CD8+ tissue-resident memory T cells induce oral lichen planus erosion via cytokine network

Abstract

CD8+ tissue-resident memory T (CD8+ Trm) cells play key roles in many immune-inflammation-related diseases. However, their characteristics in the pathological process of oral lichen planus (OLP) remains unclear. Therefore, we investigated the function of CD8+ Trm cells in the process of OLP. By using single-cell RNA sequencing profiling and spatial transcriptomics, we revealed that CD8+ Trm cells were predominantly located in the lamina propria adjacent to the basement membrane and were significantly increased in patients with erosive oral lichen planus (EOLP) compared to those with non-erosive OLP (NEOLP). Furthermore, these cells displayed enhanced cytokine production, including IFN-γ, TNF-α, and IL17, in patients with EOLP. And our clinical cohort of 1-year follow-up was also supported the above results in RNA level and protein level. In conclusion, our study provided a novel molecular mechanism for triggering OLP erosion by CD8+ Trm cells to secrete multiple cytokines, and new insight into the pathological development of OLP.

Data availability

The data of this study, including scRNA-seq data, ST data, and bulk RNA-seq data are available in the Gene Expression Omnibus (GEO) database, accession numbers GSE213345, GSE213346 and GSE211630.

The following data sets were generated

Article and author information

Author details

  1. Maofeng Qing

    State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-7829-5564
  2. Dan Yang

    State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China
    Competing interests
    The authors declare that no competing interests exist.
  3. Qianhui Shang

    State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China
    Competing interests
    The authors declare that no competing interests exist.
  4. Jiakuan Peng

    State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China
    Competing interests
    The authors declare that no competing interests exist.
  5. Jiaxin Deng

    State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China
    Competing interests
    The authors declare that no competing interests exist.
  6. Lu Jiang

    State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China
    Competing interests
    The authors declare that no competing interests exist.
  7. Jing Li

    State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China
    Competing interests
    The authors declare that no competing interests exist.
  8. HongXia Dan

    State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China
    Competing interests
    The authors declare that no competing interests exist.
  9. Yu Zhou

    State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China
    For correspondence
    812471898@qq.com
    Competing interests
    The authors declare that no competing interests exist.
  10. Hao Xu

    State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China
    For correspondence
    hao.xu@scu.edu.cn
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-5665-0139
  11. Qianming Chen

    State Key Laboratory of Oral Diseases, Zhejiang University, Hangzhou, China
    For correspondence
    qmchen@scu.edu.cn
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-5371-4432

Funding

National Natural Science Foundation of China (81730030)

  • Qianming Chen

National Natural Science Foundation of China (81730030)

  • Hao Xu

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Brian S Kim MD, Icahn School of Medicine at Mount Sinai, United States

Ethics

Human subjects: All individuals provided written informed consent and this study was supported by the Ethics Committee of West China Hospital of Stomatology Sichuan University [WCHSIRB-2019-167].

Version history

  1. Received: October 5, 2022
  2. Preprint posted: October 19, 2022 (view preprint)
  3. Accepted: July 25, 2023
  4. Accepted Manuscript published: August 9, 2023 (version 1)
  5. Version of Record published: August 29, 2023 (version 2)

Copyright

© 2023, Qing et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Maofeng Qing
  2. Dan Yang
  3. Qianhui Shang
  4. Jiakuan Peng
  5. Jiaxin Deng
  6. Lu Jiang
  7. Jing Li
  8. HongXia Dan
  9. Yu Zhou
  10. Hao Xu
  11. Qianming Chen
(2023)
CD8+ tissue-resident memory T cells induce oral lichen planus erosion via cytokine network
eLife 12:e83981.
https://doi.org/10.7554/eLife.83981

Share this article

https://doi.org/10.7554/eLife.83981

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