STAT3 signaling pathways in cultured cells (Charlebois and Pantopoulos, 2021; Yu et al., 2021), but the in vivo relevance of these findings is not known.

Hepcidin-dependent inhibition of ferroportin activity and expression is a major but not the sole contributor to inflammatory hypoferremia (Guida et al., 2015; Deschemin and Vaulont, 2013). This is related to the fact that ferroportin expression is regulated by additional transcriptional and post-transcriptional mechanisms (Drakesmith et al., 2015). Thus, ferroportin transcription is induced by iron (Aydemir et al., 2009) and suppressed by inflammatory signals (Ludwiczek et al., 2003), while translation of Slc40a1(+IRE) mRNA, the major ferroportin transcript that harbors an ‘iron responsive element’ (IRE) within its 5’ untranslated regions (5’ UTR) is controlled by ‘iron regulatory proteins’ (IRPs), IRP1 and IRP2. The IRE/IRP system accounts for coordinate post-transcriptional regulation of iron metabolism proteins in cells (Wang and Pantopoulos, 2011; Muckenthaler et al., 2008). In a homeostatic response to iron deficiency, IRPs bind to the IRE within the Slc40a1(+IRE) and ferritin (Fth1 and Ftl1) mRNAs, inhibiting their translation. IRE/IRP interactions do not take place in iron-loaded cells, allowing de novo ferroportin and ferritin synthesis to promote iron efflux and storage, respectively. The impact of the IRE/IRP system on the regulation of tissue ferroportin and serum iron is not well understood.

The aim of this work was to elucidate mechanisms by which systemic iron overload affects hepcidin expression and downstream responses, especially under inflammatory conditions. Utilizing wild type and Hjv^-/- mice, we demonstrate that serum iron levels reflect regulation of ferroportin in the liver and spleen by multiple signals. We further show that effective hepcidin-mediated hypoferremia is antagonized by compensatory mechanisms aiming to prevent cellular iron overload. Our data uncovered a crosstalk between hepcidin and the IRE/IRP system that controls ferroportin expression in the liver and spleen, and thereby determines serum iron levels.

Results

Dietary iron overload does not prevent further inflammatory Hamp mRNA induction in LPS-treated wild type mice, but mitigates hepcidin responsiveness

In an exploratory experiment, wild type mice were subjected to dietary iron loading by feeding a high-iron diet (HID) for short (1 day), intermediate (1 week), or long (5 weeks) time intervals; other animals remained on standard (control) diet. As expected, mice on HID for 1 day manifested maximal increases in serum iron (Figure 1A) and transferrin saturation (Figure 1B). They retained physiological liver iron content (LIC; [Figure 1C]) and serum ferritin (Figure 1D), a reflection of LIC. Serum iron and transferrin saturation plateaued after longer HID intake, while LIC and serum ferritin gradually increased to peak at 5 weeks. The dietary iron loading promoted gradual upregulation of serum hepcidin (Figure 1E) and liver Hamp mRNA (Figure 1F), with highest values at 5 weeks. This could not prevent chronic dietary iron overload, in agreement with earlier findings (Corradini et al., 2011; Daba et al., 2013).

Figure 1

Download asset Open asset

Dietary iron loading does not disrupt inflammatory hepcidin induction in LPS-treated wild type mice but blunts hepcidin-mediated hypoferremia.

Nine-week-old male mice (n=12–14 per group) were fed control diet or high-iron diet (HID) for 1 day, 1 week, or 5 weeks prior to sacrifice. Half of the mice were injected intraperitoneally with saline and the other half with 1 µg/g LPS 4 hr before sacrifice. Sera were collected by cardiac puncture and analyzed for: (A) iron, (B) transferrin saturation, (D) ferritin, and (E) hepcidin. Livers were dissected and processed for biochemical analysis of: (C) liver iron content (LIC) by the ferrozine assay and (F) *Hamp* mRNA by qPCR. The dotted line in (A) and (B) indicates baseline serum iron and transferrin saturation, respectively, from mice on control diet. Data (**A–E**) are presented as the mean ± SEM and in (F) as geometric mean ± SD. Statistically significant differences (p<0.05) over time compared to values from saline- or LPS-treated control mice are indicated by a or b, respectively.

Figure 1—source data 1 qPCR data.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig1-data1-v2.xlsx
Download elife-81332-fig1-data1-v2.xlsx
Figure 1—source data 2 Serum hepcidin calculations.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig1-data2-v2.xls
Download elife-81332-fig1-data2-v2.xls
Figure 1—source data 3 Liver iron quantification.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig1-data3-v2.xlsx
Download elife-81332-fig1-data3-v2.xlsx
Figure 1—source data 4 Serum iron and transferrin saturation values.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig1-data4-v2.xlsx
Download elife-81332-fig1-data4-v2.xlsx

LPS triggered appropriate hepcidin induction and a robust hypoferremic response in control mice. Interestingly, LPS-induced inflammation resulted in further proportional increase in hepcidin and Hamp mRNA in dietary iron-loaded mice (Figure 1E–F). This was accompanied by significant drops in serum iron and transferrin saturation (Figure 1A–B). However, values did not reach the nadir of LPS-treated control animals and were increasing in mice on HID for longer periods, despite significant hepcidin accumulation. These data suggest that hepatic iron overload does not prevent inflammatory induction of hepcidin; however, it impairs its capacity to decrease serum iron.

Uncoupling inflammatory hepcidin induction from hypoferremic response in wild type and Hjv^-/- mice following dietary iron manipulations

To further explore the potential of hepcidin to promote hypoferremia under iron overload, wild type and Hjv^-/- mice, a model of hemochromatosis, were subjected to dietary iron manipulations. Wild type mice were fed control diet or HID, and Hjv^-/- mice were fed control diet or an iron-deficient diet (IDD) for 5 weeks, to achieve a broad spectrum of hepcidin regulation. Wild type mice on HID and Hjv^-/- mice on control diet or IDD manifested similarly high serum iron and transferrin saturation (Figure 2A–B). Serum non-transferrin bound iron (NTBI) levels appeared modestly elevated in the dietary and genetic iron overload models and seemed to decrease in Hjv^-/- mice following IDD intake (Figure 2C). LIC was substantially reduced in Hjv^-/- mice in response to IDD but also compared to wildtype mice on HID (Figure 2D). The quantitative LIC data were corroborated histologically by Perls staining (Figure 2E and Figure 2—figure supplement 1A). Dietary iron loading increased splenic iron in wild type mice and confirmed that Hjv^-/- mice fail to retain iron in splenic macrophages (Figure 2—figure supplement 1B). As expected, serum hepcidin (Figure 2F) and liver Hamp mRNA (Figure 2G) were maximally induced in HID-fed wild type mice and were low in Hjv^-/- mice on control diet, and further suppressed to undetectable levels following IDD intake.

Figure 2 with 1 supplement see all

Download asset Open asset

Iron overload blunts hepcidin responsiveness to LPS-induced inflammation.

Four-week-old male wild type mice (n=12–14 per group) were placed on high-iron diet (HID) for 5 weeks. Conversely, age- and sex-matched isogenic *Hjv^-/-* mice (n=12–14 per group) were placed on iron-deficient diet (IDD) for 5 weeks to prevent excessive iron overload. Other animals from both genotypes were kept on control diet. Half of the mice were injected with saline and the other half with 1 µg/g LPS; all animals were sacrificed 4 hr later. Sera were collected by cardiac puncture and analyzed for: (A) iron, (B) transferrin saturation, (C) non-transferrin bound iron (NTBI), and (F) hepcidin. Livers were dissected and processed for LIC quantification by the ferrozine assay (D) and for histological detection of iron deposits by Perls’ staining (E; magnification: 20 ×). Livers were also used for qPCR analysis of following mRNAs: (G) *Hamp*, (H) *Id1*, (I) *Socs3*, (J) *Slc40a1(+IRE*), (K) *Slc40a1(-IRE*), (L) *Slc11a2*, (M) *Slc39a14*, (N) *Lcn2,* and (O) *Tfrc*. The dotted line in (A) and (B) indicates baseline serum iron and transferrin saturation, respectively, of wild type mice on control diet. Values in (A) represent ratios of serum iron levels between untreated and LPS-treated mice. Data in (**A–F**) are presented as the mean ± SEM while in (**G–O**) are presented as geometric mean ± SD. Statistically significant differences (p<0.05) compared to values from saline- or LPS-treated wild type control mice are indicated by a or b, respectively.

Figure 2—source data 1 qPCR data.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig2-data1-v2.xlsx
Download elife-81332-fig2-data1-v2.xlsx
Figure 2—source data 2 Serum NTBI calculations.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig2-data2-v2.xlsx
Download elife-81332-fig2-data2-v2.xlsx
Figure 2—source data 3 Serum iron ratios.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig2-data3-v2.xlsx
Download elife-81332-fig2-data3-v2.xlsx
Figure 2—source data 4 Serum hepcidin calculations.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig2-data4-v2.xls
Download elife-81332-fig2-data4-v2.xls
Figure 2—source data 5 Liver iron quantification.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig2-data5-v2.xlsx
Download elife-81332-fig2-data5-v2.xlsx
Figure 2—source data 6 Serum iron and transferrin saturation values.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig2-data6-v2.xlsx
Download elife-81332-fig2-data6-v2.xlsx

LPS reduced serum iron and transferrin saturation in hyperferremic wild type mice on HID and Hjv^-/- mice on control diet or IDD, but not below the baseline of wild type mice on control diet, the only animals that developed a robust hypoferremic response (Figure 2A–B); see also ratios of serum iron levels between untreated and LPS-treated mice in Figure 2A. The LPS treatment was associated with significant accumulation of hepcidin (Figure 2F) and induction of Hamp mRNA (Figure 2G) in all experimental groups, while NTBI (Figure 2C) and LIC (Figure 2D) were unaffected. Notably, LPS-treated wild type mice on HID and Hjv^-/- mice on IDD exhibited dramatic differences in Hamp mRNA but similar blunted hypoferremic responses to the acute inflammatory stimulus. Thus, the profound hepcidin induction in iron-loaded wild type mice cannot decrease serum iron below that of iron-depleted Hjv^-/- mice with negligible hepcidin, which indicates reduced hepcidin responsiveness. In support of this interpretation, Id1 and Socs3 mRNAs (Figure 2H–I), which are markers of BMP/SMAD and IL-6/STAT3 signaling, respectively, were appropriately induced by dietary iron loading or LPS treatment in wild type mice. Thus, the major hepcidin signaling pathways were intact under these experimental conditions.

Serum iron levels are also controlled by hepcidin-independent mechanisms (Guida et al., 2015; Deschemin and Vaulont, 2013). To explore their possible contribution in our experimental setting, we analyzed expression of genes involved in iron transport in the liver, an organ that contributes to iron sequestration during inflammation. Ferroportin is encoded by two alternatively spliced transcripts, Slc40a1(+IRE) and Slc40a1(-IRE) (Zhang et al., 2009). Both of them were significantly increased in the liver of iron-loaded wild type mice on HID and Hjv^-/- mice on control diet, which is consistent with transcriptional induction (Aydemir et al., 2009), and were strongly suppressed by LPS (Figure 2J–K). The LPS treatment induced Slc11a2, Slc39a14 and Lcn2 mRNAs in all animals (Figure 2L–N). These encode the divalent metal transporter DMT1, the NTBI transporter Zip14 and the siderophore-binding protein Lcn2, respectively; Lcn2 mRNA induction was dramatic. The transferrin receptor 1 (Tfr1)-encoding Tfrc mRNA was largely unaffected by LPS, except for a reduction in Hjv^-/- mice on IDD (Figure 2O). The above data indicate that LPS-induced inflammation triggers transcriptional responses favoring reduced iron efflux from the liver and increased uptake of NTBI by liver cells.

To assess the downstream function of hepcidin, we analyzed tissue ferroportin levels. Immunohistochemical staining of liver sections revealed strong ferroportin expression in Kupffer cells, predominantly in periportal areas, under all experimental conditions (Figure 3A and Figure 3—figure supplement 1). Hepatocellular ferroportin staining is also evident in the iron overload models, mostly in periportal hepatocytes (Figure 3—figure supplement 1), and in line with recent data (Katsarou et al., 2021). LPS triggered redistribution and decreased expression of ferroportin in Kupffer cells from wild type but not Hjv^-/- mice (Figure 3—figure supplement 1), as reported in Fillebeen et al., 2018.

Figure 3 with 2 supplements see all

Download asset Open asset

Effects of LPS on hepatic and splenic ferroportin of iron-manipulated wild type and *Hjv^-/-* mice.

Livers and spleens from mice described in Figure 2 were dissected and processed for immunohistochemical and biochemical analysis of ferroportin. Immunohistochemical staining of ferroportin in liver (A) and spleen (C) sections (magnification for liver is 20 × and for spleen 5 ×). Western blot for ferroportin and β-actin in liver (B) and spleen (D) extracts from four representative mice in each condition. Blots were quantified by densitometry and ferroportin/β-actin ratios are shown on the right. Densitometric data are presented as the mean ± SEM. Statistically significant differences (p<0.05) compared to values from saline- or LPS-treated wild type control mice are indicated by a or b, respectively. Statistics in bold were performed using unpaired Student’s t test. HID: high-iron diet; IDD: iron-deficient diet.

Figure 3—source data 1 Western blot quantifications.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig3-data1-v2.xlsx
Download elife-81332-fig3-data1-v2.xlsx
Figure 3—source data 2 Raw unlabeled Western blot ferroportin Figure 3D (a).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig3-data2-v2.jpg
Download elife-81332-fig3-data2-v2.jpg
Figure 3—source data 3 Raw unlabeled Western blot ferroportin Figure 3D (b).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig3-data3-v2.jpg
Download elife-81332-fig3-data3-v2.jpg
Figure 3—source data 4 Raw unlabeled Western blot β-actin Figure 3D.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig3-data4-v2.jpg
Download elife-81332-fig3-data4-v2.jpg
Figure 3—source data 5 Raw unlabeled Western blot β-actin Figure 3D (b).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig3-data5-v2.jpg
Download elife-81332-fig3-data5-v2.jpg
Figure 3—source data 6 Raw unlabeled Western blot ferroportin Figure 3B (a).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig3-data6-v2.jpg
Download elife-81332-fig3-data6-v2.jpg
Figure 3—source data 7 Raw unlabeled Western blot β-actin Figure 3B (a).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig3-data7-v2.jpg
Download elife-81332-fig3-data7-v2.jpg
Figure 3—source data 8 Raw unlabeled Western blot ferroportin and β-actin Figure 3B (a).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig3-data8-v2.jpg
Download elife-81332-fig3-data8-v2.jpg
Figure 3—source data 9 Raw unlabeled Western blot ferroportin Figure 3B (b).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig3-data9-v2.jpg
Download elife-81332-fig3-data9-v2.jpg
Figure 3—source data 10 Raw unlabeled Western blot β-actin Figure 3B (b).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig3-data10-v2.jpg
Download elife-81332-fig3-data10-v2.jpg
Figure 3—source data 11 Raw labeled Western blot β-actin Figure 3B (b).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig3-data11-v2.bmp
Download elife-81332-fig3-data11-v2.bmp
Figure 3—source data 12 Raw labeled Western blot ferroportin Figure 3B (b).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig3-data12-v2.bmp
Download elife-81332-fig3-data12-v2.bmp
Figure 3—source data 13 Raw labeled Western blot ferroportin and β-actin Figure 3B (a).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig3-data13-v2.bmp
Download elife-81332-fig3-data13-v2.bmp
Figure 3—source data 14 Raw labeled Western blot ferroportin Figure 3B (a).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig3-data14-v2.bmp
Download elife-81332-fig3-data14-v2.bmp
Figure 3—source data 15 Raw labeled Western blot β-actin Figure 3B (a).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig3-data15-v2.bmp
Download elife-81332-fig3-data15-v2.bmp
Figure 3—source data 16 Raw labeled Western blot ferroportin Figure 3D (b).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig3-data16-v2.bmp
Download elife-81332-fig3-data16-v2.bmp
Figure 3—source data 17 Raw labeled Western blot ferroportin Figure 3D (a).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig3-data17-v2.bmp
Download elife-81332-fig3-data17-v2.bmp
Figure 3—source data 18 Raw labeled Western blot β-actin Figure 3D (b).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig3-data18-v2.bmp
Download elife-81332-fig3-data18-v2.bmp
Figure 3—source data 19 Raw labeled Western blot β-actin Figure 3D (a).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig3-data19-v2.bmp
Download elife-81332-fig3-data19-v2.bmp

We further analyzed ferroportin in liver homogenates by Western blotting. Levels of biochemically detectable liver ferroportin differed substantially between wild type and Hjv^-/- mice. Thus, they were relatively low in the former and highly induced in the latter (Figure 3B), independently of iron load. The differences were more dramatic compared to those observed by immunohistochemistry (Figure 3A and Figure 3—figure supplement 1). Conceivably, the strong ferroportin signal in Western blots from Hjv^-/- liver homogenates reflects high ferroportin expression in hepatocytes, which are the predominant cell population and make up ~80% of the liver cell mass (Schulze et al., 2019). Yet, hepatocellular ferroportin is less visible by immunohistochemistry because the signal is substantially weaker compared to that in Kupffer cells (see also Figure 6E). Interestingly, the LPS treatment visibly suppressed total liver ferroportin in Hjv^-/- mice on control diet but not IDD, and appeared to modestly reduce it in wild type mice (Figure 3B); albeit without statistical significance. These data are consistent with negative regulation of ferroportin by residual LPS-induced hepcidin in Hjv^-/- mice on control diet, which could explain the small drop in serum iron and transferrin saturation under these acute inflammatory conditions, as reported in Fillebeen et al., 2018. However, liver ferroportin remained detectable and apparently functional, as it did not allow significant iron sequestration and dramatic drop in serum iron. Notably, persistence of relatively high serum iron is also evident in LPS-treated wild type mice on HID, despite maximal hepcidin and minimal liver ferroportin levels.

Next, we analyzed ferroportin in the spleen, an organ with erythrophagocytic macrophages that plays an important role in body iron traffic (Kurotaki et al., 2015). Immunohistochemical analysis shows that LPS reduced ferroportin in red pulp splenic macrophages from wild type mice on control diet, but this effect was less evident in wild type mice on HID and in Hjv^-/- mice on control diet or IDD (Figure 3C and Figure 3—figure supplement 2). Western blot analysis shows a stronger ferroportin signal in splenic extracts from Hjv^-/- animals (Figure 3D), consistent with immunohistochemistry. However, in this assay, LPS suppressed splenic ferroportin in wild type animals and in Hjv^-/- mice on control diet, but not IDD. This could be a result of residual hepcidin upregulation (Figure 2F–G), while the lack of significant splenic ferroportin suppression in Hjv^-/- mice on IDD may denote hepcidin insufficiency. In any case, the relatively high circulating iron levels in dietary iron-loaded and LPS-treated wild type mice indicate continuous iron efflux to plasma despite hepcidin excess.

Insufficient hepcidin leads to blunted hypoferremic response in iron overload

We used human synthetic hepcidin to address whether the failure of mouse models of iron overload to mount an appropriate hypoferremic response to acute inflammation is caused by endogenous hepcidin insufficiency or other mechanisms. Wild type and Hjv^-/- mice subjected to dietary iron manipulations received 2.5 μg/g synthetic hepcidin every two hours for a total of four intraperitoneal injections. Each dose corresponds to ~200-fold excess over endogenous circulating hepcidin in wild type animals. The treatment caused hypoferremia in wild type mice on control diet but not on HID, where the decrease in serum iron was significant but well above baseline of untreated wild type controls (Figure 4A–B); see also ratios of serum iron levels between untreated and hepcidin-treated mice in Figure 4A. Likewise, synthetic hepcidin significantly decreased serum iron but failed to cause dramatic hypoferremia in hepcidin-deficient Hjv^-/- mice on control diet. Notably, hepcidin administration was much more effective in relatively iron-depleted Hjv^-/- mice on IDD, and lowered serum iron and transferrin saturation below baseline. The treatments significantly reduced NTBI in Hjv^-/- mice on control diet, with a trend in mice on IDD (Figure 4C) but did not affect LIC or splenic iron content (SIC) under any experimental conditions (Figure 4D–E and Figure 4—figure supplement 1). Serum iron represents <2% of total tissue iron and, therefore, its acute fluctuations are not expected to dramatically alter LIC or SIC.

Figure 4 with 3 supplements see all

Download asset Open asset

Iron depletion of *Hjv^-/-* mice improves the efficacy of synthetic hepcidin to promote hypoferremia.

Four-week-old wild type male mice (n=12–14 per group) were placed on HID for 5 weeks. Conversely, age- and sex-matched isogenic *Hjv^-/-* mice (n=12–14 per group) were placed on IDD for 5 weeks to prevent excessive iron overload. Other animals from both genotypes were kept on standard diet. Half of the mice were injected every 2 hr for a total of 4 injections with saline, and the other half with 2.5 µg/g synthetic hepcidin. Sera were collected by cardiac puncture and analyzed for: (A) iron, (B) transferrin saturation, and (C) non-transferrin bound iron (NTBI). Livers and spleens were dissected and processed for analysis of: (D) liver iron content (LIC) and (E) splenic iron content (SIC) by the ferrozine assay. (F) qPCR analysis of liver *Hamp* mRNA. The dotted line in (A) and (B) indicates baseline serum iron and transferrin saturation, respectively, of wild type mice on control diet. Values in (A) represent ratios of serum iron levels between untreated and hepcidin-treated mice. Data in (**A–E**) are presented as the mean ± SEM and in (F) as geometric mean ± SD. Statistically significant differences (p<0.05) compared to values from saline- or hepcidin-treated wild type control mice are indicated by a or b, respectively. HID: high-iron diet; IDD: iron-deficient diet.

Figure 4—source data 1 qPCR data.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig4-data1-v2.xlsx
Download elife-81332-fig4-data1-v2.xlsx
Figure 4—source data 2 Liver and spleen iron quantification.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig4-data2-v2.xlsx
Download elife-81332-fig4-data2-v2.xlsx
Figure 4—source data 3 Serum NTBI calculations.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig4-data3-v2.xlsx
Download elife-81332-fig4-data3-v2.xlsx
Figure 4—source data 4 Serum hepcidin calculations.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig4-data4-v2.xls
Download elife-81332-fig4-data4-v2.xls
Figure 4—source data 5 Serum iron ratios.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig4-data5-v2.xlsx
Download elife-81332-fig4-data5-v2.xlsx
Figure 4—source data 6 Serum iron and transferrin saturation values.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig4-data6-v2.xlsx
Download elife-81332-fig4-data6-v2.xlsx

Synthetic hepcidin led to a significant reduction of endogenous Hamp mRNA in wild type mice on control diet (Figure 4F), as earlier reported (Laftah et al., 2004). Conceivably, this is related to destabilization of the Hamp inducer transferrin receptor 2 (Tfr2) in the liver (Figure 4—figure supplement 2), a known response to hypoferremia (Johnson and Enns, 2004). Synthetic hepcidin did not promote inflammation, iron perturbations or alterations in BMP/SMAD signaling in the liver, as judged by the unaltered expression of hepatic Slc40a1(+IRE), Socs3, Id1, and Bmp6 mRNAs (Figure 4—figure supplement 3A-D). Moreover, synthetic hepcidin did not affect Slc11a2, Slc39a14, Lcn2, or Tfrc mRNAs (Figure 4—figure supplement 3E-H), which encode iron transporters; Slc39a14 and Lcn2 are also inflammatory markers.

Next, we analyzed liver ferroportin by immunohistochemistry. Figure 5A and Figure 5—figure supplement 1 show that exogenous hepcidin decreased ferroportin signal intensity in all animal groups to varying degrees. The hepcidin effect was particularly noticeable in Hjv^-/- hepatocytes (see low magnification images in Figure 5—figure supplement 1). Kupffer cells seemed to retain some ferroportin in all groups except Hjv^-/- mice on IDD. Interestingly, while synthetic hepcidin decreased ferroportin signal intensity in Kupffer cells, it did not alter intracellular ferroportin distribution as would be expected based on the data in LPS-treated wild type mice (Figure 5A).

Figure 5 with 2 supplements see all

Download asset Open asset

Effects of synthetic hepcidin on hepatic and splenic ferroportin of iron-manipulatedwild type and *Hjv^-/-* mice.

Livers and spleens from mice described in Figure 4 were dissected and processed for immunohistochemical and biochemical analysis of ferroportin. Immunohistochemical staining of ferroportin in liver (A) and spleen (C) sections (magnification for liver is 20 × and for spleen 10 ×). Western blot for ferroportin and β-actin in liver (B) and spleen (D) extracts from four representative mice in each condition. Blots were quantified by densitometry and ferroportin/β-actin ratios are shown on the right. Densitometric data are presented as the mean ± SEM. Statistically significant differences (p<0.05) compared to values from saline- or hepcidin-treatedwild type control mice are indicated by a or b, respectively. HID: high-iron diet; IDD: iron-deficient diet.

Figure 5—source data 1 Western blot quantifications.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig5-data1-v2.xlsx
Download elife-81332-fig5-data1-v2.xlsx
Figure 5—source data 2 Raw unlabeled Western blot ferroportin Figure 5D (a).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig5-data2-v2.jpg
Download elife-81332-fig5-data2-v2.jpg
Figure 5—source data 3 Raw unlabeled Western blot β-actin Figure 5D (a).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig5-data3-v2.jpg
Download elife-81332-fig5-data3-v2.jpg
Figure 5—source data 4 Raw unlabeled Western blot ferroportin Figure 5B (a).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig5-data4-v2.jpg
Download elife-81332-fig5-data4-v2.jpg
Figure 5—source data 5 Raw unlabeled Western blot ferroportin Figure 5B (b).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig5-data5-v2.jpg
Download elife-81332-fig5-data5-v2.jpg
Figure 5—source data 6 Raw unlabeled Western blot ferroportin Figure 5D (b).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig5-data6-v2.jpg
Download elife-81332-fig5-data6-v2.jpg
Figure 5—source data 7 Raw unlabeled Western blot.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig5-data7-v2.jpg
Download elife-81332-fig5-data7-v2.jpg
Figure 5—source data 8 Raw unlabeled Western blot ferroportin Figure 5D (c).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig5-data8-v2.jpg
Download elife-81332-fig5-data8-v2.jpg
Figure 5—source data 9 Raw unlabeled Western blot β-actin Figure 5D (c).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig5-data9-v2.jpg
Download elife-81332-fig5-data9-v2.jpg
Figure 5—source data 10 Raw unlabeled Western blot β-actin Figure 5B (a).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig5-data10-v2.jpg
Download elife-81332-fig5-data10-v2.jpg
Figure 5—source data 11 Raw unlabeled Western blot β-actin Figure 5B (b).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig5-data11-v2.jpg
Download elife-81332-fig5-data11-v2.jpg
Figure 5—source data 12 Raw labeled Western blot β-actin Figure 5B (b).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig5-data12-v2.bmp
Download elife-81332-fig5-data12-v2.bmp
Figure 5—source data 13 Raw labeled Western blot β-actin Figure 5B (a).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig5-data13-v2.bmp
Download elife-81332-fig5-data13-v2.bmp
Figure 5—source data 14 Raw labeled Western blot ferroportin Figure 5B (b).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig5-data14-v2.bmp
Download elife-81332-fig5-data14-v2.bmp
Figure 5—source data 15 Raw labeled Western blot ferroportin Figure 5B (a).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig5-data15-v2.bmp
Download elife-81332-fig5-data15-v2.bmp
Figure 5—source data 16 Raw labeled Western blot β-actin Figure 5D (a).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig5-data16-v2.bmp
Download elife-81332-fig5-data16-v2.bmp
Figure 5—source data 17 Raw labeled Western blot ferroportin Figure 5D (a).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig5-data17-v2.bmp
Download elife-81332-fig5-data17-v2.bmp
Figure 5—source data 18 Raw labeled Western blot β-actin Figure 5D (c).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig5-data18-v2.bmp
Download elife-81332-fig5-data18-v2.bmp
Figure 5—source data 19 Raw labeled Western blot ferroportin Figure 5D (c).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig5-data19-v2.bmp
Download elife-81332-fig5-data19-v2.bmp
Figure 5—source data 20 Raw labeled Western blot β-actin Figure 5D (b).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig5-data20-v2.bmp
Download elife-81332-fig5-data20-v2.bmp
Figure 5—source data 21 Raw labeled Western blot ferroportin Figure 5D (b).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig5-data21-v2.bmp
Download elife-81332-fig5-data21-v2.bmp
Figure 5—source data 22 Raw labeled Western blot ferroportin and β-actin Figure 5D (a).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig5-data22-v2.bmp
Download elife-81332-fig5-data22-v2.bmp
Figure 5—source data 23 Raw unlabeled Western blot ferroportin and β-actin Figure 5D (a).: https://cdn.elifesciences.org/articles/81332/elife-81332-fig5-data23-v2.jpg
Download elife-81332-fig5-data23-v2.jpg

Western blotting confirmed that total liver ferroportin is highly induced in Hjv^-/- mice (Figure 5B). Again, the signal intensity can be attributed to proteins expressed in hepatocytes. The treatment with synthetic hepcidin did not significantly affect liver ferroportin in wild type mice (either on control diet or HID), but substantially reduced it in Hjv^-/- mice, to almost wild type levels. The effect appeared more pronounced in Hjv^-/- mice on IDD; nevertheless, ferroportin remained detectable.

Splenic ferroportin was reduced in all animal groups following hepcidin treatment, with stronger effects visualized by immunohistochemistry in wild type mice on control diet and Hjv^-/- mice on IDD (Figure 5C and Figure 5—figure supplement 2). At the biochemical level, ferroportin expression was again much stronger in the spleen of Hjv^-/- mice (Figure 5D). Synthetic hepcidin did not significantly affect splenic ferroportin in wild type mice but dramatically reduced it in all Hjv^-/- mice.

Taken together, our data suggest that synthetic hepcidin overcomes endogenous hepcidin deficiency in Hjv^-/- mice. However, it only triggers hypoferremia in these animals following relative iron depletion. On the other hand, in iron-loaded wild type mice with already high endogenous hepcidin, excess synthetic hepcidin fails to promote hypoferremia.

Dietary iron manipulations are sensed by IRPs in the liver and spleen of wild type and Hjv^-/- mice

The IRE/IRP system orchestrates homeostatic adaptation to cellular iron supply (Wang and Pantopoulos, 2011; Muckenthaler et al., 2008). To evaluate the responses of IRPs in the whole liver and spleen to the above-described dietary iron manipulations, we analyzed tissue extracts from wild type and Hjv^-/- mice by an electrophoretic mobility shift assay (EMSA) using a ³²P-labelled IRE probe. The data in Figure 6A–B show that HID intake tended to decrease the IRE-binding activities of IRP1 and IRP2 in both the liver and spleen of wild type mice (statistical significance is only reached in the liver); densitometric quantification of IRE/IRP1 and IRE/IRP2 complexes is shown on the right. Conversely, IDD intake significantly induced the IRE-binding activity of IRP2 in the liver and spleen of Hjv^-/- mice, leaving IRP1 largely unaffected. IRE/IRP2 interactions are better visible in longer exposures (middle panels). EMSAs with tissue extracts previously treated with 2-mercaptoethanol (2-ME) were performed as loading controls (Fillebeen et al., 2014) and are shown in the bottom panels.

Figure 6

Download asset Open asset

Dietary iron manipulations trigger IRP responses in the liver and spleen, as well as in primary hepatocytes and non-parenchymal liver cells of wild type and *Hjv^-/-* mice.

Whole liver (A), whole spleen (B), isolated hepatocytes (C) or isolated non-parenchymal liver cells (D) from the mice described in Figure 4 were analyzed for IRE-binding activity by EMSA with a ³²P-labelled IRE probe in the absence (top) or presence (bottom) of 2% mercaptoethanol (2-ME). Two or three representative samples from each condition are shown. The positions of IRE/IRP1 and IRE/IRP2 complexes are indicated by arrows. Shorter and longer exposures of the autoradiograms are shown in the left and middle panels, respectively. Relative band intensities were quantified by densitometry and shown on the right panels. (E) Isolated hepatocytes and isolated non-parenchymal liver cells were analyzed by Western blotting for expression of ferroportin and β-actin. Blots were quantified by densitometry and ferroportin/β-actin ratios are shown on the right. Densitometric data are presented as the mean ± SEM. Statistically significant differences (p<0.05) in values from wild type mice on control diet are indicated by a, from wild type mice on HID by b, and from *Hjv^-/-* mice on control diet by c. HID: high-iron diet; IDD: iron-deficient diet; IRE: iron-responsive element; IRP: iron regulatory protein; EMSA: electrophoretic mobility shift assay.

Figure 6—source data 1 EMSA quantification.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data1-v2.xlsx
Download elife-81332-fig6-data1-v2.xlsx
Figure 6—source data 2 Western quantification.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data2-v2.xlsx
Download elife-81332-fig6-data2-v2.xlsx
Figure 6—source data 3 Raw unlabeled liver EMSA long exposure Figure 6A.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data3-v2.jpg
Download elife-81332-fig6-data3-v2.jpg
Figure 6—source data 4 Raw unlabeled liver EMSA long exposure 2-ME Figure 6A.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data4-v2.jpg
Download elife-81332-fig6-data4-v2.jpg
Figure 6—source data 5 Raw unlabeled liver EMSA short exposure Figure 6A.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data5-v2.jpg
Download elife-81332-fig6-data5-v2.jpg
Figure 6—source data 6 Raw unlabeled liver EMSA short exposure 2-ME Figure 6A.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data6-v2.jpg
Download elife-81332-fig6-data6-v2.jpg
Figure 6—source data 7 Raw unlabeled spleen EMSA long exposure Figure 6B.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data7-v2.jpg
Download elife-81332-fig6-data7-v2.jpg
Figure 6—source data 8 Raw unlabeled spleen EMSA long exposure 2-ME Figure 6B.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data8-v2.jpg
Download elife-81332-fig6-data8-v2.jpg
Figure 6—source data 9 Raw unlabeled spleen EMSA short exposure Figure 6B.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data9-v2.jpg
Download elife-81332-fig6-data9-v2.jpg
Figure 6—source data 10 Raw unlabeled spleen EMSA short exposure BME Figure 6B.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data10-v2.jpg
Download elife-81332-fig6-data10-v2.jpg
Figure 6—source data 11 Raw unlabeled Western blot β-actin Figure 6E.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data11-v2.jpg
Download elife-81332-fig6-data11-v2.jpg
Figure 6—source data 12 Raw unlabeled Western blot ferroportin Figure 6E.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data12-v2.jpg
Download elife-81332-fig6-data12-v2.jpg
Figure 6—source data 13 Raw unlabeled hepatocytes EMSA short exposure Figure 6C.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data13-v2.jpg
Download elife-81332-fig6-data13-v2.jpg
Figure 6—source data 14 Raw unlabeled hepatocytes EMSA short exposure 2-ME Figure 6C.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data14-v2.jpg
Download elife-81332-fig6-data14-v2.jpg
Figure 6—source data 15 Raw unlabeled hepatocytes EMSA long exposure Figure 6C.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data15-v2.jpg
Download elife-81332-fig6-data15-v2.jpg
Figure 6—source data 16 Raw unlabeled hepatocytes EMSA long exposure 2-ME Figure 6C.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data16-v2.jpg
Download elife-81332-fig6-data16-v2.jpg
Figure 6—source data 17 Raw unlabeled non-parenchymal cells EMSA short exposure Figure 6D.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data17-v2.jpg
Download elife-81332-fig6-data17-v2.jpg
Figure 6—source data 18 Raw unlabeled non-parenchymal cells EMSA short exposure 2-ME Figure 6D.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data18-v2.jpg
Download elife-81332-fig6-data18-v2.jpg
Figure 6—source data 19 Raw unlabeled non-parenchymal cells EMSA long exposure Figure 6D.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data19-v2.jpg
Download elife-81332-fig6-data19-v2.jpg
Figure 6—source data 20 Raw unlabeled non-parenchymal cells EMSA long exposure 2-ME Figure 6D.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data20-v2.jpg
Download elife-81332-fig6-data20-v2.jpg
Figure 6—source data 21 Raw labeled liver EMSA long exposure Figure 6A.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data21-v2.bmp
Download elife-81332-fig6-data21-v2.bmp
Figure 6—source data 22 Raw labeled liver EMSA long exposure 2-ME Figure 6A.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data22-v2.bmp
Download elife-81332-fig6-data22-v2.bmp
Figure 6—source data 23 Raw labeled liver EMSA short exposure Figure 6A.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data23-v2.bmp
Download elife-81332-fig6-data23-v2.bmp
Figure 6—source data 24 Raw labeled liver EMSA short exposure 2-ME Figure 6A.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data24-v2.bmp
Download elife-81332-fig6-data24-v2.bmp
Figure 6—source data 25 Raw labeled spleen EMSA short exposure Figure 6B.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data25-v2.bmp
Download elife-81332-fig6-data25-v2.bmp
Figure 6—source data 26 Raw labeled spleen EMSA short exposure 2-ME Figure 6B.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data26-v2.bmp
Download elife-81332-fig6-data26-v2.bmp
Figure 6—source data 27 Raw labeled spleen EMSA long exposure Figure 6B.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data27-v2.bmp
Download elife-81332-fig6-data27-v2.bmp
Figure 6—source data 28 Raw labeled spleen EMSA long exposure 2-ME Figure 6B.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data28-v2.bmp
Download elife-81332-fig6-data28-v2.bmp
Figure 6—source data 29 Raw labeled hepatocytes EMSA short exposure Figure 6C.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data29-v2.bmp
Download elife-81332-fig6-data29-v2.bmp
Figure 6—source data 30 Raw labeled hepatocytes EMSA short exposure 2-ME Figure 6C.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data30-v2.bmp
Download elife-81332-fig6-data30-v2.bmp
Figure 6—source data 31 Raw labeled hepatocytes EMSA long exposure Figure 6C.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data31-v2.bmp
Download elife-81332-fig6-data31-v2.bmp
Figure 6—source data 32 Raw labeled hepatocytes EMSA long exposure 2-ME Figure 6C.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data32-v2.bmp
Download elife-81332-fig6-data32-v2.bmp
Figure 6—source data 33 Raw labeled non-parenchymal cells EMSA short exposure Figure 6D.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data33-v2.bmp
Download elife-81332-fig6-data33-v2.bmp
Figure 6—source data 34 Raw labeled non-parenchymal cells EMSA short exposure 2-ME Figure 6D.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data34-v2.bmp
Download elife-81332-fig6-data34-v2.bmp
Figure 6—source data 35 Raw labeled non-parenchymal cells EMSA long exposure Figure 6D.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data35-v2.bmp
Download elife-81332-fig6-data35-v2.bmp
Figure 6—source data 36 Raw labeled non-parenchymal cells EMSA long exposure 2-ME Figure 6D.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data36-v2.bmp
Download elife-81332-fig6-data36-v2.bmp
Figure 6—source data 37 Raw labeled Western blot ferroportin and β-actin Figure 6E.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig6-data37-v2.bmp
Download elife-81332-fig6-data37-v2.bmp

To clarify which cell types of the liver account for the responses of IRPs to dietary iron, separate EMSAs were performed using extracts from isolated hepatocytes or non-parenchymal liver cells. The data in Figure 6C–D uncover that IRP1 and IRP2 in both liver cell populations from wild type and Hjv^-/- mice are sensitive to dietary iron loading or restriction, respectively. The EMSA analysis of non-parenchymal liver cells, which contain Kupffer cells among others, showed a high experimental variability (Figure 6D). Nevertheless, the overall results are consistent with those obtained with splenic extracts, which contain red pulp macrophages (Figure 6B).

Relative expression of ferroportin in hepatocytes and non-parenchymal liver cells from wild type and Hjv^-/- mice

We determined the relative abundance of ferroportin in hepatocytes and non-parenchymal liver cells from wild type and Hjv^-/- mice on control diet by Western blotting. As expected, ferroportin expression (normalized to β-actin) was ~1.5–twofold higher in the non-parenchymal cell fraction as compared to hepatocytes in both wild type and Hjv^-/- mice (Figure 6E). In comparison across genotypes, ferroportin expression was ~2-fold higher in hepatocytes and ~50% higher in non-parenchymal cells from Hjv^-/- vs wild type mice.

Iron-dependent regulation of ferroportin mRNA translation in the liver

Having established that dietary iron manipulations trigger IRP responses in the liver and spleen, we hypothesized that the functional outcomes of exogenous hepcidin may not merely depend on its capacity to degrade tissue ferroportin but also on iron-dependent ferroportin regeneration via de novo synthesis. Slc40a1(+IRE) mRNA is the predominant ferroportin transcript in the mouse liver and spleen, as well as in hepatoma and macrophage cell lines (Zhang et al., 2009), and is considered as a target of IRPs.

Thus, we assessed the effects of dietary iron on whole liver Slc40a1(+IRE) mRNA translation by polysome profile analysis. We focused on the liver because this organ contains the highest number of iron-recycling macrophages (Krenkel and Tacke, 2017) and can also export iron to plasma from ferroportin-expressing parenchymal cells. Liver extracts from wild type mice on control diet or HID, and Hjv^-/- mice on control diet or IDD were fractionated on sucrose gradients to separate translationally inactive light monosomes from translating heavy polysomes (Figure 7A). The relative distribution of Slc40a1(+IRE), Fth1 (positive control for iron regulation), and Actb (negative control) mRNAs within the different fractions was quantified by qPCR (Figure 7B–D). Dietary iron loading stimulated Slc40a1(+IRE) (and Fth1) mRNA translation in wild type mice (note the shifts from monosomes to polysomes in Figure 7B–C). Conversely, dietary iron depletion inhibited Slc40a1(+IRE) (and Fth1) mRNA translation in Hjv^-/- mice. We also attempted to obtain polysome profiles of Slc40a1(-IRE) mRNA but it was undetectable after fractionation. These data indicate that in mice subjected to iron overload, iron-stimulated ferroportin synthesis in the liver antagonizes hepcidin-mediated ferroportin degradation and prevents an appropriate hypoferremic response. Considering that levels of Slc40a1(+IRE) mRNA are elevated in iron-loaded wild type and Hjv^-/- mice (Figure 2J and Figure 4—figure supplement 3), it is possible that increased de novo ferroportin synthesis is further enhanced by transcriptional induction.

Figure 7

Download asset Open asset

Iron regulation of *Slc40a1(+IRE)* mRNA translation in the mouse liver.

Four-week-old wild type male mice (n=10–14 per group) were placed on high-iron diet (HID) for 5 weeks. Conversely, age- and sex-matched isogenic *Hjv^-/-* mice (n=10–14 per group) were placed on iron-deficient diet (IDD) for 5 weeks to prevent excessive iron overload. Other animals from both genotypes were kept on control diet. At the endpoint, the mice were sacrificed, and livers were used for polysome profile analysis and iron assays. (A) Recording of absorbance at 254 nm of representative samples. Fraction numbers and direction of the gradient are indicated. (**B–D**) Liver polysome profiles from n=3 mice in each experimental group. Distribution of (B) *Slc40a1(+IRE*), (C) *Fth1* and (D) *Actb* mRNAs among light monosomal and heavy polysomal fractions (separated by dashed line) was analyzed by qPCR. Bar graph comparisons of pooled fractions are shown on the right. Numbers indicate the fold change compared towild type mice oncontrol diet. (**E and F**) Analysis of total iron (E), and redox iron speciation (F) in the liver by CE-ICP-MS. Data are presented as the mean ± SEM. Statistical analysis in (A) was performed by two-way ANOVA and in (**B, C**) by one-way ANOVA. Statistically significant differences (p<0.05) compared to values from wild type mice on control are indicated by a.

Figure 7—source data 1 Polysome raw data.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig7-data1-v2.xlsx
Download elife-81332-fig7-data1-v2.xlsx
Figure 7—source data 2 Iron ratio calculations.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig7-data2-v2.xlsx
Download elife-81332-fig7-data2-v2.xlsx

Quantification of liver iron by ICP-MS (Figure 7E) validated iron loading of wild type mice by HID, and relative iron depletion of Hjv^-/- mice by IDD intake, respectively (see also Figure 2D). Iron redox speciation analysis by CE-ICP-MS revealed a profound increase in Fe²⁺/Fe³⁺ ratios in livers of Hjv^-/- mice on control diet, which was corrected by dietary iron depletion (Figure 7F). Nevertheless, there was no difference in Fe²⁺/Fe³⁺ ratios among the livers of wild type mice on control diet or HID, and Hjv^-/- mice on IDD. We conclude that a relative increase in total iron content, rather than excessive accumulation of redox active Fe²⁺ drives Slc40a1(+IRE) (and Fth1) mRNA translation in the liver.

Restoration of effective hypoferremic response under iron overload following maximal Slc40a1 mRNA suppression

We reasoned that complete inactivation of ferroportin mRNA would restore hepcidin-induced hypoferremia despite iron overload. An 8 hr treatment of mice with LPS suppressed liver Slc40a1(+IRE) mRNA below detection levels (Figure 8A), as reported (Fillebeen et al., 2018). The same holds true for the Slc40a1(-IRE) isoform (Figure 8B), which was 290 times less abundant in control mouse livers compared to Slc40a1(+IRE) (ΔCt = 8.18), in agreement with published data (Zhang et al., 2009). We went on to examine the effects of synthetic hepcidin on serum iron under these conditions of maximal Slc40a1 mRNA suppression. Importantly, the prolonged LPS treatment decreased serum iron in wild type mice on HID below the control baseline (Figure 8C). Furthermore, when combined with synthetic hepcidin, it promoted an effective hypoferremic response in wild type mice on HID and Hjv^-/- mice on control diet (or IDD; Figure 8C–D) and tended to decrease NTBI (Figure 8E). These data strongly suggest that the expression of actively translating Slc40a1 mRNA in iron-exporting tissues under systemic iron overload mitigates the hepcidin-induced drop in serum iron.

Figure 8

Download asset Open asset

Elimination of ferroportin mRNA by prolonged LPS treatment potentiates hepcidin-induced hypoferremia in mouse models of iron overload.

Four-week-old wild type male mice (n=10–14 per group) were placed on high-iron diet (HID) for 5 weeks. Conversely, age- and sex-matched isogenic *Hjv^-/-* mice (n=10–14 per group) were placed on iron-deficient diet (IDD) for 5 weeks to prevent excessive iron overload. Other animals from both genotypes were kept on control diet. (**A and B**) Half of the mice were injected with saline and the other half with 1 µg/g LPS and sacrificed after 8 hr. Livers were dissected and processed for qPCR analysis of *Slc40a1(+IRE*) (A) and *Slc40a1(-IRE*) (B) mRNAs. (**C–E**) All mice were injected with 1 µg/g LPS. Half of the animals were subsequently injected with saline, and the other half with 2.5 µg/g synthetic hepcidin every 2 hr for a total of 4 injections. At the endpoint the mice were sacrificed. Sera were collected by cardiac puncture and analyzed for: (C) iron, (D) transferrin saturation, and (E) non-transferrin bound iron (NTBI). The dotted line in (C) and (D) indicates baseline serum iron and transferrin saturation, respectively, of wild type mice on control diet. Data are presented as (**A–B**) geometric mean ± SD or (**C–E**) mean ± SEM. Statistically significant differences (p<0.05) compared to values from saline-, LPS- or hepcidin-treated wild type mice on control diet are indicated by a, b or c, respectively.

Figure 8—source data 1 qPCR data.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig8-data1-v2.xlsx
Download elife-81332-fig8-data1-v2.xlsx
Figure 8—source data 2 Serum NTBI calculations.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig8-data2-v2.xlsx
Download elife-81332-fig8-data2-v2.xlsx
Figure 8—source data 3 Serum iron and transferrin saturation values.: https://cdn.elifesciences.org/articles/81332/elife-81332-fig8-data3-v2.xlsx
Download elife-81332-fig8-data3-v2.xlsx

Discussion

We sought to analyze how iron overload affects hepcidin-mediated inflammatory responses. We and others reported that excess iron inhibits the major hepcidin signaling pathways (BMP/SMAD and IL-6/STAT3) in cultured cells (Charlebois and Pantopoulos, 2021; Yu et al., 2021). To explore the physiological relevance of these findings, wild type mice were subjected to variable degrees of dietary iron loading and then treated with LPS. All iron-loaded mice could further upregulate hepcidin in response to LPS-induced acute inflammation (Figure 1). This is consistent with other relevant findings (Enculescu et al., 2017) and apparently contradicts the in vitro data. While experimental iron loading of cultured cells is rapid, dietary iron loading of mice is gradual (Daba et al., 2013) and most of the excess iron is effectively detoxified within ferritin, which is highly induced (Kent et al., 2015). By contrast, the suppression of hepcidin preceded ferritin induction in cultured cells (Charlebois and Pantopoulos, 2021), which may explain the discrepancy with the in vivo data.

The unimpaired inflammatory induction of hepcidin in iron-loaded wild type mice correlated with significant drops in serum iron, but these appeared inversely proportional to the degree of systemic iron loading (Figure 1). Thus, LPS-treated mice on 5 weeks of HID developed relative hypoferremia but could not further reduce serum iron below the baseline of untreated mice on control diet. This can be attributed to mechanisms antagonizing hepcidin action. To explore how iron modulates the capacity of hepcidin to trigger inflammatory hypoferremia, we established conditions of iron overload using wild type and Hjv^-/- mice with extreme differences in hepcidin expression. Figures 2 and 3 demonstrate that iron overload prevents effective inflammatory hypoferremia independently of hepcidin and tissue ferroportin levels.

We used a~200-fold excess of synthetic hepcidin to directly assess its capacity to divert iron traffic in iron-loaded mice. Hepcidin injection caused hypoferremia in wild type mice on control diet and significantly reduced serum iron in wild type mice on HID and Hjv^-/- mice on control diet, but not below baseline (Figure 4). Thus, synthetic hepcidin failed to drastically drop serum iron levels in iron overload models with either high or low endogenous hepcidin. Importantly, synthetic hepcidin promoted robust hypoferremia in relatively iron-depleted Hjv^-/- mice on IDD, with undetectable endogenous hepcidin. It should be noted that synthetic hepcidin had similar effects on tissue ferroportin among wild type or Hjv^-/- mice, regardless of iron diet (Figure 5). It reduced the intensity of the ferroportin signal in Kupffer cells and splenic macrophages of wild type mice without significantly affecting biochemically detectable total protein levels. In addition, it dramatically reduced the total ferroportin in the liver and spleen of Hjv^-/- mice. However, in all experimental settings, there was residual tissue ferroportin, which appears to be functionally significant.

We reasoned that at steady-state, tissue ferroportin may consist of fractions of newly synthesized protein and protein that is en route to hepcidin-mediated degradation. Conceivably, the former may exhibit more robust iron export activity, at least before its iron channel gets occluded by hepcidin. Increased de novo synthesis of active ferroportin could explain why synthetic hepcidin cannot drastically drop serum iron levels under iron overload. In fact, Figure 7 demonstrates that dietary iron overload augments Slc40a1(+IRE) mRNA translation in the liver of wild type mice. Conversely, relative dietary iron depletion inhibits Slc40a1(+IRE) mRNA translation in the liver of Hjv^-/- mice, in line with the restoration of hepcidin-mediated hypoferremic response (Figure 4).

Our data are consistent with translational control of liver ferroportin expression via the IRE/IRP system and do not exclude the possibility for an additional contribution of iron-dependent transcriptional regulation of Slc40a1(+IRE) mRNA. Direct evidence for activation of IRP responses in the liver and spleen to dietary iron manipulations is provided in Figure 6. While translational control of ferritin in tissues is established (Wilkinson and Pantopoulos, 2014), regulation of ferroportin by the IRE/IRP system is less well characterized and has hitherto only been documented in cell models (Lymboussaki et al., 2003; Nairz et al., 2015), the mouse duodenum (Galy et al., 2013), and the rat liver (Garza et al., 2020). Moreover, the physiological relevance of this mechanism remained speculative. The data in Figures 6 and 7 show that the IRE/IRP system is operational and controls Slc40a1(+IRE) mRNA translation in both fractions of hepatocytes and non-parenchymal liver cells. Presumably, this offers a compensatory mechanism to protect the cells from iron overload and iron-induced toxicity. On the other hand, this mechanism attenuates hepcidin responsiveness and promotes a state of hepcidin resistance, as higher amounts of hepcidin are required to achieve effective hypoferremia. Because hepcidin has a short plasma half-life, it is reasonable to predict that the use of more potent hepcidin analogs (Katsarou and Pantopoulos, 2018) will overcome the antagonistic effects of increased ferroportin mRNA translation under iron overload.

The critical role of de novo ferroportin synthesis in fine-tuning hepcidin-dependent functional outcomes is also highlighted in Figure 8. Thus, synthetic hepcidin was highly effective as a promoter of hypoferremia in dietary iron-loaded wild type mice when administered together with LPS. LPS is known to suppress Slc40a1 mRNA in cell lines (Ludwiczek et al., 2003) and mouse tissues, with a nadir in the liver reached at 8 hr (Fillebeen et al., 2018). The recovery of hepcidin effectiveness in mouse models of iron overload was only possible when Slc40a1 mRNA was essentially eliminated. Under these conditions, LPS treatment alone was sufficient to decrease serum iron in dietary iron-loaded wild type mice below baseline.

Tissue iron uptake may be another important determinant of the hypoferremic response to inflammation. LPS did not affect Tfrc mRNA levels in the liver (Figure 2O), which argues against increased uptake of transferrin-bound iron via Tfr1. On the other hand, LPS induced Slc39a14, Slc11a2, and Lcn2 mRNAs (Figure 2L–N). Zip14 is the NTBI transporter accounting for hepatocellular iron overload in hemochromatosis (Jenkitkasemwong et al., 2015) and is upregulated by inflammatory cues in hepatocytes (Liuzzi et al., 2005). DMT1 is dispensable for NTBI uptake by hepatocytes (Wang and Knutson, 2013), and its inflammatory induction might promote iron acquisition by macrophages (Ludwiczek et al., 2003; Wardrop and Richardson, 2000). Nevertheless, considering that the fraction of NTBI represents <2% of total serum iron even in the iron overload models (Figure 2A and C), it is implausible that NTBI uptake by Zip14 and/or DMT1 substantially contributes to inflammatory hypoferremia. Lcn2 is an acute phase protein that can sequester intracellular iron bound to catecholate siderophores (Xiao et al., 2017), and is more likely to transport iron to tissues during infection. In any case, synthetic hepcidin did not affect expression of iron transporters (Figure 4—figure supplement Figure 4—figure supplement 3E-H). This excludes the possibility for a synergistic effect on LPS-induced tissue iron uptake that could promote effective hypoferremia in the iron overload models.

Our study has some limitations. While the data highlight the importance of translational regulation of liver ferroportin as a determinant of serum iron, they do not accurately dissect the specific role of ferroportin expressed in hepatocytes and Kupffer cells; the latter were not separated from other non-parenchymal cells in biochemical assays. The involvement of the IRE/IRP system has been established indirectly, while the relative contributions of IRP1 and IRP2 in the mechanism are not fully defined. The possible role of iron-dependent transcriptional induction of ferroportin in counterbalancing hepcidin actions requires further clarification. The use of diets with variable iron content may have triggered responses to iron availability independent of hepcidin signaling and Hjv functionality. Finally, the physiological implications of translational regulation of ferroportin in the broader setting of inflammation and/or infection have not been explored.

In conclusion, our data reveal a crosstalk between the hepcidin pathway and the IRE/IRP system in the liver and spleen for the control of tissue ferroportin and serum iron levels. Furthermore, they suggest that application of hepcidin therapeutics for treatment of iron overload disorders should be combined with iron depletion strategies to mitigate Slc40a1 mRNA translation and increase hepcidin efficacy. Future work is expected to clarify whether optimizing the hypoferremic response to inflammation under systemic iron overload decreases susceptibility to pathogens.

Materials and methods

Animals

Wild type C57BL/6J and isogenic Hjv^-/- mice (Gkouvatsos et al., 2014) were housed in macrolone cages (up to 5 mice/cage, 12:12 hr light-dark cycle: 7 am–7 pm; 22 ± 1°C, 60 ± 5% humidity). The mice were fed either a standard control diet (200 ppm iron), an iron-deficient diet (2–6 ppm iron) or a high-iron diet (2% carbonyl iron) (Fillebeen et al., 2019). Where indicated, mice were injected intraperitoneally with 1 μg/g LPS (serotype 055:B5; Sigma-Aldrich) or subcutaneously with 2.5 µg/g synthetic hepcidin; control mice were injected with phosphate-buffered saline. At the endpoints, animals were sacrificed by CO₂ inhalation and cervical dislocation. Experimental procedures were approved by the Animal Care Committee of McGill University (protocol 4966).

Serum biochemistry

Request a detailed protocol

Blood was collected via cardiac puncture. Serum was prepared by using micro Z-gel tubes with clotting activator (Sarstedt) and was kept frozen at −20°C until analysis. Serum iron, total iron binding capacity (TIBC) and, where indicated serum ferritin, were determined at the Biochemistry Department of the Montreal Jewish General Hospital using a Roche Hitachi 917 Chemistry Analyzer. Transferrin saturation was calculated from the ratio of serum iron and TIBC. Serum hepcidin was measured by using an ELISA kit (HMC-001; Intrinsic LifeSciences).

Quantification of serum non-transferrin bound iron (NTBI)

Request a detailed protocol

NTBI was measured by adapting the method developed by Esposito et al (Esposito et al., 2003). Briefly, iron samples of known concentration were created by mixing 70 mM nitrilotriacetate (NTA) (pH = 7.0) with 20 mM ferrous ammonium sulfate. Fe²⁺ was allowed to oxidize to Fe³⁺ in ambient air for at least 30 min and then the solution was diluted to 0.2 mM before further serial dilutions to create a ladder. 5 μl of ladder was loaded in a 96-well plate containing 195 μl plasma-like medium with or without 100 μM deferiprone. The composition of the plasma-like medium was: 40 mg/ml bovine serum albumin, 1.2 mM sodium phosphate dibasic, 120 μM sodium citrate, 10 mM sodium bicarbonate in iron-free HEPES-buffered saline (HEPES 20 mM, NaCl 150 mM, treated with Chelex-100 chelating resin [Bio-Rad, Hercules, CA], 0.5 mM ΝΤΑ, 40 μM ascorbic acid, 50 μM dihydrorhodamine, pH = 7.4). 5 μl of sample was loaded in a 96-well plate containing 195 μl of iron-free HEPES-buffered saline with or without 100 μM deferiprone. Microplates were read every 2 min at 37℃ over 40 min at 485/520 nm (ex/em). Final ΝΤΒΙ was calculated by comparing the oxidation rate of DHR in the presence or absence of the strong chelator deferiprone.

Hepcidin synthesis

Request a detailed protocol

Human hepcidin (DTHFPICIFCCGCCHRSKCGMCCKT) was synthesized at Ferring Research Institute, San Diego, CA. The linear peptide was assembled on Rink amide resin using Tribute peptide synthesizer and the peptide was cleaved from the resin with the TFA/TIS/EDT/H₂O 91:3:3:3 (v/v/v/v) cocktail. The solvents were evaporated, and the crude peptide was precipitated with diethyl ether, reconstituted in 50% aqueous acetonitrile and lyophilized. The lyophilizate was dissolved in 30% aqueous acetonitrile at the concentration of 0.05 mM and the pH of the solution was adjusted to 7.8 with 6 M ammonium hydroxide. Folding was achieved within 4 hr using the cysteine/cystine redox (peptide/Cys/Cys₂ 1:6:6 molar ratio). The reaction mixture was acidified to pH 3, loaded onto HPLC prep column and purified in a TFA based gradient. The identity of the peptide was confirmed by mass spectrometry and by coelution with a commercially available sample (Peptide International, #PLP-3771-PI).

Quantitative real-time PCR (qPCR)

Request a detailed protocol

RNA was extracted from livers by using the RNeasy kit (Qiagen). cDNA was synthesized from 1 μg RNA by using the OneScript Plus cDNA Synthesis Kit (Applied Biological Materials Inc). Gene-specific primers pairs (Supplementary file 1) were validated by dissociation curve analysis and demonstrated amplification efficiency between 90–110%. SYBR Green (Bioline) and primers were used to amplify products under following cycling conditions: initial denaturation 95°C 10 min, 40 cycles of 95°C 5 s, 58°C 30 s, 72°C 10 s, and final cycle melt analysis between 58–95°C. Relative mRNA expression was calculated by the 2^-ΔΔCt method (Livak and Schmittgen, 2001). Data were normalized to murine ribosomal protein L19 (Rpl19). Data are reported as fold increases compared to samples from wild type mice on control diet.

Polysome fractionation

Request a detailed protocol

RNA was freshly prepared from frozen livers. Linear sucrose gradients were prepared the day before the experiment by using 5% (w/v) and 50% (w/v) sucrose solutions with 10 × gradient buffer (200 mM HEPES pH = 7.6, 1 M KCl, 50 mM MgCl₂, 0.1 mg/ml Cycloheximide, 1 tablet cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail (Roche), 200 U/mL Recombinant RNasin Ribonuclease Inhibitor (Promega), 2 mM DTT). Linear gradients were prepared in Polyallomer Centrifuge Tubes (Beckman Coulter). Tubes were marked using a gradient cylinder (BioComp), and 5% sucrose solution was added using a syringe with a layering needle (BioComp) until solution level reached the mark. Then, 50% sucrose solution was layered underneath the 5% solution until the interface between the two solutions reached the mark. Tubes were capped with rate zonal caps (BioComp) and linearized using a Gradient Master 108 (Biocomp). All reagents were nuclease-free and all solutions were kept on ice or at 4℃. Sample preparation was adapted from Liang et al., 2018. Briefly, livers were flash frozen upon collection. Roughly 30–80 mg of tissue was crushed using a mortar and pestle in the presence of liquid nitrogen to prevent thawing. Tissues were lysed in up to 1 ml of hypotonic lysis buffer (5 mM Tris-Hcl pH = 7.5, 1.5 mM KCl, 2.5 mM MgCl₂, 2 mM DTT, 1 mg/ml Cycloheximide, 200 U/ml Recombinant RNasin Ribonuclease Inhibitor [Promega], 1 tablet cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail [Roche] 0.5% [v/v] Triton X-100, 0.5% [v/v] Sodium Deoxycholate) and homogenized using Dounce homogenizers (60 movements with both loose and tight pestles) on ice. Samples were centrifuged at 4℃, 16,060 g for 4 min and supernatants were collected. Sample optical density was measured at 260 nM and samples were normalized to either the lowest value or 30 ODs. 450 μl of sucrose gradient was removed from the top and replaced with normalized sample. Tube weights were balanced by weight before centrifugation at 200,000 g for 2 hr at 4℃ in a SW 41 Ti rotor and a Beckman Optima L-60 Ultracentrifuge. Samples were fractionated using a BR-188 Density Gradient Fractionation System (Brandel). Immediately upon collection, 800 μl of samples were mixed with 1 ml of TRIzol and kept on ice before storage at –80℃. Polysomal RNA was processed according to the manufacturer’s protocol. mRNA distribution was analyzed as previously described (Panda et al., 2017).

Electrophoretic mobility shift assay (EMSA)

Request a detailed protocol

IRE-binding activities from liver and spleen were analyzed by EMSA using a radioactive ³²P-labelled IRE probe, according to established procedures (Fillebeen et al., 2014). EMSAs were also performed in extracts from hepatocytes and non-parenchymal cells, which were separated by using a 2-step collagenase perfusion technique, as previously described (Fillebeen et al., 2018).

Western blotting

Request a detailed protocol

Livers were washed with ice-cold PBS and dissected into pieces. Aliquots were snap frozen at liquid nitrogen and stored at −80°C. Protein lysates were obtained as described (Katsarou et al., 2021). Lysates containing 40 μg of proteins were analyzed by SDS-PAGE on 9–13% gels and proteins were transferred onto nitrocellulose membranes (BioRad). The blots were blocked in non-fat milk diluted in tris-buffered saline (TBS) containing 0.1% (v/v) Tween-20 (TBS-T), and probed overnight with antibodies against ferroportin (Ross et al., 2017; 1:1000 diluted monoclonal rat anti-mouse 1C7, kindly provided by Amgen Inc), β-actin (1:2000 diluted; Sigma), Tfr2 (1:1000 diluted rabbit polyclonal; Alpha Diagnostics), or Tfr1 (1:1000 diluted mouse monoclonal, Invitrogen). Following a 3 × wash with TBS-T, the membranes were incubated with peroxidase-coupled secondary antibodies for 1 hr. Immunoreactive bands were detected by enhanced chemiluminescence with the Western Lightning ECL Kit (Perkin Elmer).

Immunohistochemistry

Request a detailed protocol

Tissue specimens were fixed in 10% buffered formalin and embedded in paraffin. Samples from three different mice for each experimental condition were cut at 4 µm, placed on SuperFrost/Plus slides (Fisher) and dried overnight at 37°C. The slides were then loaded onto the Discovery XT Autostainer (Ventana Medical System) for automated immunohistochemistry. Slides underwent deparaffinization and heat-induced epitope retrieval. Immunostaining was performed by using 1:500 diluted rabbit polyclonal antibodies against ferroportin (Maffettone et al., 2010) and an appropriate detection kit (Omnimap rabbit polyclonal HRP, #760–4311 and ChromoMap-DAB #760–159; Roche). Negative controls were performed by the omission of the primary antibody. Slides were counterstained with hematoxylin for 4 min, blued with Bluing Reagent for 4 min, removed from the autostainer, washed in warm soapy water, dehydrated through graded alcohols, cleared in xylene, and mounted with Permount (Fisher). Sections were analyzed by conventional light microscopy and quantified by using the Aperio ImageScope software (Leica Biosystems; Fillebeen et al., 2018).

Perls Prussian blue staining

Request a detailed protocol

To visualize non-heme iron deposits, deparaffinized tissue sections were stained with Perls’ Prussian blue using the Accustain Iron Stain kit (Sigma).

Quantification of liver iron content (LIC)

Request a detailed protocol

Total liver iron was quantified by using the ferrozine assay (Daba et al., 2013) or inductively coupled plasma mass spectrometry (ICP-MS; Michalke et al., 2019).

Iron speciation analysis

Request a detailed protocol

Iron redox speciation analysis in the liver was performed by capillary electrophoresis (CE) coupled to ICP-MS (CE-ICP-MS). Dynamic reaction cell (DRC) technology (ICP-DRC-MS) with NH₃ as DRC-gas was applied for non-interfered monitoring of the iron isotopes. A ‘PrinCe 706’ CE system (PrinCe Technologies B.V., Emmen, Netherlands) was employed for separation of iron species at +20 kV. Temperature settings for sample/buffer tray and capillary were set to 20°C. An uncoated capillary (100 cm × 50 µm ID; CS-Chromatographie Service GmbH, Langerwehe, Germany) was used for separation and hyphenation to the ICP–DRC-MS. A CE-ICP-MS interface (Michalke et al., 2019; Michalke et al., 2020) was installed which provided the electrical connection between CE capillary end and outlet electrode. The self-aspiration mode allowed for best flow rate adjustment and avoided suction flow. Electrolytes for sample stacking and electrophoretic separation were 10% HCl = leading electrolyte, 0.05 mM HCl = terminating electrolyte and 50 mM HCl = background electrolyte. The Fe²⁺/Fe³⁺ ratio was calculated from quantitative determined concentrations of Fe-species.

Statistics

Statistical analysis was performed by using the Prism GraphPad software (version 9.1.0). Lognormally distributed data including qPCR and ELISA results were first log transformed before analysis with ordinary two-way ANOVA (Tukey’s multiple comparisons test) for comparisons within same treatment groups (denoted by a or b on figures) or with multiple unpaired t tests using the Holm-Sidak method to compare effects between treatments. Normally distributed data was analyzed by two-way ANOVA using either Sidak’s method for comparisons between treatment groups or Tukey’s multiple comparisons test within treatments groups. Where indicated, pairwise comparisons were done with unpaired Student’s t test. Probability value p<0.05 was considered statistically significant.

Data availability

All data generated or analysed during this study are included in the manuscript and supporting file.

References

1. Aschemeyer S
2. Qiao B
3. Stefanova D
4. Valore EV
5. Sek AC
6. Ruwe TA
7. Vieth KR
8. Jung G
9. Casu C
10. Rivella S
11. Jormakka M
12. Mackenzie B
13. Ganz T
14. Nemeth E
(2018) Structure-function analysis of ferroportin defines the binding site and an alternative mechanism of action of hepcidin
Blood 131:899–910.

https://doi.org/10.1182/blood-2017-05-786590
- PubMed
- Google Scholar
(2009) Iron loading increases ferroportin heterogeneous nuclear RNA and mrna levels in murine J774 macrophages
The Journal of Nutrition 139:434–438.

https://doi.org/10.3945/jn.108.094052
- PubMed
- Google Scholar
1. Billesbølle CB
2. Azumaya CM
3. Kretsch RC
4. Powers AS
5. Gonen S
6. Schneider S
7. Arvedson T
8. Dror RO
9. Cheng Y
10. Manglik A
(2020) Structure of hepcidin-bound ferroportin reveals iron homeostatic mechanisms
Nature 586:807–811.

https://doi.org/10.1038/s41586-020-2668-z
- PubMed
- Google Scholar
(2020) Iron metabolism and iron disorders revisited in the hepcidin era
Haematologica 105:260–272.

https://doi.org/10.3324/haematol.2019.232124
- PubMed
- Google Scholar
1. Charlebois E
2. Pantopoulos K
(2021) Iron overload inhibits BMP/SMAD and IL-6/STAT3 signaling to hepcidin in cultured hepatocytes
PLOS ONE 16:e0253475.

https://doi.org/10.1371/journal.pone.0253475
- PubMed
- Google Scholar
1. Corradini E
2. Meynard D
3. Wu Q
4. Chen S
5. Ventura P
6. Pietrangelo A
7. Babitt JL
(2011) Serum and liver iron differently regulate the bone morphogenetic protein 6 (BMP6)-SMAD signaling pathway in mice
Hepatology 54:273–284.

https://doi.org/10.1002/hep.24359
- PubMed
- Google Scholar
(2013) Differences in activation of mouse hepcidin by dietary iron and parenterally administered iron dextran: compartmentalization is critical for iron sensing
Journal of Molecular Medicine 91:95–102.

https://doi.org/10.1007/s00109-012-0937-5
- PubMed
- Google Scholar
1. Deschemin JC
2. Vaulont S
(2013) Role of hepcidin in the setting of hypoferremia during acute inflammation
PLOS ONE 8:e61050.

https://doi.org/10.1371/journal.pone.0061050
- PubMed
- Google Scholar
(2015) Ironing out ferroportin
Cell Metabolism 22:777–787.

https://doi.org/10.1016/j.cmet.2015.09.006
- PubMed
- Google Scholar
(2017) Modelling systemic iron regulation during dietary iron overload and acute inflammation: role of hepcidin-independent mechanisms
PLOS Computational Biology 13:e1005322.

https://doi.org/10.1371/journal.pcbi.1005322
- PubMed
- Google Scholar
(2003) Labile plasma iron in iron overload: redox activity and susceptibility to chelation
Blood 102:2670–2677.

https://doi.org/10.1182/blood-2003-03-0807
- PubMed
- Google Scholar
(2014) Electrophoretic mobility shift assay (EMSA) for the study of RNA-protein interactions: the IRE/IRP example
Journal of Visualized Experiments 2014:94.

https://doi.org/10.3791/52230
- PubMed
- Google Scholar
(2018) Hepcidin-mediated hypoferremic response to acute inflammation requires a threshold of bmp6/hjv/smad signaling
Blood 132:1829–1841.

https://doi.org/10.1182/blood-2018-03-841197
- PubMed
- Google Scholar
1. Fillebeen C
2. Charlebois E
3. Wagner J
4. Katsarou A
5. Mui J
6. Vali H
7. Garcia-Santos D
8. Ponka P
9. Presley J
10. Pantopoulos K
(2019) Transferrin receptor 1 controls systemic iron homeostasis by fine-tuning hepcidin expression to hepatocellular iron load
Blood 133:344–355.

https://doi.org/10.1182/blood-2018-05-850404
- PubMed
- Google Scholar
1. Galy B
2. Ferring-Appel D
3. Becker C
4. Gretz N
5. Gröne HJ
6. Schümann K
7. Hentze MW
(2013) Iron regulatory proteins control a mucosal block to intestinal iron absorption
Cell Reports 3:844–857.

https://doi.org/10.1016/j.celrep.2013.02.026
- PubMed
- Google Scholar
1. Ganz T
2. Nemeth E
(2015) Iron homeostasis in host defence and inflammation
Nature Reviews. Immunology 15:500–510.

https://doi.org/10.1038/nri3863
- PubMed
- Google Scholar
(2020) Differential translational control of 5’ IRE-containing mrna in response to dietary iron deficiency and acute iron overload
Metallomics 12:2186–2198.

https://doi.org/10.1039/d0mt00192a
- PubMed
- Google Scholar
(2014) Iron-dependent regulation of hepcidin in hjv-/- mice: evidence that hemojuvelin is dispensable for sensing body iron levels
PLOS ONE 9:e85530.

https://doi.org/10.1371/journal.pone.0085530
- PubMed
- Google Scholar
1. Guida C
2. Altamura S
3. Klein FA
4. Galy B
5. Boutros M
6. Ulmer AJ
7. Hentze MW
8. Muckenthaler MU
(2015) A novel inflammatory pathway mediating rapid hepcidin-independent hypoferremia
Blood 125:2265–2275.

https://doi.org/10.1182/blood-2014-08-595256
- PubMed
- Google Scholar
(2005) A mouse model of juvenile hemochromatosis
The Journal of Clinical Investigation 115:2187–2191.

https://doi.org/10.1172/JCI25049
- PubMed
- Google Scholar
1. Jenkitkasemwong S
2. Wang CY
3. Coffey R
4. Zhang W
5. Chan A
6. Biel T
7. Kim JS
8. Hojyo S
9. Fukada T
10. Knutson MD
(2015) SLC39A14 is required for the development of hepatocellular iron overload in murine models of hereditary hemochromatosis
Cell Metabolism 22:138–150.

https://doi.org/10.1016/j.cmet.2015.05.002
- PubMed
- Google Scholar
1. Johnson MB
2. Enns CA
(2004) Diferric transferrin regulates transferrin receptor 2 protein stability
Blood 104:4287–4293.

https://doi.org/10.1182/blood-2004-06-2477
- PubMed
- Google Scholar
1. Katsarou A
2. Pantopoulos K
(2018) Hepcidin therapeutics
Pharmaceuticals 11:E127.

https://doi.org/10.3390/ph11040127
- PubMed
- Google Scholar
1. Katsarou A
2. Pantopoulos K
(2020) Basics and principles of cellular and systemic iron homeostasis
Molecular Aspects of Medicine 75:100866.

https://doi.org/10.1016/j.mam.2020.100866
- PubMed
- Google Scholar
(2021) Tissue-specific regulation of ferroportin in wild-type and hjv-/- mice following dietary iron manipulations
Hepatology Communications 5:2139–2150.

https://doi.org/10.1002/hep4.1780
- PubMed
- Google Scholar
1. Kent P
2. Wilkinson N
3. Constante M
4. Fillebeen C
5. Gkouvatsos K
6. Wagner J
7. Buffler M
8. Becker C
9. Schümann K
10. Santos MM
11. Pantopoulos K
(2015) Hfe and hjv exhibit overlapping functions for iron signaling to hepcidin
Journal of Molecular Medicine 93:489–498.

https://doi.org/10.1007/s00109-015-1253-7
- PubMed
- Google Scholar
1. Krenkel O
2. Tacke F
(2017) Liver macrophages in tissue homeostasis and disease
Nature Reviews. Immunology 17:306–321.

https://doi.org/10.1038/nri.2017.11
- PubMed
- Google Scholar
(2015) Functions and development of red pulp macrophages
Microbiology and Immunology 59:55–62.

https://doi.org/10.1111/1348-0421.12228
- PubMed
- Google Scholar
1. Laftah AH
2. Ramesh B
3. Simpson RJ
4. Solanky N
5. Bahram S
6. Schümann K
7. Debnam ES
8. Srai SKS
(2004) Effect of hepcidin on intestinal iron absorption in mice
Blood 103:3940–3944.

https://doi.org/10.1182/blood-2003-03-0953
- PubMed
- Google Scholar
1. Liang S
2. Bellato HM
3. Lorent J
4. Lupinacci FCS
5. Oertlin C
6. van Hoef V
7. Andrade VP
8. Roffé M
9. Masvidal L
10. Hajj GNM
11. Larsson O
(2018) Polysome-profiling in small tissue samples
Nucleic Acids Research 46:e3.

https://doi.org/10.1093/nar/gkx940
- PubMed
- Google Scholar
1. Liuzzi JP
2. Lichten LA
3. Rivera S
4. Blanchard RK
5. Aydemir TB
6. Knutson MD
7. Ganz T
8. Cousins RJ
(2005) Interleukin-6 regulates the zinc transporter zip14 in liver and contributes to the hypozincemia of the acute-phase response
PNAS 102:6843–6848.

https://doi.org/10.1073/pnas.0502257102
- PubMed
- Google Scholar
1. Livak KJ
2. Schmittgen TD
(2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-delta delta c(t)) method
Methods 25:402–408.

https://doi.org/10.1006/meth.2001.1262
- PubMed
- Google Scholar
1. Ludwiczek S
2. Aigner E
3. Theurl I
4. Weiss G
(2003) Cytokine-mediated regulation of iron transport in human monocytic cells
Blood 101:4148–4154.

https://doi.org/10.1182/blood-2002-08-2459
- PubMed
- Google Scholar
(2003) The role of the iron responsive element in the control of ferroportin1/IREG1/MTP1 gene expression
Journal of Hepatology 39:710–715.

https://doi.org/10.1016/s0168-8278(03)00408-2
- PubMed
- Google Scholar
(2010) Tumorigenic properties of iron regulatory protein 2 (IRP2) mediated by its specific 73-amino acids insert
PLOS ONE 5:e10163.

https://doi.org/10.1371/journal.pone.0010163
- PubMed
- Google Scholar
(2019) Iron redox speciation analysis using capillary electrophoresis coupled to inductively coupled plasma mass spectrometry (CE-ICP-MS)
Frontiers in Chemistry 7:136.

https://doi.org/10.3389/fchem.2019.00136
- PubMed
- Google Scholar
(2020) Setup of capillary electrophoresis-inductively coupled plasma mass spectrometry (CE-ICP-MS) for quantification of iron redox species (fe(II), fe(iii))
Journal of Visualized Experiments 2020:159.

https://doi.org/10.3791/61055
- PubMed
- Google Scholar
(2008) Systemic iron homeostasis and the iron-responsive element/iron-regulatory protein (IRE/IRP) regulatory network
Annual Review of Nutrition 28:197–213.

https://doi.org/10.1146/annurev.nutr.28.061807.155521
- PubMed
- Google Scholar
1. Nairz M
2. Ferring-Appel D
3. Casarrubea D
4. Sonnweber T
5. Viatte L
6. Schroll A
7. Haschka D
8. Fang FC
9. Hentze MW
10. Weiss G
11. Galy B
(2015) Iron regulatory proteins mediate host resistance to salmonella infection
Cell Host & Microbe 18:254–261.

https://doi.org/10.1016/j.chom.2015.06.017
- PubMed
- Google Scholar
(2017) Polysome fractionation to analyze mrna distribution profiles
BIO-PROTOCOL 7:e2126.

https://doi.org/10.21769/BioProtoc.2126
- PubMed
- Google Scholar
1. Ross SL
2. Biswas K
3. Rottman J
4. Allen JR
5. Long J
6. Miranda LP
7. Winters A
8. Arvedson TL
(2017) Identification of antibody and small molecule antagonists of ferroportin-hepcidin interaction
Frontiers in Pharmacology 8:838.

https://doi.org/10.3389/fphar.2017.00838
- PubMed
- Google Scholar
1. Schmidt PJ
(2015) Regulation of iron metabolism by hepcidin under conditions of inflammation
The Journal of Biological Chemistry 290:18975–18983.

https://doi.org/10.1074/jbc.R115.650150
- PubMed
- Google Scholar
1. Schulze RJ
2. Schott MB
3. Casey CA
4. Tuma PL
5. McNiven MA
(2019) The cell biology of the hepatocyte: A membrane trafficking machine
The Journal of Cell Biology 218:2096–2112.

https://doi.org/10.1083/jcb.201903090
- PubMed
- Google Scholar
1. Wang J
2. Pantopoulos K
(2011) Regulation of cellular iron metabolism
The Biochemical Journal 434:365–381.

https://doi.org/10.1042/BJ20101825
- PubMed
- Google Scholar
1. Wang CY
2. Knutson MD
(2013) Hepatocyte divalent metal-ion transporter-1 is dispensable for hepatic iron accumulation and non-transferrin-bound iron uptake in mice
Hepatology 58:788–798.

https://doi.org/10.1002/hep.26401
- PubMed
- Google Scholar
1. Wang CY
2. Babitt JL
(2019) Liver iron sensing and body iron homeostasis
Blood 133:18–29.

https://doi.org/10.1182/blood-2018-06-815894
- PubMed
- Google Scholar
1. Wardrop SL
2. Richardson DR
(2000) Interferon-gamma and lipopolysaccharide regulate the expression of nramp2 and increase the uptake of iron from low relative molecular mass complexes by macrophages
European Journal of Biochemistry 267:6586–6593.

https://doi.org/10.1046/j.1432-1327.2000.01752.x
- PubMed
- Google Scholar
1. Wilkinson N
2. Pantopoulos K
(2014) The IRP/IRE system in vivo: insights from mouse models
Frontiers in Pharmacology 5:176.

https://doi.org/10.3389/fphar.2014.00176
- PubMed
- Google Scholar
(2017) Lipocalin 2: an emerging player in iron homeostasis and inflammation
Annual Review of Nutrition 37:103–130.

https://doi.org/10.1146/annurev-nutr-071816-064559
- PubMed
- Google Scholar
1. Yu LN
2. Wang SJ
3. Chen C
4. Rausch V
5. Elshaarawy O
6. Mueller S
(2021) Direct modulation of hepatocyte hepcidin signaling by iron
World Journal of Hepatology 13:1378–1393.

https://doi.org/10.4254/wjh.v13.i10.1378
- PubMed
- Google Scholar
(2009) A ferroportin transcript that lacks an iron-responsive element enables duodenal and erythroid precursor cells to evade translational repression
Cell Metabolism 9:461–473.

https://doi.org/10.1016/j.cmet.2009.03.006
- PubMed
- Google Scholar

Article and author information

Author details

Edouard Charlebois

Lady Davis Institute for Medical Research, Jewish General Hospital and Department of Medicine, McGill University, Montreal, Canada

Contribution
Investigation, Methodology

Competing interests
No competing interests declared
Carine Fillebeen

Lady Davis Institute for Medical Research, Jewish General Hospital and Department of Medicine, McGill University, Montreal, Canada

Contribution
Investigation, Methodology, Project administration

Competing interests
No competing interests declared
Angeliki Katsarou

Lady Davis Institute for Medical Research, Jewish General Hospital and Department of Medicine, McGill University, Montreal, Canada

Contribution
Investigation

Competing interests
No competing interests declared
Aleksandr Rabinovich

Ferring Research Institute Inc, San Diego, United States

Contribution
Resources

Competing interests
Was an employee of Ferring Research Institute Inc
Kazimierz Wisniewski

Ferring Research Institute Inc, San Diego, United States

Contribution
Resources

Competing interests
Was an employee of Ferring Research Institute Inc
Vivek Venkataramani
1. Department of Medicine II, Hematology/Oncology, University Hospital Frankfurt, Frankfurt, Germany
2. Institute of Pathology, University Medical Center Göttingen (UMG), Göttingen, Germany
Contribution
Methodology

Competing interests
No competing interests declared
Bernhard Michalke

Helmholtz Zentrum München GmbH – German Research Center for Environmental Health, Research Unit Analytical BioGeoChemistry, Neuherberg, Germany

Contribution
Methodology

Competing interests
No competing interests declared
Anastasia Velentza

Ferring Research Institute Inc, San Diego, United States

Contribution
Resources

Competing interests
Was an employee of Ferring Research Institute Inc
Kostas Pantopoulos

Lady Davis Institute for Medical Research, Jewish General Hospital and Department of Medicine, McGill University, Montreal, Canada

Contribution
Conceptualization, Supervision, Funding acquisition, Writing - original draft, Writing - review and editing

For correspondence
kostas.pantopoulos@mcgill.ca

Competing interests
No competing interests declared

"This ORCID iD identifies the author of this article:" 0000-0002-2305-0057

Funding

Canadian Institutes of Health Research (PJT-159730)

Kostas Pantopoulos

Fonds de Recherche du Québec - Santé

Edouard Charlebois

Deutsche Forschungsgemeinschaft (SPP 2306)

Vivek Venkataramani

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Acknowledgements

We thank Dr. Naciba Benlimame and Lilian Canetti for assistance with histology and immunohistochemistry. This work was supported by a grant from the Canadian Institutes of Health Research (CIHR; PJT-159730). EC was funded by a fellowship from the Natural Sciences and Engineering Research Council of Canada (NSERC) and is currently a recipient of a fellowship from the Fonds de recherche du Québec – Santé (FRQS). The work of VV and BM was financially supported by the Deutsche Forschungsgemeinschaft (DFG) through the Priority Program “Ferroptosis: from Molecular Basics to Clinical Applications” (SPP 2306).

Ethics

All experimental procedures were approved by the Animal Care Committee of McGill University (protocol 4966).

Copyright

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

Metrics

1,134

views
285

downloads
9

citations

Views, downloads and citations are aggregated across all versions of this paper published by eLife.

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Open citations (links to open the citations from this article in various online reference manager services)

Mendeley

Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)

Edouard Charlebois
Carine Fillebeen
Angeliki Katsarou
Aleksandr Rabinovich
Kazimierz Wisniewski
Vivek Venkataramani
Bernhard Michalke
Anastasia Velentza
Kostas Pantopoulos

(2022)

A crosstalk between hepcidin and IRE/IRP pathways controls ferroportin expression and determines serum iron levels in mice

eLife 11:e81332.

https://doi.org/10.7554/eLife.81332

Categories and tags

Research organism

Mouse

Share this article

Cite this article

Dietary iron loading does not disrupt inflammatory hepcidin induction in LPS-treated wild type mice but blunts hepcidin-mediated hypoferremia.

Figure 1—source data 1

Figure 1—source data 2

Figure 1—source data 3

Figure 1—source data 4

Iron overload blunts hepcidin responsiveness to LPS-induced inflammation.

Figure 2—source data 1

Figure 2—source data 2

Figure 2—source data 3

Figure 2—source data 4

Figure 2—source data 5

Figure 2—source data 6

Effects of LPS on hepatic and splenic ferroportin of iron-manipulated wild type and Hjv-/- mice.

Figure 3—source data 1

Figure 3—source data 2

Figure 3—source data 3

Figure 3—source data 4

Figure 3—source data 5

Figure 3—source data 6

Figure 3—source data 7

Figure 3—source data 8

Figure 3—source data 9

Figure 3—source data 10

Figure 3—source data 11

Figure 3—source data 12

Figure 3—source data 13

Figure 3—source data 14

Figure 3—source data 15

Figure 3—source data 16

Figure 3—source data 17

Figure 3—source data 18

Figure 3—source data 19

Iron depletion of Hjv-/- mice improves the efficacy of synthetic hepcidin to promote hypoferremia.

Figure 4—source data 1

Figure 4—source data 2

Figure 4—source data 3

Figure 4—source data 4

Figure 4—source data 5

Figure 4—source data 6

Effects of synthetic hepcidin on hepatic and splenic ferroportin of iron-manipulatedwild type and Hjv-/- mice.

Figure 5—source data 1

Figure 5—source data 2

Figure 5—source data 3

Figure 5—source data 4

Figure 5—source data 5

Figure 5—source data 6

Figure 5—source data 7

Figure 5—source data 8

Figure 5—source data 9

Figure 5—source data 10

Figure 5—source data 11

Figure 5—source data 12

Figure 5—source data 13

Figure 5—source data 14

Figure 5—source data 15

Figure 5—source data 16

Figure 5—source data 17

Figure 5—source data 18

Figure 5—source data 19

Figure 5—source data 20

Figure 5—source data 21

Figure 5—source data 22

Figure 5—source data 23

Dietary iron manipulations trigger IRP responses in the liver and spleen, as well as in primary hepatocytes and non-parenchymal liver cells of wild type and Hjv-/- mice.

Figure 6—source data 1

Figure 6—source data 2

Figure 6—source data 3

Figure 6—source data 4

Figure 6—source data 5

Figure 6—source data 6

Figure 6—source data 7

Figure 6—source data 8

Figure 6—source data 9

Figure 6—source data 10

Figure 6—source data 11

Figure 6—source data 12

Figure 6—source data 13

Figure 6—source data 14

Effects of LPS on hepatic and splenic ferroportin of iron-manipulated wild type and Hjv^-/- mice.

Iron depletion of Hjv^-/- mice improves the efficacy of synthetic hepcidin to promote hypoferremia.

Effects of synthetic hepcidin on hepatic and splenic ferroportin of iron-manipulatedwild type and Hjv^-/- mice.

Dietary iron manipulations trigger IRP responses in the liver and spleen, as well as in primary hepatocytes and non-parenchymal liver cells of wild type and Hjv^-/- mice.